scholarly journals PREVALENCE OF HEPATITIS C AMONG EGYPTIAN CHILDREN WITH SICKLE CELL DISEASE AND THE ROLE OF IL28B GENE POLYMORPHISMS IN SPONTANEOUS VIRAL CLEARANCE

2016 ◽  
Vol 8 ◽  
pp. 2016007 ◽  
Author(s):  
Somaia Mohammed Mousa ◽  
Mona Kamal El-Ghamrawy ◽  
Mona Kamal El-Ghamrawy ◽  
Heba Gouda ◽  
Heba Gouda ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Gene Polymorphisms ◽  
Hcv Rna ◽  
Spontaneous Clearance ◽  
Cell Disease ◽  
Persistent Viremia ◽  
Egyptian Children ◽  
Hcv Antibody

Background and objectives: Hepatitis C virus (HCV) is a major health problem in Egypt with its prevalence estimated to be 14.7% among general population in 2008. Patients receiving frequent blood transfusions like sickle cell disease (SCD) are more exposed to the risk of acquiring HCV. IL28B gene polymorphisms have been associated with spontaneous HCV clearance. This study aims to determine the prevalence of HCV infection among children with SCD and to study the relation between IL28B gene polymorphisms and spontaneous HCV clearance.Methods: Seventy SCD patients were screened for HCV antibody. HCV positive patients were tested for the level of HCV RNA using quantitative real time PCR. IL28B polymorphisms (rs 12979860 SNP and rs 12980275 SNP) were detected using Taqman QRT-PCR and sequence specific primers PCR respectively. Results: Sixteen patients (23%) were HCV antibody positive, 9 of them (56.3%) had undetectable HCV RNA in serum and 7 (43.7%) had persistent viremia. Genotypes CC/CT/TT of rs12979860 were found in 30 (42.9%), 29 (41.4%) and 11 (15.7%) and rs12980275 AA/AG/GG were found in 8 (11.4%), 59 (84.3%) and 3 (4.3%).  There was no significant difference in the frequency of IL28B (rs 12979860 and rs12980275) genotypes among HCV patients who cleared the virus and those with persistent viremia (p=0.308 and 0.724 respectively). Conclusion: Egyptian SCD patients have high prevalence of HCV. Muti-transfused patients still exposed to a risk of transmission of HCV. IL28B gene polymorphisms are not associated with spontaneous clearance of HCV in this cohort of Egyptian children with SCD.

scholarly journals PREVALENCE OF HEPATITIS B, HEPATITIS C AND HIV IN MULTIPLY TRANSFUSED SICKLE CELL DISEASE PATIENTS FROM OMAN

2019 ◽  
Vol 11 (1) ◽  
pp. e2019058
Author(s):  
Anil Pathare ◽  
Salam Alkindi
Keyword(s):  
Hepatitis C ◽  
Sickle Cell Disease ◽  
Hepatitis B ◽  
Sickle Cell ◽  
Hepatitis B Surface Antigen ◽  
Normal Liver ◽  
Hcv Rna ◽  
Cell Disease ◽  
Positive Serology ◽  
Cross Sectional

Background: In Oman, the prevalence of hepatitis B (HBV) infection is 5.8% with 2.8–7.1% HBV carriers. Hepatitis C (HCV) prevalence amongst Omanis is 0.41%. A total of 2917 human immunodeficiency virus (HIV) infections were notified amongst Omanis by 2017. This study was performed as there was no data on the prevalence of HIV, HBV and HCV in sickle cell disease (SCD) patients from Oman.   Study Design and Methods: In this retrospective, cross-sectional study, medical records of all SCD patients who attended our hospital between 2011 to 2017 were retrieved from the hospital information system. Following approval by the local medical research and ethics committee, data on HIV, HBV and HCV exposure were recorded to estimate the prevalence.   Results: Amongst a total of 1000 SCD patients (491 males and 509 females), twenty-three (2.3%) patients showed positive serology for hepatitis B surface antigen (HbsAg), of whom sixteen (1.6%) were HBV DNA positive.  126 (12.6%) had anti-HCV antibodies (anti-HCV), of whom fifty-two (5.2%) were HCV RNA positive. None of the patients had positive serology for HIV. A normal liver was observed on abdominal ultrasound in 788 (78.8%) patients, whereas, 208 (20.8%) had hepatomegaly and 4 (0.4%) had liver cirrhosis. Thirty-six (3.6%) patients died, but in only two patients, the mortality was due to cirrhosis of the liver.   Conclusion: This study provides the first comprehensive data on the prevalence of HBV and HCV infections among Omani SCD patients exposed to blood transfusions. Reassuringly, no case with HIV was observed.   Keywords: Prevalence; Hepatitis; HBV; HCV; HIV; infection  

Treatment of chronic hepatitis C in sickle cell disease and thalassaemic patients with interferon and ribavirin

2009 ◽  
Vol 21 (7) ◽  
pp. 726-729 ◽  
Author(s):  
David Ancel ◽  
Xavier Amiot ◽  
Dominique Chaslin-Ferbus ◽  
Isabelle Hagege ◽  
Armand Garioud ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Sickle Cell Disease ◽  
Chronic Hepatitis C ◽  
Sickle Cell ◽  
Cell Disease

scholarly journals Klotho gene polymorphisms and their association with sickle cell disease phenotypes

2015 ◽  
Vol 37 (4) ◽  
pp. 275-276 ◽  
Author(s):  
Claudia R. Lustosa Souza ◽  
Marily M. Azevedo Shimmoto ◽  
Perla Vicari ◽  
Grazielle Mecabo ◽  
Martha Mariana Arruda ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Gene Polymorphisms ◽  
Cell Disease ◽  
Disease Phenotypes ◽  
Klotho Gene

Prevalence of factor V Leiden (G1619A) and prothrombin gene (G20210A) mutation in Egyptian children with sickle cell disease

2012 ◽  
Vol 22 (4) ◽  
pp. 697-702
Author(s):  
Mona Salah El-Din Hamdy ◽  
Heba Mahmoud Gouda ◽  
Iman Abdel-Mohsen Shaheen ◽  
Mervat M. Khorshied ◽  
Rania Hosny Tomerak
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Factor V Leiden ◽  
Factor V ◽  
Cell Disease ◽  
G20210a Mutation ◽  
Egyptian Children ◽  
Prothrombin Gene

Acute Painful Crises of Sickle Cell Disease in Egyptian Children: Predictors of Severity for a Preventive Strategy

2006 ◽  
Vol 83 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Mohammad Al-Haggar ◽  
Hala Al-Marsafawy ◽  
Nabeel Abdel-Razek ◽  
Rizk Al-Baz ◽  
Abdel-Hamid Mostafa
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Preventive Strategy ◽  
Cell Disease ◽  
Egyptian Children

scholarly journals Alpha-1 Antitrypsin and SERPINA1 gene Mutation As New Biomarker in Sickle Cell Disease

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 4918-4918
Author(s):  
Magda Oliveira Seixas Carvalho ◽  
Carvalho Luís André ◽  
Mauri­cio Batista Carvalho ◽  
Larissa Carneiro Rocha ◽  
Valma Maria Lopes Nascimento ◽  
...  
Keyword(s):  
Steady State ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Blood Cells ◽  
Gene Polymorphisms ◽  
Clinical History ◽  
Control Group ◽  
Cell Disease ◽  
Serpina1 Gene ◽  
Alpha 1 Antitrypsin

Abstract Sickle cell disease (SCD) has heterogeneous clinical picture and there are several pathway involved in SCD pathogenesis, and chronic inflammation and hemolysis are important hallmarks of the disease. Based on such points, there is a search for prognostic markers to establish possible sub-genotypes of the disease. The present study investigated the alpha-1 antitrypsin (AAT) levels, a serum glycoprotein inhibitor of proteases responsible for trigger inflammatory reactions, describing its associations with SERPINA1 gene polymorphisms, hematological and chemistry biomarkers, and clinical history in a group of SCD children in steady-state. The study was approved at FIOCRUZ research board and followed the principle of Declaration of Helsinki. A total of 356 steady state unrelated SCD patients were included at the present study and a control group (CG) compound by 100 unrelated healthy individuals sex and age matched with the patients group, which were from the same geographical origin. Patients age ranging 13.96+9.91 years of age, the AAT levels higher than the 50th percentile (158.0 mg/mL) had significantly lower red blood cells (RBC) count (p=0.003), hemoglobin (Hb) (p=0.0002) and hematocrit (Hct) (0.0002) concentration, and higher white blood cells (WBC) (p=0.004) and neutrophils (p=0.0001) counts, and higher C-reactive protein (CRP) levels (p<0.0001), and lower urea levels (p=0.0003). AAT had significant negative correlation with RBC (r=-0.205, p=0.0001), Hb (r=-0.203, p=0.0001), Hct (r=-0.256, p<0.0001), high density lipoprotein cholesterol (HDL-C) (r=-0.205, p=0.0001), urea (r=-0.184, p=0.0005), creatinine (r=-134, p=0.012), and albumin (r=-0.135, p=0.011), and significant positive correlation with WBC (r=0.18, 0.0007), neutrophils (r=0.2297, p<0.0001), HbS (r=0.2874), total bilirubin (r=0.137, p=0.01), direct bilirubin (r=0.136, p=0.001), indirect bilirubin (r=0.115, p=0.032), lactate dehydrogenase (r=0.159, p=0.003), ferritin (r=0.1353, 9=0.011), CRP (r=0.355, p<0.0001). Patients with higher levels of AAT had clinical history of more than three infection episodes (OR=1.71, CI:1.05-2.65, p=0.02), gallstones presence (OR=1.75, CI:1.03-2.97, p=0.02), and received more blood therapy (OR=2.35, CI:1.51-3.65, p=0.0001).The SERPINA1 gene polymorphisms were analyzed in 126 SCD patients and 100 CG individuals, the protease inhibitor (Pi)*MM or wide type genotype was find in 115 (91.3%) of SCD patients and 92 (92%) of CG; the PI*SS genotype was find in 2 (1.6%) SCD patients and in none individual of the CG; the PI*MS genotype was find in 9 (7.1%) SCD patients and in 6 (6%) CG individuals; and the PI*MZ genotype was find in 2 (2%) CG individuals. Ours results suggest that the AAT may be considerer as a marker of SCD patients’ outcome, once increased levels is related to stress situation, anti-inflammatory and inhibitory proteases properties and may act as a physiological and cytoprotective regulator influencing the severity of SCD. Our results related to SERPINA1 genotypes emphasize the role of the mutant allele in decrease the AAT production what may represent a risk factor. Additional studies should be carried out in order to investigate mechanisms throughout the AAT can induce several important events in the SCD pathogenesis. Disclosures No relevant conflicts of interest to declare.

scholarly journals Evaluation of Alpha-1 Antitrypsin Levels and SERPINA1 Gene Polymorphisms in Sickle Cell Disease

2017 ◽  
Vol 8 ◽  
Author(s):  
Magda Oliveira Seixas Carvalho ◽  
André Luís Carvalho Santos Souza ◽  
Mauricio Batista Carvalho ◽  
Ana Paula Almeida Souza Pacheco ◽  
Larissa Carneiro Rocha ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Gene Polymorphisms ◽  
Cell Disease ◽  
Serpina1 Gene ◽  
Alpha 1 Antitrypsin

scholarly journals Iron overload parameters and early detection of cardiac disease among Egyptian children and young adults with β-thalassaemia major and sickle cell disease: a cross-sectional study

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 1108
Author(s):  
Khaled Salama ◽  
Amina Abdelsalam ◽  
Hadeel Seif Eldin ◽  
Eman Youness ◽  
Yasmeen Selim ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Pulmonary Artery ◽  
Iron Overload ◽  
Sickle Cell ◽  
Systolic Pulmonary Artery Pressure ◽  
Thalassaemia Major ◽  
Cell Disease ◽  
Cardiac Iron ◽  
Diastolic Velocity ◽  
Egyptian Children

Background: Cardiac, hepatic and pancreatic T2* measured by magnetic resonance imaging (MRI) has been proven to be an accurate and non-invasive method for measuring iron overload in iron overload conditions. There is accumulating evidence that pancreatic iron can predict cardiac iron in young children because the pancreas loads earlier than the heart. The aim of our study was to investigate cardiac function and cardiac iron and their relation to pancreatic iron among patients with β-thalassaemia major (βTM) and sickle cell disease (SCD). Methods: 40 βTM and 20 transfusion-dependant SCD patients were included along with 60 healthy age-matched controls. Echocardiography and Tissue Doppler Imaging were performed for all subjects as well as the control group.  Hepatic, cardiac and pancreatic iron overload in cases were assessed by MRI T2*. Results: The study group consisted of 40 βTM and 20 transfusion dependant SCD patients with mean age 13.7 years and mean frequency of transfusion/year 12. Mean cardiac T2* was 32.9 ms and mean myocardial iron concentration was 0.7 mg/g; One patient had cardiac iron overload of moderate severity. Mean pancreatic T2* was 22.3 ms with 20 patients having mild pancreatic iron overload. Pancreatic T2* correlated positively with main pulmonary artery diameter (p=0.046), peak late diastolic velocity at septal mitral annulus (p=0.038), peak early diastolic velocity at tricuspid annulus (p=0.001) and mitral annular plane systolic excursion (p=0.01); and negatively with end systolic pulmonary artery pressure (p=0.007). We couldn’t test the predictability of pancreatic T2* in relation to cardiac T2* as only one patient had cardiac T2*<20 ms. Conclusion: Assessment of pancreatic T2* in multi-transfused patients with βTM and SCD can predict myocardial dysfunction. No direct relation between pancreatic iron and cardiac siderosis was detected.

Ledipasvir/Sofosbuvir for Hepatitis C Cirrhosis With Sickle Cell Disease and Thalassemia Minor

2017 ◽  
Vol 112 ◽  
pp. S1262-S1263
Author(s):  
Abhishek D. Polavarapu ◽  
Hassan Al Moussawi ◽  
Mayurathan Kesavan ◽  
Stephen Mulrooney
Keyword(s):  
Hepatitis C ◽  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Cell Disease ◽  
Thalassemia Minor

l-selectin gene polymorphisms and complications of sickle cell disease

2008 ◽  
Vol 30 (4) ◽  
pp. 312-316 ◽  
Author(s):  
C. C. UGOCHUKWU ◽  
I. OKPALA ◽  
P. PANTELIDIS ◽  
B. INUSA ◽  
O. IBEGBULAM ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Sickle Cell ◽  
Gene Polymorphisms ◽  
Cell Disease
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