scholarly journals Effect of hyaluronic acid added to suture material and its relationship with bacterial colonization: An In vitro study

2018 ◽  
Vol 8 (5) ◽  
pp. 391
Author(s):  
VarmaRama Sudhir ◽  
Thomas Biju ◽  
Amitha Ramesh ◽  
Suleiman Ergieg ◽  
SalimAbou Fanas ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Suture Material ◽  
Bacterial Colonization ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals The Antimicrobial efficacy of 0.8% Hyaluronic Acid and 0.2% Chlorhexidine against Porphyromonas gingivalis strains: An in-vitro study

2019 ◽  
Vol 36 (2) ◽  
Author(s):  
Munerah Binshabaib ◽  
Kawther Aabed ◽  
Fitoon Alotaibi ◽  
Milaf Alwaqid ◽  
Aljohara Alfraidy ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Porphyromonas Gingivalis ◽  
In Vitro Study ◽  
Human Saliva ◽  
Vitro Study ◽  
Antimicrobial Efficacy ◽  
Creative Commons ◽  
Glass Slides ◽  
Group B

Objective: The aim of the present in-vitro study was to assess antimicrobial efficacy of 0.8% hyaluronic acid (HA) and 0.2% Chlorhexidine gluconate (CHX) against Porphyromonas gingivalis (P. gingivalis). Methods: The study was performed between December 2018 and March 2019 at the College of Dentistry at the Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia. The P. gingivalis biofilms were formed and grown for 72 hours at 37°C under anaerobic conditions on glass slides coated with human saliva. The slides were individually positioned and exposed to 0.8% HA or 0.2% CHX. Therapeutically, the biofilms were divided into 3 groups as follows: (a) negative group; (b) 0.8% HA group and (c) 0.2% CHX group. P-values less than 0.05 were considered statistically significant. Results: In the 0.8% HA group, P. gingivalis CFUs/ml were significantly higher at baseline than at 24- (P<0.05), 48 (P<0.05) and 72 hours (P<0.05) intervals. In the 0.2% CHX group, P. gingivalis CFUs/ml were significantly higher at baseline than at 72 hours interval (P<0.05). In the CHX group, there was no difference in P. gingivalis CFUs/ml between baseline, 24- and 48-hours intervals. At 48- and 72-hours intervals, the P. gingivalis CFUs/ml were significantly higher in the 0.2% CHX group compared with the 0.8% HA group. Conclusion: In-vitro, 0.8% HA is more effective in reducing the P. gingivalis CFUs/ml compared with 0.2% CHX. doi: https://doi.org/10.12669/pjms.36.2.1456 How to cite this:Binshabaib M, Aabed K, Alotaibi F, Alwaqid M, Alfraidy A, Alharthi S. Antimicrobial efficacy of 0.8% Hyaluronic Acid and 0.2% Chlorhexidine against Porphyromonas gingivalis strains: An in-vitro study. Pak J Med Sci. 2020;36(2):---------.   doi: https://doi.org/10.12669/pjms.36.2.1456 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Combination of urea-crosslinked hyaluronic acid and sodium ascorbyl phosphate for the treatment of inflammatory lung diseases: An in vitro study

2018 ◽  
Vol 120 ◽  
pp. 96-106 ◽  
Author(s):  
Arianna Fallacara ◽  
Laura Busato ◽  
Michele Pozzoli ◽  
Maliheh Ghadiri ◽  
Hui Xin Ong ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Lung Diseases ◽  
In Vitro Study ◽  
Vitro Study

Characterization of a hyaluronic acid-based hydrogel containing an extracellular oxygen carrier (M101) for periodontitis treatment: an in vitro study

2021 ◽  
pp. 120810
Author(s):  
Hayriye Özçelik ◽  
Fareeha Batool ◽  
Maïwenn Corre ◽  
Alexandre Garlaschelli ◽  
Guillaume Conzatti ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Oxygen Carrier ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Elena Canciani ◽  
Nicoletta Gagliano ◽  
Francesca Paino ◽  
Evžen Amler ◽  
Radek Divin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Vitamin E ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Vitro Study ◽  
Gingival Tissue ◽  
Collagen Deposition ◽  
Dental Practitioners ◽  
In Cells

Aim: The regeneration of small periodontal defects has been considered an important divide and challenging issue for dental practitioners. The aim of this preliminary in vitro study was to analyze the effects of polycaprolactone (PCL) nanofibers enriched with hyaluronic acid and vitamin E vs. nude nanofibers on gingival fibroblasts activity, an innovative graft for periodontal soft tissue regeneration purposes.Methods: Nanofibers were produced in PCL (NF) or PCL enriched with hyaluronic acid and vitamin E (NFE) by electrospinning technique. NF and NFE were stereologically and morphologically characterized by scanning electron microscope (SEM), and composition was analyzed by infrared spectroscopy. Human fibroblasts were obtained from one gingival tissue fragment (HGF) and then seeded on NF, NFE, and plastic (CT). Cell adhesion and morphology were evaluated using SEM at 24 h and cell viability after 24, 48, and 72 h by alamarBlue® assay. Gene expression for COL-I, LH2b, TIMP-1, PAX, and VNC was analyzed by real-time RT-PCR in samples run in triplicate and GAPDH was used as housekeeping gene. Slot blot analysis was performed and immunoreactive bands were revealed for MMP-1 and COL-I. YAP and p-YAP were analyzed by Western blot and membranes were reprobed by α-tubulin. Statistical analysis was performed.Results: IR spectrum revealed the presence of PCL in NF and PCL and vitamin E and hyaluronic acid in NFE. At 24 h, HGF adhered on NF and NFE conserving fibroblast like morphology. At 72 h from seeding, statistically significant differences were found in proliferation of HGF cultured on NF compared to NFE. Expression of genes (LH2b, TIMP-1, and MMP-1) and proteins (COL-I) related to collagen turnover revealed a reduction of COL-1 secretion in cells cultured on NF and NFE compared to CT; however, NFE stimulated cross-linked collagen deposition. Mechanosensor genes (PAX, VNC, and YAP) were upregulated in HGF on NF while they were decreased in cells grown on NFE.Conclusion: Preliminary data suggest that PCL-enriched nanofibers could represent a support to induce HGF proliferation, adhesion, collagen cross-linking, and to reduce collagen degradation, therefore favoring collagen deposition in gingival connective tissue.

Bacterial Colonization of the Dental Implant Fixture–Abutment Interface: An In Vitro Study

2009 ◽  
Vol 80 (12) ◽  
pp. 1991-1997 ◽  
Author(s):  
Michael Tesmer ◽  
Shannon Wallet ◽  
Theofilos Koutouzis ◽  
Tord Lundgren
Keyword(s):  
Dental Implant ◽  
Bacterial Colonization ◽  
In Vitro Study ◽  
Vitro Study
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