scholarly journals Technique for intraoperatory harvesting of adipose derived stem cells: towards cell treatment of recalcitrant ulcers

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Ilaria Zollino ◽  
Maria Grazia Sibilla ◽  
Sergio Gianesini ◽  
Erica Menegatti ◽  
Mirko Tessari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Alternative Treatment ◽  
Treatment Options ◽  
Technical Note ◽  
Adipose Derived Stem Cells ◽  
Novel Therapy

Successful wound and ulcer repair remains a major biomedical challenge in the 21<sup>st</sup> Century. Innovative and alternative treatment options have been investigated over the last decade and stem cells application has been suggested as a possible novel therapy for regenerative medicine. In particular, stem cells derived from adipose tissue have been attracting a lot of attention in recent years as an alternative to the use of cells derived from bone marrow. This technical note describes the procedure introduced by Coleman for intraoperatory harvesting of adipose derived-stem-cells and proposes a rationale for using it in difficult wound healing and recalcitrant ulcers.

scholarly journals COMPARISON OF PROLIFERATION AND IMMUNOPHENOTYPE OF MSC, OBTAINEDFROM BONE MARROW, ADIPOSE TISSUE AND UMBILICAL CORD

2015 ◽  
Vol 7 (2) ◽  
pp. 14-22
Author(s):  
A A Aisenstadt ◽  
N I Enukashvili ◽  
T L Zolina ◽  
L V Alexandrov ◽  
A B Smoljaninov
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Clinical Practice ◽  
Umbilical Cord ◽  
Adipose Derived Stem Cells ◽  
Non Invasive ◽  
Bone Marrow Adipose Tissue ◽  
Proliferation Capacity ◽  
Marrow Adipose Tissue

Mesenchymal stem cells (MSC) can be applied for treatment of different diseases. Human MSC have been isolated from bone marrow, adipose tissue, umbilical cord blood. umbilical cord MSCs (uC- MSC) are obtained during birth with non-invasive, non-traumatic methods and thus seem a good candidate for clinical practice instead of bone-marrow MSC (BM-MSC). It is yet unknown wheth- er the immunophenotype and proliferation capacity of uC-MSC are similar to adipose-derived stem cells (AdSCs) or BM-MSC. The goal of this research was to study the immunophenotype and prolifer- atiion capacity of uC-MSC, AdSCs and BM-MSC. The results indicated that MSC of different origin had similar morphological and immunophenotypic characteristics with minor differences. uC-MSC differed from other cultures by constant level of Cd105 expression, the presence of minor Cd10 - and Cd13 - populations and higher proliferative activity. BM-MSC were characterized by reduced expres- sion levels of Cd90, compared with the uC-MSCs and AdSCs. These data confirm the similarity of uC-MSC with BM-MSCs and AdSCs and the possibility of their use in clinical practice instead of hard-to-obtain BM-MSC.

scholarly journals Adipose-derived stem cells for tissue engineering and therapy of non-healing wounds

2018 ◽  
Vol 72 ◽  
pp. 806-821 ◽  
Author(s):  
Adriana Schumacher ◽  
Mirosława Cichorek ◽  
Michał Pikuła
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Clinical Trials ◽  
Adipose Tissue ◽  
Growth Factors ◽  
Chronic Wounds ◽  
Clinical Effects ◽  
Adipose Derived Stem Cells ◽  
Healing Wounds

Chronic wounds seem to be a big problem for the medicinal, social and commercial area, especially for elder patients or people with cancer, metabolic or autoimmune diseases. In this respect, in the modern regenerative medicine there are intensive studies on methodologies that stimulate healing of chronic wounds (diabetic foots, ulcers, burns). In tissue engineering new solutions in wound healing are based on cellular therapies which consisting of growth factors and various types of scaffolds. In this way, there are created skin substitutes which are composed of cellular auto/allografts (stem cells and differentiated cells) and most commonly biodegradable scaffolds; they aim is not only to fill the tissue but also to stimulate wound healing. In this article we demonstrate the current knowledge about biological properties of Adipose- -derived Stem Cells (ASCs), methods of their isolation and potential for use in therapies for non-healing wounds. Adipose tissue seems to be an attractive and abundant stem cells source with therapeutic applicability in diverse phase of the repair and regeneration of the chronically damaged tissues. Additionally, it is believed that secreted by ASCs growth factors, cytokines and exosomes are decisive in the clinical effects. In this review, we also present the current clinical trials using stem cells derived from adipose tissue. Increasingly, the use of cell therapy in wound healing treatment draws attention to the safety, reproducibility and quality of stem cells. Researches go on and therapy approaches are possible but the detailed knowledge of the ASCs biology must be thoroughly investigated before these cells would be widely used in the clinical trials.

scholarly journals From Human Mesenchymal Stem Cells to Insulin-Producing Cells: Comparison between Bone Marrow- and Adipose Tissue-Derived Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Mahmoud M. Gabr ◽  
Mahmoud M. Zakaria ◽  
Ayman F. Refaie ◽  
Engy A. Abdel-Rahman ◽  
Asmaa M. Reda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
C Peptide ◽  
Metabolic Characteristics ◽  
Insulin Producing Cells ◽  
Glucose Challenge

The aim of this study is to compare human bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs), for their differentiation potentials to form insulin-producing cells. BM-MSCs were obtained during elective orthotopic surgery and AT-MSCs from fatty aspirates during elective cosmetics procedures. Following their expansion, cells were characterized by phenotyping, trilineage differentiation ability, and basal gene expression of pluripotency genes and for their metabolic characteristics. Cells were differentiated according to a Trichostatin-A based protocol. The differentiated cells were evaluated by immunocytochemistry staining for insulin and c-peptide. In addition the expression of relevant pancreatic endocrine genes was determined. The release of insulin and c-peptide in response to a glucose challenge was also quantitated. There were some differences in basal gene expression and metabolic characteristics. After differentiation the proportion of the resulting insulin-producing cells (IPCs), was comparable among both cell sources. Again, there were no differences neither in the levels of gene expression nor in the amounts of insulin and c-peptide release as a function of glucose challenge. The properties, availability, and abundance of AT-MSCs render them well-suited for applications in regenerative medicine.Conclusion. BM-MSCs and AT-MSCs are comparable regarding their differential potential to form IPCs. The availability and properties of AT-MSCs render them well-suited for applications in regenerative medicine.

scholarly journals Expression of CD34 marker in the adipose tissue derived stem cells

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Phuc Van Pham ◽  
Ngoc Bich Vu ◽  
Van Hong Tran
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Hematopoietic Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
Hematopoietic Stem ◽  
Cd34 Expression

Introduction: Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marker of hematopoietic stem cells. This study aimed to analyze and determine the expression of CD34 marker in ten samples of ADSCs obtained from 10 donors. Methods: All ADSC samples were isolated and expanded according to the published previous protocols. They were confirmed as the MSCs with some markers and differentiation potential, excepting the CD34 expression. Then they were cultured and analyzed the expression of CD34 by flow cytometry at passage 3, 5, 7 and 9. Results: The results showed that expression of CD34 in ADSCs was different between donors and their passages that accounted from 1.21% to 23.38%. Conclusion: These results suggested that ADSCs are not ‘truly” MSCs like MSCs from bone marrow.

scholarly journals A Comprehensive Understanding of UA-ADRCs (Uncultured, Autologous, Fresh, Unmodified, Adipose Derived Regenerative Cells, Isolated at Point of Care) in Regenerative Medicine

2020 ◽  
Author(s):  
Eckhard Alt ◽  
Glenn Winnier ◽  
Alexander Haenel ◽  
Ralf Rothoerl ◽  
Oender Solakoglu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Point Of Care ◽  
Adipose Derived Stem Cells ◽  
Multipotent Stem Cells ◽  
Physiological Capacity ◽  
Regenerative Cells ◽  
Regenerate Tissue

It has become practically impossible to survey the literature on cells derived from adipose tissue with the aim to apply them in regenerative medicine. The aim of this review is to provide a jump start to understanding the potential of UA-ADRCs (uncultured, unmodified, fresh, autologous adipose derived regenerative cells isolated at the point of care) in regenerative medicine. We show that serious and adequate clinical research demonstrates that tissue regeneration with UA-ADRCs is safe and effective. ADRCs are neither 'fat stem cells' nor could they exclusively be isolated from adipose tissue, as ADRCs contain the same adult (depending on the definition) pluripotent or multipotent stem cells that are ubiquitously present in the walls of small blood vessels. Of note, the specific isolation procedure used has significant impact on the number and viability of the cells and hence on safety and efficacy of UA-ADRCs. Furthermore, there is no need to further separate adipose-derived stem cells (ASCs) from ADRCs if the latter were adequately isolated from adipose tissue. Most importantly, UA-ADRCs have the physiological capacity to adequately regenerate tissue without need for manipulating, stimulating and/or (genetically) reprogramming the cells for this purpose. Tissue regeneration with UA-ADRCs fulfills the criteria of homologous use.

The Use of Hoechst Staining To Identify Hematopoietic and Other Adult Stem Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4242-4242
Author(s):  
Rei Ogawa ◽  
Juri Fujimura ◽  
Hidemitsu Sugihara ◽  
Hidenori Suzuki ◽  
Hiko Hyakusoku ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Hematopoietic Stem Cells ◽  
Adult Stem Cells ◽  
Surface Antigens ◽  
Adipose Derived Stem Cells ◽  
Cell Surface Antigens ◽  
Hematopoietic Stem ◽  
Hoechst Staining

Abstract BACKGROUND: Hoechst staining has been used to identify hematopoietic stem cells, but it may also be useful in identifying other adult stem cells. Here we report our efforts to purify and characterize stem cells in the stromal vascular fraction of adipose tissue with Hoechst staining, also comparing our results with those of our studies using bone marrow. METHODS: Stromal vascular fractions (SVFs) of adipose tissue and whole bone marrow (BM) were harvested from C57BL/6N mice, as were stem cells. Then the cells were stained with Hoechst 33342 and analyzed by flow cytometry and the number of cells in the side population (SP) counted. Moreover, surface antigens of SP cells were analyzed by flow cytometry using antibodies against CD44, 45, 45R, Sca-1, and c-kit, respectively, for 30 min on ice. Finally, the morphologic characteristics of cells in the SP of both BM and SVF were observed using electron microscopy. RESULTS: The percentage of SP cells in BM was about 0.05 to 0.1% and that in the SVF was about 1.0 to 3.0%. The cell-surface antigens of BM expressed were CD44 (−), CD 45 (+), CD 45R (−), Sca-1 (+) and c-kit (+), while those of SVF were CD44 (−), CD 45 (−), CD 45R (+/−), Sca-1 (+/−) and c-kit (−). Upon electron microscopic observation, both BM and SVF cells in the SP were considered to be remarkably immature (immature cell organelles and a high N/C ratio). CONCLUSION: The rate and expression patterns of cell-surface antigens in SP cells derived from BM were consistent with the results of previous reports. However, the same characteristics in SP cells derived from SVFs were clearly different. At present it is not clear whether cells in the SP of SVFs are adipose-derived stem cells. Indications were that there are 10 to 60 times as many immature cells in adipose tissue as in bone marrow. Moreover, it is possible that the great majority of cells in the SP of SVFs are not hematopoietic stem cells but unique adipose-derived stem cells. Finally, our studies suggest that Hoechst staining may be useful for identifying not only hematopoietic stem cells but also other adult stem cells.

Evaluating the Effect of Integra Seeded with Adipose Tissue-Derived Stem Cells or Fibroblasts in Wound Healing

2020 ◽  
Vol 17 (7) ◽  
pp. 629-635
Author(s):  
Yuchang Wang ◽  
Quanrui Feng ◽  
Zhanfei Li ◽  
Xiangjun Bai ◽  
Yiping Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Skin Necrosis ◽  
Burn Patients ◽  
Adipose Derived Stem Cells ◽  
Bioengineered Skin

Background: Extensive loss of skin in burn patients can have devastating consequences, both physically and mentally. Adipose-Derived Stem Cells (ADSCs) and fibroblasts are known to play significant roles in the process of wound healing. Recently, bioengineered skin has been considered for wound healing purposes. Methods: Investigate the effect of Integra seeded with ADSCs, fibroblasts, or both on wound healing. Results: We found that when Integra is seeded with ADSCs and fibroblasts, both types of cells incorporate and proliferate, the phenomenon becoming more robust when the cells are co-cultured on Integra, both in vitro and in vivo. In addition, when these cells are seeded on Integra, they stimulate epithelization with no signs of inflammation and skin necrosis being observed when transplanted on animals for 7 days. Conclusion: ADSCs and fibroblasts seeded on Integra could decrease the number of α-SMA positive myofibroblasts, leading to scarless wound healing. The evidence from this study is strongly supportive that Integra seeded with ADSCs and fibroblasts is an appropriate and effective bioengineered skin for wound healing.

scholarly journals Stromal-vascular fraction of adipose tissue as an alternative source of cellular material for regenerative medicine

2021 ◽  
Vol 93 (1) ◽  
pp. 40-50
Author(s):  
A. S. Sultanova ◽  
◽  
O. Ya. Bespalova ◽  
O. Yu. Galkin ◽  
◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Clinical Medicine ◽  
Subcutaneous Fat ◽  
Stromal Vascular Fraction ◽  
Cellular Material ◽  
Adipose Derived Stem Cells ◽  
Alternative Source ◽  
Fat Deposits

Adipose tissue is the most convenient source of cellular material for regenerative medicine as it can be obtained in significant quantities via cosmetic liposuction, lipoaspiration of subcutaneous fat or by excision of fat deposits. Adipose tissue consists of adipocytes and cells, which are the part of the stromal-vascular fraction (SVF). Different cell populations can be isolated from SVF, among which the population of adipose tissue stem cells (adipose-derived stem cells, ADSC) is especially important for regenerative medicine. SVF can be obtained relatively easily from adipose tissue (adipose tissue is an alternative to bone marrow in terms of being a source of stem cells) and used to treat various pathologies. Recent studies show that SVF not only has a therapeutic effect similar to that of ADSC, but in some cases is even more effective. The article provides the analysis of the main methods of SVF obtainment, characteristics of SVF cellular composition, its potential for use in clinical medicine and its main advantages over other sources of cellular material, including­ ADSC cultured in vitro, for regenerative medicine. Keywords: adipocytes, adipose-derived stem cells, regenerative medicine, stromal-vascular fraction

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