scholarly journals Clinical and radiographic evaluation of platelet-rich fibrin as an adjunct to bone grafting demineralized freeze-dried bone allograft in intrabony defects

2020 ◽  
Vol 24 (1) ◽  
pp. 60 ◽  
Author(s):  
Abhinav Atchuta ◽  
JagadishReddy Gooty ◽  
VikramReddy Guntakandla ◽  
SunilKumar Palakuru ◽  
Satyanarayana Durvasula ◽  
...  
Keyword(s):  
Bone Grafting ◽  
Bone Allograft ◽  
Radiographic Evaluation ◽  
Intrabony Defects ◽  
Platelet Rich Fibrin ◽  
Freeze Dried

scholarly journals Clinical and radiographic evaluation of demineralized freeze-dried bone allograft with concentrated growth factor versus concentrated growth factor alone in the treatment of intrabony defects

2021 ◽  
Vol 94 (2) ◽  
pp. 220-228
Author(s):  
Tithi Vaid ◽  
Santosh Kumar ◽  
Rupal Mehta ◽  
Sujay Shah ◽  
Surabhi Joshi ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Bone Allograft ◽  
T Test ◽  
Radiographic Evaluation ◽  
Control Group ◽  
Intrabony Defects ◽  
Freeze Dried ◽  
Intergroup Comparison ◽  
And Control

Background. Periodontal disease is one of the major causes of alveolar bone loss. There are various ways of regenerating the lost bone, i.e. guided tissue regeneration, bone grafts, and growth factors. In this purview, it becomes immensely important for a clinician to decide the best modality of treatment. In this study, we compared the effect of demineralized freeze-dried bone allograft (DFDBA) in combination with concentrated growth factors (CGF) verses CGF alone. Methods. This double-blind, split-mouth study was conducted on ten patients with two comparable bilateral intrabony defects. Each pair of defects was randomly treated by DFDBA + CGF or CGF alone. Clinical parameters such as plaque index (PI), modified gingival index (MGI), pocket probing depth (PPD), and relative attachment level (RAL) were recorded at baseline, three months, and six months. In addition, radiograph with grids was also taken at baseline and six months. The paired t-test was used to compare the pre- and post-treatment values and the unpaired t-test was used to compare the test and control group. Results. The PI score decreased significantly from baseline to six months. Similarly, the mean MGI score decreased significantly from baseline to six months. The intragroup comparison showed that there was a significant reduction in PPD in both the test and control group. However, the intergroup comparison showed that the reduced pocket depth was not significant. The intragroup radiographic comparison showed that there was the significant formation of bone in both the test and control group but inter-group showed that the formation of bone among both the group were non-significant. Conclusion. Radiographic and clinical outcomes of this study concluded that post six months, both groups demonstrated significant improvement in clinical and radiographic parameters. However, the addition of DFDBA to CGFs did not give any additional benefits.

Evaluation of Human Periodontal Ligament and Osteosarcoma Cell Attachment and Viability on Particulate Bone and Dentin Allografts

2020 ◽  
pp. 1-6
Keyword(s):  
Particle Size ◽  
Cell Lines ◽  
Bone Grafting ◽  
Periodontal Ligament ◽  
Cell Attachment ◽  
Bone Allograft ◽  
Osteosarcoma Cell ◽  
Cellular Attachment ◽  
Freeze Dried ◽  
Demineralized Dentin

Purpose: Various bone grafting substitutes have been used in the periodontics for bone regeneration which include autografts, allografts, xenografts and alloplasts. Autogenous particulate dentin has been used successfully as a bone grafting substitute. The aim of present study was to evaluate the effect of demineralized and mineralized freeze-dried bone allograft and particulate dentin on osteoblasts-like cells and human periodontal fibroblasts. Materials and methods: Demineralized freeze-dried bone allograft (DFDBA) and freeze-dried bone allograft (FDBA) and ground dentin was used in the study. Particulate dentin was divided into four groups according to the size of the particles and demineralization - small dentin (particle size less than 200 µm), small dentin demineralized, large dentin (particle size 250-1200 µm), large dentin demineralized. Effect of all the specimens was checked on osteoblast-like cells (MG63) and human periodontal ligament cell lines. Percentage of surviving cells was measured using colorimetric MTT assay spectrophotometrically on 7th and 14th day of the cell culture. Scanning electron microscopy (SEM) was used to check the cellular attachment. Results: Demineralized dentin matrix has shown significantly enhanced viable cell percentage for both the cell lines. DFDBA and demineralized dentin has reported comparable percentage of surviving cells. Dentin seems to be more compatible with osteoblastslike cells than fibroblast. FDBA has shown the least favorable results. Cellular attachment for both the cell lines can be appreciated on SEM images. Conclusion: Demineralized particulate dentin has reported considerable percentage of cell viability making it a reasonable option for bone grafting substitute.

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