scholarly journals Evaluation of anti-plaque microbial activity of Azadirachta indica (neem oil) in vitro: A pilot study

2012 ◽  
Vol 4 (6) ◽  
pp. 394 ◽  
Author(s):  
K Vennila ◽  
KB Naziya ◽  
Sugumari Elavarasu ◽  
P Abinaya ◽  
S Elanchezhiyan ◽  
...  
Keyword(s):  
Pilot Study ◽  
Microbial Activity ◽  
Azadirachta Indica ◽  
Neem Oil

scholarly journals Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok

2001 ◽  
Vol 44 (4) ◽  
pp. 419-423 ◽  
Author(s):  
Edson Hirose ◽  
Pedro M. O. J. Neves ◽  
João A. C. Zequi ◽  
Luís H. Martins ◽  
Cristiane H. Peralta ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Azadirachta Indica ◽  
Organic Agriculture ◽  
Metarhizium Anisopliae ◽  
Neem Oil ◽  
Colony Diameter ◽  
Negative Effect ◽  
Fungitoxic Effect

The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion <FONT FACE=Symbol>Ô</FONT> and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibion<FONT FACE=Symbol>Ô</FONT> caused major inhibition on M. anisopliae, with reductions in germination (-37.74%), colony diameter (-30.26%) and conidiogenesis (-42.62%). Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%), colony diameter (-36.62%) and conidiogenesis (-84.93%).

Anti-microbial activity of a new vaginal contraceptive NIM-76 from neem oil (Azadirachta indica)

2000 ◽  
Vol 71 (3) ◽  
pp. 377-382 ◽  
Author(s):  
M SaiRam ◽  
G Ilavazhagan ◽  
S.K Sharma ◽  
S.A Dhanraj ◽  
B Suresh ◽  
...  
Keyword(s):  
Microbial Activity ◽  
Azadirachta Indica ◽  
Neem Oil

scholarly journals Effet des huiles de pourghère (Jatropha curcas L.) et de neem (Azadirachta indica

2020 ◽  
Vol 14 (4) ◽  
pp. 1282-1293
Author(s):  
Issa Adamou ◽  
Zakari Abdoul Habou ◽  
Moussa S. Hassimi ◽  
Salamatou M. Mamane ◽  
Toudou Adam
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Azadirachta Indica ◽  
Jatropha Curcas ◽  
Rhizopus Oryzae ◽  
Inhibition Zone ◽  
Curvularia Lunata ◽  
Neem Oil

Les huiles de Jatropha curcas communément appelé pourghère et de neem (Azadirachta indica A. Juss), sont testées comme alternatives aux pesticides chimiques dans l’optique d’y déceler de potentiels effets fongicides tout en préservant l’environnement. Les effets des deux huiles végétales sont comparés à ceux de deux fongicides conventionnels, la griséofulvine 250 mg et la nystatine ainsi que l’alcool 95°, sur cinq champignons phytopathogènes (Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum et Rhizopus oryzae). Les observations ont porté sur la mesure du diamètre de la zone d’inhibition exercée par les différentes substances inhibitrices à travers les milieux gélosés. Le dispositif expérimental appliqué a été celui du bloc complètement randomisé de Fisher avec huit répétitions. Les moyennes des diamètres de la zone d’inhibition sont respectivement de 26,51 mm pour la nystatine, 14,53 mm pour la griséofulvine, 4,53 mm pour l’huile de neem, 3,05 mm pour l’huile de jatropha, et 0,00 mm pour l’alcool 95°. L’huile de neem a eu un effet inhibiteur net sur la croissance de l’ensemble des champignons testés. En revanche, l’huile de jatropha a exercé un meilleur contrôle de la croissance des espèces Aspergillus flavus, Aspergillus niger et Rhizopus oryzae. Les deux huiles pourraient être recommandées dans la protection des denrées stockées, notamment les céréales.Mots clés : Extraits végétaux, effet fongicide, zone d’inhibition, lutte alternative. English Title: Effect of Jatropha (Jatropha curcas L.) and neem (Azadirachta indica A. Juss) oils on in vitro devloppement of pathogenic funji Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum et Rhizopus oryzae Oils of Jatropha curcas (pourghère) and neem (Azadirachta indica A. Juss) were tested as alternatives to chemical pesticides in a view to finding some potential fungicide effects harmless to environment. The effects of the two natural oils were compared with those of two conventional fungicides, griseofulvin 250 mg and nystatin and also alcohol 95° on five phytopathogenic fungi (Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum and Rhizopus oryzae). Observations consisted in measuring the diameter of the zone of inhibition by different substances through the agarose medium. The experimental device applied was the Fisher completely randomized bloc with eight repetitions. Means of inhibition zone diameters were respectively de 26.51 mm for nystatin, 14.53 mm for griseofulvin, 4.53 mm for neem oil, 3.05 mm for jatropha oil, and 0.00 mm for alcohol 95°. The neem oil showed good inhibition effect on the growth of the whole set of tested fungi. In contrast, jatropha oil exhibited a better fungal growth control on Aspergillus flavus, Aspergillus niger and Rhizopus oryzae species. Thus, these two vegetable oils could be recommended in cereal protection in postharvest.Keywords: Vegetal oils, fungicide effects, inhibition zone, alternative control.

scholarly journals In Vitro Activity of Neem (Azadirachta indica) Oil on Growth and Ochratoxin A Production by Aspergillus carbonarius Isolates

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 579 ◽  
Author(s):  
Mariana Paiva Rodrigues ◽  
Andrea Luciana Astoreca ◽  
Águida Aparecida de Oliveira ◽  
Lauranne Alves Salvato ◽  
Gabriela Lago Biscoto ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Ochratoxin A ◽  
Azadirachta Indica ◽  
Mycelial Growth ◽  
Aspergillus Carbonarius ◽  
In Vitro Activity ◽  
Neem Oil ◽  
In Vitro Antifungal Activity

Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.

scholarly journals In Vitro Effectiveness of Neem Oil (Azadirachta Indica A. Juss) Shampoo as Anti Head Lice (Pediculus humanus capitis)

2019 ◽  
Vol 4 (3) ◽  
pp. 76
Author(s):  
Norisca A. Putriana ◽  
Azizul Hakim ◽  
Patihul Husni ◽  
Taofik Rusdiana
Keyword(s):  
Azadirachta Indica ◽  
Corneal Opacity ◽  
Head Lice ◽  
Neem Oil ◽  
Pediculus Humanus Capitis ◽  
Eye Irritation ◽  
Pediculus Humanus ◽  
Study Results ◽  
Irritation Test

Head lice (Pediculus humanus capitis), a parasite causing hair problem,  often occurs in society.The study aimed to evaluate in vitroeffectiveness of shampoo formulation containing neem oil as an anti head lice. An experimental method was used to examine the formulation of shampoo, irritation test, testing the effectiveness of shampoo against head lice (Pediculicidal activity), and testing the effectiveness of shampoo against nits (Ovicidal activity). The effectiveness test employed an in vitromethod where neem oil shampoo were tested directly towards head lices and nits isolated from humans, then the mortality was observed. To recognize the safety of the shampoo, skin and eye irritation tests were carried out by applying 0.5 ml on the skin and dripping 0.1 ml shampoo into New Zealand white rabbit’s eyes.Data were analyzed using Kruskal Wallis and Mann Whitney. The study results showed that the primary irritation index for 5%, 10%, 15%, and 20% neem oil shampoo were 0; 0111; 0.222; and 0.222, respectively. All of the shampoos were very mild irritation response category. Eye irritation tests showed that only the 20% neem oil shampoo gave a mild irritation response with degree of corneal opacity ≥ 1 and recovered after 7 days of observation. The 5%, 10%, 15%, and 20% neem oil shampoo were effective as an anti head lice. The 15% and 20% neem oil shampoo had anti head lice effectiveness activity as same as 1% permethrin lotion. In conclusion, the 15% neem oil shampoo was chosen as the best shampoo, its high anti head lice effect and light irritation response. Keywords: Head lice, neem oil (Azadirachta indica A. Juss), shampoo

The preparation of neem oil microemulsion (Azadirachta indica) and the comparison of acaricidal time between neem oil microemulsion and other formulations in vitro

2010 ◽  
Vol 169 (3-4) ◽  
pp. 399-403 ◽  
Author(s):  
Jiao Xu ◽  
Qiao-Jia Fan ◽  
Zhong-Qiong Yin ◽  
Xu-Ting Li ◽  
Yong-Hua Du ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Neem Oil

Pilot study of antimicrobic and anti-biofilm activity of a combination of Ciprofloxacin and Tinidazolum in vitro

2019 ◽  
Vol 24 (4) ◽  
pp. 285-292
Author(s):  
V. N. Tsarev ◽  
R. V. Ushakov ◽  
E. V. Ippolitov ◽  
M. S. Podporin ◽  
N. N. Nuruev
Keyword(s):  
Pilot Study

Effectiveness of Neem (Azadirachta indica A. Juss) Oil against Decay Fungi

2017 ◽  
Vol 5 (1) ◽  
pp. 48-51 ◽  
Author(s):  
Kanchan Rawat ◽  
◽  
Uttam Kumar Sahoo ◽  
Nagaraj Hegde ◽  
Awadhesh Kumar ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Schizophyllum Commune ◽  
Antimicrobial Properties ◽  
Fusarium Proliferatum ◽  
Wood Preservatives ◽  
Neem Oil ◽  
Environment Friendly ◽  
Decay Fungi ◽  
Natural Oils ◽  
Mycelia Growth

The enormous use of metallic wood preservatives has caused destructive impact on environment as well as human health. Therefore realizing the urgency of switching to Environment friendly options such as natural oils are being tested for their antimicrobial properties. The present study aimed at investigating potential of Neem oil against the growth ofdecaying fungi. The ability of Neem oil to inhibit mycelia growth of Schizophyllum commune, Fusarium oxysporum, Fusarium proliferatum, Coniophora puteana and Alternaria alternata was tested at different concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, 4.0, 6.0, 8.0 and 10%. Results of the study revealed Neem oil concentrations above 2% were significantly inhibitory to all the tested fungi.

The Safety and Antimicrobial Properties of Stabilized Hypochlorous Acid in Acetic Acid Buffer for the Treatment of Acute Wounds—a Human Pilot Study and In Vitro Data

2021 ◽  
pp. 153473462110156
Author(s):  
Ewa A. Burian ◽  
Lubna Sabah ◽  
Klaus Kirketerp-Møller ◽  
Elin Ibstedt ◽  
Magnus M. Fazli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Hypochlorous Acid ◽  
Treatment Time ◽  
Donor Site ◽  
Antimicrobial Properties ◽  
Prolonged Treatment ◽  
Wound Irrigation ◽  
The Mean ◽  
And Performance

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. The study enrolled 12 subjects scheduled for a split-skin graft transplantation, where the donor site was used as a model of an acute wound. The treatment time was 75 s, given on 6 occasions. A total of 7 adverse events were regarded as related to the treatment; all registered as pain during the procedure for 2 subjects. One subject had a wound infection at the donor site. The mean colony-forming unit (CFU) decreased by 41% after the treatment, and the mean epithelialization was 96% on both days 14 (standard deviation [SD] 8%) and 21 (SD 10%). The study provides preliminary support for the safety, well-tolerance, and efficacy of HOCl + buffer for acute wounds. The pain was frequent although resolved quickly. Excellent wound healing and satisfying antimicrobial properties were observed. A subsequent in vitro biofilm study also indicated good antimicrobial activity against Pseudomonas aeruginosa with a 96% mean reduction of CFU, when used for a treatment duration of 15 min ( P < .0001), and a 50% decrease for Staphylococcus aureus ( P = .1010). Future larger studies are needed to evaluate the safety and performance of HOCl + buffer in acute wounds, including the promising antimicrobial effect by prolonged treatment on bacterial biofilms.

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