scholarly journals Preliminary data on the antimicrobial effect of Cannabis sativa L. variety Futura 75 against food-borne pathogens in vitro as well as against naturally occurring microbial populations on minced meat during storage

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Frédérique Pasquali ◽  
Marco Schinzari ◽  
Alex Lucchi ◽  
Mara Mandrioli ◽  
Tullia Gallina Toschi ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Naturally Occurring ◽  
Food Borne ◽  
Minced Beef ◽  
Microbial Groups ◽  
Staphylococcus Spp ◽  
Minced Meat

In the present study, the antimicrobial effect of Cannabis sativa Futura 75 was evaluated both in vitro against foodborne bacterial pathogens, and on food against naturally occurring microbial groups of minced meat stored for 8 days at 4°C. Ethanol extraction was performed on the grind of the inflorescence. After extraction, ethanol was completely evaporated and substituted by water. Serial dilutions of the extract, the grind and cannabidiol 99% were added to Nutrient Agar and spotted with Listeria monocytogenes, Salmonella Typhimurium, Escherichia coli and Staphylococcus spp. Regarding the evaluation on food, 50 mL of extract, characterised by CBD at concentration of 322,70 μg/mL, were added to 2.5 kg of minced beef meat. Meat was divided into aliquots and stored for 8 days at 4°C. At 0, 1, 2, 3, 4, 7, and 8 days, aerobic bacteria, enterobacteria, coliforms and E. coli were enumerated. All tested products were efficient against Gram +. In particular, extract corresponding to CBD concentration of 0.017 and 0.3 mg/mL were effective against L. monocytogenes and Staphylococcus spp. respectively. After 8 days of storage at 4°C, treated minced meat showed a bright red colour in comparison to a brownish control meat. Moreover, Enterobacteriaceae and coliforms were significantly reduced of 2.3 log CFU/g and 1.6 log CFU/g respectively in treated meat in comparison to the control. Although preliminary, the present study suggests the antimicrobial properties of the extract of Cannabis sativa both in vitro and in minced meat.

scholarly journals Nanovesicles Loaded with Origanum onites and Satureja thymbra Essential Oils and Their Activity against Food-Borne Pathogens and Spoilage Microorganisms

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2124
Author(s):  
Giulia Vanti ◽  
Ekaterina-Michaela Tomou ◽  
Dejan Stojković ◽  
Ana Ćirić ◽  
Anna Rita Bilia ◽  
...  
Keyword(s):  
Essential Oils ◽  
Propylene Glycol ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Spoilage Microorganisms ◽  
Satureja Thymbra ◽  
Origanum Onites

Food poisoning is a common cause of illness and death in developing countries. Essential oils (EOs) could be effective and safe natural preservatives to prevent and control bacterial contamination of foods. However, their high sensitivity and strong flavor limit their application and biological effectiveness. The aim of this study was firstly the chemical analysis and the antimicrobial evaluation of the EOs of Origanum onites L. and Satureja thymbra L. obtained from Symi island (Greece), and, secondly, the formulation of propylene glycol-nanovesicles loaded with these EOs to improve their antimicrobial properties. The EOs were analyzed by GC-MS and their chemical contents are presented herein. Different nanovesicles were formulated with small average sizes, high homogeneity, and optimal ζ-potential. Microscopic observation confirmed their small and spherical shape. Antibacterial and antifungal activities of the formulated EOs were evaluated against food-borne pathogens and spoilage microorganisms compared to pure EOs. Propylene glycol-nanovesicles loaded with O. onites EO were found to be the most active formulation against all tested strains. Additionally, in vitro studies on the HaCaT cell line showed that nanovesicles encapsulated with EOs had no toxic effect. The present study revealed that both EOs can be used as alternative sanitizers and preservatives in the food industry, and that their formulation in nanovesicles can provide a suitable approach as food-grade delivery system.

scholarly journals Development and Evaluation of Rifampicin Loaded Alginate–Gelatin Biocomposite Microfibers

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1514
Author(s):  
Ameya Sharma ◽  
Vivek Puri ◽  
Pradeep Kumar ◽  
Inderbir Singh ◽  
Kampanart Huanbutta
Keyword(s):  
Wound Healing ◽  
Xanthan Gum ◽  
In Vitro Release ◽  
Antimicrobial Properties ◽  
Entrapment Efficiency ◽  
Tissue Proliferation ◽  
Naturally Occurring ◽  
Reinforcing Material ◽  
Vitro Release

Various systematic phases such as inflammation, tissue proliferation, and phases of remodeling characterize the process of wound healing. The natural matrix system is suggested to maintain and escalate these phases, and for that, microfibers were fabricated employing naturally occurring polymers (biopolymers) such as sodium alginate, gelatin and xanthan gum, and reinforcing material such as nanoclay was selected. The fabrication of fibers was executed with the aid of extrusion-gelation method. Rifampicin, an antibiotic, has been incorporated into a biopolymeric solution. RF1, RF2, RF3, RF4 and RF5 were coded as various formulation batches of microfibers. The microfibers were further characterized by different techniques such as SEM, DSC, XRD, and FTIR. Mechanical properties and physical evaluations such as entrapment efficiency, water uptake and in vitro release were also carried out to explain the comparative understanding of the formulation developed. The antimicrobial activity and whole blood clotting of fabricated fibers were additionally executed, hence they showed significant results, having excellent antimicrobial properties; they could be prominent carriers for wound healing applications.

scholarly journals Antimicrobial Activity of Curcumin in Nanoformulations: A Comprehensive Review

2021 ◽  
Vol 22 (13) ◽  
pp. 7130
Author(s):  
Jeffersson Krishan Trigo-Gutierrez ◽  
Yuliana Vega-Chacón ◽  
Amanda Brandão Soares ◽  
Ewerton Garcia de Oliveira Mima
Keyword(s):  
Polymeric Nanoparticles ◽  
Graphene Quantum Dots ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Clinical Scenarios ◽  
Low Cytotoxicity ◽  
Bacteria And Fungi ◽  
Drug Resistant Strains

Curcumin (CUR) is a natural substance extracted from turmeric that has antimicrobial properties. Due to its ability to absorb light in the blue spectrum, CUR is also used as a photosensitizer (PS) in antimicrobial Photodynamic Therapy (aPDT). However, CUR is hydrophobic, unstable in solutions, and has low bioavailability, which hinders its clinical use. To circumvent these drawbacks, drug delivery systems (DDSs) have been used. In this review, we summarize the DDSs used to carry CUR and their antimicrobial effect against viruses, bacteria, and fungi, including drug-resistant strains and emergent pathogens such as SARS-CoV-2. The reviewed DDSs include colloidal (micelles, liposomes, nanoemulsions, cyclodextrins, chitosan, and other polymeric nanoparticles), metallic, and mesoporous particles, as well as graphene, quantum dots, and hybrid nanosystems such as films and hydrogels. Free (non-encapsulated) CUR and CUR loaded in DDSs have a broad-spectrum antimicrobial action when used alone or as a PS in aPDT. They also show low cytotoxicity, in vivo biocompatibility, and improved wound healing. Although there are several in vitro and some in vivo investigations describing the nanotechnological aspects and the potential antimicrobial application of CUR-loaded DDSs, clinical trials are not reported and further studies should translate this evidence to the clinical scenarios of infections.

scholarly journals Evaluating the Anti-proliferative Effects of Nanoemulsion Containing Licorice Extract and Lavender Essential Oil on Cancer

2021 ◽  
Vol 24 (1) ◽  
pp. 84-97
Author(s):  
Zohreh Karimi Taheri ◽  
◽  
Mohammad Hosein Aarabi ◽  
Ali Nazari Alam ◽  
Majid Nejati ◽  
...  
Keyword(s):  
Essential Oil ◽  
Cell Lines ◽  
Staphylococcus Epidermidis ◽  
Colorimetric Method ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Drug Formulation ◽  
Study Results ◽  
Licorice Extract

Background and Aim: Despite the anti-cancer and antimicrobial properties of licorice extract and lavender essential oil, some factors, such as low bioavailability and biodegradable, limit their therapeutic use. Using nanoparticles is a method to overcome these restrictions. This study aimed to investigate the anti-proliferative effects of nanoemulsion containing licorice extract and lavender essential oil on cancer cells; we also evaluated its antimicrobial properties in vitro. Methods & Materials: In this experimental study, nanoemulsions, containing licorice extract and lavender essential oil were developed by the spontaneous emulsion method. The anti-proliferative effect of nanoemulsion was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetric method on two cell lines HepG2 and SK-MEL-3. To measure the antimicrobial effect of 4 standard strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Minimum Inhibitory Concentration (MIC) method was used. Ethical Considerations: This study was approved by the Ethics Committee of Kashan University of Medical Sciences (Code: IR.KAUMS.MEDNT.REC.1396.106). Results: The results of MTT test on HepG2 cells indicated that the concentrations of 630, 1250, and 2500 μg/mL nanoemulsions caused toxicity to the cell and led to the death of >50% of the cells (IC50=401μg/mL; P<0.05). Evaluating SK-MEL3 cells revealed that except for 75 μg of nanoemulsion, other concentrations induced death in >50% of the cells (IC50 = 82 μg/mL; P<0.05). In addition, nanoemulsions, with antimicrobial properties, were studied in 4 strains of bacteria; the highest antimicrobial properties were observed in Staphylococcus epidermidis. Conclusion: Nanoemulsion containing licorice extract and lavender essential oil presents antimicrobial and antiproliferative effects on the two cell lines studied. The current study results indicated that the nano emulsification of lavender essential oil and licorice extract can enhance their biological impact; thus, they can be used as a drug formulation.

scholarly journals Antimicrobial Efficacy of Fruit Peels Eco-Enzyme against Enterococcus faecalis: An In Vitro Study

2020 ◽  
Vol 17 (14) ◽  
pp. 5107
Author(s):  
Hetal Ashvin Kumar Mavani ◽  
In Meei Tew ◽  
Lishen Wong ◽  
Hsu Zenn Yew ◽  
Alida Mahyuddin ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Antimicrobial Efficacy ◽  
Significant Difference ◽  
Potential Alternative ◽  
The One

Sodium hypochlorite (NaOCl), an effective endodontic irrigant against Enterococcus faecalis (EF), is harmful to periapical tissues. Natural pineapple-orange eco-enzymes (M-EE) and papaya eco-enzyme (P-EE) could be potential alternatives. This study aimed to assess the antimicrobial efficacy of M-EE and P-EE at different concentrations and fermentation periods against EF, compared to 2.5% NaOCl. Fermented M-EE and P-EE (3 and 6 months) at various concentrations were mixed with EF in a 96-well plate incubated for 24 h anaerobically. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of M-EE and P-EE were determined via EF growth observation. EF inhibition was quantitatively measured and compared between different irrigants using the one-way analysis of variance (ANOVA), and different fermentation periods using the independent-samples T-test. M-EE and P-EE showed MIC at 50% and MBC at 100% concentrations. There was no significant difference in antimicrobial effect when comparing M-EE and P-EE at 50% and 100% to 2.5% NaOCl. P-EE at 6 months fermentation exhibited higher EF inhibition compared to 3 months at concentrations of 25% (p = 0.017) and 0.78% (p = 0.009). The antimicrobial properties of M-EE and P-EE, at both 100% and 50% concentrations, are comparable to 2.5% NaOCl. They could therefore be potential alternative endodontic irrigants, but further studies are required.

scholarly journals Natural Phenolic Compounds for the Control of Oxidation, Bacterial Spoilage, and Foodborne Pathogens in Meat

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 794 ◽  
Author(s):  
Aphrodite I. Kalogianni ◽  
Thomai Lazou ◽  
Ioannis Bossis ◽  
Athanasios I. Gelasakis
Keyword(s):  
Phenolic Compounds ◽  
Foodborne Pathogens ◽  
Current Knowledge ◽  
Antimicrobial Properties ◽  
Meat Industry ◽  
Beneficial Effects ◽  
Naturally Occurring ◽  
Antioxidant And Antimicrobial Activity ◽  
Relevant Regulation

Alternative technologies for long-term preservation, quality assurance, and safety of meat are continuously pursued by the food industry to satisfy the demands of modern consumers for nutritious and healthy meat-based products. Naturally occurring phenolic compounds are considered promising substances by the meat industry for their antioxidant and antimicrobial properties, while consumers seem to embrace them for their claimed health benefits. Despite the numerous in vitro and in situ studies demonstrating their beneficial effects against meat oxidation, spoilage, and foodborne pathogens, wide application and commercialization has not been yet achieved. Major obstacles are still the scarcity of legislative framework, the large variety of meat-based products and targeted pathogens, the limited number of case-specific application protocols and the questionable universal efficiency of the applied ones. The objectives of the present review are (i) to summarize the current knowledge about the applications of naturally occurring phenols in meat and meat-based products, emphasizing the mechanisms, determinants, and spectrum of their antioxidant and antimicrobial activity; (ii) to present state-of-the-art technologies utilized for the application of phenolic compounds in meat systems; and (iii) to discuss relevant regulation, limitations, perspectives, and future challenges for their mass industrial use.

scholarly journals INVESTIGATION OF ANTIMICROBIAL EFFECT OF DECASAN CHLORHEXIDINE AND IODOPHORAM ON STANDARD MICROBIAL STRAINS

2021 ◽  
Vol 21 (3) ◽  
pp. 190-195
Author(s):  
M.M. Ananieva ◽  
Y.V. Chumak ◽  
G.A. Loban ◽  
M.O. Faustova
Keyword(s):  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Crystalline Substance ◽  
Bacterial Cells ◽  
Tooth Socket ◽  
Treatment And Prevention ◽  
Esherichia Coli ◽  
Microbial Strains

The problem of treatment and prevention of infectious-inflammatory post extraction complications in modern surgical dentistry are still challenging, despite the great advances in this field. Many etiological factors are identified as contributing to the development of such complications, but at present scientists are paying much attention to the infectious factor, which is associated with the microflora of the tooth socket after tooth removal. According to the literature, the most common post-extraction complication during an outpatient surgical appointment is alveolitis, which accounts for 1-35% of all cases of tooth extraction. To combat an infectious etiological factor of alveolitis, dental surgeons apply topical antiseptics with a broad spectrum of action such as iodoform and chlorhexidine. As a topical antiseptic drug in the treatment and prevention of oral infectious and inflammatory complications, a domestic antiseptic, Decasan, based on decamethoxine, is becoming more widely applied. Previous studies have shown that this antiseptic has antimicrobial effect against gram-positive and gram-negative, aerobic and anaerobic bacteria; this requires further in-depth studies of its properties, which could be applied in the dental surgical practice to treat and prevent infectious-inflammatory post extraction complications. Chlorhexidine bigluconate is a cationic biguanide. Penetrating into the intracellular membranes of bacteria, it impedes the oxygen consumption and leads to the death of bacterial cells. This antiseptic is widely used in dental practice. Treatment of alveolitis is carried out by using the standard method, namely by using iodoform tamponade of the tooth socket in combination with other medicines. Iodoform (triiodomethane) is a yellow crystalline substance with a strong characteristic odor, practically insoluble in water. In dentistry, this drug is used as an antiseptic in powder form, or combined pastes. The purpose of this in vitro study is to investigate the antimicrobial properties of Decasan, chlorhexidine and iodoform against museum microbial strains. Materials and methods. Museum strains of Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 14990, Enterococcus faecalis ATCC 29212, Esherichia coli ATCC 25922, Candida albicans ATCC 10231, obtained from L.V. Gromashevsky Institute of Epidemiology and Infectious Diseases, National Academy of Medical Sciences of Ukraine, were used as studied cultures of microorganisms. The antimicrobial effect of decasan, chlorhexidine and iodoform was assessed by quantitative method of serial dilutions in broth and agar according to the order №167, dated 5.04.2007 On approval of guidelines “Determination of sensitivity of microorganisms to antibacterial drugs". Conclusion. According to the data obtained, decasan and chlorhexidine demonstrate higher bacteriostatic and bactericidal effect on the studied museum microbial strains at a concentration thousands of times lower than the concentration of iodoform.

In Vitro Antimicrobial Properties of Propolis against Caries-Associated Microorganisms

2015 ◽  
Vol 752-753 ◽  
pp. 387-390
Author(s):  
Boonyanit Thaweboon ◽  
Sroisiri Thaweboon ◽  
Rattiporn Kaypetch ◽  
Thaniya Muadcheingka
Keyword(s):  
Antimicrobial Activity ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Oral Care ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Scientific Basis ◽  
Agar Disc ◽  
Zones Of Inhibition

Natural products have attracted much attention in recent years as a useful substance used in medicine and dental products. Propolis is a lipophilic resinous mixture collected from various plant sources by honeybees. It has been known for many biological activities including antimicrobial properties. Objective: To evaluate thein vitroantimicrobial activity of propolis against caries-associated microorganisms,Streptococcusmutans,LactobacilluscaseiandCandidaalbicans. Materials and methods: The antimicrobial activity was initially screened using agar disc diffusion and then the minimum lethal concentrations (MLCs) were determined using broth dilution technique. Chlorhexidine (0.2%) and dimethyl sulfoxide (5%) were used as positive and negative controls, respectively. Results: Propolis exhibits the zones of inhibition ranged from 15 to 18 mm forS.mutans, and from 10 to 12 mm forL.casei, but presented no activity againstC.albicans. The MLC againstS.mutanswas 2.86 mg/mL and those againstL.caseiwere 11.44 mg/mL and 12.87 mg/mL. Conclusion: Propolis shows a potent antimicrobial effect againstS.mutansandL.casei.This could provide a scientific basis to its application of usage in oral care products for the prevention of dental caries. However, further studies are necessary to evaluate other biological activities and molecular mechanisms of action as well as its cytotoxic effect.

scholarly journals Antimicrobial Properties of Strontium Functionalized Titanium Surfaces for Oral Applications, A Systematic Review

Coatings ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 810
Author(s):  
Hatem Alshammari ◽  
Fahad Bakitian ◽  
Jessica Neilands ◽  
Ole Zoffmann Andersen ◽  
Andreas Stavropoulos
Keyword(s):  
Systematic Review ◽  
Scientific Evidence ◽  
Statistical Significance ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Ion Release ◽  
Biofilm Inhibition ◽  
E Coli ◽  
Antimicrobial Potential

The aim of this systematic review was to assess the current scientific evidence of the antimicrobial potential of strontium (Sr) when used to functionalize titanium (Ti) for oral applications. Out of an initial list of 1081 potentially relevant publications identified in three electronic databases (MEDLINE via PubMed, Scopus, and Cochrane) up to 1 February 2021, nine publications based on in vitro studies met the inclusion criteria. The antimicrobial potential of Sr was investigated on different types of functionalized Ti substrates, employing different application methods. Nine studies reported on the early, i.e., 6–24 h, and two studies on the late, i.e., 7–28 days, antimicrobial effect of Sr, primarily against Staphylococcus aureus (S. aureus) and/or Escherichia coli (E. coli). Sr-modified samples demonstrated relevant early antimicrobial potential against S. aureus in three studies; only one of which presented statistical significance values, while the other two presented only the percentage of antimicrobial rate and biofilm inhibition. A relevant late biofilm inhibition potential against S. aureus of 40% and 10%—after 7 and 14 days, respectively—was reported in one study. Combining Sr with other metal ions, i.e., silver (Ag), zinc (Zn), and fluorine (F), demonstrated a significant antimicrobial effect and biofilm inhibition against both S. aureus and E. coli. Sr ion release within the first 24 h was generally low, i.e., below 50 µg/L and 0.6 ppm; however, sustained Sr ion release for up to 30 days, while maintaining up to 90% of its original content, was also demonstrated. Thus, in most studies included herein, Sr-functionalized Ti showed a limited immediate (i.e., 24 h) antimicrobial effect, likely due to a low Sr ion release; however, with an adequate Sr ion release, a relevant antimicrobial effect, as well as a biofilm inhibition potential against S. aureus—but not E. coli—was observed at both early and late timepoints. Future studies should assess the antimicrobial potential of Ti functionalized with Sr against multispecies biofilms associated with peri-implantitis.

scholarly journals In-Vitro Antimicrobial Effect of Aqueous and Alcoholic Extracts of Rheum Ribes on Some Food-Borne Pathogens

2016 ◽  
Vol 23 (3) ◽  
pp. 430-437
Author(s):  
Ali Salehi ◽  
Nabi Shariatifar ◽  
Aftab Salehi ◽  
Alireza Mohammadzadeh
Keyword(s):  
Antimicrobial Effect ◽  
Food Borne Pathogens ◽  
Food Borne
Sign in / Sign up

Export Citation Format

Share Document