scholarly journals Evaluation of two anaerobic systems for isolation of anaerobes

2011 ◽  
Vol 2 (1) ◽  
pp. 24 ◽  
Author(s):  
Louis Osayenum Egwari ◽  
Maria Olanike Buraimoh ◽  
Nkiru Nneye Nwokoye
Keyword(s):  
Transport System ◽  
Middle Ear ◽  
Anaerobic Bacteria ◽  
Time Lapse ◽  
Anaerobic Incubation ◽  
Isolation Rate ◽  
Clinical Specimens ◽  
Specimen Transport ◽  
Set Up

Many systems are available for the isolation of anaerobic bacteria from clinical specimens. The jar system is the oldest and more adapted while the pouches are not popular with many investigators. The anaerobic chambers are expensive to maintain and technically inflexible. This study evaluated the efficacy of the Oxoid anaerobic jar and the GENbag pouches as anaerobic incubation systems. Anaerobic cultures were set up for 145 middle ear exudates and incubation was in the anaerobic jar, GENbag or a combination of both. The effect of specimen transport system and time lapse before culturing on the performance of the anaerobic systems were evaluated Ten genera of anaerobic bacteria were isolated with both systems (P &gt; 0.05). <em>Peptostreptococcus</em> and <em>Prevotella</em> were isolated more frequently in Oxoid jar than in GENbag (P &lt; 0.05) but both systems were not discriminatory for <em>Clostridium</em>, <em>Propionibacterium</em> and <em>Veillonella</em>. The use of GENbag as a backup to Oxoid jar increased isolation rate from 56.6% to 90.3% (P &gt; 0.05). Type of transport media or vehicle did not affect the recovery of anaerobes adversely as did delay in processing of specimen. A careful application of a number of variables may improve isolation of anaerobes from clinical specimens

scholarly journals Evaluation of Port-A-Cul transport system for protection of anaerobic bacteria

1978 ◽  
Vol 8 (1) ◽  
pp. 28-35
Author(s):  
E Mena ◽  
F S Thompson ◽  
A Y Armfield ◽  
V R Dowell ◽  
D J Reinhardt
Keyword(s):  
Ambient Temperature ◽  
Transport System ◽  
Anaerobic Bacteria ◽  
Becton Dickinson ◽  
Bacterial Colony ◽  
Aerobic Conditions ◽  
Clinical Specimens ◽  
Obligately Anaerobic ◽  
Low Concentrations ◽  
Quantitative Recovery

The protection of anaerobes in Port-A-Cul (PAC) transport system (Bioquest, Div. of Becton, Dickinson &Co., Cockeysville, Md.) tubes and vials was studied. Ten species of obligately anaerobic bacteria commonly isolated from clinical specimens were used to prepare simulated swab and fluid specimens in high and low concentrations. Samples in PAC tubes and vials were held for 2, 24, and 48 h at ambient temperature and in a refrigerator. In addition, samples of the simulated specimens were exposed to controlled anaerobic and aerobic conditions in vented tubes and vials, with and without PAC medium, at ambient and refrigerator temperatures. Viable bacterial colony counts from specimens in PAC tubes and vials used as recommended by the manufacturer were consistently greater than those from specimens exposed to the different controlled conditions. The protection in PAC was about equal for specimens with either high or low concentrations of bacteria. Protection of the anaerobes in PAC was more obvious with swab than with fluid specimens. Quantitative recovery of anaerobes from refrigerated PAC samples, with few exceptions, was comparable to that from PAC samples held at ambient temperature.

scholarly journals Evaluation of a Novel Specimen Transport System (Venturi Transystem) for Anaerobic Bacteria

1997 ◽  
Vol 25 (s2) ◽  
pp. S132-S133 ◽  
Author(s):  
Marie K. Hudspeth ◽  
Diane M. Citron ◽  
Ellie J. C. Goldstein
Keyword(s):  
Transport System ◽  
Anaerobic Bacteria ◽  
Specimen Transport

scholarly journals Effects of Storage in an Anaerobic Transport System on Bacteria in Known Polymicrobial Mixtures and in Clinical Specimens

1978 ◽  
Vol 8 (6) ◽  
pp. 680-688
Author(s):  
Gale B. Hill
Keyword(s):  
Transport System ◽  
Staphylococcus Epidermidis ◽  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Aerobic Bacteria ◽  
Prolonged Storage ◽  
Clinical Specimens ◽  
Concentration Changes ◽  
Catalytic Removal

An anaerobic transport system (ATS) which provides for catalytic removal of oxygen was evaluated by using in vitro-prepared polymicrobial mixtures of logphase bacteria and clinical specimens. Inoculated swabs were stored at room temperature in (i) aerobic, (ii) anaerobic glove box, and (iii) ATS environments, and bacteria were quantitated after 2, 24, 48, and 72 h. Bacteria in a three-part mixture of Bacteroides fragilis, Peptostreptococcus anaerobius , and Escherichia coli and in a five-part mixture of B. fragilis, P. anaerobius, Fusobacterium nucleatum, Staphylococcus epidermidis , and Pseudomonas aeruginosa survived 72 h of storage in the ATS and anaerobic glove box environments, but the anaerobic species were inactivated in the aerobic storage except for B. fragilis in pure culture or in the three-part mixture. Changes in relative proportions among the species in a mixture were least in the ATS and anaerobic glove box environments and greatest during the aerobic storage, particularly in the five-part mixture. Bacteria present in pure or mixed culture in clinical specimens generally survived 72 h of storage in the ATS. These data indicate that changes in relative proportions occur with prolonged storage even under anaerobic conditions, but that the ATS would be most effective for preserving anaerobic bacteria and preventing drastic concentration changes and overgrowth of facultative and aerobic bacteria.

scholarly journals Improved Procedure for Transport of Dental Plaque Samples and Other Clinical Specimens Containing Anaerobic Bacteria

1979 ◽  
Vol 9 (5) ◽  
pp. 637-639
Author(s):  
Carol A. Spiegel ◽  
Glenn E. Minah ◽  
George N. Krywolap
Keyword(s):  
Transport System ◽  
Recovery Rate ◽  
Dental Plaque ◽  
Anaerobic Bacteria ◽  
Clinical Specimens

An improved transport system for samples containing anaerobic bacteria was developed. This system increased the recovery rate of anaerobic bacteria up to 28.8% as compared to a commonly used method.

Aerobic and Anaerobic Bacteria in the Middle ear and ear Canal in Acute Otitis Media

1982 ◽  
Vol 93 (sup386) ◽  
pp. 100-102 ◽  
Author(s):  
J. Luotonen ◽  
A. M. M. Jokipii ◽  
P. Sipilä ◽  
J. Väyrynen ◽  
L. Jokipii ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Anaerobic Bacteria ◽  
Acute Otitis Media ◽  
Ear Canal ◽  
Aerobic And Anaerobic

scholarly journals Integrated Passenger Transport System in Rural Areas – A Literature Review

2020 ◽  
Vol 32 (6) ◽  
pp. 863-873
Author(s):  
Branimir Maretić ◽  
Borna Abramović
Keyword(s):  
Literature Review ◽  
Transport System ◽  
Rural Areas ◽  
Urban Areas ◽  
Planning System ◽  
Passenger Transport ◽  
Public Transport System ◽  
Urban And Rural Areas ◽  
Set Up ◽  
The Impact

The planning and organisation of public passenger transport in rural areas is a complex process. The transport demand in rural areas is often low, which makes it hard to establish and run a financially sustainable public transport system. A solution is integrated passenger transport that eliminates deficiencies and provides benefits for all participants in the public passenger transport process. This paper describes the impact of integrated passenger transport on mobility in rural areas and critically evaluates different literature sources. Integration of passenger transport in urban areas has been described in the context of rural areas, and the challenges of integration of public passenger transport specific to rural areas have been analysed. Through the application in urban and rural areas, the planning of integrated and non-integrated passenger transport has been functionally analysed. The analysis found an increase in the degree of mobility in the areas that use integrated passenger transport compared to the non-integrated one. This research of the literature review has identified the rural areas of mobility as under-researched. The mobility research can set up a more efficient passenger transport planning system in rural areas.

scholarly journals A Case of Liver Abscess withDesulfovibrio desulfuricansBacteremia

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Saho Koyano ◽  
Keita Tatsuno ◽  
Mitsuhiro Okazaki ◽  
Kiyofumi Ohkusu ◽  
Takashi Sasaki ◽  
...  
Keyword(s):  
Liver Abscess ◽  
Clinical Presentation ◽  
Anaerobic Bacteria ◽  
Desulfovibrio Desulfuricans ◽  
Clinical Specimens ◽  
Sulfate Reducing ◽  
Oral Amoxicillin ◽  
First Case ◽  
Causative Agents ◽  
Amoxicillin Clavulanic Acid

Desulfovibriospp. are gram-negative, sulfate-reducing, and anaerobic bacteria found in the digestive tract of humans. BecauseDesulfovibriospp. are infrequent causative agents of infectious diseases and are difficult to isolate and to identify from clinical specimens, the appropriate antibiotic therapy to infection withDesulfovibriospp. has not been determined. We report the first case of liver abscess with bacteremia due toDesulfovibrio desulfuricansto show the clinical presentation and treatment. The patient was successfully treated with intravenous piperacillin-tazobactam and oral amoxicillin-clavulanic acid.

A Practical Technique for Tympanocentesis for Culturing Aerobic and Anaerobic Bacteria

PEDIATRICS ◽  
1980 ◽  
Vol 65 (3) ◽  
pp. 626-627
Author(s):  
Itzhak Brook
Keyword(s):  
Middle Ear ◽  
Negative Pressure ◽  
Anaerobic Bacteria ◽  
Acute Otitis Media ◽  
Clinical Settings ◽  
Middle Ear Fluid ◽  
Simplified Method ◽  
Medical Indications ◽  
Aerobic And Anaerobic

Tympanocentesis is a method frequently used to aspirate middle ear fluid from infected ears. There are some medical indications to perform tympanocentesis in certain clinical settings.1 This procedure could be beneficial in some patients in which determination of the etiology of the acute otitis media and relieving the pain and acute symptoms is of importance. The instrument frequently used by otolaryngologists is the Senturia ear specimen collector (Storz Instrument Company, St Louis) which operates by controlled suctioning using an external negative pressure. A simplified method for performing myringotomy and tumpanocentesis2 that avoids the use of complicated equipment and prevents contamination of the specimen is presented here.

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