scholarly journals Core-shell in liquid chromatography: application for determining sulphonamides in feed and meat using conventional chromatographic systems

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Antonio Armentano ◽  
Simona Summa ◽  
Sonia Lo Magro ◽  
Pasquale D’Antini ◽  
Carmen Palermo ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Acetate Buffer Solution ◽  
Array Detector ◽  
Core Shell ◽  
Good Resolution ◽  
Buffer Solution ◽  
Binary Mobile Phase ◽  
Shell Particles ◽  
Gradient Mode

A C18 column packed with core-shell particles was used for the chromatographic separation of sulphonamides in feed and meat by a conventional high performance liquid chromatography system coupled with a diode array detector. Two analytical methods, already used in our laboratory, have been modified without any changes in the extraction and clean-up steps and in the liquid chromatography instrumentation. Chromatographic conditions applied on a traditional 5-μm column have been optimized on a column packed with 2.6 μm core-shell particles. A binary mobile phase [acetate buffer solution at pH 4.50 and a mixture of methanol acetonitrile 50: 50 (v/v)] was employed in gradient mode at the flow rate of 1.2 mL with an injection volume of 6 μL. These chromatographic conditions allow the separation of 13 sulphonamides with an entire run of 13 minutes. Preliminary studies have been carried out comparing blanks and spiked samples of feed and meat. A good resolution and the absence of interferences were achieved in chromatograms for both matrices. Since no change was made to the sample preparation, the optimized method does not require a complete revalidation and can be used to make routine analysis faster.

scholarly journals Core-Shell Columns in High-Performance Liquid Chromatography: Food Analysis Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Raffaella Preti
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Food Analysis ◽  
High Performance ◽  
Separation Efficiency ◽  
New Technology ◽  
Core Shell ◽  
Shell Particles

The increased separation efficiency provided by the new technology of column packed with core-shell particles in high-performance liquid chromatography (HPLC) has resulted in their widespread diffusion in several analytical fields: from pharmaceutical, biological, environmental, and toxicological. The present paper presents their most recent applications in food analysis. Their use has proved to be particularly advantageous for the determination of compounds at trace levels or when a large amount of samples must be analyzed fast using reliable and solvent-saving apparatus. The literature hereby described shows how the outstanding performances provided by core-shell particles column on a traditional HPLC instruments are comparable to those obtained with a costly UHPLC instrumentation, making this novel column a promising key tool in food analysis.

Pushing the performance limits of reversed-phase ultra high performance liquid chromatography with 1.3μm core–shell particles

2013 ◽  
Vol 1311 ◽  
pp. 90-97 ◽  
Author(s):  
A. Carl Sanchez ◽  
Gareth Friedlander ◽  
Szabolcs Fekete ◽  
Jason Anspach ◽  
Dvy Guillarme ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Core Shell ◽  
Performance Limits ◽  
Shell Particles

scholarly journals Theoretical Analysis of Efficiency of Multi-Layer Core-Shell Stationary Phases in the High Performance Liquid Chromatography of Large Biomolecules

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2849 ◽  
Author(s):  
Szabolcs Horváth ◽  
Fabrice Gritti ◽  
Róbert Kormány ◽  
Krisztián Horváth
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Layer Structure ◽  
Stationary Phases ◽  
Particle Diameter ◽  
General Purpose ◽  
Core Shell ◽  
Core Size ◽  
Particle Structure ◽  
Shell Particles

Modern analytical applications of liquid chromatography require columns with higher and higher efficiencies. In this work, the general rate model (GRM) of chromatography is used for the analysis of the efficiency of core-shell phases having two porous layers with different structures and/or surface chemistries. The solution of the GRM in the Laplace domain allows for the calculation of moments of elution curves (retention time and peak width), which are used for the analysis of the efficiency of bi-layer particles with and without a non-porous core. The results demonstrate that bi-layer structures can offer higher separation power than that of the two layers alone if the inner layer has smaller surface coverage (retentivity) and the pore size and pore diffusion of the outer layer is either equal to or higher than that of the inner layer. Even in the case of core-shell phases, there is an increase in resolution by applying the bi-layer structure; however, we can always find a mono-layer core-shell particle structure with a larger core size that provides better resolution. At the optimal core size, the resolution cannot be further improved by applying a bi-layer structure. However, in case of the most widely produced general-purpose core-shell particles, where the core is ∼70% of the particle diameter, a 15–20% gain of resolution can be obtained by using well-designed and optimized bi-layer core-shell phases.

Determination of residual dimethylaminopropylamine in aminopropylamides of fatty acid using high-performance liquid chromatography

2021 ◽  
Vol 87 (4) ◽  
pp. 21-25
Author(s):  
D. A. Krasnikov ◽  
I. V. Demchak ◽  
E. S. Khudoleeva
Keyword(s):  
Fatty Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Sulfonyl Chloride ◽  
Phosphate Buffer Solution ◽  
Array Detector ◽  
Precolumn Derivatization ◽  
Buffer Solution

A procedure for determination of the residual content of N,N-dimethyl-1,3-propanediamine (DMAPA) during synthesis of N,N-dimethylaminopropylamides of fatty acids (DMAPA FA) from coconut oil has been developed. The analysis was performed using high performance liquid chromatography on a Shimadzu LC-20 Prominence device equipped with a diode array detector and reversed phase column GL Sciences Inertsil ODS-3. Precolumn derivatization was carried out with a 13% — dansyl chloride (5-(dimethylamino)naphthalene-1-sulfonyl chloride) solution in acetone to increase the analyte response. The selected composition of the mobile phase — acetonitrile and triethylamine phosphate buffer solution (pH 3.0) in a volume ratio of 2:3 and optimized chromatographic conditions provided a clear peak of DMAPA with a retention time of 8.7 – 8.9 min. The proposed method provides determination of 0.02 – 10% DMAPA in DMAPAFA samples. Correctness of the procedure was confirmed in spike tests. The results obtained can be used for assessing the degree of conversion of starting materials in the synthesis of N,N-dimethylaminopropylamides of fatty acids, as well as for forecasting the content of N,N-dimethyl-1,3-propanediamine in the products on their base.

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