scholarly journals Elevated Serum Sorbitol and not Fructose in Type 2 Diabetic Patients

2010 ◽  
Vol 5 ◽  
pp. BMI.S4530 ◽  
Author(s):  
Gregory M. Preston ◽  
Roberto A. Calle
Keyword(s):  
Elevated Serum ◽  
Fold Increase ◽  
Clinical Development ◽  
Gas Chromatography Mass Spectrometry ◽  
Diabetic Patients ◽  
Dose Selection ◽  
Type 2 Diabetic Patients ◽  
Early Proof ◽  
Proof Of Mechanism

Reductions in fasting serum fructose or erythrocyte sorbitol have been proposed as markers for early proof of mechanism in clinical development of aldose reductase (AR) inhibitors. However fructose is significantly impacted by meals and evaluation of erythrocyte sorbitol poses technical challenges. To more accurately assess the performance of these markers in biological samples, a gas chromatography-mass spectrometry assay was modified and validated. Serum was collected on three consecutive days from 13 healthy volunteers (HV) and 14 patients with type 2 diabetes mellitus (T2DM), and assayed for sorbitol and fructose using this assay. Serum fructose and sorbitol were relatively constant across the three days. Fasting fructose levels were comparable between the two groups (T2DM: 1.48 ± 0.49 mg/L; HV: 1.39 ± 0.38 mg/L, mean ± standard deviation, P = 0.61), but fasting sorbitol levels were significantly higher in diabetics (T2DM: 0.280 ± 0.163 mg/L; HV: 0.164 ± 0.044 mg/L, P = 0.02). Feeding resulted in a 5–6 fold increase in serum fructose levels, but only a 5%-10% increase in sorbitol. Only sorbitol remained significantly elevated pre- and post feeding in T2DM patients relative to HV. These data suggest that serum sorbitol may be a robust proof of mechanism biomarker and facilitate dose selection for clinical development of AR inhibitors.

Elevated serum leptin concentrations in type 2 diabetic patients with microalbuminuria and macroalbuminuria

Metabolism ◽  
1999 ◽  
Vol 48 (10) ◽  
pp. 1290-1293 ◽  
Author(s):  
Bernd Fruehwald-Schultes ◽  
Werner Kern ◽  
Jürgen Beyer ◽  
Thomas Forst ◽  
Andreas Pfützner ◽  
...  
Keyword(s):  
Elevated Serum ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Serum Leptin ◽  
Type 2 Diabetic

CORRELATION BETWEEN SERUM CYSTATIN C LEVEL AND RENAL FUNCTION IN PREDIABETES, TYPE 2 DIABETIC PATIENTS

2017 ◽  
pp. 164-173
Author(s):  
Anh Dao Dang ◽  
Huu Dang Tran
Keyword(s):  
Cystatin C ◽  
Healthy Subjects ◽  
Filtration Rate ◽  
Elevated Serum ◽  
Diabetic Patients ◽  
Serum Cystatin C ◽  
Type 2 Diabetic Patients ◽  
Serum Cystatin ◽  
Type 2 Diabetic

Objectives: To investigate serum Cystatin C level, percentage of elevated serum cystatin C in prediabetic, type 2 diabetic patients. Relationship between serum Cystatin C levels and albuminuria in prediabetes. Correlation between serum Cystatin C levels and estimated glomerular filtration rate (eGFR), renal radiography in type 2 diabetic patients. Materials and methods: 90 healthy subjects, 60 patients with prediabetes, and 124 type 2 diabetic patients were inrolled into this study. We excluded patients with thyroid dysfunction and those taking glucocorticoids that affect the serum Cystatin level. The serum Cystatin C and Creatinine, renal radiography, albuminuria, and eGFR using CKD.EPI, MDRD formular were calculated. Descriptive and cross-sectional study. Analyzed correlation, Receiver operating characteristics (ROC), calculated the area under the curve (AUC) for serum Cystatin C. Results: The level of serum Cystatin C gradually significantly increased in healthy subjects, prediabetes, and type 2 diabetic patients (0.848±0.097; 0.894±0.113; 1.410±0.900 mg/L, p<0.05, respectively). Percentage of elevated serum cystatin C in prediabetic and type 2 diabetic patients were 13%, 47.6%, p<0.05, respectively. There was a positive correlation of serum cystatin C with albuminuria, a negatively correlation between serum Cystatin C level and eGFR in prediabetic subjects.There was a strong positively correlation of eGFR according to CKD.EPI equation based on serum Cystatin C (r=0.804, p < 0.001) and renal radiography (r=0.767, p<0.001). The strong positively correlation between eGFR based on serum Creatinine-Cystatin C and renal radiography (r=0.804, p<0.001). Serum Cystatin C level was significantly negatively correlation with eGFR based on cystatin C serum (r=-0.861, p<0.001) and renal radiography (r=0.739 p<0.001). The cut off value for the identification of GFR < 60ml/min/1.73m2 was 1.53mg/l with a sensitivity of 93.6 (95%CI: 78.3- 96.3) and specificity of 97.4 (95%CI: 88.0- 98.2), AUC was 0.96 according to renal radiography.The cutoff value for the identification of GFR < 60ml/min/1.73m2 was 1.3mg/l with a sensitivity of 100,0 (95%CI: 91.3- 100.0) and specificity of 94.0 (95%CI: 86.5- 98,0), AUC was 0,989 according to CKD.EPI formular based on Cystatin C. Conclusions: The level of serum Cystatin C gradually significantly increased in healthy subjects, prediabetes, and type 2 diabetic patients. There was a correlation of serum cystatin C with albuminuria, eGFR in prediabetic subjects. There was a positive correlation between eGFR based on Cystatin C and renal radiography. A negativelysignificantly correlation between serum Cystatin C level and eGFR,renal radiography. Serum Cystatin C had strong sensitivityand specificity for detecting reduced GFR. Key words: prediabetes, diabetes mellitus, glomerular filtration rate, Cystatin C, albuminuria

scholarly journals ACE gene polymorphism and serum ACE level with Progression of Nephropathy in Type 2 Diabetic Patients

2013 ◽  
Vol 9 (3) ◽  
pp. 2023-2032
Author(s):  
Olfat Gamil Shaker ◽  
Manal Fouad Ismail ◽  
Esmat Ashour ◽  
Heba Mourad Yousif ◽  
Mie Afify ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Strong Association ◽  
Elevated Serum ◽  
Renin Angiotensin System ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Ace Gene ◽  
Ace Gene Polymorphism ◽  
Type 2 Diabetic

Background. One of the most common complications of diabetes mellitus (DM) is diabetic nephropathy (DN).  Angiotensin- converting enzyme (ACE) gene was the first candidate gene of renin-angiotensin system (RAS) for predisposition to DN.Objective. Investigation whether the ACE insertion/deletion (I/D) polymorphism is associated with Egyptian type 2 diabetic patients (T2DM) with nephropathy. In addition, the study investigated the relationship between variants of ACE I/D gene polymorphism and serum ACE level and the progression of nephropathy in Egyptian T2DM patients.Methods.  A total of 85 T2DM patients (45 with nephropathy and 40 without nephropathy) besides 45 healthy (non-diabetic) age-matched subjects were recruited in this study for comparison. The (I/D) polymorphism of the ACE gene was investigated using PCR and serum ACE levels were determined using ELISA.Results. The frequency of ACE DD genotype and D allele was significantly higher in DN patients when compared to control healthy subjects and diabetic patients without nephropathy. In addition our results showed a significant association between DD genotype of ACE gene and elevated serum ACE level.Conclusion. The present study showing a strong association between the D allele and/or DD homozygous of ACE gene and diabetic patients developed nephropathy. In addition, individuals with D allele have higher levels of serum ACE compared to those having I allele. ACE gene polymorphism and serum ACE level may serve as a susceptibility biomarker for nephropathy in type 2 diabetic patients. 

scholarly journals Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status

IUBMB Life ◽  
2017 ◽  
Vol 69 (2) ◽  
pp. 63-71 ◽  
Author(s):  
Yuanyuan Li ◽  
Wenzhuo Ran ◽  
Jiaqiang Zhang ◽  
Shi Chen ◽  
Yihang Li ◽  
...  
Keyword(s):  
Growth Factor ◽  
Milk Fat ◽  
Elevated Serum ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Milk Fat Globule ◽  
Epidermal Growth ◽  
Fat Globule ◽  
Type 2 Diabetic

Elevated serum lipoprotein(a) levels in type 1 and type 2 diabetic patients among malaysian malays

2001 ◽  
Vol 2 (2) ◽  
pp. 56
Author(s):  
H.M. Nawawi ◽  
M. Muhajir ◽  
C.K. Yeo ◽  
W.M. Wan Nazaimoon ◽  
K. Yusoff ◽  
...  
Keyword(s):  
Elevated Serum ◽  
Serum Lipoprotein ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Lipoprotein A ◽  
Type 2 Diabetic

scholarly journals Elevated Serum Uric Acid Concentrations Independently Predict Cardiovascular Mortality in Type 2 Diabetic Patients

Diabetes Care ◽  
2009 ◽  
Vol 32 (9) ◽  
pp. 1716-1720 ◽  
Author(s):  
G. Zoppini ◽  
G. Targher ◽  
C. Negri ◽  
V. Stoico ◽  
F. Perrone ◽  
...  
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Cardiovascular Mortality ◽  
Elevated Serum ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

scholarly journals Frequency of Dyslipidemia In Type 2 Diabetic Patients In Karachi

2019 ◽  
Vol 09 (02) ◽  
pp. 133-136
Author(s):  
Sehrish Shafique ◽  
Daud Mirza ◽  
Summayya Shawana ◽  
Shahneela Tabassum ◽  
Naveed Faraz
Keyword(s):  
Low Density Lipoprotein ◽  
Elevated Serum ◽  
Density Lipoprotein ◽  
Cross Sectional Study ◽  
P Value ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Cross Sectional ◽  
Type 2 Diabetic

Objective: To find out the frequency of dyslipidemias in type 2 diabetic patients. Study Design and Setting: This was cross-sectional study and conducted in a different clinics in Karachi during 3 months period. Methodology: Those patients who fulfilled the inclusion criteria and attended the OPD were selected. Patient were advised to bring their lipid profile report in next visit if not already done. The total dyslipidemias were presented by their frequencies and percentages with 95% confidence interval. Results: Out of 383 patients with diabetes mellitus 210 (55 %) had dyslipidemia. Among which 76% had elevated lowdensity lipoprotein (LDL), 66.6 % had elevated serum cholesterol, 57% patient had elevated triglyceride. p- value of 0.05 was taken as statistically significant. Conclusion: It was concluded that increased frequency of dyslipidemias, with elevated low-density lipoprotein, cholesterol and triglycerides levels seen in diabetic patients.

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