scholarly journals Vaginal swabs are appropriate specimens for the diagnosis of genital tract infection with Chlamydia trachomatis

2008 ◽  
Vol 23 (2) ◽  
Author(s):  
Giacomo Audisio ◽  
Aldo Bossano ◽  
Denis Cauchi Inglott ◽  
Giovanni Orso Giacone ◽  
Maria Agnese Latino ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Tract Infection ◽  
Genital Tract ◽  
Genital Tract Infection ◽  
Vaginal Swabs

scholarly journals Vaginal Swabs Are Appropriate Specimens for Diagnosis of Genital Tract Infection with Chlamydia trachomatis

2003 ◽  
Vol 41 (8) ◽  
pp. 3784-3789 ◽  
Author(s):  
J. Schachter ◽  
W. M. McCormack ◽  
M. A. Chernesky ◽  
D. H. Martin ◽  
B. Van Der Pol ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Tract Infection ◽  
Genital Tract ◽  
Genital Tract Infection ◽  
Vaginal Swabs

A novel guinea pig model of Chlamydia trachomatis genital tract infection

Vaccine ◽  
2011 ◽  
Vol 29 (35) ◽  
pp. 5994-6001 ◽  
Author(s):  
Marien I. de Jonge ◽  
Sander A.S. Keizer ◽  
Hicham M. el Moussaoui ◽  
Lieke van Dorsten ◽  
Rima Azzawi ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Tract Infection ◽  
Guinea Pig ◽  
Genital Tract ◽  
Pig Model ◽  
Genital Tract Infection ◽  
Guinea Pig Model

scholarly journals Experimental Gonococcal Genital Tract Infection and Opacity Protein Expression in Estradiol-Treated Mice

1999 ◽  
Vol 67 (11) ◽  
pp. 5699-5708 ◽  
Author(s):  
Ann E. Jerse
Keyword(s):  
Tract Infection ◽  
Protein Expression ◽  
Genital Tract ◽  
Genital Tract Infection ◽  
Outbred Mouse ◽  
Vaginal Swabs ◽  
Selection For ◽  
Strain Fa1090 ◽  
Gonococcal Strain

ABSTRACT The development of effective prophylactic agents against gonorrhea and the study of adaptation by Neisseria gonorrhoeae to the urogenital mucosa are hindered by the lack of a well-established animal model of gonococcal genital tract infection. Here, a murine model of long-term gonococcal genital tract infection is described. Female BALB/c mice were treated with 17-β-estradiol and inoculated intravaginally with wild-type gonococcal strain FA1090 or MS11.N. gonorrhoeae was recovered from vaginal swabs for an average of 12 to 13 days following inoculation with 106 CFU of either strain. Inflammation occurred in over 80% of infected mice, and diplococci were associated with epithelial cells and neutrophils in stained vaginal smears. Ascended infection occurred in 17 to 20% of mice inoculated with strain FA1090. An outbred mouse strain (SLC:ddY) previously reported to be naturally susceptible to N. gonorrhoeae was also tested; however, as with BALB/c mice, estradiol was required for prolonged infection. Although piliation was not maintained during experimental murine infection, 46 to 100% of vaginal isolates from four of eight BALB/c mice and three of four SLC:ddY mice expressed one or more opacity (Opa) proteins within 4 days after inoculation with an Opa-negative variant of strain FA1090. The observed selection for and/or induction of gonococcal Opa protein expression during murine infection appears to parallel events that occur during experimental urethritis in volunteers.

scholarly journals Prior Genital Tract Infection with a Murine or Human Biovar of Chlamydia trachomatis Protects Mice against Heterotypic Challenge Infection

1999 ◽  
Vol 67 (6) ◽  
pp. 3019-3025 ◽  
Author(s):  
Kyle H. Ramsey ◽  
Todd W. Cotter ◽  
Rena D. Salyer ◽  
Gurwattan S. Miranpuri ◽  
Michael A. Yanez ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Tract Infection ◽  
Genital Tract ◽  
Primary Infection ◽  
Secondary Infection ◽  
Delayed Type Hypersensitivity ◽  
Genital Infection ◽  
Blast Transformation ◽  
Genital Tract Infection ◽  
Igg Antibody

ABSTRACT We sought to assess the degree of cross-protective immunity in a mouse model of chlamydial genital tract infection. Following resolution of genital infection with the mouse pneumonitis (MoPn) biovar ofChlamydia trachomatis, mice were challenged intravaginally with either MoPn or human serovar E or L2. The majority of animals previously infected with MoPn were solidly immune to challenge with either of the two human biovars. Surprisingly, approximately 50% of animals became reinfected when homologously challenged with MoPn, although the secondary infection yielded significantly lower numbers of the organism isolated over a shorter duration than in the primary infection. Primary infection with serovar E also protected against challenge with MoPn or serovar L2, although the degree of immune protection was lower than that resulting from primary infection with MoPn. Blast transformation and assessment of delayed-type hypersensitivity indicated that mice previously infected with either human or murine biovars produced broadly cross-reactive T cells that recognized epitopes of either murine or human biovars of C. trachomatis. Immunoblotting demonstrated that primary MoPn infection produced immunoglobulin G (IgG) antibody to antigens of MoPn as well as at least three distinct antigenic components of human serovar E, one of which was identical in molecular weight to the major outer membrane protein (MOMP). Primary infection with serovar E produced IgG antibody reactive against serovar E but not MoPn MOMP and against at least one ca. 60-kDa protein of both chlamydial strains. Our results indicate that primary genital infection of mice with murineC. trachomatis induces immunity against challenge with either of two human biovars.

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