Optimization of ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharide from radix Morindae officinalis by response surface methodology

2020 ◽  
Vol 16 (71) ◽  
pp. 662
Author(s):  
Xinjun Xu ◽  
Jie Shen ◽  
Zhenying Mei ◽  
Zhiqin Xu ◽  
Zhimin Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Enzymatic Extraction ◽  
Ultrasound Assisted

scholarly journals Simultaneous Optimization of Ultrasound-Assisted Extraction for Flavonoids and Antioxidant Activity of Angelica keiskei Using Response Surface Methodology (RSM)

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3461 ◽  
Author(s):  
Lei Zhang ◽  
Yuhuan Jiang ◽  
Xuening Pang ◽  
Puyue Hua ◽  
Xiang Gao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Biological Activities ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Angelica Keiskei ◽  
Ultrasound Assisted

Angelica keiskei Koidzumi (A. keiskei), as a Japanese edible herbal plant, enjoys a variety of biological activities due to the presence of numerous active compounds, especially flavonoids. This study aims for the optimization of ultrasound-assisted extraction (UAE) for flavonoids in A. keiskei and their antioxidant activity by using the response surface methodology (RSM). Single-factor experiments and a four-factor three-level Box–Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction and antioxidant activity evaluation: ultrasonic temperature (X1), ultrasonic time (X2), ethanol concentration (X3) and liquid–solid ratio (X4). The optimum conditions of the combination of total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity (DPPH-RSC) and ferric-reducing antioxidant power (FRAP) were as follows: X1 = 80 °C, X2 = 4 min, X3 = 78%, X4 = 35 mL/g, respectively. The experimental results provide a theoretical basis for the extensive utilization of A. keiskei and flavonoids extraction from A. keiskei as a potential source of antioxidants.

scholarly journals Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Fujia Chen ◽  
Dahong Li ◽  
Hongqi Shen ◽  
Chunhong Wang ◽  
Enzhong Li ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Average Molecular Weight ◽  
Reducing Power ◽  
Extraction Temperature ◽  
Antitumor Activities ◽  
Enzymatic Extraction ◽  
Solid Ratio ◽  
Natural Medicine ◽  
Ultrasound Assisted

An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides fromTrichosanthes Fructus(crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction. The statistical analysis indicated that the independent variables (X4,X2, andX3), the quadratic coefficients (X12,X22,X32, andX42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest thatTrichosanthes Fructusand TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.

scholarly journals UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Guiqin Shi ◽  
Jiaxin Shen ◽  
Tao Wei ◽  
Fei Ren ◽  
Famou Guo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Paeonia Lactiflora ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasound Assisted

In this study, the ultrasound-assisted extraction (UAE) of flavonoid from Paeonia lactiflora seed peel was optimized by response surface methodology (RSM). Single-factor experiments and a three-factor three-level Box-Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction: ethanol concentration ( X 1 ), liquid-solid ratio ( X 2 ), and ultrasonic time ( X 3 ). The results showed that the optimal flavonoid yield (10.9045 mg RE/g) was as follows: ethanol concentration 62.93%, ultrasonic time 64.56 min, and liquid-solid ratio 24.86 mL/g. The optimized extract of P. lactiflora seed shell was further analyzed by UPLC-ESI-MS/MS, and 20 main flavonoids were identified and quantified, among which protocatechuic acid, vanillic acid, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzaldehyde had the highest content. Furthermore, the results of the antioxidant test showed that the P. lactiflora seed peel extract obtained under optimized UAE conditions exhibited good antioxidant activity. The experimental results showed that ultrasound-assisted extraction was a fast, efficient, and simple method for extracting active ingredients from P. lactiflora seed peel, thereby making this byproduct a promising source of compounds in food and healthcare sectors.

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