scholarly journals Evaluation and association of periodontal status with levels of Porphyromonas gingivalis in chronic periodontitis with and without Type 2 diabetes mellitus following nonsurgical periodontal therapy using quantitative polymerase chain reaction: An interventional study

2021 ◽  
Vol 25 (5) ◽  
pp. 399
Author(s):  
AbhayPandurang Kolte ◽  
PranitaAvinash Rode ◽  
RajashriAbhay Kolte ◽  
HemantJyotiswarup Purohit ◽  
RenukaKashi Swami
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Polymerase Chain Reaction ◽  
Type 2 Diabetes Mellitus ◽  
Quantitative Polymerase Chain Reaction ◽  
Interventional Study ◽  
Chain Reaction ◽  
Nonsurgical Periodontal Therapy ◽  
Polymerase Chain

scholarly journals Association of polymorphisms of genes TCF7L2, FABP2, KCNQ1, ADIPOQ with the prognosis of the development of type 2 diabetes mellitus

2020 ◽  
Vol 92 (10) ◽  
pp. 40-47
Author(s):  
E. S. Mel’nikova ◽  
O. D. Rymar ◽  
A. A. Ivanova ◽  
S. V. Mustafina ◽  
M. Ju. Shapkina ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Polymerase Chain Reaction ◽  
Type 2 Diabetes Mellitus ◽  
Control Group ◽  
Chain Reaction ◽  
Men And Women ◽  
Polymerase Chain ◽  
Diabetes Mellitus 2

Aim.To study the possibility of using polymorphisms of genesTCF7L2,FABP2,KCNQ1,ADIPOQas markers for predicting the development of type 2 diabetes mellitus (T2D) in the population of Novosibirsk. Materials and methods.On the basis of prospective observation of a representative population sample of residents of Novosibirsk (HAPIEE), 2 groups were formed according to the case-control principle (case people who had diabetes mellitus 2 over 10 years of observation, and control people who did not developed disorders of carbohydrate metabolism). T2D group (n=443, mean age 56.26.7 years, men 29.6%, women 70.4%), control group (n=532, mean age 56.17.1 years, men 32.7%, women 67.3%). DNA was isolated by phenol-chloroform extraction. Genotyping was performed by the method of polymerase chain reaction with subsequent analysis of restriction fragment length polymorphism, polymerase chain reaction in real time. Statistical processing was carried out using the SPSS 16.0 software package. Results and discussion.No significant effect of rs1799883 of theFABP2gene, rs2237892 of theKCNQ1gene, and rs6773957 of theADIPOQgene on the risk of developing T2D was found. Genotypes TT and TC rs7903146 of theTCF7L2gene are genotypes for the risk of developing T2D (relative risk RR 3.90, 95% confidence interval CI 2.316.61,p0.001; RR 1.86, 95% CI 1.422.43,p0.001, respectively). The CC genotype rs7903146 of theTCF7L2gene is associated with a protective effect against T2D (RR 0.37, 95% CI 0.290.49,p0.001). When theTCF7L2gene is included in the model for assessing the risk of developing T2D rs7903146, it retains its significance in both men and women. Conclusion.The rs7903146 polymorphism of theTCF7L2gene confirmed its association with the prognosis of the development of T2D, which indicates the possibility of considering it as a candidate for inclusion in a diabetes risk meter. Variants of risk meters have been developed to assess the prognosis of the development of diabetes mellitus 2 in men and women aged 4569 years during 10 years of follow-up. The association with the prognosis of the development of T2D polymorphisms rs1799883 of theFABP2gene, rs2237892 of theKCNQ1gene and rs6773957 of theADIPOQgene was not found.

scholarly journals The association of TCF7L2 rs7903146 polymorphism with type 2 diabetes mellitus among Tatars of Bashkortostan

2015 ◽  
Vol 19 (2) ◽  
pp. 119-124
Author(s):  
Diana S. Avzaletdinova ◽  
Leisan F. Sharipova ◽  
Olga V. Kochetova ◽  
Tatyana Vyacheslavovna Morugova ◽  
Vera V. Erdman ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Clinical Symptoms ◽  
Positive Association ◽  
Lipid Levels ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tcf7l2 Rs7903146

Aim. To perform the analysis of the association of transcription factor 7-like 2 (TCF7L2) gene rs7903146 polymorphism with type 2 diabetes mellitus (T2DM) among Tatars of Bashkortostan. Materials and methods. In this study, 169 patients with T2DM and 286 controls without clinical symptoms and laboratory signs of diabetes and without diabetes relatives were examined. Amplification of the DNA fragments was performed using real-time polymerase chain reaction (PCR) and TaqMan technique. Results. Genotype CT and allele T ratios were higher in the T2DM group than in controls (46. 7% vs. 36. 4%, p = 0. 030; 41. 7% vs. 30. 8%, p = 0. 001 respectively). There was a positive association between allele T and T2DM (OR = 1. 61), and allele C had a protective effect (OR = 0. 62, p = 0,001). Carriers of the ТТ genotype had later onset of T2DM (mean = 59. 5 years old) compared with carriers of the CT and CC genotypes (56. 1 years old, p = 0. 044). Basal C-peptide concentration, lipid levels and body mass index were not associated with TCF7L2 rs7903146 polymorphism. Conclusion. TCF7L2 rs7903146 polymorphism is associated with T2DM among Tatars of Bashkortostan. 

Impact of Endothelial NOS VNTR variant on suspectibility to diabetic neuropathy and type 2 diabetes mellitus

2020 ◽  
Vol 17 ◽  
Author(s):  
Serbulent Yigit ◽  
Ayse Feyda Nursal ◽  
Suheyla Uzun ◽  
Husniye Rustemoglu ◽  
Payam Amiri Dashatan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Enos Gene ◽  
Chain Reaction ◽  
Homozygous Genotype ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Increased Susceptibility

Purpose:: The aim of this study was to evaluate whether the VNTR intron 4b/4a variant in the eNOS gene is associated with type 2 diabetes mellitus (T2DM) and DPN. Methods:: A total of 598 subjects were enrolled into the study. eNOS VNTR 4b/4a variant was genotyped by polymerase chain reaction (PCR) method. Results:: eNOS VNTR intron 4b/4b genotype and b allele increased in patients with both DPN and T2DM compared healthy controls (p=0.0005, OR:1.94, p= 0.000002, OR:4.10, respectively). 4a/4b genotype was more prevalent in controls than in DPN and T2DM patients (p=0.00008, OR:0.46; p=0.000004, OR:0.24, respectively). eNOS VNTR b allele was more common in DPN patients and T2DM patients compared with controls (p=0.007, p=0.00002, respectively). Conclusion:: The eNOS VNTR “4b/4b” homozygous genotype and hence "4b"allele as genetic risk factor for T2DM and DPN, which may serve as a useful marker of increased susceptibility to the risk of these disorders.

scholarly journals Medication adherence and clinical outcomes in type 2 diabetes mellitus patients with depression: a prospective interventional study

2018 ◽  
Vol 22 (1) ◽  
pp. 269-276
Author(s):  
Veintramuthu Sankar ◽  
Antony Justin ◽  
Payman Hosseiny Matin ◽  
Swathy Pradeep ◽  
Nattam Sai Charan Kumar
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Medication Adherence ◽  
Clinical Outcomes ◽  
Interventional Study

scholarly journals Association Study between BGLAP Gene HindIII Polymorphism and Type 2 Diabetes Mellitus Development in Ukrainian Population

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Yaroslav D. Chumachenko ◽  
Viktoriia Yu. Harbuzova ◽  
Alexander V. Ataman
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Single Nucleotide Polymorphism ◽  
Type 2 Diabetes Mellitus ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Hindiii Polymorphism ◽  
Polymerase Chain

Type 2 diabetes mellitus (T2DM) belongs to the diseases with hereditary predisposition, so both environmental and genetic factors contribute to its development. Recent studies have demonstrated that the skeleton realizes systemic regulation of energy metabolism through the secretion of osteocalcin (OCN). Thus, the association analysis between HindIII single nucleotide polymorphism of OCN gene (BGLAP) promoter region and T2DM development in Ukrainian population was carried out. 153 individuals diagnosed with T2DM and 311 control individuals were enrolled in the study. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The lack of association between BGLAP HindIII single nucleotide polymorphism (SNP) and T2DM development among Ukrainians was found. Further studies with extended groups of comparison are needed to confirm the obtained results.

scholarly journals Hyperuricemia prevalence in Indian subjects with underlying comorbidities of hypertension and/or type 2 diabetes: a retrospective study from subjects attending hyperuricemia screening camps

2020 ◽  
Vol 8 (3) ◽  
pp. 794
Author(s):  
Himanshu Patel ◽  
Dhiren Shah
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Medical Records ◽  
Disease Duration ◽  
Interventional Study ◽  
High Prevalence ◽  
Increasing Trend ◽  
Study Participants

Background: To determine the prevalence of HU in Indian subjects attending the HU screening camps and in subjects with type 2 Diabetes Mellitus (T2DM), Hypertension (HTN), and T2DM+HTN.Methods: This was a retrospective, non-interventional study where medical records of subjects attending HU screening camps across 592 locations in India, between June 2017 to May 2018, were analyzed.Results: A total of 197097 subjects (T2DM: 19.69%; HTN: 14.08%; T2DM+HTN: 21.60%) attended the screening camps. Mean age of the study participants was 48.43±13.38 years (Male: 53.80%). A total of 48606(24.66%) subjects had HU. In the overall population, a higher proportion of subjects with T2DM + HTN (7.36%) had HU in comparison to subjects with T2DM (5.63%) and HTN (4.25%) alone. Similar results were reported when the data was evaluated only in HU subjects (T2DM+HTN: 29.85%; T2DM: 22.82%; HTN: 17.22%). Proportion of HU subjects increased with age, with the maximum prevalence evident in subjects aged >50 years (12.94%), followed by 30-50 years (10.65%) and <30 years (1.07%). Gender-wise, a slightly higher proportion of males (14.13%) were found to be hyperuricemic than females (10.53%). Higher proportion of subjects with disease (T2DM and/or HTN) duration of 2-5 years were found to be hyperuricemic in comparison with subjects with disease duration of >5 years or <2 years. Similar results were reported when the data was evaluated in the overall HU subjects and by indication.Conclusions: Authors observed a high prevalence of HU among subjects attending HU camps and those with associated comorbidities. The prevalence of HU was higher in males and has an increasing trend with age. Furthermore, the prevalence of HU was observed to be higher in subjects with 2-5 years of duration of T2DM and/or HTN.

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