scholarly journals In vitro activity of neem oil [Azadirachta indica A. Juss (Meliaceae)] on Aspergillus flavus growth, sporulation, viability of spores, morphology and Aflatoxins B1 and B2 production

2010 ◽  
Vol 01 (04) ◽  
pp. 292-299 ◽  
Author(s):  
Christiane L. da Costa ◽  
Marcia R. F. Geraldo ◽  
Carla C. Arrotéia ◽  
Carlos Kemmelmeier
Keyword(s):  
Aspergillus Flavus ◽  
Azadirachta Indica ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Neem Oil

scholarly journals Effet des huiles de pourghère (Jatropha curcas L.) et de neem (Azadirachta indica

2020 ◽  
Vol 14 (4) ◽  
pp. 1282-1293
Author(s):  
Issa Adamou ◽  
Zakari Abdoul Habou ◽  
Moussa S. Hassimi ◽  
Salamatou M. Mamane ◽  
Toudou Adam
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Azadirachta Indica ◽  
Jatropha Curcas ◽  
Rhizopus Oryzae ◽  
Inhibition Zone ◽  
Curvularia Lunata ◽  
Neem Oil

Les huiles de Jatropha curcas communément appelé pourghère et de neem (Azadirachta indica A. Juss), sont testées comme alternatives aux pesticides chimiques dans l’optique d’y déceler de potentiels effets fongicides tout en préservant l’environnement. Les effets des deux huiles végétales sont comparés à ceux de deux fongicides conventionnels, la griséofulvine 250 mg et la nystatine ainsi que l’alcool 95°, sur cinq champignons phytopathogènes (Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum et Rhizopus oryzae). Les observations ont porté sur la mesure du diamètre de la zone d’inhibition exercée par les différentes substances inhibitrices à travers les milieux gélosés. Le dispositif expérimental appliqué a été celui du bloc complètement randomisé de Fisher avec huit répétitions. Les moyennes des diamètres de la zone d’inhibition sont respectivement de 26,51 mm pour la nystatine, 14,53 mm pour la griséofulvine, 4,53 mm pour l’huile de neem, 3,05 mm pour l’huile de jatropha, et 0,00 mm pour l’alcool 95°. L’huile de neem a eu un effet inhibiteur net sur la croissance de l’ensemble des champignons testés. En revanche, l’huile de jatropha a exercé un meilleur contrôle de la croissance des espèces Aspergillus flavus, Aspergillus niger et Rhizopus oryzae. Les deux huiles pourraient être recommandées dans la protection des denrées stockées, notamment les céréales.Mots clés : Extraits végétaux, effet fongicide, zone d’inhibition, lutte alternative. English Title: Effect of Jatropha (Jatropha curcas L.) and neem (Azadirachta indica A. Juss) oils on in vitro devloppement of pathogenic funji Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum et Rhizopus oryzae Oils of Jatropha curcas (pourghère) and neem (Azadirachta indica A. Juss) were tested as alternatives to chemical pesticides in a view to finding some potential fungicide effects harmless to environment. The effects of the two natural oils were compared with those of two conventional fungicides, griseofulvin 250 mg and nystatin and also alcohol 95° on five phytopathogenic fungi (Aspergillus flavus, Aspergillus niger, Curvularia lunata, Fusarium oxysporum and Rhizopus oryzae). Observations consisted in measuring the diameter of the zone of inhibition by different substances through the agarose medium. The experimental device applied was the Fisher completely randomized bloc with eight repetitions. Means of inhibition zone diameters were respectively de 26.51 mm for nystatin, 14.53 mm for griseofulvin, 4.53 mm for neem oil, 3.05 mm for jatropha oil, and 0.00 mm for alcohol 95°. The neem oil showed good inhibition effect on the growth of the whole set of tested fungi. In contrast, jatropha oil exhibited a better fungal growth control on Aspergillus flavus, Aspergillus niger and Rhizopus oryzae species. Thus, these two vegetable oils could be recommended in cereal protection in postharvest.Keywords: Vegetal oils, fungicide effects, inhibition zone, alternative control.

scholarly journals In Vitro Activity of Neem (Azadirachta indica) Oil on Growth and Ochratoxin A Production by Aspergillus carbonarius Isolates

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 579 ◽  
Author(s):  
Mariana Paiva Rodrigues ◽  
Andrea Luciana Astoreca ◽  
Águida Aparecida de Oliveira ◽  
Lauranne Alves Salvato ◽  
Gabriela Lago Biscoto ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Ochratoxin A ◽  
Azadirachta Indica ◽  
Mycelial Growth ◽  
Aspergillus Carbonarius ◽  
In Vitro Activity ◽  
Neem Oil ◽  
In Vitro Antifungal Activity

Aspergillus carbonarius is a saprobic filamentous fungus, food spoiling fungus and a producer of ochratoxin A (OTA) mycotoxin. In this study, the in vitro antifungal activity of neem oil (0.12% p/p of azadirachtin) was evaluated against the growth of six strains of A. carbonarius and the production of OTA. Four different concentrations of neem oil were tested in addition to three incubation times. Only the concentration of 0.3% of neem oil inhibited more than 95% of the strain’s growth (97.6% ± 0.5%), while the use of 0.5% and 1.0% of neem oil showed lower antifungal activity, 40.2% ± 3.1 and 64.7% ± 1.1, respectively. There was a complete inhibition of OTA production with 0.1% and 0.3% neem oil in the four strains isolated in the laboratory from grapes. The present study shows that neem essential oil can be further evaluated as an auxiliary method for the reduction of mycelial growth and OTA production.

The high efficacy of luliconazole against environmental and otomycosis Aspergillus flavus strains

2020 ◽  
Author(s):  
Maryam Moslem ◽  
Ali Zarei Mahmoudabadi
Keyword(s):  
Amphotericin B ◽  
Aspergillus Flavus ◽  
Inhibitory Concentration ◽  
Topical Therapy ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Environmental Isolates ◽  
Strong Activity ◽  
Microscopic Features

Background and Objectives: Luliconazole is currently confirmed for the topical therapy of dermatophytosis. Moreover, it is found that luliconazole has in vitro activity against some molds and yeast species. The aim of the present study was to evaluate the efficacy of luliconazole in comparison to routine used antifungals on clinical and environmental isolates of Aspergillus flavus. Materials and Methods: Thirty eight isolates of A. flavus (18 environmental and 20 clinical isolates) were detected based on morphological and microscopic features and also PCR-sequencing of β-tubulin ribosomal DNA gene. All the isolates were tested against luliconazole, voriconazole, amphotericin B and caspofungin. Minimum inhibitory concentration (MIC),   90   MIC50, MIC isolates.   and MIC Geometric (GM) were calculated using CLSI M38-A2 protocol for both environmental and clinical   GM   Results: Luliconazole with extremely low MIC range, 0.00049-0.00781 μg/mL and MIC   0.00288 μg/mL showed very   strong activity against both clinical and environmental A. flavus isolates. Moreover, voriconazole inhibited 100% of isolates at defined epidemiological cutoff values (ECV ≤ 2 µg/ml). 50% and 27.8% of clinical and environmental isolates of A. flavus, were resistant to caspofungin, respectively. Whereas, all the isolates were found to be resistant to amphotericin B.   GM   Conclusion: The analysis of our data clearly indicated that luliconazole (with MIC   0.00244 µg/ml for clinical and 0.00336   μg/ml for environmental isolates) had the highest in vitro activity against A. flavus strains.

In Vitro Activity of Phytocannabinoids from High Potency Cannabis sativa

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
A Husni ◽  
S Ross ◽  
O Dale ◽  
C Gemelli ◽  
G Ma ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
High Potency
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