AIDS-assoziierte disseminierte Talaromyces marneffei-Mykose (vormals Penicillium marneffei-Mykose)

2018 ◽  
Vol 16 (10) ◽  
pp. 1256-1259
Author(s):  
Qiaofei Li ◽  
Chuqiang Wang ◽  
Kang Zeng ◽  
Xiaoming Peng ◽  
Fang Wang
Keyword(s):  
Penicillium Marneffei ◽  
Talaromyces Marneffei

Methylcitrate cycle gene MCD is essential for the virulence of Talaromyces marneffei

2019 ◽  
Vol 58 (3) ◽  
pp. 351-361 ◽  
Author(s):  
Jiao Feng ◽  
Liya He ◽  
Xing Xiao ◽  
Zhiwen Chen ◽  
Chunmei Chen ◽  
...  
Keyword(s):  
Causative Agent ◽  
Carbon Sources ◽  
Penicillium Marneffei ◽  
Systemic Mycosis ◽  
Infection Experiment ◽  
Talaromyces Marneffei ◽  
Free Media ◽  
Macrophage Killing

Abstract Talaromyces marneffei (T. marneffei), which used to be known as Penicillium marneffei, is the causative agent of the fatal systemic mycosis known as talaromycosis. For the purpose of understanding the role of methylcitrate cycle in the virulence of T. marneffei, we generated MCD deletion (ΔMCD) and complementation (ΔMCD+) mutants of T. marneffei. Growth in different carbon sources showed that ΔMCD cannot grow on propionate media and grew slowly on the valerate, valine, methionine, isoleucine, cholesterol, and YNB (carbon free) media. The macrophage killing assay showed that ΔMCD was attenuated in macrophages of mice in vitro, especially at the presence of propionate. Finally, virulence studies in a murine infection experiment revealed attenuated virulence of the ΔMCD, which indicates MCD is essential for T. marneffei virulence in the host. This experiment laid the foundation for the further study of the specific mechanisms underlying the methylcitrate cycle of T. marneffei and may provide suitable targets for new antifungals.

scholarly journals HIV-child with disseminated Talaromyces Marneffei (Penicillium marneffei) infection: a rare severe case report

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Wei Hu ◽  
Xiao-hong Yu ◽  
Wei-qin Wei ◽  
Xuan Xiang
Keyword(s):  
Single Case ◽  
Clinical Manifestations ◽  
Severe Case ◽  
Laboratory Diagnosis ◽  
Penicillium Marneffei ◽  
Bloody Stool ◽  
Talaromyces Marneffei ◽  
Left Lower Extremity ◽  
Imaging Results ◽  
Immunodeficiency Syndrome

Abstract Background Talaromyces Marneffei (Penicillium marneffei, T.marneffei) has been frequently reported in patients with adult acquired immunodeficiency syndrome. Still, cases of children with HIV combined with T.marneffei infection are very rare. This report describes the case of a HIV-child who is a girl from China. Her special clinical manifestations and laboratory diagnosis results can provide clinicians with the basis for diagnosis and treatment of T.marneffei related rare diseases. Case presntation We reported a single case of 7-year-old Chinese female patient who presented with fever, abdominal pain, multiple lymphadenopathy, hepatosplenomegaly, left lower extremity ecchymosis, and bloody stool. The patient received anti-inflammatory therapy; however, her symptoms did not improve. Consequently, she was diagnosed with T.marneffei and HIV infection; it was also confirmed that her mother did not undergo HIV blocking therapy during pregnancy. Yet, the child’s family refused all treatment, after which the child was discharged from the hospital. The patient died a few days later. Conclusion This case suggested that children with AIDS suffering from fever, lymphadenopathy and coagulation dysfunction, penicilliosis should be suspected. Clinicians should diagnose the disease early through laboratory and imaging results, which can help reduce the mortality, prolong the survival time and improve the quality of life of children.

scholarly journals The novel Dbl homology/BAR domain protein, MsgA, of Talaromyces marneffei regulates yeast morphogenesis during growth inside host cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Harshini Weerasinghe ◽  
Hayley E. Bugeja ◽  
Alex Andrianopoulos
Keyword(s):  
Pathogenic Fungi ◽  
Host Cells ◽  
Microbial Pathogens ◽  
Mammalian Host ◽  
Host Immune System ◽  
Nucleotide Exchange ◽  
Phagocytic Cells ◽  
Macrophage Infection ◽  
Bar Domain ◽  
Talaromyces Marneffei

AbstractMicrobial pathogens have evolved many strategies to evade recognition by the host immune system, including the use of phagocytic cells as a niche within which to proliferate. Dimorphic pathogenic fungi employ an induced morphogenetic transition, switching from multicellular hyphae to unicellular yeast that are more compatible with intracellular growth. A switch to mammalian host body temperature (37 °C) is a key trigger for the dimorphic switch. This study describes a novel gene, msgA, from the dimorphic fungal pathogen Talaromyces marneffei that controls cell morphology in response to host cues rather than temperature. The msgA gene is upregulated during murine macrophage infection, and deletion results in aberrant yeast morphology solely during growth inside macrophages. MsgA contains a Dbl homology domain, and a Bin, Amphiphysin, Rvs (BAR) domain instead of a Plekstrin homology domain typically associated with guanine nucleotide exchange factors (GEFs). The BAR domain is crucial in maintaining yeast morphology and cellular localisation during infection. The data suggests that MsgA does not act as a canonical GEF during macrophage infection and identifies a temperature independent pathway in T. marneffei that controls intracellular yeast morphogenesis.

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