scholarly journals Evaluation of a Novel Specimen Transport System (Venturi Transystem) for Anaerobic Bacteria

1997 ◽  
Vol 25 (s2) ◽  
pp. S132-S133 ◽  
Author(s):  
Marie K. Hudspeth ◽  
Diane M. Citron ◽  
Ellie J. C. Goldstein
Keyword(s):  
Transport System ◽  
Anaerobic Bacteria ◽  
Specimen Transport

scholarly journals Evaluation of two anaerobic systems for isolation of anaerobes

2011 ◽  
Vol 2 (1) ◽  
pp. 24 ◽  
Author(s):  
Louis Osayenum Egwari ◽  
Maria Olanike Buraimoh ◽  
Nkiru Nneye Nwokoye
Keyword(s):  
Transport System ◽  
Middle Ear ◽  
Anaerobic Bacteria ◽  
Time Lapse ◽  
Anaerobic Incubation ◽  
Isolation Rate ◽  
Clinical Specimens ◽  
Specimen Transport ◽  
Set Up

Many systems are available for the isolation of anaerobic bacteria from clinical specimens. The jar system is the oldest and more adapted while the pouches are not popular with many investigators. The anaerobic chambers are expensive to maintain and technically inflexible. This study evaluated the efficacy of the Oxoid anaerobic jar and the GENbag pouches as anaerobic incubation systems. Anaerobic cultures were set up for 145 middle ear exudates and incubation was in the anaerobic jar, GENbag or a combination of both. The effect of specimen transport system and time lapse before culturing on the performance of the anaerobic systems were evaluated Ten genera of anaerobic bacteria were isolated with both systems (P &gt; 0.05). <em>Peptostreptococcus</em> and <em>Prevotella</em> were isolated more frequently in Oxoid jar than in GENbag (P &lt; 0.05) but both systems were not discriminatory for <em>Clostridium</em>, <em>Propionibacterium</em> and <em>Veillonella</em>. The use of GENbag as a backup to Oxoid jar increased isolation rate from 56.6% to 90.3% (P &gt; 0.05). Type of transport media or vehicle did not affect the recovery of anaerobes adversely as did delay in processing of specimen. A careful application of a number of variables may improve isolation of anaerobes from clinical specimens

Anaerobic specimen transport system

1978 ◽  
Vol 34 (7) ◽  
pp. 851-851
Author(s):  
W. Scott Ramsey ◽  
W. Hertl
Keyword(s):  
Transport System ◽  
Specimen Transport

scholarly journals Evaluation of Port-A-Cul transport system for protection of anaerobic bacteria

1978 ◽  
Vol 8 (1) ◽  
pp. 28-35
Author(s):  
E Mena ◽  
F S Thompson ◽  
A Y Armfield ◽  
V R Dowell ◽  
D J Reinhardt
Keyword(s):  
Ambient Temperature ◽  
Transport System ◽  
Anaerobic Bacteria ◽  
Becton Dickinson ◽  
Bacterial Colony ◽  
Aerobic Conditions ◽  
Clinical Specimens ◽  
Obligately Anaerobic ◽  
Low Concentrations ◽  
Quantitative Recovery

The protection of anaerobes in Port-A-Cul (PAC) transport system (Bioquest, Div. of Becton, Dickinson &Co., Cockeysville, Md.) tubes and vials was studied. Ten species of obligately anaerobic bacteria commonly isolated from clinical specimens were used to prepare simulated swab and fluid specimens in high and low concentrations. Samples in PAC tubes and vials were held for 2, 24, and 48 h at ambient temperature and in a refrigerator. In addition, samples of the simulated specimens were exposed to controlled anaerobic and aerobic conditions in vented tubes and vials, with and without PAC medium, at ambient and refrigerator temperatures. Viable bacterial colony counts from specimens in PAC tubes and vials used as recommended by the manufacturer were consistently greater than those from specimens exposed to the different controlled conditions. The protection in PAC was about equal for specimens with either high or low concentrations of bacteria. Protection of the anaerobes in PAC was more obvious with swab than with fluid specimens. Quantitative recovery of anaerobes from refrigerated PAC samples, with few exceptions, was comparable to that from PAC samples held at ambient temperature.

scholarly journals Effects of Storage in an Anaerobic Transport System on Bacteria in Known Polymicrobial Mixtures and in Clinical Specimens

1978 ◽  
Vol 8 (6) ◽  
pp. 680-688
Author(s):  
Gale B. Hill
Keyword(s):  
Transport System ◽  
Staphylococcus Epidermidis ◽  
Anaerobic Bacteria ◽  
Fusobacterium Nucleatum ◽  
Aerobic Bacteria ◽  
Prolonged Storage ◽  
Clinical Specimens ◽  
Concentration Changes ◽  
Catalytic Removal

An anaerobic transport system (ATS) which provides for catalytic removal of oxygen was evaluated by using in vitro-prepared polymicrobial mixtures of logphase bacteria and clinical specimens. Inoculated swabs were stored at room temperature in (i) aerobic, (ii) anaerobic glove box, and (iii) ATS environments, and bacteria were quantitated after 2, 24, 48, and 72 h. Bacteria in a three-part mixture of Bacteroides fragilis, Peptostreptococcus anaerobius , and Escherichia coli and in a five-part mixture of B. fragilis, P. anaerobius, Fusobacterium nucleatum, Staphylococcus epidermidis , and Pseudomonas aeruginosa survived 72 h of storage in the ATS and anaerobic glove box environments, but the anaerobic species were inactivated in the aerobic storage except for B. fragilis in pure culture or in the three-part mixture. Changes in relative proportions among the species in a mixture were least in the ATS and anaerobic glove box environments and greatest during the aerobic storage, particularly in the five-part mixture. Bacteria present in pure or mixed culture in clinical specimens generally survived 72 h of storage in the ATS. These data indicate that changes in relative proportions occur with prolonged storage even under anaerobic conditions, but that the ATS would be most effective for preserving anaerobic bacteria and preventing drastic concentration changes and overgrowth of facultative and aerobic bacteria.

scholarly journals Improved Procedure for Transport of Dental Plaque Samples and Other Clinical Specimens Containing Anaerobic Bacteria

1979 ◽  
Vol 9 (5) ◽  
pp. 637-639
Author(s):  
Carol A. Spiegel ◽  
Glenn E. Minah ◽  
George N. Krywolap
Keyword(s):  
Transport System ◽  
Recovery Rate ◽  
Dental Plaque ◽  
Anaerobic Bacteria ◽  
Clinical Specimens

An improved transport system for samples containing anaerobic bacteria was developed. This system increased the recovery rate of anaerobic bacteria up to 28.8% as compared to a commonly used method.

scholarly journals Designing and Piloting a Specimen Transport System in Burkina Faso

2020 ◽  
Vol 18 (S1) ◽  
pp. S-98-S-104 ◽  
Author(s):  
Emilie Dama ◽  
Abdoulaye Nikiema ◽  
Kameko Nichols ◽  
Brice Wilfried Bicaba ◽  
Souleymane Porgho ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Transport System ◽  
Specimen Transport

Acute Suppurative Parotitis Caused by Anaerobic Bacteria: Report of Two Cases

PEDIATRICS ◽  
1978 ◽  
Vol 62 (6) ◽  
pp. 1019-1020
Author(s):  
I. Brook ◽  
S. M. Finegold
Keyword(s):  
Staphylococcus Aureus ◽  
Respiratory Tract ◽  
Anaerobic Bacteria ◽  
The Other ◽  
Upper Respiratory Tract ◽  
Anaerobic Culture ◽  
Culture Techniques ◽  
Specimen Transport ◽  
Predominant Pathogen ◽  
Upper Respiratory

Staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. Although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. It may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. We describe two cases of acute suppurative parotitis in which cultures yielded anaerobic bacteria. In one case, no aerobes were isolated; in the other, the anaerobe was the predominant organism numerically.

scholarly journals Extensive evaluation of fastidious anaerobic bacteria recovery from the Copan eSwab® transport system

2018 ◽  
Vol 144 ◽  
pp. 73-78 ◽  
Author(s):  
Thomas Demuyser ◽  
Deborah De Geyter ◽  
Daisy Van Dorpe ◽  
Kristof Vandoorslaer ◽  
Ingrid Wybo
Keyword(s):  
Transport System ◽  
Anaerobic Bacteria ◽  
Extensive Evaluation

On the structural organization of biological pumps and channels

1984 ◽  
Vol 42 ◽  
pp. 138-141
Author(s):  
G. Zampighi ◽  
M. Kreman
Keyword(s):  
Active Transport ◽  
Transport System ◽  
Plasma Membranes ◽  
Transport Proteins ◽  
Molecular Organization ◽  
Passive Transport ◽  
Concentration Gradients ◽  
Cell Functions ◽  
Natural Concentration ◽  
Active Transport System

The plasma membranes of most animal cells contain transport proteins which function to provide passageways for the transported species across essentially impermeable lipid bilayers. The channel is a passive transport system which allows the movement of ions and low molecular weight molecules along their concentration gradients. The pump is an active transport system and can translocate cations against their natural concentration gradients. The actions and interplay of these two kinds of transport proteins control crucial cell functions such as active transport, excitability and cell communication. In this paper, we will describe and compare several features of the molecular organization of pumps and channels. As an example of an active transport system, we will discuss the structure of the sodium and potassium ion-activated triphosphatase [(Na+ +K+)-ATPase] and as an example of a passive transport system, the communicating channel of gap junctions and lens junctions.

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