A DdeI PCR-RFLP detecting genetic variation of goat POU1F1 gene

2007 ◽  
Vol 87 (1) ◽  
pp. 13-14 ◽  
Author(s):  
X. Y. Lan ◽  
C. Y. Pan ◽  
H. Chen ◽  
C. Z. Lei
Keyword(s):  
Genetic Variation ◽  
Gene Polymorphism ◽  
Key Words ◽  
Milk Yield ◽  
Dairy Goat ◽  
Pou1f1 Gene ◽  
Pcr Rflp ◽  
Silent Allele

We described a DdeI PCR-RFLP method for detecting silent allele at goat POU1F1 locus: p.S241S. Frequencies of D1 allele varied from 0.600 to 1.000 in eight Chinese native breeds. Association of DdeI RFLP genotypes with milk yield of dairy goat was significant (P < 0.05). Key words: Goat, POU1F1 gene, polymorphism, association, milk yield

scholarly journals PSXII-34 The evaluation of the influence of FMO3 gene polymorphism on the milk productivity of Ayrshire breed cows

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 249-250
Author(s):  
Elena Gladyr` ◽  
Elena Konovalova ◽  
Nikolay Sivkin ◽  
Olga V Kostyunina
Keyword(s):  
Gene Polymorphism ◽  
Milk Yield ◽  
Milk Protein ◽  
Cattle Breed ◽  
Monogenic Disease ◽  
World Population ◽  
Milk Productivity ◽  
Pcr Rflp ◽  
Ayrshire Breed ◽  
Significant Superiority

Abstract Russian Ayrshire population of cattle is the second largest in number the world population of animals of this breed. Trimethylaminuria (TMA) is the monogenic disease manifesting in the ‘‘fish off-flavor” of the milk from the sick cows and caused by R238X mutation in the flavin-containing monooxidase 3 gene (FMO3). The aim of our investigation was the analysis of the FMO3 gene polymorphism of Ayrshire cattle breed and its relationship with the milk productivity of the first lactation. The analysis of FMO3 gene polymorphism has been carried out on 352 animals of 5 herds, and associations with the milk productivity due 305 days have been evaluated on 20 healthy cows (RR) and 6 ones-carriers of the mutation (RX). DNA has been extracted by means of the kit “DNA-Extran” (“Syntol”, Russia). The identification of the FMO3 genotypes has been conducted by PCR-RFLP in according to the previously developed method with using TaqI restriction endonuclease. The frequency of animals-carriers of X allele associated with the defect of “fish off-flavor” of milk was in the range 10.21–12.82%. The frequencies of RR, RX and XX genotypes were 76.9, 20.5 and 2.6 %, respectively. The study of relationship between the genotypes on FMO3 gene and productivity traits has shown that the homozygous RR-individuals in comparing to RX-ones had significantly higher levels of the milk yield (+918.6 kg, P ≥ 0.05) and content of the milk protein (+28.8, P ≥ 0.05). Adaptive abilities of RR-cows of Russian selection allowed them to surpass animals of Finnish origin with a similar genotype in milk yield 5,655.6 ± 254.2 against 5,105.0 ± 238.6 kg. The investigations has shown the significant superiority the animals-non carriers of the “fish off-flavor” defect in the milk yield and content of the milk protein. This research was supported by the Ministry of science and higher education АААА-А18-118021590138-1.

scholarly journals A novel EcoRII PCR-RFLP detecting genetic variation of goat <i>NF1-C2</i> gene and its association with milk yield (Brief Report)

2011 ◽  
Vol 54 (2) ◽  
pp. 224-226
Author(s):  
P. Wang ◽  
Q. Xu ◽  
X. Lan ◽  
Z. Li ◽  
M. Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Genetic Variation ◽  
Mammary Gland ◽  
Milk Yield ◽  
Molecular Genetic ◽  
Model System ◽  
Production Traits ◽  
Similar Function ◽  
Milk Production Traits ◽  
Pcr Rflp

Abstract. The transcription factor nuclear factor1-C2 (NF1-C2) mediates the action of prolactin in the mammary gland. Research on the molecular genetic mechanism of model system have indicated that the NF1-C2 gene plays an important role for the activation of several mammary gland specific genes (Nilsson et al. 2006). Moreover, the effects of prolactin on milk production traits have been reported in ruminant (Seriwatanachai et al. 2008). NF1-C2 may have a similar function in goat. To our knowledge polymorphisms of NF1-C2 gene have not been described in animals. Here a SNP of the caprine NF1-C2 was detected and a EcoRII PCR-RFLP was derived, that allowed to clearly detect NF1-C2 genotypes. Further investigation was conduct to evaluate the association between polymorphisms and milk yield at different lactation stages.

​POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat

2021 ◽  
Author(s):  
Vikrant D. Pawar ◽  
Mahadeo P. Sawane ◽  
Aakash Y. Doiphode
Keyword(s):  
Body Weight ◽  
Gene Polymorphism ◽  
Age Groups ◽  
Restriction Enzymes ◽  
Nucleotide Position ◽  
Pou1f1 Gene ◽  
Body Weights ◽  
Pcr Rflp ◽  
Indigenous Goat ◽  
Selection Of

Background: Osmanabadi is the premium meat type goat breed of Maharashtra, known for its adaptability and reproductive efficiency. Identification of superior germplasm and incorporating them into breeding programme is the need of hour for augmenting productivity of Osmanabadi as well as other indigenous goat breeds. Hence, the present research was aimed to study Pituitary Specific Transcription Factor I (POU1F1) gene polymorphism and its associations with body weight of Osmanabadi goat. Methods: Genomic DNA was extracted from 217 blood samples of randomly selected Osmanabadi goats. The 450 bp fragment of POU1F1 gene encompassing exon-6 and its flanking region (3’UTR) was PCR amplified. The PCR amplicon was subjected to RFLP using AluI and PstI restriction enzymes to identify polymorphism at nucleotide position 174 (T greater than C) in exon 6 and at nucleotide position 110 (T greater than C) in the 3’UTR of POU1F1 gene, respectively. The association of the observed allelic variants / genotypes with body weight of the animals was performed using One-way ANOVA. Result: The AluI PCR-RFLP of 450 bp amplicon of POU1F1 gene in Osmanabadi goats revealed polymorphism with three different genotypes viz., ‘CC’, ‘TT’ and ‘TC’. The observed frequencies for TT, TC and CC genotypes were 0.604, 0.355 and 0.041 respectively. The frequencies of T and C alleles were found to be 0.781 and 0.219, respectively. However, PstI PCR-RFLP revealed monomorphic 3’UTR of POU1F1 gene with single TT genotype. We observed significant differences (P less than 0.01) between various genotypes at AluI exon-6 locus of POU1F1 gene and body weights at birth, 3 and 6 months of Osmanabadi goat. Highest body weight was recorded for TT genotype followed by TC and CC genotypes in all three age groups. Our findings indicate that the AluI PCR-RFLP locus of the exon-6 of POU1F1 gene can be used as a potent molecular marker for selection of superior stock of Osmanabadi goats.

scholarly journals Associations between genetic variants of the <i>POU1F1</i> gene and production traits in Saanen goats

2019 ◽  
Vol 62 (1) ◽  
pp. 249-255 ◽  
Author(s):  
Raziye Işık ◽  
Güldehen Bilgen
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Milk Yield ◽  
Restriction Enzymes ◽  
Production Traits ◽  
Pou1f1 Gene ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Flanking Region ◽  
Two Populations

Abstract. This study was conducted to determine the polymorphisms of the POU1F1 gene and their relationships with milk yield and components, litter size, birth weight, and weaning weight in goats. For this purpose, a total of 108 Saanen goats from two different farms (Bornova and Manisa) were used as animal materials. Polymorphisms at the exon 6 and the 3′ flanking region of the POU1F1 gene were determined by using PCR-RFLP with PstI and AluI restriction enzymes and DNA sequencing analyses. Two alleles and three genotypes were identified by AluI or PstI digestions of the POU1F1 gene. The genotypes frequencies of TT, TC, and CC were 64.8 %, 31.5 % and 3.7 % for the PstI locus; 54.6 %, 31.5 % and 13.9 % for the AluI locus, respectively. T allele frequencies (0.56 and 0.88 for the AluI locus, 0.80 and 0.81 for the PstI locus, respectively) were predominant in both loci at the Bornova and Manisa farms. In terms of POU1F1-AluI and POU1F1-PstI loci, two populations were found to be in Hardy–Weinberg equilibrium. In the POU1F1-AluI locus, significant associations were found between genotypes and lactation milk yield and litter size. Similarly, a significant relationship between genotypes and birth weight in the POU1F1-PstI locus (p<0.05) was determined. The TC and CC genotypes were observed to be higher than the TT genotype for lactation milk yield and litter size at the POU1F1-AluI locus. Birth weight was found to be higher in animals that have the CC genotype at the POU1F1-PstI locus. In conclusion, the POU1F1 gene can be used as a molecular marker for economic features like reproduction, growth, milk content and yield in Saanen goats.

scholarly journals PSX-3 Antioxidant system characteristics in Saanen goats depending on performance

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 461-462
Author(s):  
Polina D Bokhan ◽  
Alesya A Bakhta ◽  
Larisa Y Karpenko ◽  
Anna I Kozitcyna ◽  
Anna B Balykina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Milk Yield ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Dairy Goat ◽  
Blood Levels ◽  
Lactation Performance ◽  
Radical Oxidation ◽  
System Characteristics

Abstract Dairy goat breeding is widespread worldwide. Goat milk and other derivative products are an important part of nutrition. Dairy products are the most important part of goat breeding; therefore, lactation performance is a relevant issue. The purpose of this study was to evaluate antioxidant system characteristics in Saanen goats depending on lactation performance. The experiment was conducted in the north-western region of Russian Federation, in the laboratories of the biochemistry and physiology departments at FSBEI of Higher Education «SPbSAVM». The three experimental groups included 30 Saanen goats each, 2nd–3rd lactations, selected using matched pairs method. 1st group included low milk producing ability goats (&lt; 600 kg of milk yield per year), 2nd group included average milk producing ability goats (600–800 kg of milk yield per year), 3rd group included high milk producing ability goats (&gt;800 kg of milk yield per year). The blood samples were taken once: at peak lactation performance (45 days after parturition). The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P &lt; 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics, we found a high intensity of free-radical oxidation in high milk producing ability goats. This results in oxidative stress development. Activity of anti-oxidizing enzymes was elevated; therefore, free-radical oxidation is intensive. Consequently, these data allow us to take into consideration exogenous anti-oxidizing agents administration in high milk producing ability goats in order to reduce oxidative stress.

Reasons for keeping dairy goats in Tanzania, and possible goals for a sustainable breeding program

2017 ◽  
Vol 57 (2) ◽  
pp. 338 ◽  
Author(s):  
Z. C. Nziku ◽  
G. C. Kifaro ◽  
L. O. Eik ◽  
T. Steine ◽  
T. Ådnøy
Keyword(s):  
Milk Yield ◽  
Production Systems ◽  
Holistic Approach ◽  
Breeding Program ◽  
Dairy Goat ◽  
Dairy Goats ◽  
Quantitative And Qualitative Research ◽  
Animal Identification ◽  
Breeding Goals ◽  
And Performance

This research aimed at describing reasons for keeping dairy goats in Tanzania, and possible goals for a sustainable breeding program. Three districts, each representing a unique dairy goat breed population, were selected for the study. The Saanen, Toggenburg and Norwegian were the main dairy goat breeds in Arumeru, Babati, and Mvomero districts, respectively. A total of 125 dairy goat farmers were interviewed. A holistic approach of both quantitative and qualitative research methods was used to study the perceptions of farmers. More milk yield, sale of breeding stock and manure were the highest ranked reasons for keeping dairy goats. The reasons were coherent to the production systems. The three most preferred traits for improvement were milk yield, adaptability and twinning ability. These preferences were absolutely important in the context of the referred production system. Selection of replacement stock, animal identification and performance recording were the main challenges emphasised by farmers. The present study views these challenges as a result of knowledge gaps in animal breeding that require solutions. Based on result findings it is suggested that the milk yield and survival traits should be the primary dairy goat breeding goals. Generally, there are possibilities for developing sustainable dairy goat breeding programs in the surveyed areas given relevant breeding goals are incorporated. The design of simple and manageable dairy goat breeding schemes is necessary.

scholarly journals DAY-TO-DAY VARIATIONS IN FAT PERCENT OF COW'S MILK

1990 ◽  
Vol 70 (2) ◽  
pp. 731-734 ◽  
Author(s):  
A. S. ATWAL ◽  
J. D. ERFLE
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Milk Yield ◽  
Milk Fat ◽  
Rumen Fluid ◽  
Supplemental Feeding ◽  
Fat Percentage ◽  
Milk Fat Depression ◽  
Daily Milk Yield ◽  
Daily Milk

Large day-to-day variations in milk fat, particularly for the morning milkings, were observed in 36 Holstein cows. Changes in percent fat were gradual and produced wavelike patterns in a number of instances. Supplemental feeding of long hay had no effect on acetate/propionate ratio in rumen fluid, daily milk yield or weighted milk fat percentage. Key words: Dairy cows, milk, fat depression, hay

scholarly journals Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat

2002 ◽  
Vol 27 (6) ◽  
pp. 639-643 ◽  
Author(s):  
RITA C. B. WEIKERT-OLIVEIRA ◽  
M. APARECIDA DE RESENDE ◽  
HENRIQUE M. VALÉRIO ◽  
RACHEL B. CALIGIORNE ◽  
EDILSON PAIVA
Keyword(s):  
Triticum Aestivum ◽  
Genetic Variation ◽  
Climatic Factors ◽  
Rapd Analysis ◽  
Its Region ◽  
Restriction Enzymes ◽  
Fungal Species ◽  
High Similarity ◽  
Pcr Rflp ◽  
Upgma Phenogram

Twenty isolates of four fungal species, agents of "Helminthosporium" diseases in cereals, were collected from different regions: nine Bipolarisoryzae isolated from rice (Oryza sativa), seven B.sorokiniana from wheat (Triticum aestivum), two B. maydis, and two Exserohilumturcicum from maize (Zea mays). The strains were compared by PCR-RFLP and RAPD analysis. Size polymorphism among the isolates in the ITS region comprising the 5.8 S rDNA indicated genetic differences among the isolates, while a UPGMA phenogram constructed after the digestion of this region with restriction enzymes showed inter- and intra-specific polymorphism. The RAPD profiles indicated an expressive level of polymorphism among different species, compared with a low level of polymorphism among isolates of the same species. A UPGMA phenogram grouped the isolates according to the species and their host plant. RAPD profiles did not reveal polymorphism that directly correlated climatic factors with geographic source of the isolates of B. sorokiniana, and B. oryzae. Teleomorphic species revealed high similarity with their correspondent anamorphs.

scholarly journals The efficacy of diuretics in complex treatment of patients with hypertension according to the Gly460Trp polymorphism of the α-adducin gene

2021 ◽  
Vol 23 (4) ◽  
pp. 503-508
Author(s):  
S. A. Yermolenko ◽  
V. F. Orlovskyi ◽  
O. V. Orlovskyi ◽  
A. V. Zharkova ◽  
I. O. Moiseienko ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Gene Polymorphism ◽  
Polymorphic Locus ◽  
Polymorphic Marker ◽  
Individual Treatment ◽  
Healthy Individuals ◽  
Group I ◽  
Allelic Distribution ◽  
Pcr Rflp ◽  
Polymerase Chain

The aim of the study was to investigate the effect of thiazide diuretics on blood pressure (BP) depending on Gly460Trp ADD1 gene polymorphism in arterial hypertension (AH) patients of the Ukrainian population in order to predict their individual treatment efficacy. Material and methods. The study included 232 persons: 120 patients with verified stage II AH and 112 healthy individuals. Restriction fragment length polymerase chain reaction (PCR-RFLP) was used to detect genotype (the Gly460Trp-polymorphic locus of the ADD1 gene). The patients received standard therapy, which included ACE inhibitor – ramipril 5 mg, calcium channel antagonist – amlodipine 5 mg, statin – atorvastatin 20 mg, acetylsalicylic acid 75 mg. The patients were randomized into two groups: group I (60 persons) additionally taking treatment with 1.5 mg of indapamide retard and group II (60 persons) – with 25 mg of hydrochlorothiazide. The dynamic reduction of blood pressure has been assessed every 4 weeks for 2 months. Results. Among 120 patients with AH, 91 persons (75.8 %) were homozygous for the G allele (GG), 26 persons (21.7 %) – heterozygous (GT) and 3 persons (2.5 %) – homozygous for the T allele (TT), while the G allele frequency in patients with hypertension was 0.87, and the T allele – 0.13. 98 healthy individuals (87.5 %) were homozygous for the G allele, 13 individuals (11.6 %) were heterozygous, and 1 person (0.9 %) was homozygous for the T allele. The carrier frequency of the G and T alleles was 0.93 and 0.07, respectively. Allelic distribution indicated the predominance of the G allele carriers by Gly460Trp polymorphism of the ADD1 gene among the Ukrainian population, regardless of whether AH symptoms were present. It is noteworthy that the number of the T allele carriers was 2 times large among symptomatic patients than that among healthy individuals. In patients with the T allele, the hypotensive efficacy of indapamide was almost 3 times higher than that in patients with the G allele. The antihypertensive effect of hydrochlorothiazide in patients with the GT and TT genotypes was 2 times greater than that in the GG genotype carriers depending on the presence of the T allele G460T polymorphism of ADD1 gene in the genotype. Conclusions. Allelic distribution indicates the predominance of the G allele carriers by Gly460Trp ADD1 gene polymorphism among the Ukrainian population, regardless of whether AH symptoms are present. Among patients with AH, the accumulation of the T allele G460T polymorphic marker of the α-adducin gene is 2 times more than that in healthy individuals. Patients carrying the T allele demonstrate 2 times higher hypotensive efficacy of indapamide compared with hydrochlorothiazide.

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