scholarly journals Superior lines candidates evaluation of two local red rice Southeast Sulawesi cultivars (Indonesia) derived from gamma rays irradiation techniques

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ni Wayan Sri Suliartini ◽  
Kuswanto Kuswanto ◽  
Nur Basuki ◽  
Andy Soegianto
Keyword(s):  
Gamma Rays ◽  
First Generation ◽  
Upland Rice ◽  
Red Rice ◽  
Black Rice ◽  
Lodging Resistance ◽  
Ld50 Test

The aim of this research was to describe the potency of superior cultivar upland rice candidates (gogo rice) first generation (M1) which generated from Pae Pulu Pongasi and Pae Loilo, Southeast Sulawesi, Indonesia. The grains were irradiated by 60 Co gamma-rays at 100, 200, 300, and 400 Gy doses. The developed cultivar was selected from numerous red and black rice with highest anthocyanins. Based on LD50 test to these cultivars, <em>Pae Pulu Pongas</em>i cultivar was founded at 416 Gy and <em>Pae Loilo</em> about 360 Gy. Ten superior lines candidates were selected, namely M1.27K2D1, M1.190K2D3 which are derived from M1 Pae Pulu Pongasi cultivar and M1.6K3D1, M1.24K3D1, M1.37K3D1, M1.102K3D1, M1.104K3D1, M1.162K3D1, M1.170K3D1 which derived from M1 <em>Pae Loilo</em> cultivars. M1.81K2D1 derived from M1 <em>Pae Pulu Pongasi</em> had the potential to produce higher yield, high lodging resistance, and short harvest age compared to parental.

scholarly journals Advanced Yield Trial of F7 Upland Rice Lines with Lodging Resistance in Bangka Regency, Bangka Belitung Islands Province, Indonesia

2021 ◽  
Vol 926 (1) ◽  
pp. 012022
Author(s):  
E D Mustikarini ◽  
G I Prayoga ◽  
R Santi ◽  
N P E Sari
Keyword(s):  
Rice Plant ◽  
Upland Rice ◽  
Block Design ◽  
High Yield ◽  
Red Rice ◽  
Lodging Resistance ◽  
Randomized Block Design ◽  
Yield Trial ◽  
Experiment Method ◽  
Selection Of

Abstract Upland rice with a high yield becomes a priority in rice breeding activities. High yield can be obtained if the rice plant has lodging resistance. This study aims to determine the yield and selection of F7 upland rice lines as the candidate for superior varieties with lodging resistance. The research was conducted in ultisol land, Bangka Regency, Bangka Belitung Islands Province. The study used the experiment method with randomized block design (RBD) with three replications. The treatment used F7 upland rice lines and 5 genotypes as check varieties. The five of F7 lines used were 19I-06-09-23-03, 21B-57-21-21-23, 23F-04-10-18-18, 23A-56-20-07-20 and 23A-56-22-20-05. The five check viarieties used were Danau Gaung, Inpago 8, Inpago 12, Rindang and Situ Patenggang. Data was analyzed using ANOVA and Least Significant Increase (LSI) test. The results showed that 2 lines had a higher yield than the comparison genotypes were 23A-56-22-20- 05 and 23F-04-10-18-18. Hence, the recommended lines as the candidates for superior varieties with lodging resistance are 23A-56-22-20-05 for red rice type and 23F-04-10-18-18 for white rice type.

scholarly journals Evaluation Agronomy character of irradiated black rice Cempo Ireng mutant strains MS with 300 Gy of gamma rays

2020 ◽  
Author(s):  
Nandariyah ◽  
Martheffany Devitha P. P. ◽  
Parjanto ◽  
Suharyana ◽  
Riyatun ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Black Rice ◽  
Mutant Strains

scholarly journals ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA

2017 ◽  
Vol 10 (6) ◽  
pp. 377 ◽  
Author(s):  
Sukrasno Sukrasno ◽  
Slamet Tuty ◽  
Irda Fidrianny
Keyword(s):  
Rice Bran ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Red Rice ◽  
Black Rice ◽  
Dpph Assay ◽  
Rice Bran Extract ◽  
Dpph Scavenging

Objectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and carotenoid content, antioxidant activities using FRAP and DPPH assays were performed by UV-visible spectrophotometry and its correlation with EC50 of FRAP capacities and IC50 of DPPH scavenging activities were analyzed by Pearson’s method. Results: Ethanolic rice bran extract of black rice showed the lowest EC50 of FRAP capacity 64.35 µg/ml and IC50 of DPPH scavenging activity 23.92 µg/ml. The highest phenolic content, flavonoid content and carotenoid content were also given by ethanolic rice bran extract of black rice. There were significantly negative correlation between total phenolic content and carotenoid content in rice bran extract of red rice and black rice with their IC50 of DPPH. Conclusions: All of rice bran extracts (except n-hexane rice bran extract of black rice and ethanolic rice bran extract of white rice) were very strong antioxidant, by DPPH assay. Phenolic and carotenoid compounds in rice bran extracts of red rice and black rice were the major contributor in antioxidant activity by DPPH assay. Rice bran extracts of black rice had linear results by FRAP and DPPH assays. 

scholarly journals Extraction and Characterization of Native Starch From Black and Red Rice

2020 ◽  
Vol 8 (3) ◽  
pp. 1
Author(s):  
Henrique Valentim Moura ◽  
Rennan Pereira de Gusmão ◽  
Thaisa Abrantes Souza Gusmão ◽  
Deise Souza de Castro ◽  
Raphael Lucas Jacinto Almeida ◽  
...  
Keyword(s):  
Lower Cost ◽  
Extraction Yield ◽  
Rice Starch ◽  
Sodium Metabisulfite ◽  
Red Rice ◽  
Black Rice ◽  
Alternative Source ◽  
Rice Grains ◽  
Starch Gels

The objective of the study is to extract and characterize red rice starch, a grain commonly consumed in the Northeast region of Brazil and to compare it with the starch obtained from black rice, widely spread in eastern countries. Starch was extracted by immersion of rice in sodium metabisulfite (0.2%), followed by milling, filtration, decantation and drying at 50 °C. The obtained starches were characterized by the following parameters: physicochemical, physical, rheological, textural, structural, thermal and morphological. The highest starch extraction yield was found in red rice grains (67.84%), where it obtained higher water absorption and lower apparent viscosity, firmness and gum. Both starch gels indicate the behavior of non-Newtonian fluid in the specific case of pseudoplastic crystallinity type A and with polyhedral geometry. For the thermal analysis of red rice starch, it was observed higher gelatinization temperature and lower gelatinization enthalpy. Black and red rice grains can be considered an alternative source of starch, especially red rice, which has a lower cost due to its production in the country.

scholarly journals Evaluation of radioprotective effect of chickpea (Cicer arietinum) in the survival of Lasioderma serricorne (Fabricius, 1792) (Coleoptera, Anobìidae) irradiated with 60Co

2019 ◽  
Author(s):  
Adilson C. Barros ◽  
Kayo Okazaki ◽  
Valter Arthur
Keyword(s):  
Gamma Rays ◽  
First Generation ◽  
Biological Effects ◽  
Control Diet ◽  
Whole Grains ◽  
Lasioderma Serricorne ◽  
Protective Properties ◽  
Summary Statement ◽  
Set Up ◽  
Radioprotective Properties

ABSTRACTWe investigated the presence of natural radioprotectors in food using a new technical modality that utilizes the insect Lasioderma serricorne as a radiosensitivity bioindicator to check radioprotection properties in minimally processed chickpeas. The insects were obtained from the entomological biotherium of the Laboratory of Radiobiology and Environment of CENA-USP. They were fed with an experimental diet and just when the first generation hatched completely, the experiments were conducted. The randomly chosen control diet, consisted of three parts of wheat germ, one part of brewer’s yeast, and a slice of French bread toasted in an oven previously set up for humidity control. The diet of chickpeas consists only of whole grains crushed in a mechanical grinder to obtain flour. The result was significant for the survival of insects (p<0.0001) reared on a diet of chickpeas compared to those reared on control diet irradiated with gamma rays from 60Co in the range of 5.0 to 1500 Gy. We presented statistical evidence that the chickpea diet has radioprotective properties in the insect for gamma rays.SUMMARY STATEMENTThe study is important because it shows that chickpea has protective properties against ionizing radiation, how to act against its biological effects and minimize them.

scholarly journals Winter wheat (triticum aestivum l.) mutation depression under gamma-rays action

2021 ◽  
Author(s):  
M. Nazarenko ◽  
◽  
Y. Lykholat ◽  
V. Savosko ◽  
◽  
...  
Keyword(s):  
Winter Wheat ◽  
Plant Height ◽  
Gamma Rays ◽  
First Generation ◽  
Triticum Aestivum L ◽  
Pollen Sterility ◽  
Wheat Varieties ◽  
Research Fields ◽  
Yield Structure ◽  
Local Breeding

The purpose of our investigation was to identify the features of the depressive effects of the aftereffect of different doses of gamma-rays on winter wheat varieties of local breeding in terms of germination, survival, morphometry, yield. The parameters of germination and survival, the passage of the main phases of ontogenesis in winter wheat plants of French varieties (Courtiot and Gallixe) at the first generation were studied. The influence of mutagenic depression on parameters of yield structure (morphometry of mature plants) was established and the level of their variability was estimated too. In 2019 - 2020, experiments were conducted in the research fields of the research center of the Dnieper State Agrarian and Economic University. The experiments used seeds of Courtiot and Gallixe varieties, irradiated with gamma rays in doses of 100, 150, 200, 250, 300 Gy. Control was dry seeds. The variety Gallixe can be classified as resistant to gamma-rays, the variety Courtiotwas corresponded to high-sensitive. Parameter of germination and survival were directly correlated with increasing dose, with a dose of 200 Gy already semi-lethal, a dose of 250 Gy for variety Courtiot was sublethal, 300 Gy doze full-lethal, for the variety Gallixe sublethal was 300 Gy dose by which almost no plant material was obtained. Such parameters as plant height, weight of grain from the main spike and weight of thousand grains, partially (except for doses of 100-150 Gy) weight of grain from the plant reliably reproduce the mutagenic depression. Depression of the variety is affected by the dose of mutagen more than the genotype of the variety; the plant height parameter clearly demonstrates mutagenic depression. According to the results of factor and discriminant analysis as indicators affected by genotype-mutagenic interaction should be used germination and survival, pollen sterility, plant height, grain weight per spike, weight of thousand grains. Doses of 100 - 200 Gy are optimal for further use to obtain mutations; it is possible to use a dose of 250 Gy for the variety Gallixe.

scholarly journals Character Improvement of Red Rice (Oryza Sativa L.) Cv. Barak Cenana by Mutagenesis using Gamma Irradiation

2021 ◽  
Vol 21 (2) ◽  
pp. 305
Author(s):  
Aloysia Sri Pujiyanti ◽  
Bintang Kerta Wijaya ◽  
Ida Bagus Made Artadana ◽  
Popy Hartatie Hardjo ◽  
Maria Goretti Marianti Purwanto
Keyword(s):  
Gamma Rays ◽  
Dna Analysis ◽  
Oryza Sativa L ◽  
Random Amplified Polymorphic Dna ◽  
Tall Stature ◽  
Harvest Time ◽  
Red Rice ◽  
Wild Type ◽  
Genetic Changes ◽  
Phenotypic Characters

Red rice cv. Barak Cenana is a local Indonesian rice which is widely planted in Tabanan Regency, Bali. Barak Cenana red rice has potential as a functional food because it contains a lot of nutrients. However, this rice has an unfavorable character, such as long harvest time so that it can only be harvested once a year, and tall stature that causes plants to lodge easily, reducing its productivity. This study aims to determine the effect of gamma rays on growth and development as well as changes in character to obtain mutants plants that have better character. In this research, Barak Cenana seeds were irradiated using gamma rays with irradiation doses of 100 gy, 200 gy, 400 gy, and 800 gy. Furthermore, radiated seeds were planted in the greenhouse and characterized during the vegetative and generative phases including shoot length, harvest time, chlorophyll content, the number of productive tillers, the number of grain contents, the weight of 1,000 seeds, and the polymorphism profile using Random Amplified Polymorphic DNA Analysis (RAPD). The results of this study indicate that all radiation doses produce plants with random characters. Mutations using gamma rays at doses of 100 gy and 400 gy produced plants with better phenotypic characters than wild-type that is shorter plants, shorter harvest times, and more grain content. In addition, the results of the RAPD analysis confirm that there are genetic changes in irradiated rice. This mutants has the potential to reproduce germplasm and to obtain the next generation of mutants that have higher productivity and shorter plant heights.

scholarly journals The study of spectral uv-vis on coconut water as flour solvent of soybean, black rice and red rice

2021 ◽  
Vol 743 (1) ◽  
pp. 012079
Author(s):  
K Rahmawati ◽  
N D Kristanti ◽  
Sandriakana ◽  
S B Sumitro ◽  
S Widyarti
Keyword(s):  
Coconut Water ◽  
Red Rice ◽  
Black Rice

scholarly journals NMR metabolite comparison of local pigmented rice in Yogyakarta

2018 ◽  
Vol 22 (2) ◽  
pp. 68 ◽  
Author(s):  
Dio N. Wijaya ◽  
Febri Adi Susanto ◽  
Yekti Asih Purwestri ◽  
Dyah Ismoyowati ◽  
Tri Rini Nuringtyas
Keyword(s):  
Internal Standard ◽  
Anthocyanin Content ◽  
Rice Cultivars ◽  
Red Rice ◽  
Black Rice ◽  
Score Plot ◽  
Pigmented Rice ◽  
Positive Effects ◽  
Red Color ◽  
Metabolite Profiles

Pigmented rice may have a black or red color due to higher anthocyanin content in its grain. A natural antioxidant, many studies on anthocyanin have reported its positive effects on human health. This fact has spurred the development of pigmented rice as a functional food. This study aimed to compare the metabolite profiles of black and red rice. Three black rice cultivars, namely Melik, Pari Ireng, and Cempo Ireng Sleman, and two red rice cultivars, Inpari 24 and RC 204, were used. After husk removal, grain samples were ground in liquid nitrogen and dried with a freeze dryer. The dried samples were extracted using 50% MeOD4 (in a D2O phosphate buffer pH 6 containing 0.01% TSP as an internal standard). Metabolomic analysis was performed using 500 MHz NMR followed by multivariate data analysis. An orthogonal partial least squares-discriminant analysis (OPLS-DA) model ađer PCA was constructed to discriminate between the five different cultivars. The resulting OPLS-DA score plot revealed a clear separation between black rice and red rice. The metabolites that could influence the separation of red rice and black rice were valine, threonine, alanine, glutamate, galactinol, β-glucose, α-glucose, raffinose, and fumaric acid.

scholarly journals Black Rice Developed Through Interspecific Hybridization (O. sativa x O. rufipogon): Origin of Black Rice Gene from Indian Wild Rice

2020 ◽  
Author(s):  
Subhas Chandra Roy ◽  
Pankaj Shil
Keyword(s):  
Interspecific Hybridization ◽  
Wild Rice ◽  
Genetic Background ◽  
Promoter Region ◽  
Rice Grain ◽  
Red Rice ◽  
Black Rice ◽  
Rice Gene ◽  
Cultivated Rice ◽  
Recombination Mechanism

AbstractRice (Oryza sativa L.) is a most important staple food grain consumed by more than half of the world’s population. Wild rice (O. rufipogon Griff.) is considered as the immediate ancestral progenitor of cultivated rice O. sativa, evolved through the process of domestication. Most of the cultivated rice produced grains with white pericarp, but can also produce grains with brown, red and black (or purple rice) pericarp. Red rice pericarp accumulates proanthocyanidin whereas black rice contains anthocyanin, both have antioxidant activity and health benefits. Black pericarp is predicted to be regulated by alleles of three genetic loci- Kala1, Kala3, and Kala4. Recombinational and insertional genetic rearrangement in the promoter region of Kala4 is crucial for the development of black pericarp in rice grain. In the present study, we report first time in the breeding history that aromatic black rice lines were developed through interspecific hybridization and introgression in the genetic background of O. sativacv. Badshabhog, Chenga and Ranjit. Badshabhog and Ranjit is white grain rice but Chenga is red rice category. Common Asian wild rice O. rufipogon is used as donor parent (red grain) and source of black rice gene. Several possible genetic explanations have come up for the creation of black rice pericarp in the progeny lines. Possible reason may be the rearrangement and insertion of LINE1 in the promoter region of Kala4 allele through recombination mechanism leading to ectopic expression of Kala4 gene for the accumulation of anthocyanin and resulted in black rice formation. Other genes and regulatory factors may be induced and become functional to produce black pericarp. Black pericarp colour appeared in F2 populations in the wide crosses (Badshabhog x O. rufipogon and Chenga x O. rufipogon) but not in the cross with (Ranjit x O. rufipogon). Black pericarp trait inherited in F4 and F5 population with segregation phenotypes.This is a first report in the history of rice genetics and pre-breeding research, that black rice has been created through wide crossing and introgression by combining wild rice O. rufipogon in the genetic background of O. sativa. Present experimental evidence provides a new model of black rice origin. Thus, black rice (indica type) of Indian subcontinent originated independently through natural out crossing and artificial selection in the course of domestication.

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