scholarly journals Bioprospecting Kitchen Refuse as a Suitable Substrate for Biogasification

2016 ◽  
Vol 9 ◽  
pp. ASWR.S32779 ◽  
Author(s):  
Vijay K. Malesu ◽  
Snehasish Mishra ◽  
Sanjay K. Ojha ◽  
Kalyani Naik ◽  
Puneet K. Singh ◽  
...  

Conventionally, methane nonproducing organic substrates such as kitchen refuse (KR) are amenable as biogasifiers, similar or even better than that of the naturally biogasifying cow dung (CD) through process modification. Comparative physicochemical and biological analyses revealed that KR had no methanogen and was low on amylase and cellulase positive and total microbial counts. It was observed that the pH level lowered further when the KR alone was biogasified, attributable to the accumulating volatile fatty acids, which indicates the failure of the last and final step of biomethanation. Study of the raw and digested forms of KR, CD, and kitchen refuse fortified with cow dung (KC) revealed that there was a net percentage decrease in dry matter (70.00, 94.33, and 88.88, respectively), total dissolved solids (1, 1.5, and 1.5, respectively), and phosphate contents (12, 19, and 20, respectively), indicating an optimal microbial activity in all the substrates. Although digestion rate in CD was better than that in KR, KC exhibited an enhanced digestion rate over KR attributable to the process being facilitated by increased microbial counts; amylase-, cellulase-, and lipase-positive microbes; and methanogens. Furthermore, the active methanogens in CD inoculum (in KC) facilitated biomethanation by better utilizing the volatile fatty acids that ensured better stability in the pH level throughout. The cumulative biogas production values were 1281, 4448, and 3256 cm3 in KR, CD, and KC, respectively. Methane production started by the seventh day in CD and KC and reached up to 63.65% and 53%, respectively, by the 21st day in batch operation. Thus, KR is a promising candidate for biogasification, thereby opening a plethora of opportunity to utilize the technology even in urban and periurban locations that are low on cattle resources albeit rich in other organic refuse. There is a necessity to estimate the biomethanation potentials of various other available organic refuse.

Author(s):  
V. L. Poliakov

A mathematical problem of the action of a representative biofilm in the absence of oxygen is formulated. The anaerobic process of decomposition of a dissolved organic matter is considered as a two-stage process, proceeding due to the vital activity of two groups of microorganisms. An approximate analytic solution allowing one to calculate the concentration and consumption of primary and secondary organic substrates with minimal errors has been obtained. On test examples, their rates of transfer through the biofilm surface are determined, and the possibility of the movement of volatile fatty acids in both directions is discussed.


1984 ◽  
Vol 64 (5) ◽  
pp. 167-168 ◽  
Author(s):  
S. OSHIO ◽  
I. TAHATA

The fractional absorption rate of VFA, which was in the order of caproic > valeric > butyric = propionic = acetic > isovaleric > isobutyric acid at high pH level, was promoted in hypotonic solution. Conversely in hypertonic solution or long-term isolated rumen pouch, the absorption of lower acids such as acetic or propionic acid was depressed. Key words: VFA, absorption, osmolality, rumen, sheep


1956 ◽  
Vol 2 (6) ◽  
pp. 559-563 ◽  
Author(s):  
P. Shu ◽  
A. C. Neish ◽  
G. A. Ledingham

Uredospores of wheat stem rust utilized a number of externally-supplied, labelled carbohydrates, amino acids, and volatile fatty acids. The carbon of these substrates appeared in the spore materials and in the carbon dioxide. This metabolic activity, though very weak, is definite. D-Mannose, D-mannitol, D-glucose, sucrose, and D-fructose were utilized better than the other carbohydrates. Glutamic acid gave the highest yield of carbon dioxide while the basic amino acids, L-arginine and L-lysine, were more efficiently incorporated into the spore material.


2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.


МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY


1962 ◽  
Vol 21 (1) ◽  
pp. 37-40 ◽  
Author(s):  
H. W. Essig ◽  
U. S. Garrigus ◽  
B. Connor Johnson

Ge-Bu ◽  
2020 ◽  
Vol 79-84 (6-7) ◽  
pp. 1
Author(s):  
CA Van den Bogert

1997 ◽  
Vol 34 (5) ◽  
pp. 349-351
Author(s):  
Hiroshi KAMISOYAMA ◽  
Zeng-Tao SUN ◽  
Mineo HASHIGUCHI ◽  
Yutaka ISSHIKI

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