scholarly journals Screening of Wild-Type Fungal Isolates for Cellulolytic Activity

2011 ◽  
Vol 4 ◽  
pp. MBI.S6418 ◽  
Author(s):  
T.D. Doolotkeldieva ◽  
S.T. Bobusheva
Keyword(s):  
Agricultural Waste ◽  
Mineral Elements ◽  
Cellulolytic Enzymes ◽  
Cellulolytic Activity ◽  
Wild Type ◽  
Waste Products ◽  
Fungal Isolates ◽  
Cotton Oil ◽  
Glucose Reduction ◽  
Penicillium Strain

Background In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzyme production by the fungal isolates was detected within 14 days of cultivation. Results Of 17 strains of Trichoderma, Aspergillus, and Penicillium tested, we identified Penicillium strain K-2-25 and Trichoderma lignorium strain T-22 to have high cellulolytic activity. K-2-25 demonstrated the highest activity after 48 hours of cultivation. T-22 also showed significant cellulolytic activity. Penicillium strain K-2-25 showed cellulolytic activity for 98–270 hours during cultivation, and the amount of reduced glucose was 945 mg. T. lignorium T-22 was the second most active strain, with glucose reduction of 835 mg. Conclusion The strains K-2-25 and T-22 will are be recommended for biotechnological applications, especially for bioconversion of poor hardly decomposable vegetable waste products, such as like straw, into useful biomass.

Bioethanol Production of Cellulase Producing Bacteria from Soils of Agrowaste Field

2021 ◽  
Vol 13 (2) ◽  
pp. 643-655
Author(s):  
A. Thomas ◽  
M. Laxmi ◽  
A. Benny
Keyword(s):  
Treatment Process ◽  
Agricultural Waste ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Bioethanol Production ◽  
Ethanolic Fermentation ◽  
Cellulose Bioconversion ◽  
Congo Red Staining ◽  
Pre Treatment ◽  
And Staphylococcus Aureus

With decades of studies on cellulose bioconversion, cellulases have been playing an important role in producing fermentable sugars from lignocellulosic biomass. Copious microorganisms that are able to degrade cellulose have been isolated and identified. The present study has been undertaken to isolate and screen the cellulase producing bacteria from soils of agrowaste field. Cellulase production has been qualitatively analyzed in carboxy methylcellulose (CMC) agar medium after congo red staining and NaCl treatment by interpretation with zones around the potent colonies. Out of the seven isolates, only two showed cellulase production. The morphogical and molecular characterization revealed its identity as Escherichia coli and Staphylococcus aureus. The potential of organisms for bioethanol production has been investigated using two substrates, namely, paper and leaves by subjecting with a pre-treatment process using acid hydrolysis to remove lignin which acts as physical barrier to cellulolytic enzymes. Ethanolic fermentation was done using Saccharomyces cerevisiae for 24-48 h and then the bioethanol produced was qualitatively proved by iodoform assay. These finding proves that ethanol can be made from the agricultural waste and the process is recommended as a means of generating wealth from waste.

scholarly journals An Efficient and Improved Methodology for the Screening of Industrially Valuable Xylano-Pectino-Cellulolytic Microbes

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Avtar Singh ◽  
Amanjot Kaur ◽  
Anita Dua ◽  
Ritu Mahajan
Keyword(s):  
Low Cost ◽  
Cost Effective ◽  
Industrial Sector ◽  
Agricultural Residues ◽  
High Yield ◽  
Cellulolytic Enzymes ◽  
Cellulolytic Activity ◽  
Nutrient Media ◽  
First Report ◽  
Primary Screening

Xylano-pectino-cellulolytic enzymes are valuable enzymes of the industrial sector. In our earlier study, we have reported a novel and cost effective methodology for the qualitative screening of cellulase-free xylano-pectinolytic microorganisms by replacing the commercial, highly expensive substrates with agricultural residues, but the microorganisms with xylanolytic, pectinolytic, cellulolytic, xylano-pectinolytic, xylano-cellulolytic, pectino-cellulolytic, and xylano-pectino-cellulolytic potential were obtained. The probability of getting the desired combination was low, so efforts were made to further improve this cost effective methodology for obtaining the high yield of the microbes capable of producing desired combination of enzymes. By inclusion of multiple enrichment steps in sequence, using only practically low cost substrates and without any nutrient media till primary screening stage, this improved novel protocol for screening gave only the desired microorganisms with xylano-pectino-cellulolytic activity. Using this rapid, efficient, cost effective, and improved methodology, microbes with required combination of enzymes can be obtained and the probability of getting the desired microorganisms is cent percent. This is the first report presenting the methodology for the isolation of xylano-pectino-cellulolytic positive microorganisms at low cost and consuming less time.

scholarly journals Conversion of Waste Agriculture Biomass to Bioethanol by Recombinant Saccharomyces cerevisiae

2010 ◽  
Vol 2 (2) ◽  
pp. 351-361
Author(s):  
A. A. Saleh ◽  
S. Hamdan ◽  
N. Annaluru ◽  
S. Watanabe ◽  
M. R. Rahman ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzyme Activity ◽  
Protein Engineering ◽  
Agricultural Waste ◽  
Xylitol Dehydrogenase ◽  
Ethanol Conversion ◽  
Waste Biomass ◽  
Wild Type ◽  
Coenzyme Specificity ◽  
Recombinant Yeasts

Agricultural waste biomass has already been transferred to bioethanol and used as energy related products, although many issues such as efficiency and productivity still to be overcome. In this study, the protein engineering was applied to generate enzymes with completely reversed coenzyme specificity and developed recombinant yeasts containing those engineered enzymes for construction of an efficient biomass-ethanol conversion system. Recombinant yeasts were constructed with the genes encoding a wild type xylose reductase (XR) and the protein engineered xylitol dehydrogenase (XDH) (with NADP) of Pichia stipitis.  These recombinant yeasts were characterized based on the enzyme activity and fermentation ability of xylose to ethanol. The protein engineered enzymes were expressed significantly in Saccharomyces cerevisiae as judged by the enzyme activity in vitro. Ethanol fermentation was measured in batch culture under anaerobic conditions. The significant enhancement was found in Y-ARS strain, in which NADP+-dependent XDH was expressed; 85% decrease of unfavorable xylitol excretion with 26% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.  Keywords: Agricultural waste biomass; Protein engineering; Xylitol dehydrogenase; Xylose-fermentation; Eethanol production. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i2.2882               J. Sci. Res. 2 (2), 351-361 (2010) 

scholarly journals Overview of the Influence of Burning Temperature and Grinding Time to the Properties of Cementitious Material based Agricultural Waste Products

2018 ◽  
Vol 431 ◽  
pp. 082004
Author(s):  
Adrina Rosseira ◽  
Noor Nabilah Sarbini ◽  
Izni Syahrizal Ibrahim ◽  
Nor Hasanah Abdul Shukor Lim ◽  
Abdul Rahman Mohd Sam ◽  
...  
Keyword(s):  
Agricultural Waste ◽  
Cementitious Material ◽  
Waste Products ◽  
Burning Temperature ◽  
Grinding Time

scholarly journals Pyramiding Unmarked Deletions in Ralstonia solanacearum Shows That Secreted Proteins in Addition to Plant Cell-Wall-Degrading Enzymes Contribute to Virulence

2005 ◽  
Vol 18 (12) ◽  
pp. 1296-1305 ◽  
Author(s):  
Huanli Liu ◽  
Shuping Zhang ◽  
Mark A. Schell ◽  
Timothy P. Denny
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Ralstonia Solanacearum ◽  
Plant Cell Wall ◽  
Secreted Proteins ◽  
Cellulolytic Enzymes ◽  
Wild Type ◽  
Cell Wall Degrading Enzymes ◽  
Tomato Plants ◽  
Degrading Enzymes

Ralstonia solanacearum, like many phytopathogenic bacteria, makes multiple extracellular plant cell-wall-degrading enzymes (CWDE), some of which contribute to its ability to cause wilt disease. CWDE and many other proteins are secreted to the milieu via the highly conserved type II protein secretion system (T2SS). R. solanacearum with a defective T2SS is weakly virulent, but it is not known whether this is due to absence of all the CWDE or the loss of other secreted proteins that contribute to disease. These alternatives were investigated by creating mutants of wild-type strain GMI1000 lacking either the T2SS or up to six CWDE and comparing them for virulence on tomato plants. To create unmarked deletions, genomic regions flanking the target gene were polymerase chain reaction (PCR)-amplified, were fused using splice overlap extension PCR, were cloned into a suicide plasmid harboring the sacB counter-selectable marker, and then, were site-specifically introduced into the genome. Various combinations of five deletions (δpehA, δpehB, δpehC, δpme, and δegl) and one inactivated allele (cbhA::aphA-3) resulted in 15 mutants missing one to six CWDE. In soil-drench inoculation assays, virulence of mutants lacking only pectic enzymes (PehA, PehB, PehC, and Pme) was not statistically different from GMI1000, but all the mutants lacking one or both cellulolytic enzymes (Egl or CbhA) wilted plants significantly more slowly than did the wild type. The GMI-6 mutant that lacks all six CWDE was more virulent than the mutant lacking only its two cellulolytic enzymes, and both were significantly more virulent than the T2SS mutant (GMI-D). Very similar results were observed in wounded-petiole inoculation assays, so GMI-6 and GMI-D appear to be less capable of colonizing tomato tissues after invasion. Because the T2SS mutant was much less virulent than the sixfold CWDE mutant, we conclude that other secreted proteins contribute substantially to the ability of R. solanacearum GMI1000 to systemically colonize tomato plants.

scholarly journals The Review of Biomass Potential for Agricultural Biogas Production in Poland

2019 ◽  
Vol 11 (22) ◽  
pp. 6515 ◽  
Author(s):  
Katarzyna Anna Koryś ◽  
Agnieszka Ewa Latawiec ◽  
Katarzyna Grotkiewicz ◽  
Maciej Kuboń
Keyword(s):  
Rural Areas ◽  
Food Industry ◽  
Biogas Production ◽  
Agricultural Waste ◽  
Storage Conditions ◽  
Waste Products ◽  
Biomass Residues ◽  
Biogas Plants ◽  
Biomass Potential ◽  
Food Residues

Adequate management of biomass residues generated by agricultural and food industry can reduce their negative impacts on the environment. The alternative use for agricultural waste is production of biogas. Biomass feedstock intended as a substrate for the agricultural biogas plants may include energy crops, bio-waste, products of animal and plant origin and organic residues from food production. This study reviews the potential of selected biomass residues from the agri-food industry in terms of use for agricultural biogas production in Poland. The most common agri-food residues used as substrates for biogas plants in Poland are maize silage, slurry, and distillery waste. It is important that the input for the agricultural biogas installations can be based on local wastes and co-products that require appropriate disposal or storage conditions and might be burdensome for the environment. The study also discusses several limitations that might have an unfavourable impact regarding biogas plants development in Poland. Given the estimated biomass potential, the assumptions defining the scope of use of agricultural biogas and the undeniable benefits provided by biogas production, agricultural biogas plants should be considered as a promising branch of sustainable electricity and thermal energy production in Poland, especially in rural areas.

scholarly journals Transplacental Uptake of Glucose Is Decreased in Embryonic Lethal Connexin26-deficient Mice

1998 ◽  
Vol 140 (6) ◽  
pp. 1453-1461 ◽  
Author(s):  
Heinz-Dieter Gabriel ◽  
Dirk Jung ◽  
Christoph Bützler ◽  
Achim Temme ◽  
Otto Traub ◽  
...  
Keyword(s):  
Placental Transfer ◽  
Maternal Blood ◽  
Wild Type ◽  
Waste Products ◽  
Morphological Alterations ◽  
Gene Coding ◽  
Junctional Protein ◽  
Chorioallantoic Placenta ◽  
Gap Junctional ◽  
Glucose Analogue

Mice that harbor a targeted homozygous defect in the gene coding for the gap junctional protein connexin26 died in utero during the transient phase from early to midgestation. From day 10 post coitum onwards, development of homozygous embryos was retarded, which led to death around day 11 post coitum. Except for growth retardation, no gross morphological alterations were detected between homozygous connexin26-defective embryos and wild-type littermates. At day 9 postcoitum, when chorioallantoic placenta started to function, connexin26 was weakly expressed in the yolk sac epithelium, between syncytiotrophoblasts I and II in the labyrinth region of the placenta, and in the skin of the embryo. At day 10 post coitum, expression of connexin26 in the placenta was much stronger than at the other locations. To analyze involvement of connexin26 in the placental transfer of nutrients, we have measured embryonic uptake of the nonmetabolizable glucose analogue 3-O-[14C]methylglucose, injected into the maternal tail vein. At day 10 post coitum, viable, homozygous connexin26-defective embryos accumulated only ∼40% of the radioactivity measured in wild-type and heterozygous littermates of the same size. We conclude that the uptake of glucose, and presumably other nutrients as well, from maternal blood into connexin26-deficient mouse embryos was severely impaired and apparently not sufficient to support the rapid organogenesis during midgestation. Our results suggest that connexin26 gap junction channels likely fulfill an essential role in the transfer of maternal nutrients and embryonic waste products between syncytiotrophoblast I and II in the labyrinth layer of the mouse placenta.

Effect of Milling Process for Rice Husk Ash on Mechanical Strength of Blended Portland Cement Mortars

2014 ◽  
Vol 600 ◽  
pp. 240-249
Author(s):  
Everton Jose da Silva ◽  
Maria Lidiane Marques ◽  
Antonio Rogério B. Vasconcelos ◽  
Jorge L. Akasaki ◽  
Mauro M. Tashima ◽  
...  
Keyword(s):  
Rice Husk ◽  
Rice Husk Ash ◽  
Agricultural Waste ◽  
Research Area ◽  
Milling Process ◽  
Low Carbon ◽  
Waste Products ◽  
Pozzolanic Reactivity ◽  
Cement Mortars ◽  
Sugar Cane Bagasse Ash

Nowadays, the reuse of waste products in the construction process is a priority research area. Several industrial and agricultural waste products have been investigated, such as fly ash, sugar cane bagasse ash and rice husk ash. This paper analyzes a very important aspect under intense discussion in the scientific community: the Rice Husk Ash (RHA) grinding process. This paper investigates a low carbon RHA with high pozzolanic reactivity produced under uncontrolled burning conditions. The compressive strength of mortar specimens prepared using both ground and natural RHA were tested for 3-56 days and the capillarity absorption was measured for mortars cured during 28 days. Very promising and interesting results were obtained using natural rice husk ash in the production of blended mortars.

scholarly journals EXPERIMENTAL STUDY ON BAGASSE ASH IN CONCRETE

2010 ◽  
Vol 5 (2) ◽  
pp. 60-66 ◽  
Author(s):  
R SRINIVASAN ◽  
K Sathiya
Keyword(s):  
Sugar Cane ◽  
Agricultural Waste ◽  
Waste Product ◽  
Ethanol Vapor ◽  
Sugar Cane Bagasse ◽  
Hardened Concrete ◽  
Split Tensile Strength ◽  
Waste Products ◽  
Compaction Factor ◽  
Sugar Cane Bagasse Ash

Utilization of industrial and agricultural waste products in the industry has been the focus of research for economical, environmental, and technical reasons. Sugar-cane bagasse is a fibrous waste-product of the sugar refining industry, along with ethanol vapor. This waste-product (Sugar-cane Bagasse ash) is already causing serious environmental pollution which calls for urgent ways of handling the waste. Bagasse ash mainly contains aluminum ion and silica. In this paper, Bagasse ash has been chemically and physically characterized, and partially replaced in the ratio of 0%, 5%, 15% and 25% by weight of cement in concrete. The Fresh concrete tests like compaction factor test, Slump cone test was obtained and hardened concrete tests like compressive strength, split tensile strength, flexural strength and Modulus of Elasticity at the age of 7 and 28 days was obtained. The result shows the increases in percentage of bagasse ash replacement, strength also increased.

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