A comparative study on antifungal susceptibility of Penicillium marneffei (Talaromyces marneffei) and nonmarneffei Penicillium species

2018 ◽  
Vol 32 (1) ◽  
pp. 22 ◽  
Author(s):  
KhuraijamRanjana Devi ◽  
RandhirBabu Singh
Keyword(s):  
Comparative Study ◽  
Antifungal Susceptibility ◽  
Penicillium Marneffei ◽  
Penicillium Species ◽  
Talaromyces Marneffei

Methylcitrate cycle gene MCD is essential for the virulence of Talaromyces marneffei

2019 ◽  
Vol 58 (3) ◽  
pp. 351-361 ◽  
Author(s):  
Jiao Feng ◽  
Liya He ◽  
Xing Xiao ◽  
Zhiwen Chen ◽  
Chunmei Chen ◽  
...  
Keyword(s):  
Causative Agent ◽  
Carbon Sources ◽  
Penicillium Marneffei ◽  
Systemic Mycosis ◽  
Infection Experiment ◽  
Talaromyces Marneffei ◽  
Free Media ◽  
Macrophage Killing

Abstract Talaromyces marneffei (T. marneffei), which used to be known as Penicillium marneffei, is the causative agent of the fatal systemic mycosis known as talaromycosis. For the purpose of understanding the role of methylcitrate cycle in the virulence of T. marneffei, we generated MCD deletion (ΔMCD) and complementation (ΔMCD+) mutants of T. marneffei. Growth in different carbon sources showed that ΔMCD cannot grow on propionate media and grew slowly on the valerate, valine, methionine, isoleucine, cholesterol, and YNB (carbon free) media. The macrophage killing assay showed that ΔMCD was attenuated in macrophages of mice in vitro, especially at the presence of propionate. Finally, virulence studies in a murine infection experiment revealed attenuated virulence of the ΔMCD, which indicates MCD is essential for T. marneffei virulence in the host. This experiment laid the foundation for the further study of the specific mechanisms underlying the methylcitrate cycle of T. marneffei and may provide suitable targets for new antifungals.

Novel Penicillium species causing disseminated disease in a Labrador Retriever dog

2020 ◽  
Vol 58 (8) ◽  
pp. 1053-1063 ◽  
Author(s):  
Tatiana Rothacker ◽  
Jared A Jaffey ◽  
Erin R Rogers ◽  
William H Fales ◽  
Connie F C Gibas ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Phylogenetic Analyses ◽  
Antifungal Susceptibility ◽  
Clinical Signs ◽  
Labrador Retriever ◽  
Penicillium Species ◽  
Lipid Complex ◽  
The Right ◽  
Disseminated Disease

Abstract This report describes the phenotypic characteristics of a novel Penicillium species, Penicillium labradorum, isolated from a 3-year-old male, castrated, Labrador retriever with disseminated fungal disease. The dog's presenting clinical signs included lethargy, lymphadenopathy, tachypnea, moderate pitting edema, and nonweight bearing lameness associated with the right hind limb. Fine-needle aspirate biopsies from the sublumbar and prescapular lymph nodes were initially examined. The cytologic findings were consistent with pyogranulomatous inflammation with abundant extracellular and phagocytized fungal fragments and hyphae. Based on the morphology of the organisms and lack of endogenous pigment, hyalohyphomycosis was considered most likely, with Fusarium, Penicillium, and Paecilomyces species being considerations. Fungal isolates were obtained via culture of samples from the lymph nodes, and molecular identification testing originally identified an undescribed Penicillium species belonging to the Penicillium section Exilicaulis. BLAST searches and phylogenetic analyses performed approximately 1 year and 9 months after the isolation date revealed an isolate within the Penicillium parvum clade in the Penicillium section Exilicaulis but phylogenetically distant from the other species in the section, thus representing a new species, Penicillium labradorum. Antifungal susceptibility testing was also performed on the isolate and low minimum inhibitory concentrations were observed with terbinafine, voriconazole, and posaconazole, while in vitro resistance was observed with fluconazole. The dog had been previously treated with fluconazole, itraconazole, amphotericin B lipid complex, voriconazole, and terbinafine. Approximately 587 days after the initial diagnosis, the dog was euthanized due to worsening of clinical signs and concerns for quality of life.

scholarly journals HIV-child with disseminated Talaromyces Marneffei (Penicillium marneffei) infection: a rare severe case report

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Wei Hu ◽  
Xiao-hong Yu ◽  
Wei-qin Wei ◽  
Xuan Xiang
Keyword(s):  
Single Case ◽  
Clinical Manifestations ◽  
Severe Case ◽  
Laboratory Diagnosis ◽  
Penicillium Marneffei ◽  
Bloody Stool ◽  
Talaromyces Marneffei ◽  
Left Lower Extremity ◽  
Imaging Results ◽  
Immunodeficiency Syndrome

Abstract Background Talaromyces Marneffei (Penicillium marneffei, T.marneffei) has been frequently reported in patients with adult acquired immunodeficiency syndrome. Still, cases of children with HIV combined with T.marneffei infection are very rare. This report describes the case of a HIV-child who is a girl from China. Her special clinical manifestations and laboratory diagnosis results can provide clinicians with the basis for diagnosis and treatment of T.marneffei related rare diseases. Case presntation We reported a single case of 7-year-old Chinese female patient who presented with fever, abdominal pain, multiple lymphadenopathy, hepatosplenomegaly, left lower extremity ecchymosis, and bloody stool. The patient received anti-inflammatory therapy; however, her symptoms did not improve. Consequently, she was diagnosed with T.marneffei and HIV infection; it was also confirmed that her mother did not undergo HIV blocking therapy during pregnancy. Yet, the child’s family refused all treatment, after which the child was discharged from the hospital. The patient died a few days later. Conclusion This case suggested that children with AIDS suffering from fever, lymphadenopathy and coagulation dysfunction, penicilliosis should be suspected. Clinicians should diagnose the disease early through laboratory and imaging results, which can help reduce the mortality, prolong the survival time and improve the quality of life of children.

AIDS-assoziierte disseminierte Talaromyces marneffei-Mykose (vormals Penicillium marneffei-Mykose)

2018 ◽  
Vol 16 (10) ◽  
pp. 1256-1259
Author(s):  
Qiaofei Li ◽  
Chuqiang Wang ◽  
Kang Zeng ◽  
Xiaoming Peng ◽  
Fang Wang
Keyword(s):  
Penicillium Marneffei ◽  
Talaromyces Marneffei

scholarly journals Antifungal susceptibility profiles of olorofim (formerly F901318), and currently available systemic antifungals against mold and yeast phases of Talaromyces marneffei

2021 ◽  
Author(s):  
Jing Zhang ◽  
Hongfang Liu ◽  
Liyan Xi ◽  
Yun C. Chang ◽  
Kyung J. Kwon-Chung ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Antifungal Agent ◽  
Southern China ◽  
Antifungal Susceptibility ◽  
Talaromyces Marneffei ◽  
Mold And Yeast ◽  
Susceptibility Profiles

In vitro antifungal susceptibility of 32 clinical and environmental Talaromyces marneffei isolates recovered from southern China was performed against olorofim and 7 other systemic antifungals including: amphotericin B, 5-flucytosine, posaconazole, voriconazole, caspofungin and terbinafine using the CLSI methodology. In comparison, olorofim was the most active antifungal agent against both mold and yeast phases of all tested Talaromyces marneffei isolates, exhibiting an MIC range, MIC50, and MIC90 of 0.0005-0.002 μg/ml, 0.0005 μg/ml, and 0.0005 μg/ml, respectively.

Sign in / Sign up

Export Citation Format

Share Document