Influence of canola oil on the fatty acid profile in goats’ milk

2003 ◽  
Vol 83 (2) ◽  
pp. 323-325 ◽  
Author(s):  
E. K. Okine ◽  
L. A. Goonewardene ◽  
Z. Mir ◽  
P. Mir ◽  
Z. Wang ◽  
...  

Four Alpine does were used in a 4 × 4 Latin square design to determine the effects of feeding canola oil at four levels: 0, 2, 4 and 6% on milk fatty acid profile. The sum of C12:0 + C14:0 + C16:0 (hypercholesterolemic fatty acids) decreased linearly and C18:0 + C18:1: C16:0 (indicator of cholesterolemic tendency of fat source) increased linearly (P < 0.01) with increased canola oil intake. Key words: Goat, milk, fatty acids, canola oil

2012 ◽  
Vol 13 (4) ◽  
pp. 1180-1196
Author(s):  
Jefferson Rodrigues Gandra ◽  
Francisco Palma Rennó ◽  
José Esler Freitas Júnior ◽  
Milton Maturana Filho ◽  
Rafael Villela Barletta

The aim of this study was to evaluate the nutrients balance and milk fatty acids profile of dairy cows supplemented with monensin. Twelve Brazilian Holstein dairy cows were distributed into four balanced 3x3 Latin squares, and fed with the following diets: control (C), basal diet without addition of monensin, monensin 24 (M24), addition of 24mg/kg DM of monensin, and monensin 48 (M48), addition of 48mg/kg DM. The experimental diets influenced the efficiency of net energy of lactation utilization. A quadratic effect was observed for the energy balance. It was observed effect of diets on nitrogen balance. It was observed effect of monensin in the milk yield, composition and in the milk fatty acids profile. Monensin in diets of dairy cows in mid lactation, using corn silage, improved the nutrients balance and milk fatty acid profile with 24mg/kg DM.


Author(s):  
Klára Novotná ◽  
Milena Fantová ◽  
Lenka Nohejlová ◽  
Markéta Borková ◽  
Luděk Stádník ◽  
...  

The aim of this study was to investigate the effect of two species of the microalgae on the milk yield, the basic composition and the fatty acid profile of goat milk, with focus on n‑3 fatty acids. Forty‑five White short‑haired goats were randomly allocated to three groups; the control group (C) with no supplementation microalgae to the diet. The first experimental group (Ch) was supplemented with Chlorella vulgaris and second experimental group (J) has been supplemented with Japonochytrium sp. The Japonochytrium supplementation negatively affected milk yield, but the amount of milk fat (+0.1 %; +0.45 %) and solids‑not‑fat (+0.27 %; +0.86 %) were higher than in group C and Ch. The amount of polyunsaturated (5.527 % ± 0.378) and saturated (71.560 % ± 0.861) fatty acids was also highest in group J. An increase of C20:4, C20:5 was detected in J and Ch, and the concentration of C22:6 was highest in group J (+0.019 %; P < 0.001).


2011 ◽  
Vol 51 (2) ◽  
pp. 95 ◽  
Author(s):  
O. Dayani ◽  
G. R. Ghorbani ◽  
A. K. Esmailizadeh

Eight multiparous Holstein cows in mid lactation (average days in milking of 160 ± 40) were used in a replicated 4 by 4 Latin square design, each experimental period lasting 3 weeks, to determine the effects of whole cottonseed (WCS) treatment and dietary crude protein (CP) concentration on the profile of milk fatty acids. Each 3-week experimental period consisted of 2 weeks for ration adaptation followed by 1 week for data collection. The experimental diets consisted of: (1) Control (without WCS), 16% CP; (2) 20% WCS, 16% CP; (3) 20% WCS, 13% CP; and (4) 20% crushed WCS, 13% CP. Minerals and vitamin supplements were the same in all experimental diets. The WCS treatment led to increased DM intake, fat-corrected milk yield, fat concentration, proportion of long-chain fatty acids, unsaturated fatty acids and ratio of unsaturated to saturated fatty acids (P < 0.05). Decreased concentration of dietary CP from 16 to 13% (diet 2 compared with diet 3) led to a decrease in both milk yield and milk composition (P < 0.05). WCS crushed in diets with 13% CP (diet 4 compared with diet 3) increased (P < 0.05) the concentration of conjugated linoleic acid in milk fat. The results demonstrated that adding WCS to the diet of lactating cows improves both milk yield and milk fatty acid profile, particularly the proportion of conjugated linoleic acid.


2017 ◽  
Vol 84 (2) ◽  
pp. 170-180 ◽  
Author(s):  
Gianfranco Cosenza ◽  
Nicolò P P Macciotta ◽  
Anna Nudda ◽  
Angelo Coletta ◽  
Luigi Ramunno ◽  
...  

The oxytocin receptor, also known as OXTR, is a protein which functions as receptor for the hormone and neurotransmitter oxytocin and the complex oxytocin–oxytocin receptor plays an important role in the uterus during calving. A characterisation of the river buffalo OXTR gene, amino acid sequences and phylogenetic analysis is presented. The DNA regions of the OXTR gene spanning exons 1, 2 and 3 of ten Mediterranean river buffalo DNA samples were analysed and 7 single nucleotide polymorphisms were found. We focused on the g.129C > T SNP detected in exon 3 and responsible for the amino acid replacement CGCArg > TGCCys in position 353. The relative frequency of T allele was of 0·257. An association study between this detected polymorphism and milk fatty acids composition in Italian Mediterranean river buffalo was carried out. The fatty acid composition traits, fatty acid classes and fat percentage of 306 individual milk samples were determined. Associations between OXTR g.129C > T genotype and milk fatty acids composition were tested using a mixed linear model. The OXTR CC genotype was found significantly associated with higher contents of odd branched-chain fatty acids (OBCFA) (P < 0·0006), polyunsaturated FA (PUFA n 3 and n 6) (P < 0·0032 and P < 0·0006, respectively), stearic acid (C18) (P < 0·02) and lower level of palmitic acid (C16) (P < 0·02). The results of this study suggest that the OXTR CC animals might be useful in selection toward the improvement of milk fatty acid composition.


animal ◽  
2016 ◽  
Vol 10 (8) ◽  
pp. 1303-1310 ◽  
Author(s):  
J.R. Gandra ◽  
R.D. Mingoti ◽  
R.V. Barletta ◽  
C.S. Takiya ◽  
L.C. Verdurico ◽  
...  

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 174-175
Author(s):  
Jan C Plaizier ◽  
Sharon Y Mowete ◽  
Debora Santchi ◽  
Ken Kwiatkowski ◽  
Nympha De Neve ◽  
...  

Abstract The accuracy of the milk fatty acid profile as a diagnostic tool for the diagnosis of sub-acute ruminal acidosis (SARA) has been determined when SARA was experimentally induced. This had not yet been done not on commercial dairy farms, where SARA can occur naturally. The objective of this study was to determine this accuracy in individual cows on commercial dairy farms. A total of 336 cows from 24 commercial dairy farms in Quebec were included. Farms were blocked based on geographical location and management, with each block having one high risk SARA farm and one low risk SARA farm. Farm Risk of SARA was determined based on the milk fat content and the proportions of de novo fatty acids and long chain unsaturated fatty acids in the bulk tank. On each farm, 7 early/mid-lactation (&lt; 150 days in milk DIM) and 7 mid/late lactation (&lt; 150 DIM) cows were randomly selected. The fatty acid profile of pooled milk samples from these cows were determined by gas chromatography. Farm risk of SARA did not affect the milk fat proportion of fatty acids, with the exception of trans 10 cis 12 C18:2, which was higher in At Risk Farms. Later lactation cows had a higher milk fat content and higher milk fat proportions of de novo, C16 fatty, and odd and branch chain fatty acids. The prevalence of SARA was likely higher in earlier lactation cows than in later lactation cows, but non-SARA related animal and dietary factors also affect the milk fatty acid profile. Hence, the milk fatty acid profile alone may not be accurate enough to diagnose SARA on farm. This profile can, however, contribute to this diagnosis, the identification of causes of milk fat depression, and the development of strategies to optimize the milk fatty acid profile.


1995 ◽  
Vol 75 (2) ◽  
pp. 267-269 ◽  
Author(s):  
E. J. DePeters ◽  
J. F. Medrano ◽  
B. A. Reed

The proportion of MCFA in milk fat was lowest for Holstein, highest for Jersey, and intermediate for Brown Swiss cows. Proportions of SCFA (C4:0 to C8:0) and LCFA (> 18 C) did not differ among breeds. Differences in fatty acid composition among breeds were small, but could contribute to differences in manufacturing properties of milk fat. Key words: Holstein, Jersey, Brown Swiss, milk fatty acids


2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3629
Author(s):  
Regis Luis Missio ◽  
Luis Fernando Glasenapp de Menezes ◽  
Ivan Luiz Brondani ◽  
José Laerte Nörnberg ◽  
Paulo Santana Pacheco ◽  
...  

The objective of this study was to evaluate the fatty acid profile of duodenal digesta (experiment I) and of meat of beef cattle (experiment II) fed diets containing different levels of concentrate (220, 400, 590 and 790 g of concentrate/kg of dry matter of the diets). The experiment I was conducted with four Charolais-Nellore steers (460 ± 18.2 kg of BW), with a T-shaped duodenal cannula, using a double 4 × 4 Latin square as an experimental design. In experiment II, 16 crossbred Charolais-Nellore young bulls (192.44 ± 18.2 kg of BW) were randomly distributed in the experimental treatments (220, 400, 590 and 790 g of concentrate/kg of dry matter of the diets). The diets were isonitrogenous (120 g of crude protein/kg of dry matter). The intramuscular fat content was used as a covariant for the statistical analysis of the meat fatty acid profile. The duodenal content of fatty acid C17:0 decreased with increase of concentrate levels, while its content in the meat presented a quadratic variation with the increase of the concentrate levels of the diets, being the lowest values observed for the diet with 400 g of concentrate. The duodenal content of fatty acid C18:1 trans-11 decreased, whereas the content of this fatty acid in the meat increased with the increase of the dietary concentrate levels. The increase in the level of concentrate reduced the content of polyunsaturated fatty acids C18:3 n-3, C20:3 n-6, C20:4 n-6, and C20:5 n-3 EPA in both the duodenal digesta and meat. No difference was observed in the n-6/n-3 fatty acids ratio (mean of 13.96) of the meat between diets. The elevation of the level of concentrate in confinement diets reduces the nutraceutical quality of the meat of Charolais-Nellore young bulls slaughtered at 14-16 months of age due to the reduction of the polyunsaturated fatty acids content important for human health.


2020 ◽  
Author(s):  
Mingyue Cao ◽  
Lijun Shi ◽  
Peng Peng ◽  
Bo Han ◽  
Lin Liu ◽  
...  

Abstract Background: Our previous genome-wide association study (GWAS) on milk fatty acid traits in Chinese Holstein cows revealed, the SNP, BTB-01556197, was significantly associated with C10:0 at genome-wide level (P = 0.0239). It was located in the down-stream of 5-hydroxytryptamine receptor 1B (HTR1B) gene that has been shown to play an important role in the regulation of fatty acid oxidation. Hence, we considered it as a promising candidate gene for milk fatty acids in dairy cattle. In this study, we aimed to investigate whether the HTR1B gene had significant genetic effects on milk fatty acid traits.Results: We re-sequenced the entire coding region and 3,000 bp of 5' and 3' flanking regions of HTR1B gene. A total of 13 SNPs was identified, containing one in 5' flanking region, two in 5' untranslated region (UTR), two in exon 1, five in 3' UTR, and three in 3' flanking region. By performing genotype-phenotype association analysis with SAS9.2 software, we observed that 13 SNPs were significantly associated with medium-chain saturated fatty acids such as C6:0, C8:0 and C10:0 (P < 0.0001 ~ 0.042). With Haploview 4.1 software, linkage disequilibrium (LD) analysis was performed that two haplotype blocks formed by two and ten SNPs were observed. Haplotype-based association analysis indicated that both haplotype blocks were strongly associated with C6:0, C8:0 and C10:0 as well (P < 0.0001 ~ 0.0071). With regards to the missense mutation in exon 1 (g.17303383G>T) that reduced amino acid change from alanine to serine, we predicted that it altered the secondary structure of HTR1B protein with SOPMA. In addition, we predicted that three SNPs in promoter region, g.17307103A>T, g.17305206T>G and g.17303761C>T, altered the binding sites of transcription factors (TFs) HMX2, PAX2, FOXP1ES, MIZ1, CUX2, DREAM, and PPAR-RXR by Genomatix. Of them, luciferase assay experiment further confirmed that the allele T of g.17307103A>T significantly increased the transcriptional activity of HTR1B gene than allele A (P = 0.0007).Conclusions: In conclusion, our findings provided first evidence that the HTR1B gene had significant genetic effects on milk fatty acids in dairy cattle.


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