Cryopreservation and Cell Banking for Autologous Mesenchymal Stem Cell-Based Therapies

2013 ◽  
pp. 1 ◽  
Author(s):  
Harel ◽  
Harel
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Banking

scholarly journals Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies

2019 ◽  
Vol 28 (11) ◽  
pp. 1404-1419
Author(s):  
Roger Esteban-Vives ◽  
Jenny Ziembicki ◽  
Myung Sun Choi ◽  
R. L. Thompson ◽  
Eva Schmelzer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Dermal Fibroblasts ◽  
Stem Cell Population ◽  
Differentiation Potential ◽  
Isolation And Characterization ◽  
Cell Banking ◽  
Lineage Stability

Various cell-based therapies are in development to address chronic and acute skin wound healing, for example for burns and trauma patients. An off-the-shelf source of allogeneic dermal cells could be beneficial for innovative therapies accelerating the healing in extensive wounds where the availability of a patient’s own cells is limited. Human fetal-derived dermal fibroblasts (hFDFs) show high in vitro division rates, exhibit low immunological rejection properties, and present scarless wound healing in the fetus, and previous studies on human fetal tissue-derived cell therapies have shown promising results on tissue repair. However, little is known about cell lineage stability and cell differentiation during the cell expansion process, required for any potential therapeutic use. We describe an isolation method, characterize a population, and investigate its potential for cell banking and thus suitability as a potential product for cell grafting therapies. Our results show hFDFs and a bone marrow-derived mesenchymal stem cell (BM-MSC) line shared identification markers and in vitro multilineage differentiation potential into osteogenic, chondrogenic, and adipogenic lineages. The hFDF population exhibited similar cell characteristics as BM-MSCs while producing lower pro-inflammatory cytokine IL-6 levels and higher levels of the wound healing factor hepatocyte growth factor. We demonstrate in vitro differentiation of hFDFs, which may be a problem in maintaining long-term lineage stability, potentially limiting their use for cell banking and therapy development.

Comparison of alternative mesenchymal stem cell sources for cell banking and musculoskeletal advanced therapies

2011 ◽  
Vol 112 (5) ◽  
pp. 1418-1430 ◽  
Author(s):  
Carola Cavallo ◽  
Carmela Cuomo ◽  
Sara Fantini ◽  
Francesca Ricci ◽  
Pier Luigi Tazzari ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Advanced Therapies ◽  
Cell Banking ◽  
Cell Sources

scholarly journals Concise review: Umbilical cord derived mesenchymal stem cell bank

2017 ◽  
Vol 4 (3-4) ◽  
pp. 228-233
Author(s):  
Saharah Nievaleve
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
Disease Treatment ◽  
Cell Bank ◽  
Cell Banking ◽  
Long Time ◽  
Single Cell Culture ◽  
Stem Cell Banking

Mesenchymal stem cells (MSCs) are the most promising stem cells for clinical applications. MSCs are widely used in disease treatment in some different countries. Recently, some MSC banks are developed to cryopreserve MSCs from umbilical cord tissues, adipose tissues and bone marrow. This review aims to discuss some techniques, some advantages as well as disadvantages of umbilical cord derived mesenchymal stem cell banking (UCMSCB). From 2010 to date, there are more than 10 UCMSCBs established in the world. There are two methods to isolate UCMSCs including tissue culture and single cell culture. Then they are cryopreserved in the liquid nitrogen for long time. Although, UCMSCBs can provide more the choice to store the MSCs from umbilical cord, allogenic MSC transplantation with high efficacy in disease treatment suggests that UCMSCBs should change the with new approaches to use the cryopreserved samples.

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