scholarly journals Detection of Gardnerella vaginalis, Candida spp. and Trichomonas vaginalis DNA in symptomatic women

2010 ◽  
Vol 25 (3) ◽  
Author(s):  
Vittorio Focarelli ◽  
Vincenzo Rondinelli ◽  
Maria Gabriella Lepore ◽  
Pasquale Minchella ◽  
Raffaele Saraceno ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Spp

scholarly journals Sexually transmitted diseases among women with habitual abortion

1998 ◽  
Vol 4 (2) ◽  
pp. 343-349
Author(s):  
N. K. Mahdi ◽  
M. M. Al Hamdani
Keyword(s):  
Sexually Transmitted Disease ◽  
Trichomonas Vaginalis ◽  
Transmitted Disease ◽  
Normal Pregnancy ◽  
Gardnerella Vaginalis ◽  
Significant Relation ◽  
Candida Spp ◽  
Sexually Transmitted ◽  
Habitual Abortion ◽  
Control Study

Sexually transmitted disease as a risk factor for habitual abortion was investigated in a case-control study in Basra between October 1994 and May 1995. Of 81 women with habitual abortion, 41 [50.6%] had a sexually transmitted disease. In the normal pregnancy group, 64 of 119 women [53.8%] had a sexually transmitted disease [X [2] = 0.712, P > 0.05]. The isolation rates of Candida spp., Trichomonas vaginalis, Gardnerella vaginalis and Neisseria gonorrhoeae in women with habitual abortion were not significantly different from those in normal pregnant women. No association was found between the presence of a sexually transmitted disease and habitual abortion [odds ratio = 0.9]. No positive case of syphilis was diagnosed using VDRL and TPHA tests. There was a significant relation between the presence of a sexually transmitted disease and the presence of vaginal discharge, vaginal pH > 4.5 and the frequency of sexual activity/week > 2

Evaluation of Antimicrobial Activity of Trachyspermum ammi (L.) Sprague Essential Oil and Its Active Constituent, Thymol, against Vaginal Pathogens

2020 ◽  
Author(s):  
Mozhgan MehriArdestani ◽  
Atousa Aliahmadi ◽  
Tayebeh Toliat ◽  
Abdolhossein Dalimi ◽  
Zohreh Momeni ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Trichomonas Vaginalis ◽  
Antimicrobial Agents ◽  
Gardnerella Vaginalis ◽  
Active Constituent ◽  
Candida Spp ◽  
Vaginal Infections ◽  
Trachyspermum Ammi ◽  
Conventional Antibiotics

Vaginal infections are one of the major reasons women visit a gynecologist. Increased resistance to conventional antibiotics is one of the main factors mitigating the development of new antimicrobial agents, especially those of natural origin. In traditional Persian medicine, Trachyspermum ammi has been claimed to clear vagina from excessive discharge. Therefore, in this study, the antimicrobial activity of Ajwain essential oil was evaluated against some vaginal pathogens. The essential oil of ajwain was picked up and the minimum inhibitory and bactericidal concentrations (MIC and MBC) were revealed. The most frequently detected microorganisms involved in genital infections including Candida spp., Gardnerella vaginalis, Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Lactobacillus acidophilus were considered. Evaluation of the essential oil of Trichomonas vaginalis was done by calculation of percent of growth inhibition. The essential oil showed a remarkable activity against the studied bacteria and fungi with MIC at a range of 0.0315 - 0.5 mg/ml and MBC at a range of 0.125 - 4 mg /ml. The highest inhibition and bactericidal activity was observed in S. agalactiae and G. vaginalis. 100% inhibition of T. vaginalis growth was shown at a concentration of 2000 μg/ml after 48 h by essential oil. The antimicrobial activity of the essential oil was more than that of thymol. Supposedly essential oil of Trachyspermum ammi fruit could inhibit vaginal pathogens growth .Further preclinical and clinical studies are required to confirm the efficacy of this natural agent in vaginitis.

Validation of an immunologic diagnostic kit for infectious vaginitis by Trichomonas vaginalis, Candida spp., and Gardnerella vaginalis

2009 ◽  
Vol 63 (3) ◽  
pp. 257-260 ◽  
Author(s):  
Arsenio Betancourt Bravo ◽  
Lilian Sánchez Miranda ◽  
Octavio Fernández Lima ◽  
Alexandra Villoch Cambas ◽  
Maite Lorenzo Hernández ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Spp

scholarly journals Agents causing genital infections in routine cytological tests: frequency and characteristics of Papanicolaou smears

2022 ◽  
Vol 82 ◽  
Author(s):  
F. S. Carvalho ◽  
N. K. A. Porto ◽  
P. V. M. Azevedo ◽  
P. K. A. Magalhães ◽  
E. N. de Araújo ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Pap Smear ◽  
Clinical Pathology ◽  
Infectious Agent ◽  
Gardnerella Vaginalis ◽  
Pap Smears ◽  
Candida Spp ◽  
Pathology Laboratory ◽  
Genital Infections ◽  
Tract Infections

Abstract Urinary tract infections are responsible for most human infections, these are caused by bacteria, fungi, protozoa and associated microorganisms. The goal of this study was to determine the rate of vaginal infection-causing agents in routine cytological exams and also to evaluate the characteristics of positive tested Pap smears. A retrospective documental with descriptive aspect research was performed in a Clinical Pathology laboratory from Maceió-AL. The results of the Pap smears exams for Trichomonas vaginalis, Gardnerella vaginalis, Candida spp and HPV were arranged in a database as well as other data such as bacterial, protozoan, fungal and viral coinfections. The sample was composed by 18.645 women who have undergone Pap smear exams from 2013 to 2017. Of these analyzed exams, 27.4% in 2013, 10.9% in 2014, 10.6% in 2015, 15.2% in 2016 and 13.67% in 2017 were within normal range, however more than half of these exams presented some infections caused by unspecific or microbiological agents. By analyzing all the reports, 4.073 (21.84%) presented inflammations caused by some species of infectious agent with the following rate order: G. vaginalis and T. vaginalis. Furthermore, it was possible to confirm high rates of coinfection by and Candida spp. The rate of genital infections in this study highlights that there is a public health matter that must be controlled, which points a greater need for monitoring, guidance and actions towards greater awareness in order to prevent these problems.

scholarly journals Associação entre Vaginose Bacteriana e Anormalidades Citológicas nos Exames Citopatológicos Analisados em um Laboratório Escola de Goiânia-GO

2021 ◽  
Vol 67 (1) ◽  
Author(s):  
Isabelly Ribeiro Barbosa ◽  
Denes Santos Rodrigues ◽  
Luiz Henrique Alves Ferreira ◽  
Leonardo Luiz Borges ◽  
Andrea Alves Ribeiro
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Low Grade ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Candida Spp ◽  
Squamous Cells ◽  
Intraepithelial Lesion ◽  
Version 2.0 ◽  
Undetermined Significance

Introdução: A Gardnerella vaginalis facilita a infecção pelo papilomavírus humano (HPV). Objetivo: Verificar a associação entre anormalidades citológicas e presença de Gardnerella vaginalis nos esfregaços cervicovaginais encaminhados ao Laboratório Clínico da Pontifícia Universidade Católica de Goiás (LAC/PUC Goiás) estratificadas por faixa etária. Método: Estudo transversal realizado no LAC/ PUC Goiás entre janeiro de 2013 a dezembro de 2015. Para análises estatísticas, a variável idade foi categorizada em ≤39 anos e >40 anos, utilizando o programa IBM SPSS Statistics (Version 2.0, 2011®) para o teste de qui-quadrado (X²), com intervalo de confiança de 95% e valor p<0,05. Resultados: Foram analisados 4.558 exames citopatológicos, a maioria com presença de Lactobacillus spp. (46,97%). A prevalência dos agentes patogênicos foi a Gardnerella vaginalis (79,6%), seguida de Candida spp. (16,8%), Trichomonas vaginalis (2,2%), Herpes simplex (0,4 %) e Chlamydia trachomatis (0,1%). As anormalidades citológicas foram observadas em 9,1%, sendo atypical squamous cells of undetermined significance (ASC-US) 2,57%, low grade squamous intraepithelial lesion (LSIL) 1,78%, atypical squamous cells of undetermined significance cannot exclude high grade squamous intraepithelial lesion (ASC-H) 3,52%, high grade squamous intraepithelial lesion (HSIL) 1,08%, atypical endocervical cells, favor neoplastic (AGC-NEO) 0,22% e carcinoma 0,02%. Houve uma associação significante entre anormalidades citológicas graves e mulheres ≥40 anos, OR 3,01 (IC 95% 2,0-4,58) (p<0,0001). Mulheres ≤40 anos mostraram significância à presença de Gardnerella vaginalis (p<0,0004). Conclusão: Uma elevada prevalência de Gardnerella vaginalis foi encontrada associada com as anormalidades citológicas, principalmente em mulheres sexualmente ativas.

scholarly journals PREVALÊNCIA DE Gardnerella vaginalis, Candida spp. e Trichomonas vaginalis EM EXAMES CITOPATOLÓGICOS DE GESTANTES ATENDIDAS NO HOSPITAL UNIVERSITÁRIO DE SANTA MARIA-RS

2011 ◽  
Vol 36 (1) ◽  
pp. 37
Author(s):  
Gabriela Bonfanti ◽  
Thissiane De Lima Gonçalves
Keyword(s):  
Prenatal Care ◽  
Pregnant Women ◽  
Trichomonas Vaginalis ◽  
Pap Smear ◽  
Gardnerella Vaginalis ◽  
Candida Spp ◽  
Pap Smear Test ◽  
Microbiological Agents ◽  
Santa Maria ◽  
Lactobacillus Spp

Introdução: Durante a gestação podem ocorrer distúrbios no mecanismo fisiológico do trato genital, composto por Lactobacillus spp., resultando em processos infecciosos determinados por agentes bacterianos. Tais infecções podem levar a doença pélvica inflamatória, parto prematuro e infecção fetal, sendo que o exame de Papanicolaou pode sugerir a presença desses agentes infecciosos. Objetivo: Verificar a prevalência dos agentes microbiológicos encontrados no Papanicolaou de gestantes atendidas no HUSM. Métodos: Foi realizado um levantamento do arquivo dos laudos citológicos do Laboratório do Hospital Universitário de Santa Maria (HUSM) no período de janeiro de 2005 a dezembro de 2008, das gestantes que realizaram o exame citopatológico durante o pré-natal. Resultados: Foram analisados 1344 laudos e observou-se que 59,82% das pacientes apresentaram flora bacteriana normal, ou seja, composta porLactobacillus spp. O total de floras alteradas foi de 40,17%. Dessas, 38,24% apresentaram Gardnerella vaginalis, 33,75% Candida spp., 5,92% Trichomonas vaginalis, 21,54% flora mista (cocos e outros bacilos) e 0,54% outros microorganismos como Leptothrix vaginalis e Fuseobacterium spp. Conclusão: Essas infecções estão associadas à complicações na gestação e nossos resultados mostraram uma alta prevalência de agentes patogênicos nas pacientes, confirmando a importância do acompanhamento pré-natal das gestantes para o diagnóstico e tratamento adequado dessas possíveis infecções.Descritores: gestantes, exame citopatológico, agentes microbiológicos.  Introduction: During pregnancy may occur disorders in the physiological mechanism of genital tract, consisting ofLactobacillus spp., resulting in infectious processes determined by bacterial agents. These infections can lead to pelvic inflammatory disease, preterm delivery and fetal infection. The Pap Smear test may suggest the presence of these infectious agents. Objective: To determinate the prevalence of microbiological agents found in the Pap smear test of pregnant women attending in HUSM. Methods: It was made survey of the file of the cytological laboratory of the Hospital Universitario de Santa Maria (HUSM) from January 2005 to December 2008, belonging to women, who underwent the examination of cytopathologic during prenatal care. Results: 1344 reports were analyzed and indicated that 59.82% of patients showed normal bacterial flora, which is composed of Lactobacillus spp. The total flora switched was 40.17%. Of these, 38.24% had Gardnerella vaginalis, 33.75% Candida spp., 5.92% Trichomonas vaginalis, 21.54% mixed flora (bacilli and cocci) and 0.54% other microorganisms such as Leptothrix vaginalis and Fuseobacteriumspp. Conclusion: The microbial infections are associated with complications during pregnancy, our results showed a high prevalence of pathogens in patients, confirming the importance of prenatal care of pregnant women for diagnosis and proper treatment of possible infections.Descriptors: pregnant women, cervical screening, microbiological agents.  

scholarly journals Diagnóstico e orientações preventivas nas infecções cervico-vaginais e no câncer cervical

2016 ◽  
Vol 25 (1) ◽  
pp. 33
Author(s):  
Luciane Noal CALIL ◽  
Andréia BUFFON ◽  
Adelina MEZZARI
Keyword(s):  
Candida Albicans ◽  
Candida Glabrata ◽  
Trichomonas Vaginalis ◽  
Candida Parapsilosis ◽  
Gardnerella Vaginalis ◽  
Candida Spp

ObjetivoAvaliar o diagnóstico de câncer cervical e de infecções cervico vaginais, bem como posteriores orientações preventivas para estas doenças.MétodosForam avaliados esfregaços vaginais de exames citológicos e cultura para a pesquisa de Candida spp. nos materiais colhidos de pacientes atendidas em Unidades Básicas de Saúde do Sistema Único de Saúde.ResultadosFoi atendido um total de 78 mulheres com idades entre 15 e 81 anos, tendo como média de idade 39,8 anos e desvio-padrão de 15,6 anos. Na leitura da lâmina corada pelo método de Papanicolaou para adequação da amostra, 43 (55,1%) apresentaram presença de junção escamo-colunare 21 (26,9%) presença de metaplasia. Com relação à flora microbiana presente nas amostras, houve 27 amostras (34,6%) com flora lactobacilar, 40 (51,3%) com flora cocóide, 4 (5,1%) com presença de Candida spp. e Gardnerella vaginalis, 2 (2,6%) com Trichomonas vaginalis e em 26 casos (33,3%) outros bacilos foram observados. No laudo citológico, 32 amostras (41,0%) tiveram resultado normal, 34 (43,6%) com processo inflamatório, 6 (7,6%) apresentaram atrofia com inflamação, com 5 (6,4%) com processo inflamatório e reacional e uma (1,3%) com presença de células escamosas atípicas de significado indeterminado. Quanto à cultura para Candida spp., 55 (73,3%) amostras foram negativas e 20 (26,7%), positivas. Destas, 11 (14,1%) foram casos de Candida albicans isolada, 5 (6,7%) de Candida glabrata, 2 (2,7%) de Candida parapsilosis e outros 2 de Candida guilliermondii.ConclusãoO presente estudo demonstrou que mulheres com ou sem lesões precursoras do câncer do colo do útero, apresentavam também outras infecções que, de alguma forma, comprometem sua qualidade de vida, reforçando a aplicação de medidas preventivas para melhorar essas realidade.

FREQUÊNCIA DE TRICHOMONAS VAGINALIS, GARDNERELLA VAGINALIS E CANDIDA SPP. EM EXAMES COLPOCITOLÓGICOS EM VISTA SERRANA-PB

2018 ◽  
Vol 16 (2) ◽  
pp. 28-37
Author(s):  
Ravena A. Martins ◽  
Rafael S. Fernandes ◽  
Matheus A. Martins ◽  
Clélia A. X. Mota ◽  
Sócrates G. Santos ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Spp

Vaginitis: Review on Drug Resistance

2020 ◽  
Vol 21 (16) ◽  
pp. 1672-1686
Author(s):  
Graziela Vargas Rigo ◽  
Tiana Tasca
Keyword(s):  
Drug Resistance ◽  
Trichomonas Vaginalis ◽  
Hiv Transmission ◽  
Female Genital Tract ◽  
Age Groups ◽  
Well Being ◽  
Gardnerella Vaginalis ◽  
Candida Spp ◽  
Genital Tract Infections ◽  
Tract Infections

Female genital tract infections have a high incidence among different age groups and represent an important impact on public health. Among them, vaginitis refers to inflammation of the vulva and/or vagina due to the presence of pathogens that cause trichomoniasis, bacterial vaginosis, and vulvovaginal candidiasis. Several discomforts are associated with these infections, as well as pregnancy complications and the facilitation of HIV transmission and acquisition. The increasing resistance of microorganisms to drugs used in therapy is remarkable, since women report the recurrence of these infections and associated comorbidities. Different resistant mechanisms already described for the drugs used in the therapy against Trichomonas vaginalis, Candida spp., and Gardnerella vaginalis, as well as aspects related to pathogenesis and treatment, are discussed in this review. This study aims to contribute to drug design, avoiding therapy ineffectiveness due to drug resistance. Effective alternative therapies to treat vaginitis will reduce the recurrence of infections and, consequently, the high costs generated in the health system, improving women’s well-being.

Identificación de microorganismos asociados a erosión de cérvix en la Facultad de Medicina de la Benemérita Universidad Autónoma de Puebla, México

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
José Antonio Sánchez Hernández ◽  
Iván Meléndez García ◽  
Guillermo Muñoz Zurita
Keyword(s):  
Trichomonas Vaginalis ◽  
Gardnerella Vaginalis ◽  
Candida Spp ◽  
En 31 ◽  
Lactobacillus Spp ◽  
De Medicina

<p><strong>Objetivo: </strong>identificar el tipo de microorganismos que se encuentran asociados a erosión de cérvix. <strong>Métodos:</strong> se revisaron las citologías exfoliativas cérvico-vaginales de pacientes que acudieron al Laboratorio de Biología Celular de la Facultad de Medicina de la Benemérita Universidad Autónoma de Puebla (fmbuap), de 2007 a 2016. <strong>Resultados:</strong> de 1 260 citologías exfoliativas que se observaron, 254 (20.15%) cumplieron los criterios de inclusión al presentar erosión cervical. Al observar al microscopio los 254 exudados se registraron en 223 pacientes (87.79%) los siguientes hallazgos: 186 muestras (73.22%) revelaron la presencia de flora cocoide; 27 pacientes (10.62%) <em>Lactobacillus spp</em>, cinco (1.90%) presentaron <em>Candida spp</em>, tres laminillas (1.18%) con <em>Gardnerella vaginalis</em> (células clue) y en dos citologías (0.78%) se reportó <em>Trichomonas vaginalis</em>. En 31 muestras (12.20%) no se reveló la presencia de microorganismos. <strong>Conclusión:</strong> se identificaron los siguientes microorganismos asociados a erosión del cérvix: flora cocoide en exceso, <em>Candida spp</em>, <em>G. vaginalis</em> y <em>T. vaginalis</em>.</p>

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