scholarly journals From Human Mesenchymal Stem Cells to Insulin-Producing Cells: Comparison between Bone Marrow- and Adipose Tissue-Derived Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Mahmoud M. Gabr ◽  
Mahmoud M. Zakaria ◽  
Ayman F. Refaie ◽  
Engy A. Abdel-Rahman ◽  
Asmaa M. Reda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
C Peptide ◽  
Metabolic Characteristics ◽  
Insulin Producing Cells ◽  
Glucose Challenge

The aim of this study is to compare human bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs), for their differentiation potentials to form insulin-producing cells. BM-MSCs were obtained during elective orthotopic surgery and AT-MSCs from fatty aspirates during elective cosmetics procedures. Following their expansion, cells were characterized by phenotyping, trilineage differentiation ability, and basal gene expression of pluripotency genes and for their metabolic characteristics. Cells were differentiated according to a Trichostatin-A based protocol. The differentiated cells were evaluated by immunocytochemistry staining for insulin and c-peptide. In addition the expression of relevant pancreatic endocrine genes was determined. The release of insulin and c-peptide in response to a glucose challenge was also quantitated. There were some differences in basal gene expression and metabolic characteristics. After differentiation the proportion of the resulting insulin-producing cells (IPCs), was comparable among both cell sources. Again, there were no differences neither in the levels of gene expression nor in the amounts of insulin and c-peptide release as a function of glucose challenge. The properties, availability, and abundance of AT-MSCs render them well-suited for applications in regenerative medicine.Conclusion. BM-MSCs and AT-MSCs are comparable regarding their differential potential to form IPCs. The availability and properties of AT-MSCs render them well-suited for applications in regenerative medicine.

Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue

2019 ◽  
Vol 120 (7) ◽  
pp. 11842-11852 ◽  
Author(s):  
Simone Ortiz Moura Fideles ◽  
Adriana Cassia Ortiz ◽  
Amanda Freire Assis ◽  
Max Jordan Duarte ◽  
Fabiola Singaretti Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteoblast Differentiation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cell Source

scholarly journals Combined Transfection of the Three Transcriptional Factors, PDX-1, NeuroD1, and MafA, Causes Differentiation of Bone Marrow Mesenchymal Stem Cells into Insulin-Producing Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Guo Qing-Song ◽  
Zhu Ming-Yan ◽  
Wang Lei ◽  
Fan Xiang-Jun ◽  
Lu Yu-Hua ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Physiological Function ◽  
Insulin Biosynthesis ◽  
Insulin Producing Cells ◽  
Marrow Mesenchymal Stem Cells ◽  
Glucose Challenge ◽  
Streptozotocin Induced Diabetes ◽  
Treatment Of Diabetes

Aims. The goal of cell transcription for treatment of diabetes is to generate surrogateβ-cells from an appropriate cell line. However, the induced replacement cells have showed less physiological function in producing insulin compared with normalβ-cells.Methods. Here, we report a procedure for induction of insulin-producing cells (IPCs) from bone marrow murine mesenchymal stem cells (BM-mMSCs). These BM-mMSCs have the potential to differentiate into insulin-producing cells when a combination of PDX-1 (pancreatic and duodenal homeobox-1), NeuroD1 (neurogenic differentiation-1), and MafA (V-maf musculoaponeurotic fibrosarcoma oncogene homolog A) genes are transfected into them and expressed in these cells.Results. Insulin biosynthesis and secretion were induced in mMSCs into which these three genes have been transfected and expressed. The amount of induced insulin in the mMSCs which have been transfected with the three genes together is significantly higher than in those mMSCs that were only transfected with one or two of these three genes. Transplantation of the transfected cells into mice with streptozotocin-induced diabetes results in insulin expression and the reversal of the glucose challenge.Conclusions. These findings suggest major implications for cell replacement strategies in generation of surrogateβ-cells for the treatment of diabetes.

scholarly journals Deducing insulin producing cells in goat adipose tissue derived mesenchymal stem cells

2021 ◽  
Author(s):  
Amit Dubey ◽  
Hruda Nanda Malik ◽  
Dinesh Kumar ◽  
Sikander Saini ◽  
Satish Kumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Regenerative Medicine ◽  
Basic Mechanism ◽  
Specific Protein ◽  
Specific Gene ◽  
Pancreatic Cells ◽  
Insulin Producing Cells

Abstract Background: Mesenchymal stem cells (MSCs) is a new herald for regenerative medicine for control of incurable diseases in human and animals. Diabetes occurs when the blood glucose is high due to lack of insulin hormone secreted by the pancreatic cells. The global diabetes in 2019 is estimated 463 million people and rising to 578 million by 2030. Methods: Here we differentiated goat adipose tissue derived MSCs into insulin producing cells. To achieve this, the goat MSCs were cultured in serum-free DMEM/F12 medium containing glucose, nicotinamide, activin-A, exendin-4, pentagastrin, retinoic acid and mercaptoethanol for three weeks. The in vitro differentiation ADSCs into insulin-producing cells was confirmed by detecting the pancreatic endoderm specific markers i.e. Igf-1, Sst, Ngn3, Pdx-1, Isl-1, c-Kit, Thy-1, and Glut-2 in differentiating cells. Results: There was a significant increase in insulin specific gene expression with respect to duration of differentiation. Pancreatic insulin-producing cells were further characterized by immunolocalization of Pdx-1, insulin, and Islets-1 specific protein. The release of insulin in response to a glucose challenge was also evaluated. Conclusions: The study provides new opportunities for deciphering the basic mechanism of in vitro genesis of pancreatic cells and basic properties, availability, and abundance of ADSCs render them well-suited for applications in regenerative medicine.

Immunophenotype and gene expression profile of mesenchymal stem cells derived from canine adipose tissue and bone marrow

2014 ◽  
Vol 161 (1-2) ◽  
pp. 21-31 ◽  
Author(s):  
Rudell Screven ◽  
Elizabeth Kenyon ◽  
Michael J. Myers ◽  
Haile F. Yancy ◽  
Mark Skasko ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Gene Expression Profile ◽  
Expression Profile

scholarly journals Generation of Insulin-Producing Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells: Comparison of Three Differentiation Protocols

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Mahmoud M. Gabr ◽  
Mahmoud M. Zakaria ◽  
Ayman F. Refaie ◽  
Sherry M. Khater ◽  
Sylvia A. Ashamallah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Trichostatin A ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Diabetic Patients ◽  
Directed Differentiation ◽  
C Peptide ◽  
Insulin Producing Cells

Introduction. Many protocols were utilized for directed differentiation of mesenchymal stem cells (MSCs) to form insulin-producing cells (IPCs). We compared the relative efficiency of three differentiation protocols.Methods. Human bone marrow-derived MSCs (HBM-MSCs) were obtained from three insulin-dependent type 2 diabetic patients. Differentiation into IPCs was carried out by three protocols: conophylline-based (one-step protocol), trichostatin-A-based (two-step protocol), andβ-mercaptoethanol-based (three-step protocol). At the end of differentiation, cells were evaluated by immunolabeling for insulin production, expression of pancreatic endocrine genes, and release of insulin and c-peptide in response to increasing glucose concentrations.Results. By immunolabeling, the proportion of generated IPCs was modest (≃3%) in all the three protocols. All relevant pancreatic endocrine genes, insulin, glucagon, and somatostatin, were expressed. There was a stepwise increase in insulin and c-peptide release in response to glucose challenge, but the released amounts were low when compared with those of pancreatic islets.Conclusion. The yield of functional IPCs following directed differentiation of HBM-MSCs was modest and was comparable among the three tested protocols. Protocols for directed differentiation of MSCs need further optimization in order to be clinically meaningful. To this end, addition of an extracellular matrix and/or a suitable template should be attempted.

scholarly journals Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Catalysts ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 62
Author(s):  
Won-Yong Jeon ◽  
Seyoung Mun ◽  
Wei Beng Ng ◽  
Keunsoo Kang ◽  
Kyudong Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Cell Morphology ◽  
Electrical Current ◽  
Specific Gene ◽  
Next Generation ◽  
The Impact

Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices.

Sign in / Sign up

Export Citation Format

Share Document