scholarly journals Assessment of cell viability in four novel endodontic sealers

2018 ◽  
Vol 12 (02) ◽  
pp. 287-291 ◽  
Author(s):  
Vassiliki Taraslia ◽  
Ema Anastasiadou ◽  
Christina Lignou ◽  
Georgios Keratiotis ◽  
Anastasia Agrafioti ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Statistical Analysis ◽  
Cell Viability ◽  
Periodontal Ligament ◽  
Biological Properties ◽  
Viable Cells ◽  
Pdl Cells ◽  
Ah Plus ◽  
Level Of Significance ◽  
Endodontic Sealers

ABSTRACT Objective: The aim of this study was to evaluate the viability of human periodontal ligament (PDL) cells on MTA-Fillapex, GuttaFlow 2, TotalFill Sealer, and BioRootTM RCS in comparison to conventional epoxy resin-based (AH Plus) and zinc-oxide-eugenol-based (Roth’s 801) sealers. Materials and Methods: Sealers were divided into two groups, and five coverslips for each material per group were prepared. In the first group, PDLs were added immediately after the preparation of sealers (Fresh Group), and in the second, PDLs were added after 24 h. PDLs were cultured for 72 h and afterward, counted using standard hematocytometry. A Mann–Whitney U-test and Kruskal–Wallis test were used for the statistical analysis. The level of significance was set at 5%. Furthermore, cell morphology was assessed by confocal microscopy. Results: The number of viable cells for the 24 h-set groups was higher than the freshly mixed in all sealers except Roth’s 801. In both groups, GuttaFlow 2 presented the highest number of viable cells. In a descending order of cells’ survival, TotalFill, BioRoot, and MTA-Fillapex are following and the conventional sealers, AH Plus and Roth’s 801, seem not to exhibit the biological properties of the others. Cells grown on GuttaFlow 2, TotalFill, and BioRoot were observed to be well-formed. In contrast, MTA-Fillapex exhibited untypical morphology. No cells were detected on the surfaces of AH Plus, as well as Roth’s 801. Conclusions: All novel sealers presented increased cell viability in comparison to conventional sealers. GuttaFlow 2 exhibited the highest cell viability.

scholarly journals Assessment of platelet-rich fibrin in the maintenance and recovery of cell viability of the periodontal ligament

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lorena Bortolini Navarro ◽  
Fabiane Barchiki ◽  
Wilson Navarro Junior ◽  
Everdan Carneiro ◽  
Ulisses Xavier da Silva Neto ◽  
...  
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Trypan Blue ◽  
Enzymatic Digestion ◽  
Type Ii ◽  
Platelet Rich Fibrin ◽  
Pdl Cells ◽  
Autologous Platelet ◽  
Number Of Cells ◽  
Group 1

AbstractThis study analyzed the efficacy of autologous platelet-rich fibrin (PRF) in maintaining and recovering cell viability of the periodontal ligament (PDL). The PDL cells were isolated from 45 extracted teeth randomly distributed among 6 groups: 5 min, 1 h, 2 h, PRF 30 min, PRF 1 h and PRF 2 h. In the groups 5 min, 1 h and 2 h (n = 5), the teeth were kept dry in extra-alveolar times of 5 min, 1 h and 2 h respectively. The teeth of the groups PRF 30 min, PRF 1 h and PRF 2 h (n = 10) were kept dry at extra-alveolar times of 30 min, 1 and 2 h followed by immersion in PRF for 45 min. PDL cells were isolated by enzymatic digestion with type II collagenase and dispase, counted and analyzed for viability with Trypan blue vital dye in Neubauer chamber. The variables total number of cells and cell viability demonstrated that in the 5 min, 1 h and 2 h groups there was a decrease after the extra-alveolar dry times of 1 and 2 h. In comparison with the total number of cells, group 1 h, considered immediate reimplantation, did not present statistical difference when compared to the groups PRF 30 min, PRF 1 h and 2 h, a result that demonstrates that PRF assists in cell maintenance and recovery. PRF provided increased cell viability in relation to the different dry extra-alveolar times analyzed (p < 0.001). Autologous PRF presented effectiveness in maintaining and recovering PDL cells from extracted teeth and kept dry for up to 2 h.

scholarly journals In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

2020 ◽  
Vol 10 (1) ◽  
pp. 325 ◽  
Author(s):  
Francisco Javier Rodríguez-Lozano ◽  
Sergio López-García ◽  
David García-Bernal ◽  
Miguel R. Pecci-Lloret ◽  
Julia Guerrero-Gironés ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Periodontal Ligament ◽  
Biological Properties ◽  
Periodontal Ligament Stem Cells ◽  
Bioactive Materials ◽  
Tukey Test ◽  
Vitro Effect

New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (p < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (p < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material.

scholarly journals Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

Materials ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3087 ◽  
Author(s):  
Sergio López-García ◽  
Miguel R. Pecci-Lloret ◽  
Julia Guerrero-Gironés ◽  
María P. Pecci-Lloret ◽  
Adrián Lozano ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Viability ◽  
Epoxy Resin ◽  
Cell Attachment ◽  
Pairwise Comparison ◽  
Periodontal Ligament Stem Cells ◽  
Matrix Mineralization ◽  
Mineralization Potential ◽  
Ah Plus ◽  
Endodontic Sealers

The aim of this study was to investigate the cytocompatibility and mineralization potential of two premixed hydraulic endodontic sealers compared with an epoxy resin-based root canal sealer. The cellular responses and mineralization capacity were studied in human periodontal ligament stem cells (hPDLSCs) that were exposed to premixed hydraulic sealers, Bio-C Sealer (Angelus, Londrína, PR, Brazil), TotalFill BC Sealer (FKG Dentaire SA, La-Chaux-de-fonds, Switzerland) and an epoxy resin-based material, AH Plus (Dentsply De Trey, Konstanz, Germany). Non-exposed cultures served as the control. The endodontic sealers were assessed using scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). Statistical analyses were done using Analisis of Variance (ANOVA), with Bonferroni adjusted pairwise comparison (p = 0.05). AH Plus reduced cell viability and cell migration, whereas increased cell viability and cell migration were observed in the Bio-C Sealer and the TotalFill BC Sealer (p < 0.05). The lowest cell attachment and spreading were observed for all concentrations of AH Plus, whereas the highest were observed for TotalFill BC Sealer. At the end of 21 days, only the Bio-C Sealer and the TotalFill BC Sealer supported matrix mineralization (p < 0.05). Additionally, SEM-EDX revealed high content of calcium, oxygen, and silicon in the Bio-C Sealer and the TotalFill BC Sealer. Based on the results from this study, Bio-C Sealer and TotalFill BC Sealer demonstrated better cytocompatibility in terms of cell viability, migration, cell morphology, cell attachment, and mineralization capacity than AH Plus.

Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells

2016 ◽  
Vol 35 (9) ◽  
pp. 983-990 ◽  
Author(s):  
Xin Ge ◽  
Ying-Feng Liu ◽  
Yong Wong ◽  
Li-Zheng Wu ◽  
Ling Tan ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Viability ◽  
Periodontal Ligament ◽  
Tobacco Consumption ◽  
Periodontal Ligament Cells ◽  
Nicotine Treatment ◽  
Pdl Cells ◽  
Induced Apoptosis ◽  
The Impact

Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones. CD4+ T cell-mediated immune response is involved in the progression of periodontitis. Tobacco consumption increases the risk of periodontal disease. However, the impact of nicotine on the interaction between human periodontal ligament (PDL) cells and CD4+ T cells remains unrevealed. Our study aims to investigate the effect of nicotine on PDL cells and the cocultured CD4+ T cells. The PDL cell cultures were established by explants from healthy individuals, exposed to nicotine or α-bungarotoxin (α-BTX), and incubated solely or in combination with CD4+ T cells. Afterwards, cell viability, secreted cytokines, and matrix metalloproteinases (MMPs) were evaluated. In monoculture of PDL cells, nicotine dramatically repressed cell viability and increased apoptosis. Meanwhile, α-BTX largely reversed the nicotine-induced apoptosis and increased viability of PDL cells. Compared with the monoculture, MMP-1, MMP-3, interleukin (IL)-1β, IL-6, IL-17, and IL-21 in supernatant of cocultures were markedly elevated after treatment with nicotine. Moreover, α-BTX significantly attenuated nicotine-triggered production of these components either in mono- or co-cultures. In addition, PDL cell-derived CXCL12 following nicotine treatment recruited CD4+ T cells. Above all, nicotine deteriorated periodontitis partially by promoting PDL cell–CD4+ T cell-mediated inflammatory response and matrix degradation.

scholarly journals Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

Materials ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5242
Author(s):  
Hanseul Oh ◽  
Egan Kim ◽  
Sukjoon Lee ◽  
Soyeon Park ◽  
Dongzi Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Cell Viability ◽  
Epoxy Resin ◽  
Calcium Silicate ◽  
Periodontal Ligament ◽  
Expression Level ◽  
Osteogenic Potential ◽  
Periodontal Ligament Stem Cells ◽  
Ah Plus

The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.

scholarly journals Evaluation of Manual and Two-Rotary Niti Retreatment Systems in Removing Gutta-Percha Obturated with Two Root Canal Sealers

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Athikesavan Jayasenthil ◽  
Emmanuel Solomon Sathish ◽  
Prashanth Prakash
Keyword(s):  
Zinc Oxide ◽  
Statistical Analysis ◽  
Study Design ◽  
Root Canal ◽  
Filling Material ◽  
Canal Wall ◽  
Gutta Percha ◽  
Ah Plus ◽  
Root Canal Sealers ◽  
One Way Anova

Objective. The objective of this study was to evaluate the efficacy of two retreatment NiTi systems (protaper universal retreatment files, R-Endo), when compared to manual technique in removing Gutta-percha obturated with two sealers. Study Design. Sixty extracted single-rooted premolars were instrumented with Protaper rotary files till F3. The specimens were divided into six groups. Groups 1, 2, 3 were obturated with Gutta-percha and zinc oxide eugenol and Groups 4, 5, 6 were obturated with Gutta-percha and AH-plus. The retreatment was carried out in groups 1 and 4 with H-files and GGdrills, groups 2 and 5 with R-endo retreatment files and groups 3 and 6 with Protaper retreatment files. The roots were sectioned and evaluated under optical stereomicroscope. Statistical analysis was performed with one-way ANOVA and Newman-Keul's test at . Results. The manual technique resulted in cleaner canal walls when compared with both rotary retreatment systems. Conclusion. NiTi rotary retreatment files can be used to remove the filling material quickly, but it should be followed by hand instruments to obtain better canal wall cleanliness.

Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells

2008 ◽  
Vol 9 (6) ◽  
pp. 17-24 ◽  
Author(s):  
Fatih Ozan ◽  
Zübeyde Akin Polat ◽  
Bektaş Tepe ◽  
Kürşat Er
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Tap Water ◽  
Salvia Officinalis ◽  
The Other ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Trypan Blue Exclusion ◽  
Storage Media ◽  
Pdl Cells

Aim The purpose of this study was to determine the ability of Salvia officinalis (S. officinalis) extracts to serve as a storage medium for the maintenance of periodontal ligament (PDL) cell viability of avulsed teeth. Methods and Materials PDL cells were obtained from healthy third molars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultures were subjected to 4, 2.5, 1.5, and 0.5% S. officinalis solutions, Hank's balanced salt solution (HBSS), phosphate buffered saline (PBS), and tap water. Tissue culture plates were incubated with experimental media at 37°C for 1, 3, 6, 12 or 24 hours. PDL cell viability was assessed by trypan blue exclusion. Statistical analysis of the data was performed by one-way analysis of variance (ANOVA) complemented by the Tukey's test. The level of significance was 5% (p< 0.05). Results The results showed 2.5% S. officinalis was a more effective storage medium than the other experimental solutions (p<0.05). Only at 1 hour and 3 hours was there found similar effect between 2.5% S. officinalis and HBSS. At 24 hours, 2.5% S. officinalis was found to be significantly better than the other solutions tested. Conclusion S. officinalis can be recommended as a suitable transport medium for avulsed teeth. Clinical Significance The findings of this study support the use of S. officinalis as another option for clinicians to use to store and transport avulsed teeth until reimplantation procedures can be done. Citation Özan F, Polat ZA, Tepe B, Er K. Influence of Storage Media Containing Salvia officinalis on Survival of Periodontal Ligament Cells. J Contemp Dent Pract 2008 September; (9)6:017-024.

Evaluation of Aloevera Gel as a Storage Medium in Maintaining the Viability of Periodontal Ligament Cells - An in Vitro Study

2016 ◽  
Vol 40 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Punit Fulzele ◽  
Sudhindra Baliga ◽  
Nilima Thosar ◽  
Debaprya Pradhan
Keyword(s):  
Drinking Water ◽  
Periodontal Ligament ◽  
In Vitro Study ◽  
Periodontal Ligament Cells ◽  
Blue Dye ◽  
Storage Medium ◽  
Viable Cells ◽  
Pdl Cells ◽  
Significant Difference

Objective: To investigate the effectiveness of aloevera gel as a new storage medium in maintaining the viability of periodontal ligament cells. Study design: Premolars extracted for orthodontic reason were obtained. Confluent monolayers of fibroblasts were grown by cell culture method from the PDL cells isolated from the extracted teeth. One ml of this cell suspension was transferred to wells of culture plates, incubated for 24 hrs, followed by exposure to the three experimental media, Hank's balanced salt solution (HBSS), aloevera gel, and packaged drinking water. These plates were then assessed for viable cells using trypan blue dye exclusion test with haemocytometer after 15, 30, 60, 90 and 120 mins. The results obtained were statistically analysed using one-way analysis of variance (ANOVA). Results: At 15 min, HBSS presented maximum mean percentage of viable PDL cells (89%), followed by aloevera at 81% and packaged drinking water at 10%. Aloevera demonstrated 71%, 59%, 57% viable cells at 30, 60, 90 mins respectively. At 120 min, HBSS presented 57% viable cells followed by aloevera gel (45%) and packaged drinking water (3%). No statistical significant difference was observed between HBSS and aloevera gel. Conclusions: Within the parameters of this study, both aloevera gel and HBSS were effective in maintaining the viability of PDL cells. Hence, aloevera gel could be used as a storage media for avulsed tooth in situations where availability of HBSS is in question.

Viability and Reproducibility of Periodontal Ligament Cells on Avulsed Teeth Stored in Ham's F-10 Solution

2018 ◽  
Vol 42 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Maryam Talebi ◽  
Iman Parisay ◽  
Jalil Tavakol afshari ◽  
Arezoo Shajiei ◽  
Mostafa Sofiani Ghadim
Keyword(s):  
Cell Viability ◽  
Mtt Assay ◽  
Periodontal Ligament ◽  
Tap Water ◽  
Skim Milk ◽  
Periodontal Ligament Cells ◽  
Pdl Cells ◽  
Experimental Media ◽  
Significant Difference ◽  
Better Than

Objectives: The purpose of the present study was to evaluate the efficacy of Ham's F-10 in maintaining the viability and reproducibility of PDL cells on avulsed teeth. Study design: Sixty mature, healthy extracted premolars were used. The experimental media used were Ham's F-10, Hank's balanced salt solution (HBSS), skim milk, and tap water (n = 15 specimens each). Cell viability was tested after 1, 3, 6, and 24 h storage in medium. Cell reproducibility was assessed by methyl-thiazol-tetrazolium (MTT) assay after1, 3, and 6 h storage in Ham's F-10, HBSS, and tap water. Results: The viability of PDL cells stored in Ham's F-10 and HBSS was significantly greater than that of samples stored in milk and tap water at all-time points (P&lt;0.001). A significant difference in cell viability between samples stored in Ham's F-10 and HBSS (favoring the former) was observed only at 6h (P=0.04). MTT assay results were significantly better for samples stored in Ham's F-10 and HBSS than for those stored in tap water (P&lt;0.001), with a significant difference between Ham's F-10 and HBSS observed only at 3h (P&lt;0.001). Conclusions: Ham's F-10 is capable of preserving PDL cells viable and reproducible better than milk and tap water and similar to HBSS.

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