The feeding value of grain screenings for ruminants: Chemical composition and nutrient utilization

2000 ◽  
Vol 80 (4) ◽  
pp. 673-680 ◽  
Author(s):  
T. A. Marx ◽  
J. J. McKinnon ◽  
A. F. Mustafa ◽  
D. A. Christensen ◽  
V. J. Racz
Keyword(s):  
Crude Protein ◽  
Nutrient Utilization ◽  
Barley Grain ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Gross Energy ◽  
Feeding Value ◽  
Ruminal Degradability ◽  
Voluntary Intake ◽  
Different Levels

The objectives of this study were to determine the chemical and nutrient utilization characteristics of grain screening pellets (GSP) for ruminants. One ruminally fistulated cow was used to determine ruminal nutrient degradability characteristics of GSP relative to barley grain. Twenty-one growing lambs were used to determine voluntary intake and total tract nutrient digestibility coefficients for GSP relative to regular and thin barley. Seven diets were formulated with different levels of GSP, and thin or regular barley at ratios of 100:0; 75:25; 50:50; 25:75. On a dry matter (DM) basis, GSP contained 337 ± 17.4 g kg−1 neutral detergent fiber (NDF), 209 ± 21.1 g kg−1 acid detergent fiber (ADF), 151 ± 11.1 g kg−1 crude protein (CP), and 262 ± 61.5 g kg−1 starch. Ruminal degradability of DM and NDF of GSP was lower (P < 0.05) while that for CP was higher (P < 0.05) than barley grain. Effective ruminal degradability of starch for GSP and barley exceeded 900 g kg−1. Results of the sheep trial showed that as the level of GSP increased, voluntary intake increased (P < 0.05) and total tract digestibility coefficients of DM, NDF, and gross energy (GE) decreased (P < 0.05). The digestibility coefficients of DM, NDF, and GE for GSP were 666 g kg−1, 294 g kg−1, and 698 J kJ−1, respectively. The digestible energy (DE) content of GSP (13.8 MJ kg−1) was similar to that of thin barley and 11% lower (P < 0.5) than that of regular barley. It was concluded that while GSP exhibited reduced ruminal and total tract nutrient utilization relative to regular barley, this feed product is a good source of energy and protein for growing ruminants. Key words: Grain screening pellets, barley, thin barley, nutrient utilization

scholarly journals Ensiling characteristics, ruminal nutrient degradabilities and whole tract nutrient utilization of berseem clover (Trifolium alexandrinum L.) silage

2003 ◽  
Vol 83 (1) ◽  
pp. 147-152 ◽  
Author(s):  
A. F. Mustafa ◽  
P. Seguin
Keyword(s):  
Crude Protein ◽  
Nutrient Utilization ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Protein Nitrogen ◽  
Gross Energy ◽  
Ruminal Degradability ◽  
Berseem Clover ◽  
Ram Lambs ◽  
True Protein

A study was conducted to determine ensiling characteristics and nutrient utilization by ruminants of berseem clover silage (BCS). Ensiling characteristics were determined by ensiling forage in mini-silos for 0, 2, 4, 8, 16 and 70 d. Two lactating Holstein cows fitted with ruminal cannulas were utilized to determine ruminal nutrient degradabilities while six ram lambs were used to determine total tract nutrient digestibilities. Ruminal and whole tract nutrient utilization of BCS were compared with those of alfalfa silage (AS). Results showed that BCS went through a rapid fermentation as shown by a significant (P < 0.05) reduction in pH during the first 2 d post-ensiling. Most of the proteolytic activity took place between day 0 and day 2 as indicated by a reduction (P < 0.05) in true protein and neutral detergent insoluble protein and an increase (P < 0.05) in non-protein nitrogen. Relative to AS, BCS had a similar ruminal degradability of dry matter (DM), a lower degradability of crude protein (CP), and a higher (P < 0.05) degradability of neutral detergent fiber (NDF). Relative to AS, BCS had a higher (P < 0.05) whole-tract digestibility of DM (70.7 vs. 66.0%), organic matter (71.5 vs. 65.9%), NDF (65.6 vs. 54.5), acid detergent fiber (64.6 vs. 54.6) and gross energy (69.6 vs. 64.0), but a lower (P < 0.05) wholetract digestibility of CP (69.2 vs. 75.9%). These results suggest that BSC could replace AS as a forage source for ruminants. Key words: Berseem clover silage, ruminal degradability, total tract nutrient utilization

scholarly journals Effects of Black Wattle (Acacia mearnsii) Condensed Tannins on Intake, Protozoa Population, Ruminal Fermentation, and Nutrient Digestibility in Jersey Steers

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1011 ◽  
Author(s):  
Andre S. Avila ◽  
Maximiliane A. Zambom ◽  
Andressa Faccenda ◽  
Maria L. Fischer ◽  
Fernando A. Anschau ◽  
...  
Keyword(s):  
Organic Matter ◽  
Condensed Tannins ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acacia Mearnsii ◽  
Black Wattle

The objective of this study was to evaluate the effect of inclusion of condensed tannins (CT) from black wattle (Acacia mearnsii) on feed intake, ruminal protozoa population, ruminal fermentation, and nutrient digestibility in Jersey steers. Five ruminally-cannulated steers were used in a 5 × 5 Latin square design, with five periods of 20 days each (14 days for diet adaptation and six days for sample collection per period). Treatments were composed of dietary inclusion levels of condensed tannins at 0, 5, 10, 15, and 20 g/kg of diet dry matter. Intakes of dry matter, organic matter, ether extract, crude protein, neutral detergent fiber, and total digestible nutrients were not affected by condensed tannins. The ruminal pH was reduced linearly with tannin levels. Ruminal ammonia nitrogen concentration was not affected by tannins. Tannins reduced the molar proportion of acetate and did not affect the ruminal protozoal population, which might be related to the low doses used. Digestibilities of dry matter, organic matter, and neutral detergent fiber were not altered; however, there was a linear reduction in crude protein digestibility. Based on these results, CT extracts from black wattle are not recommended for improving nutrient utilization in steers at the tested levels.

Effects of processing and fat content of coarse canola screenings on voluntary intake and total tract nutrient digestibility of beef steers

2000 ◽  
Vol 80 (1) ◽  
pp. 153-159 ◽  
Author(s):  
S. Pylot ◽  
J. J. McKinnon ◽  
A. F. Mustafa ◽  
V. J. Racz ◽  
D. A. Christensen
Keyword(s):  
Dietary Fat ◽  
Protein Digestibility ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Fat Level ◽  
Voluntary Intake

The effects of feeding canola screenings on voluntary intake (VI) and nutrient digestibility by growing steers were determined in two experiments. Twelve steers were involved in the first experiment to study the effects of processing (grinding and pelleting) of canola screenings on VI and apparent total tract dry matter digestibility (DMD), crude protein digestibility (CPD), fatty acid digestibility (FAD), neutral detergent fiber digestibility (NDFD), and acid detergent fiber digestibility (ADFD). In the second experiment, the effects of fat level from canola screenings on VI and total tract nutrient digestibilities were determined using 12 steers. The fat levels tested were 67, 100, 128, and 162 g kg−1 ether extract (EE). Feeding processed canola screenings (176 g kg−1 EE) reduced (P < 0.05) VI by 31% and improved (P < 0.05) DMD, CPD, and FAD by 18, 19, and 28%, respectively. Processing of canola screenings reduced (P < 0.05) ADFD by 15% with no effect on NDFD. Increasing dietary fat level from 67 to 162 g kg−1 reduced VI in a quadratic manner (P < 0.05) and improved NDFD in a linear (P < 0.05) fashion. However, DMD, CPD, and ADFD were not affected by fat level in the diet. Total tract digestibility of fatty acids decreased linearly (P < 0.05) from 822 to 640 g kg−1 with increasing dietary fat level. The results of the study indicate that dietary fat from canola screenings at levels up to 100 g kg−1 can be fed to growing steers without detrimental effects on feed intake. Key words: Canola screenings, fat, intake, nutrient utilization, steers.

scholarly journals Intake and nutrient digestibility of tropical grasses and legume hay supplemented with tilapia fish silage

1969 ◽  
Vol 92 (1-2) ◽  
pp. 27-38
Author(s):  
Héctor L. Díaz-Ríos ◽  
Abner A. Rodríguez-Carias
Keyword(s):  
Crude Protein ◽  
Latin Square ◽  
Basal Diet ◽  
Protein Digestibility ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Arachis Glabrata ◽  
Fiber Digestibility ◽  
Digitaria Eriantha ◽  
Voluntary Intake

Two experiments were conducted to determine the effect of supplementation with tilapia fish silage (FS) prepared in laboratory scale silos with addition of cane molasses, on intake and digestibility of tropical grass hay (GH) (Digitaria eriantha, 80%, and Urochloa maximum, 20%), and rhizome perennial peanut hay (RPPH) (Arachis glabrata). In Experiment I, GH was the basal diet and treatments were Control or T1 = GH, and GH supplemented daily with FS at 0.45% (T2) and 0.90% (T3) of the animal body weight (BW). In Experiment II, the basal diet was RPPH and treatments were Control or T1 = RPPH;T2 = RPPH supplemented daily with FS at 0.225% BW; and T3 = RPPH with 0.45% BW. In both experiments nine adult intact rams were used. The animals were fed for eight days of adaptation and six days of data collection in each period. Both experiments used a 3 X 3 Latin Square design. In experiment I dry matter (DM) intake and digestibility of GH increased (P less than 0.05) with FS supplementation, which also increased crude protein (CP) intake. CP digestibility increased (P < 0.05) at each level of supplementation compared to that of the control, but T2 and T3 did not differ significantly. Neutral detergent fiber (NDF) intake was higher (P < 0.05) in T1 than in T3 but similar in T1 and T2. Fiber digestibility was not affected (P greater than 0.05) by the treatments. It was concluded that supplementation of a GH basal diet with FS at 0.90% of BW is the best alternative. In Experiment II, DM digestibility of T3 differed (P less than 0.05) from that in T1 but in T2 did not differ from DM digestibility either in T1 or T3. Intake of DM, CP and fiber were similar in all treatments (P greater than 0.05). Crude protein digestibility was similar at both levels of supplementation and superior (P < 0.05) to that of the control, but fiber digestibility was not affected (P > 0.05). It is concluded that daily supplementation with FS for sheep fed a basal diet of RPPH should be limited to 0.225% of BW. Upon comparing both experiments, voluntary intake of DM, NDF and CP from RPPH was higher than from GH.The legume also showed a higher (P < 0.05) digestibility of DM and CP.

scholarly journals PSVIII-1 Determination of in vitro DM, N and NDF digestibility and fermentability in novel corn co-products for swine

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 206-207
Author(s):  
Amanda Palowski ◽  
Zhaohui Yang ◽  
Pedro E Urriola ◽  
Gerald C Shurson
Keyword(s):  
Nutrient Digestibility ◽  
High Protein ◽  
Corn Fiber ◽  
Neutral Detergent Fiber ◽  
Dent Corn ◽  
Gross Energy ◽  
Feeding Value ◽  
Corn Ddgs

Abstract New process technologies are being implemented in the ethanol industry resulting in new high protein corn co-products (HPCP) containing more gross energy and amino acids than conventional distillers dried grains with solubles (DDGS). Thus, estimates of nutrient digestibility for HPCP are needed to assess potential feeding value for swine. Furthermore, yellow dent corn is the predominant grain source in U.S. ethanol and co-product production whereas flint corn is used in Brazil. This study determined and compared in vitro dry matter (DM), nitrogen (N), and neutral detergent fiber (NDF) digestibility and fermentability of U.S. and Brazil corn DDGS and new HPCP. In vitro methods evaluated DM, N, and NDF digestibility and fermentability of 2 corn DDGS samples from U.S. (POET and ICM), 2 DDGS samples from Brazil (BR1 and BR2), 1 corn fiber and solubles sample (CF+S), 3 high protein distillers grains (HP-DDG) samples (BR-HP, US-HP, US-HPG1.5), and 2 HP-DDG samples containing high yeast (Ultramax; UM) and StillPro (SP). Digestibility of DM from hydrolysis varied and was greatest for UM (87.13%; P &lt; 0.001) compared with all other co-products. Fermentability of DM was greater (P &lt; 0.001), and similar for UM (85.6%), SP (76.8%), and US-HP-DDG (72.8%) compared with other co-products. Digestibility of N was greater for UM (91.2%; P &lt; 0.001) compared with all other co-products. NDF digestibility was greater (P &lt; 0.001) for UM (62.5%) and SP (60.5%) compared with all other co-products. POET and ICM DDGS had greater (P &lt; 0.001) DM hydrolysis (78.7% and 68.2%, respectively), than BR1 (37.2%) and BR2 (35.0%) and N digestibility (POET = 65.0%; ICM = 58.9%) compared with BR1 (54.4%) and BR2 (43.4%). These results suggest greater nutritional value for Ultramax and StillPro than conventional DDGS and HP-DDG sources (U.S. and Brazilian) due to greater DM, N, and NDF digestibility.

scholarly journals The influence of xylanase on the fermentability, digestibility, and physiochemical properties of insoluble corn-based fiber along the gastrointestinal tract of growing pigs

2021 ◽  
Author(s):  
Amy L Petry ◽  
Nichole F Huntley ◽  
Michael R Bedford ◽  
John F Patience
Keyword(s):  
Mixed Model ◽  
Linear Mixed Model ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Neutral Detergent Fiber ◽  
Adaptation Period ◽  
High Fiber ◽  
Gross Energy ◽  
Digesta Viscosity ◽  
The Impact

Abstract In theory, supplementing xylanase in corn-based swine diets should improve nutrient and energy digestibility and fiber fermentability, but its efficacy is inconsistent. The experimental objective was to investigate the impact of xylanase on energy and nutrient digestibility, digesta viscosity, and fermentation when pigs are fed a diet high in insoluble fiber (&gt;20% neutral detergent fiber; NDF) and given a 46-d dietary adaptation period. Three replicates of 20 growing gilts were blocked by initial body weight, individually housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF) with 7.5% NDF, a 30% corn bran high-fiber control (HF; 21.9% NDF), HF+100 mg xylanase/kg [HF+XY, (Econase XT 25P; AB Vista, Marlborough, UK)] providing 16,000 birch xylan units/kg; and HF+50 mg arabinoxylan-oligosaccharide (AXOS) product/kg [HF+AX, (XOS 35A; Shandong Longlive Biotechnology, Shandong, China)] providing AXOS with 3-7 degrees of polymerization. Gilts were allowed ad libitum access to fed for 36-d. On d 36, pigs were housed in metabolism crates for a 10-d period, limit fed, and feces were collected. On d 46, pigs were euthanized and ileal, cecal, and colonic digesta were collected. Data were analyzed as a linear mixed model with block and replication as random effects, and treatment as a fixed effect. Compared with LF, HF reduced the apparent ileal digestibility (AID), apparent cecal digestibility (ACED), apparent colonic digestibility (ACOD), and apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), crude protein (CP), acid detergent fiber (ADF), NDF, and hemicellulose (P&lt;0.01). Relative to HF, HF+XY improved the AID of GE, CP, and NDF (P&lt;0.05), and improved the ACED, ACOD, and ATTD of DM, GE, CP, NDF, ADF, and hemicellulose (P&lt;0.05). Among treatments, pigs fed HF had increased hindgut DM disappearance (P=0.031). Relative to HF, HF+XY improved cecal disappearance of DM (162 vs. 98g; P=0.008) and NDF (44 vs. 13g; P&lt;0.01). Pigs fed xylanase had a greater proportion of acetate in cecal digesta and butyrate in colonic digesta among treatments (P&lt;0.05). Compared with LF, HF increased ileal, cecal, and colonic viscosity, but HF+XY decreased ileal viscosity compared with HF (P&lt;0.001). In conclusion, increased insoluble corn-based fiber decreases digestibility, reduces cecal fermentation, and increases digesta viscosity, but supplementing xylanase partially mitigated that effect.

scholarly journals Effects of Defatted Rice Bran Inclusion Level on Nutrient Digestibility and Growth Performance of Different Body Weight Pigs

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1374
Author(s):  
Bingbing Huang ◽  
Huangwei Shi ◽  
Li Wang ◽  
Lu Wang ◽  
Zhiqian Lyu ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Rice Bran ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Neutral Detergent Fiber ◽  
Control Group ◽  
Defatted Rice Bran ◽  
Gross Energy ◽  
Stage 1

This study was conducted to determine the effects of low-protein diet prepared with different levels of defatted rice bran (DFRB) and weight stages on growth performance and nutrient digestibility of growing–finishing pigs. The animal experiment included three stages. A total of 240 growing pigs with an initial body weight of 28.06 ± 8.56 kg for stage 1 were allocated to five diets including one control group and four DFRB diets supplemented with 2.5%, 5%, 7.5% and 10% DFRB, respectively. The 192 crossbred pigs with initial body weights of 55.03 ± 7.31 kg and 74.55 ± 9.10 kg were selected for stage 2 and stage 3, respectively. Pigs were allocated to four diets including one control group and three DFRB diets supplemented with 10%, 15% and 20% DFRB, respectively. The results showed that with the increase in DFEB intake, the gain: feed was linearly increased (p < 0.05), and the average daily feed intake tended to linearly decrease (p = 0.06) in stage 1. Except for the apparent total tract digestibility (ATTD) of acid detergent fiber (ADF) in stage 3, levels of DFRB had significant effects on the ATTD of gross energy (GE), dry matter (DM), ash, neutral detergent fiber (NDF) and ADF in three weight stages. In stage 1, with the increase in levels of DFRB, the ATTD of NDF and hemicellulose were firstly increased and then decreased (p < 0.01). In stage 2, with the increasing levels of DFRB, the ATTD of DM, ash and cellulose were firstly increased and then decreased (p < 0.01). In stage 3, the ATTD of GE, DM, ash, NDF and hemicellulose decreased linearly with the increase in levels of DFRB (p < 0.01). Collectively, DFRB could be used as a replacement for corns and soybean meal, and weight stage is important to consider when adjusting the additive proportion.

scholarly journals Feed Utilisation Efficiency of Lupin Inclusion in Cobia: Role of Dietary Organic Selenium Supplementation

2016 ◽  
Vol 10 (10) ◽  
pp. 180 ◽  
Author(s):  
Hung Duc Pham ◽  
Ravi Fotedar ◽  
Chau Minh Nguyen ◽  
Muhammad Abu Bakar Siddik
Keyword(s):  
Crude Protein ◽  
Nutrient Digestibility ◽  
Organic Selenium ◽  
Gross Energy ◽  
Feed Utilisation ◽  
Kernel Meal ◽  
Flow Through ◽  
Inclusion Level ◽  
Utilisation Efficiency

The dietary selenium (Se) requirement has been determined for cobia Rachycentron canadum using purified diet; however, its role in the utilisation of plant-derived ingredients has not been evaluated in the species. Therefore, a 3 x 2 factorial experimental design involving three inclusion levels (0, 210 and 315 g/kg) of lupin Lupinus angustifolius kernel meal (LKM) and two concentrations (0 and 0.8 mg/kg) of Se was used to evaluate the effects of Se supplementation in conjunction with LKM on the growth, feed utilisation and physiological responses in the cobia. Six isonitrogenous (46.5 % crude protein) and isoenergetic (21 MJ/kg gross energy) diets were formulated and fed to cobia for 7 weeks in a flow-through seawater system. The results showed significant effects of Se supplementation and its interaction with dietary lupin on the growth and feed efficiency of cobia. Se supplementation significantly improved the growth and feed utilisation efficiency in cobia fed lupin-based diets. The nutrient digestibility of fish fed supplemental Se lupin-based diets was significantly higher than that of fish fed diets without Se supplementation at each inclusion level of LKM. There were no significant effects of dietary Se supplementation on the survival, muscle composition or muscle amino acids, whereas regression analysis indicated a positive linear relationship between tissue Se accumulation and dietary Se levels. An enhancement of haematological responses was also observed in cobia fed Se-supplemented diets. In summary, cobia fed lupin-based diets required higher dietary Se supplementation for higher feed utilisation efficiency than previously quantified for the casein-based diet.

scholarly journals Chemical composition and rumen degradability of byproducts available in the Amazon region

2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3605
Author(s):  
Ernestina dos Ribeiro Santos Neta ◽  
Luis Rennan Sampaio Oliveira ◽  
Rafael Mezzomo ◽  
Daiany Íris Gomes ◽  
Janaina Barros Luz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Palm Kernel ◽  
Neutral Detergent Fiber ◽  
Daily Diet ◽  
Pineapple Peel ◽  
Ruminal Degradability ◽  
Babassu Cake

This study evaluated the chemical composition and ruminal degradability of dry matter (DM), neutral detergent fiber corrected for ash and protein (NDFap) and crude protein (CP) in byproducts of African oil palm (palm cake, kernel or fiber), macaúba (pulp cake and kernel cake), acai (acai fruit), babassu (kernel cake) and pineapple (peel, crown and bagasse silage). Nineteen rumen-fistulated sheep were kept in individual stalls, receiving a daily diet composed of elephant grass silage and corn and soybean concentrate. After preparation in nylon bags, the byproduct samples were incubated for 0, 3, 6, 12, 16, 18, 24, 48, 72, 96, 120 and 144 hours, with three replicates of each ingredient per incubation time. The divergence between the protein nutritional value and energy nutritional value, based on discriminatory variables between groups, was estimated by cluster analysis. The effective degradability of DM, NDFap and CP for the different byproducts was, respectively, 35.9, 26.9 and 59.0% for palm cake; 48.3, 34.3 and 76.4% for palm kernel; 21.1, 6.6 and 50.3% for palm fiber; 34.3, 15.0 and 52.8% for macaúba pulp cake; 58.1; 63.0 and 51.6% for macaúba kernel cake; 49.7, 49.6 and 41.8% for babassu cake; 53.4, 40.5 and 79.8% for pineapple bagasse silage; and 21.3, 17.0 and 38.9% for acai fruit. Based on their NDFap and CP characteristics, the feeds were clustered in up to four different groups.

scholarly journals Effects of copper hydroxychloride and distillers dried grains with solubles on intestinal microbial concentration and apparent ileal and total tract digestibility of energy and nutrients by growing pigs1

2019 ◽  
Vol 97 (12) ◽  
pp. 4904-4911 ◽  
Author(s):  
Charmaine D Espinosa ◽  
R Scott Fry ◽  
Matthew E Kocher ◽  
Hans H Stein
Keyword(s):  
Large Intestine ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
Total Concentration ◽  
Nutrient Digestibility ◽  
Ether Extract ◽  
Microbial Protein ◽  
Distillers Dried Grains ◽  
Gross Energy

Abstract An experiment was conducted to test the hypothesis that Cu hydroxychloride improves nutrient digestibility and alters the concentration of microbial protein in the small intestine or large intestine by pigs fed a corn-soybean meal diet or a diet based on corn, soybean meal, and distillers dried grains with solubles (DDGS). Twenty-four barrows (33.3 ± 3.4 kg) that had a T-cannula installed in the distal ileum were allotted to a 2 × 2 factorial design with 2 levels of DDGS (0% or 45%) and 2 levels of supplemental Cu from Cu hydroxychloride (0 or 150 mg/kg). A 2-period switch back design with the 4 diets and 6 replicate pigs per diet in each period was used resulting in 12 replicate pigs per diet for the 2 periods. The initial 9 d of each period was considered an adaptation period to the experimental diets. For each period, feces were collected on days 10, 11, and 12, and ileal digesta were collected for 8 h on days 13 and 14. Results indicated that inclusion of 45% DDGS to diets reduced (P &lt; 0.05) the apparent ileal digestibility (AID) of AA and the AID and the apparent total tract digestibility (ATTD) of dry matter, gross energy, and crude protein. In contrast, inclusion of DDGS to diets increased (P &lt; 0.05) the AID and the ATTD of acid hydrolyzed ether extract and the concentration of microbial protein in the hindgut (P &lt; 0.05). However, the total concentration of volatile fatty acids (VFA) in ileal digesta and in feces from pigs fed the DDGS diets were not different from concentrations in pigs fed diets without DDGS. The AID and ATTD of dry matter, gross energy, and crude protein were not affected by dietary Cu concentrations, but the AID and ATTD of acid hydrolyzed ether extract were greater (P &lt; 0.05) in diets supplemented with Cu hydroxychloride compared with diets without Cu hydroxychloride. There was also a reduction (P &lt; 0.05) in the concentration of microbial protein and a tendency for a reduction (P &lt; 0.10) in the total concentration of VFA in feces when diets were supplemented with Cu hydroxychloride. In conclusion, supplementation of Cu hydroxychloride to diets improved AID and ATTD of acid hydrolyzed ether extract and reduced the concentration of microbial protein in the large intestine and this effect was observed in diets containing DDGS as well as in diets without DDGS.

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