Effects of chronic parenteral pyridoxine and acute enteric tryptophan on pyridoxine status, glycemia and insulinemia stimulated by enteric glucose in weanling piglets

1997 ◽  
Vol 77 (4) ◽  
pp. 663-668 ◽  
Author(s):  
J. J. Matte ◽  
A. A. Ponter ◽  
B. Sève
Keyword(s):  
Insulin Response ◽  
Reliable Estimate ◽  
Large White ◽  
Glucose Response ◽  
Insulin Metabolism ◽  
Intraduodenal Infusion ◽  
Treatment Groups ◽  
Liquid Feed ◽  
Insulin Response To Glucose ◽  
Weanling Piglets

In order to investigate the importance of the relation between pyridoxine and tryptophan on glucose tolerance and insulin response to glucose, 12 Large White × Pietrain piglets (males and females), weaned at 3 wk of age, were allocated to two treatment groups. Within each of the six pairs (four females and two males), one piglet received daily 3 mL i.m. of pyridoxine.HCl (5 g L−1) and the other received a control injection of saline. The animals were fed a liquid feed through a gastric tube surgically inserted on the day of weaning. Seven days later, one catheter was placed in the duodenum and another in the jugular vein. One week after recovery, the piglets received an intraduodenal infusion of glucose or glucose + tryptophan; 2 d later, each piglet received the opposite treatment. There was no effect (P > 0.18) of the administration of pyridoxine.HCl on plasma pyridoxal and pyridoxal-5-phosphate. Whatever the pyridoxine treatment, the plasma glucose response was lower (P ≤ 0.05) after the glucose + tryptophan infusion than after the glucose infusion. There was, also, an interaction between parenteral pyridoxine and duodenal infusion on changes in plasma insulin concentrations following the duodenal infusion (P ≤ 0.02). The greatest response, observed in piglets supplemented with pyridoxine.HCl and infused with glucose, was 55% higher than for the three other treatments. Further work is needed for an eventual reliable estimate of the pyridoxine status and requirements of weanling piglets. The tryptophan and pyridoxine effects on insulin metabolism suggest a different action of these nutrients on sensitivity and release of insulin. Key words: Pyridoxine, tryptophan, glucose, insulin, weanling piglets

Effects of dam parity and rearing rank on the glucose and fat metabolism, and adrenal function of post-pubertal single and twin-ewe progeny

2010 ◽  
Vol 50 (6) ◽  
pp. 473 ◽  
Author(s):  
S. J. Pain ◽  
P. R. Kenyon ◽  
S. T. Morris ◽  
H. T. Blair
Keyword(s):  
Fat Metabolism ◽  
Plasma Concentrations ◽  
Insulin Response ◽  
Adrenal Function ◽  
Glucose Response ◽  
Insulin Metabolism ◽  
Intravenous Glucose ◽  
Glucose Challenge ◽  
Basal Plasma ◽  
Ewe Lamb

In an effort to increase the number of lambs produced per ewe’s productive lifetime in New Zealand, an increasing number of ewe lambs (8–9 months old) are being bred. This, in turn, results in an increased proportion of second-parity 2-year-old ewes in New Zealand’s breeding flock, rather than the more usual first-parity 2-year olds. The longer-term effects of dam parity on resulting ewe progeny are of interest and few studies have examined this. The present study was designed to determine whether parity (first or second) of Romney 2-year-old dams had any effect on the metabolic function of their single- and twin-born and reared ewe lamb progeny at 10 months of age. Ten-month-old, single and twin ewe lamb progeny born to first- or second-parity dams (n = 8 per group) were catheterised and given intravenous glucose (0.17 g/kg liveweight) (GTT), insulin (0.15 IU/kg liveweight) (ITT) and epinephrine (1 μg/kg liveweight) (ETT) tolerance tests to assess their glucose and fat metabolism and adrenal function. Rearing rank reduced (P < 0.05) the insulin response of twins to a glucose challenge, but increased (P < 0.05) their glucose response to an insulin challenge. Offspring from first-parity dams had higher (P < 0.05) basal plasma concentrations of cortisol and cortisone, whereas their cortisol/cortisone responses to an insulin challenge were unaffected by either dam parity or rearing rank. Neither dam parity nor rearing rank appeared to influence responses to an epinephrine challenge. The present study suggests that both dam parity and rearing rank alter the glucose and insulin metabolism of the offspring, which may have longer-term impacts on the growth and reproductive efficiency of the animal.

Influence of energy and protein concentration in the diet on the performance of growing pigs 2. Differing nutrient density at a constant energy: protein ratio

1972 ◽  
Vol 14 (1) ◽  
pp. 47-55 ◽  
Author(s):  
G. A. Lodge ◽  
M. E. Cundy ◽  
R. Cooke ◽  
D. Lewis
Keyword(s):  
Growth Rate ◽  
Live Weight ◽  
Fat Content ◽  
Growing Pigs ◽  
Feed Conversion ◽  
Large White ◽  
Protein Ratio ◽  
Treatment Groups ◽  
Nutrient Density ◽  
Amino Acid Balance

SUMMARYForty-eight gilts by Landrace sires on Large White × Landrace females were randomly allocated to eight pens and within pens to six treatment groups involving three diets and two levels of feeding from 23 to 59 kg live weight. All diets were formulated to have approximately the same ratio of digestible energy to crude protein (160 kcal DE/unit % CP) but different energy and protein concentrations: (A) 3500 kcal/kg DE and 21 % CP, (B) 3150 kcal/kg DE and 19% CP, and (C) 2800 kcal/kg DE and 17% CP. Amino acid balance was maintained relatively constant with synthetic lysine, methionine and tryptophan. The levels of feeding were such that the lower level of diet A allowed an intake of energy and protein similar to the higher level of diet B, and the lower level of B was similar to the higher level of C.On the lower level of feeding, growth rate, efficiency of feed conversion and carcass fat content increased linearly with each increment in nutrient concentration; on the higher level of feeding growth rate and EFC increased from diet C to B but not from B to A, whereas carcass fat content increased linearly with diet from the lowest to the highest concentration. There was a non-significant tendency for the higher density diets at a similar level of nutrient intake to give better EFC and fatter carcasses than the lower density diets.

The influence of intake of dietary energy in pregnancy and lactation upon sow productivity

1968 ◽  
Vol 71 (2) ◽  
pp. 215-222 ◽  
Author(s):  
F. W. H. Elsley ◽  
R. M. MacPherson ◽  
I. McDonald
Keyword(s):  
Daily Intake ◽  
Dietary Energy ◽  
Large White ◽  
Treatment Groups ◽  
Daily Energy ◽  
Reproductive Life ◽  
Pregnancy And Lactation ◽  
Daily Intakes ◽  
Practical Implications ◽  
In Pregnancy

SUMMARYFifty-two Large White gilts, arranged in thirteen groups of four litter sisters, were allocated at mating to one of four treatment groups. These treatments, arranged as a 2 × 2 factorial, allowed daily energy intakes in pregnancy of 8·3 Meal (H(e)) or 5·2 Meal (L(e)) and of average daily intake during lactation of 20 Meal (h(e)) or 13·8 Meal (m(e)). Daily intakes of protein, minerals and vitamins were similar for all treatments. There were no consistent differences in health or breeding regularity between the treatments.The H(e) sows had a net gain in pregnancy 22 kg greater than the L(e) sows. Feeding the higher level in lactation (h(e) resulted in a reduced sow weight loss during lactation of 20, 12 and 10 kg in the first, second and third parities respectively, as compared with the m(e) sows. There were no consistent effects on the numbers of pigs born apart from the first litter in which L(e) sows produced significantly larger litters at birth. The higher energy intakes in pregnancy and lactation led to very similar increases in the weights of the piglets, of the order of 0.1 kg at birth, 0.4 kg at 3 weeks of age, and 1.1 kg at 8 weeks of age. Sowsgiven the same overall energy intake throughout their reproductive life (H(e) m(e), L(e) h(e)) at the end of the third litter had similar live weights and had produced a similar total weight of weaned pigs. The practical implications of these results are discussed.

Ontogeny of the insulin response to glucose in fetal and adult sheep

1989 ◽  
Vol 67 (10) ◽  
pp. 1288-1293 ◽  
Author(s):  
Pamela E. Houghton ◽  
Thomas J. McDonald ◽  
John R. G. Challis
Keyword(s):  
Time Course ◽  
Bolus Injection ◽  
Peak Plasma ◽  
Femoral Vein ◽  
Insulin Response ◽  
Similar Time ◽  
Fetal Sheep ◽  
Adult Sheep ◽  
Glucose Ratio ◽  
Insulin Response To Glucose

The purpose of the present experiments was to examine in sheep whether the fetal insulin response to glucose was present by day 110 (d110) of pregnancy and whether the magnitude of the fetal insulin response changed between d110 and d145 (term). We also compared the responses observed in fetuses to those of adult nonpregnant sheep. Basal concentrations of glucose measured in plasma collected from the fetal femoral artery rose progressively between d110 and d145 of gestation, but did not attain the plasma glucose concentrations measured in adult sheep. Peak glucose concentrations in fetuses were achieved 10 min following the bolus injection of glucose (0.8 g/kg estimated fetal body weight) into the fetal femoral vein, and peak values increased with gestational age. Significantly higher peak glucose concentrations were achieved in adult sheep. The concentration of insulin rose rapidly in fetuses at d110, and a similar time course of insulin release in plasma was seen at all gestational ages. The peak plasma insulin concentrations were achieved at 20 min and were significantly greater in older (d140–145) than younger (d125–130) fetuses (p < 0.05). Peak insulin values in fetuses were much less than in adult sheep. In adult sheep glucose and insulin concentrations remained elevated at 120 min following the injection of glucose, whereas in the fetus the concentration of insulin had returned to preinjection values by 60 min. The insulin/glucose ratio did not change in fetal lambs over the last one third of gestation and was not different from the adult sheep. We conclude that (1) the fetal insulin response to an acute glucose load is present by d110 of gestation, and (2) the ratio of insulin released per unit glucose elevation did not change in fetal sheep over the last one third of gestation, nor between fetal and adult sheep.Key words: glucose, insulin, fetal sheep.

scholarly journals Fat mobilization in Pietrain and Large White pigs

1977 ◽  
Vol 37 (2) ◽  
pp. 167-186 ◽  
Author(s):  
J. D. Wood ◽  
N. G. Gregory ◽  
G. M. Hall ◽  
D. Lister
Keyword(s):  
Body Weight ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Subcutaneous Fat ◽  
Entry Rate ◽  
Large White ◽  
Insulin Metabolism ◽  
Fat Mobilization ◽  
Body Tissues ◽  
Norepinephrine Infusion

1. Two experiments were done with Pietrain and Large White pigs (about 50 kg body-weight) to determine whether fat mobilization is enhanced in the stress-sensitive Pietrains (which also produce pale, soft, exudative (PSE) meat).2. In Expt 1, plasma glucose and insulin concentrations and free fatty acid (FFA) concentrations and composition were measured in five Pietrains and five Large Whites after feeding, during an infusion of norepinephrine (2.5 μg/kg body-weight per min), 16 and 21 h after the withdrawal of food and following insulin administration (0.3 IU/kg body-weight). The entry rate of oleic acid was measured 4 h after feeding. Body composition and longissimus dorsi pH measurements were made on killing the pigs 4 d after the experiment.3. After feeding, the Pietrains tended to have lower concentrations of glucose and insulin in plasma compared with the Large Whites, and higher concentrations of FFA. The Pietrains also had a faster entry rate of oleic acid into body tissues. In fasting (16 h) these differences in hormone and metabolite concentrations were also present and relationships between them and body development could be detected. Pietrains had higher concentrations of FFA and lower concentrations of insulin than Large Whites at a particular stage of the development of fat and muscle (subcutaneous fat weight÷longissimus muscle weight).4. The fatty acid composition of FFA resembled that of the backfat triglycerides in fasting and during norepinephrine infusion but not in the fed state. In particular the proportion of fatty acid 18:1 was low in the fed pigs and that of 18:2 was high. The contrast in FFA composition between the fed and stimulated state was greater in the Large Whites.5. The Pietrains were less sensitive to the antilipolytic action of insulin. Glucose and FFA concentrations were similar in both breeds during the norepinephrine infusion although insulin concentrations were increased to a larger extent in the Large Whites at the termination of the infusion.6. In Expt 2, anaesthetized Pietrain and Large White pigs were given constant infusions of norepinephrine alone (2.5 μg/kg body-weight per min) and norepinephrine+propranolol (2 and 10 μg/kg body-weight per min) or phentolamine (2 and 10 μg/kg body-weight per min). The aim was to determine whether the breeds differed in their sensitivity to norepinephrine when conscious responses to the hormone were prevented.7. When norepinephrine alone was infused, fat mobilization was greater in the Pietrain pigs and glucose concentrations were greater in the Large Whites. Propranolol markedly reduced the lipolytic action of norepinephrine, particularly in the Pietrain pigs studied, and phentolamine reduced its glycogenolytic action, particularly in the Large White pig.8. It seems that the leanness of stress-sensitive, PSE-susceptible Pietrain pigs may be due to an enhanced fat mobilization under various conditions, associated with an impairment in insulin metabolism and a greater sensitivity to the β-adrenergic action of catecholamines on body fat stores.

 -Cell Protein Kinases and the Dynamics of the Insulin Response to Glucose

Diabetes ◽  
2002 ◽  
Vol 51 (Supplement 1) ◽  
pp. S68-S73 ◽  
Author(s):  
R. Nesher ◽  
E. Anteby ◽  
M. Yedovizky ◽  
N. Warwar ◽  
N. Kaiser ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Insulin Response ◽  
Cell Protein ◽  
Insulin Response To Glucose

METABOLISM OF [2-14C] PYRUVATE IN NORMAL, ACROMEGALIC AND HGH-TREATED HUMAN SUBJECTS

1970 ◽  
Vol 65 (1) ◽  
pp. 155-169 ◽  
Author(s):  
W. W. Shreeve ◽  
E. Cerasi ◽  
R. Luft
Keyword(s):  
Glucose Tolerance ◽  
Marked Reduction ◽  
Human Subjects ◽  
Insulin Response ◽  
Human Growth ◽  
Pyruvate Oxidation ◽  
High Insulin ◽  
Low Glucose ◽  
Insulin Response To Glucose ◽  
The Mean

ABSTRACT In 4 studies on 3 acromegalic patients, who had normal iv glucose tolerance and high insulin response to infused glucose (Al), the oxidation to 14CO2 of [2-14C] pyruvate (injected intravenously in trace amount after overnight fast) was not different from that in 9 studies of 9 nonacromegalic »high insulin responders« (Ni). In 4 studies on 3 other acromegalic patients, who had low glucose tolerance and less insulin response to glucose (A2), the formation of 14CO2 was reduced to ½–⅔ that of Al or N1 and was about proportionate to the reduction in glucose tolerance. In A2 the 14CO2 formation was slightly lower than the mean for 10 studies with 7 non-acromegalic subjects, who were »low insulin responders« with normal or low glucose tolerance (N2). Among non-acromegalics expiration of 14CO2 was significantly lower in N2 than in N1. Among 4 non-acromegalic subjects treated with human growth hormone for 3–4 days one had a marked reduction in pyruvate oxidation, while all had a decrease in glucose tolerance. Analysis of 14C in blood glucose at 60 minutes after injection of [2-14C]pyruvate suggested that slightly more total 14C-glucose was present in A2 than N1 without any differences between A2 and N2 or N1 and N2. Two out of 4 studies in A1 showed lower than normal amounts of 14Cglucose. No change in 14C-glucose occurred after administration of HGH. The findings suggest that impairment of pyruvate oxidation accompanies a lowered glucose tolerance in acromegalics with a diabetic tendency. Changes in gluconeogenesis from pyruvate appear to be minimal.

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