scholarly journals Aflatoxin M1 levels in raw milk, pasteurized milk and infant formula

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Sharaf Shareef Omar
Keyword(s):  
European Union ◽  
Infant Formula ◽  
Raw Milk ◽  
Average Concentration ◽  
Tolerance Limit ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk Samples ◽  
Maximum Tolerance

The incidence of contamination of aflatoxin M1 (AFM1) in milk samples collected from the Jordanian market was investigated by using the competitive enzyme linked immunosorbent assay (ELISA) technique. A total of 175 samples were collected during 2014-2015 year. All tested samples were contaminated with various levels of AFM1 ranging from 9.71 to 288.68 ng/kg. The concentration of AFM1 in 66% of fresh milk samples was higher than the maximum tolerance limit accepted by European Union (50 ng/kg) and 23% higher than the maximum tolerance limit accepted by USA (500 ng/kg). Percentages of contaminated raw cow, sheep, goat and camel milk exceeding the European tolerance limit were 60, 85, 75 and 0%, respectively. Twelve % of AFM1 contaminated pasteurized cow milk samples exceeded the European tolerance limit with a range of contamination between 14.60 and 216.78 ng/kg. For infant formula samples, the average concentration of AFM1 was 120.26 ng/kg (range between 16.55 to 288.68 ng/kg), the concentration of AFM1 in 85% of infant formula samples were higher than the maximum tolerance limit accepted by European Union and USA (25 ng/kg).

scholarly journals Determination of Aflatoxin M1 in Milk by ELISA Technique in Mashad (Northeast of Iran)

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Ali Mohamadi Sani ◽  
Mohamad Khezri ◽  
Halime Moradnia
Keyword(s):  
Average Concentration ◽  
Tolerance Limit ◽  
National Standard ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Contamination Level ◽  
Milk Samples ◽  
Maximum Tolerance ◽  
Elisa Technique

The aim of this study was to detect the amount of aflatoxin M1 (AFM1) in pasteurized milk samples in Mashad in northeast of Iran. For this purpose, 42 milk samples were collected from retail stores during fall 2011 and analyzed for AFM1 by enzyme-linked immunosorbent assay (ELISA) technique. All the analyses were done twice. Results showed presence of AFM1 in 97.6% of the examined milk samples by average concentration of 23 ± 16 ppt and contamination level ranging between 6 and 71 ppt. The concentration of AFM1 in all the samples was lower than the Iranian national standard and Food and Drug Administration limits (500 ppt), and, only in 3 (1.6%) samples, AFM1 concentration was more than the maximum tolerance limit (50 ppt) accepted by European Union and Codex Alimentarius Commission. According to our findings and previous studies, AFM1 contamination of milk is not a concern in this region, and the regional standard of AFM1 contamination in milk might be changed to lower than 100 ppt.

scholarly journals Study of aflatoxins incidence in cow feed and milk in Serbia during 2013.

2015 ◽  
Vol 69 (6) ◽  
pp. 651-656 ◽  
Author(s):  
Danka Spiric ◽  
Jasna Djinovic ◽  
Vesna Jankovic ◽  
Branko Velebit ◽  
Tatjana Radicevic ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Aflatoxin B1 ◽  
Negative Impact ◽  
Raw Milk ◽  
Self Control ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Climate Conditions ◽  
Milk Samples ◽  
Beet Pulp

Atypical weather and climate conditions during the spring and summer 2012th were assumed to be the main reason for the aflatoxins contamination of corn crops in Serbia. High humidity in spring, and summer temperatures above the average contributed to the increased possibility of mycotoxins occurance in cereals in the fields. As a consequence, at the begining of 2013th contaminated corn used for dairy cows diet had negative impact on the safety of cow milk. The routine laboratory control data revealed an increased content of aflatoxin M1 in milk samples. Large number of raw milk and various feedstuff samples were collected from February to April 2013th and were analysed for the presence of aflatoxin M1 (AFM1) and aflatoxin B1 respectively. The collected samples were a part of enhanced self-control plans of the large dairy farms. Quantitative competitive and sandwich types of ELISA tests were used for the screening analysis of the feed and milk samples. Confirmation of the positive results obtained by ELISA tests was performed by UPLC-MS/MS method. Out of 281 samples of complete mixtures for dairy cows, 67 samples (24%) contained aflatoxin B1 quantities higher than the MRL of 0,005 mg/kg [4]. Corn samples were also tested for the presence of aflatoxin B1 revealing contamination of 22% above the MRL (0.03mg/kg). Aflatoxin M1 content in the 934 positive milk samples ranged from 0,005- 1,25 ?g/kg. The corresponding feed samples of sunflower meal, hay silage, corn silage and sugar beet pulp were screening negative, with the content of aflatoxin B1 less than 2 ?g/kg. The main source of aflatoxins were corn samples, wholemeal and feed mixtures derived from contaminated corn. The contamination peak was during March 2013, when 65% of milk samples contained amounts of aflatoxin M1 higher than 0,05 ?g/kg, and 13% of milk samples contained amounts higher than 0,5 ?g/kg.

scholarly journals Occurrence and seasonal variation of aflatoxin M1 in raw cow milk collected from different regions of Algeria

2020 ◽  
Vol 13 (3) ◽  
pp. 433-439 ◽  
Author(s):  
Sarah Mohammedi-Ameur ◽  
Mohammedi Dahmane ◽  
Carlo Brera ◽  
Moustafa Kardjadj ◽  
Meriem Hind Ben-Mahdi
Keyword(s):  
Animal Feed ◽  
Control Program ◽  
Raw Milk ◽  
Aflatoxin Contamination ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Survey Period ◽  
Lactating Cows ◽  
The Usa

Background and Aim: Aflatoxins are metabolites of molds that exert potentially toxic effect on animals and humans. This study aimed to investigate the occurrence of aflatoxin M1 (AFM1) in raw cow milk collected during 1 year (2016-2017) from different regions of Algeria and risk factors associated with the contamination. Materials and Methods: During the survey period, 84 samples of raw milk were collected in three regions of Algeria (northeast, north center, and northwest) during four seasons. AFM1 levels were analyzed by competitive enzyme-linked immunosorbent assay. Results: AFM1 was detected in 39 (46.43%) samples (total mean concentration, 71.92 ng/L; range, 95.59-557.22 ng/L). However, the AFM1 levels exceeded the maximum tolerance limit set by the Food and Drug Administration in the USA (500 ng/L) in only 1 sample (1.19%). Statistical analysis revealed significant differences (p<0.005) between AFM1 levels in milk samples collected in the spring and autumn. The mean AFM1 levels in samples collected in the spring were significantly higher than those in samples collected in autumn. Conclusion: The survey indicates that farmers involved in milk production should be made aware of the adverse effects of aflatoxin contamination in animal feed. A systematic control program of supplementary feedstuff for lactating cows should be introduced by the public health authorities.

A Study on the Presence of Aflatoxin M1 in Cow’s Milk in Jiroft

2021 ◽  
Author(s):  
Mohadeseh Kamali ◽  
Seyyedeh Sedigheh Seyyedi ◽  
Mehdi Taheri Sarvtin
Keyword(s):  
Immune Deficiency ◽  
Chemical Form ◽  
Aflatoxin M1 ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
The European Union ◽  
Cow's Milk ◽  
Tolerance Level ◽  
Milk Samples ◽  
Maximum Tolerance

Background and Aims: Cow's milk is a daily staple food for many individuals that can be contaminated with many toxins such as aflatoxin M1 (AFM1). AFM1 is a chemical form of the aflatoxin B1 produced by some species of Aspergillus genus like A. ochraceus, A. flavus, A. nomius, and A. parasiticus that can contaminate feed and forage cattle. This toxin enters into the milk after eating contaminated feed by cows. AFM1 can cause various dangerous diseases such as cancer and immune deficiency in humans. The present study is aimed to investigate the level of AFM1 in cow's milk in Jiroft, Kerman Province, Iran. Materials and Methods: A total of 90 cow’s milk samples were collected in spring and summer 2019 from available stores in Jiroft city. Enzyme-linked immunosorbent assay was used to measure AFM1 in all cow’s milk samples.Results: In the present study, AFM1 was found in 88 (97.8%) milk samples with a range of 0.2-90.62 ppt (mean, 20.07±24.46 ppt). AFM1 concentrations exceeded 50 ppt (maximum tolerance level of AFM1 in the European Union) was seen in 12 (13.3%) samples. Conclusions: The results of this study showed the presence of AFM1 in cow's milk in Jiroft city. So, in this region, many people are exposed to dangerous diseases such as cancer due to the consumption of milk contaminated with AFM1.

scholarly journals Study on the Level of Aflatoxin M1 Contamination in Raw and Processed Milk Marketed in Kathmandu Valley

2014 ◽  
Vol 7 ◽  
pp. 52-56 ◽  
Author(s):  
Pratap Kafle ◽  
Damodar Sedai ◽  
Krishna Prasad Rai ◽  
Bishow Bandhu Pokharel
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Raw Milk ◽  
Aflatoxin M1 ◽  
Kathmandu Valley ◽  
Milk Samples ◽  
Residual Level ◽  
Maximum Tolerance ◽  
The Difference ◽  
Layer Chromatography

Aflatoxin M1 (AFM1) is the principal hydroxylated AFB1 metabolite mainly present in milk. In this study the levels of Aflatoxin M1 (AFM1) in Raw and Pasteurized milk marketed in Kathmandu valley was estimated. Altogether 32 milk samples (Raw 16, Pasteurized 16) obtained from different areas of Kathmandu valley were analysed for AFM1 by Thin Layer Chromatography. The milk samples were analyzed according to the official AOAC methods, which included extraction of toxin using chloroform, clearing by silica gel column chromatography, qualitative analysis by Thin Layer Chromatography and quantification by Visual comparison of the spots. AFM1 was found in 14 (43.75%) of milk samples examined. The levels of AFM1 in 7 (21.87%) samples were higher than the maximum tolerance limit (0.05 µg/l) accepted by some European countries while none of the samples exceeded the prescribed limit of US regulations. The mean concentration of AFM1 was higher in Raw milk (0.030 ± 0.042 µg/l) compared to pasteurized (0.022 ± 0.039) but the difference was not statistically significant (p>0.05). This finding reflects that milk marketed in Kathmandu valley contains residual level of Aflatoxin M1 and pose public health risk. Therefore, milk and milk products have to be screened for AFM1 contamination periodically. DOI: http://dx.doi.org/10.3126/jfstn.v7i0.10598   J. Food Sci. Technol. Nepal, Vol. 7 (52-56), 2012  

Occurrence of Aflatoxin in Dairy Cow Feed Ingredients and Total Mixed Ration

2019 ◽  
Vol 35 (5) ◽  
pp. 679-686 ◽  
Author(s):  
Zeinab Mohammadi Shad ◽  
Mehrdad Ghavami ◽  
Griffiths G. Atungulu
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Rainy Season ◽  
Raw Milk ◽  
Farm Management ◽  
Average Level ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Feed Ingredients ◽  
Milk Samples

Abstract. The level of aflatoxin B1 (AFB1) in dairy cow feed ingredients and Total Mixed Rations (TMRs) procured at two farms for low- and high-yielding dairy cows were surveyed. Raw milk from the two groups of cows at each farm was sampled 24 h after feeding the cows with examined feedstuffs during both the rainy and the non-rainy season. The aflatoxin M1 (AFM1) level in the raw milk samples was measured 12-24 h later. The levels of AFB1 in feed and AFM1 in milk were determined by validated enzyme linked immunosorbent assay (ELISA). The influence of farm management and type of feeding system on aflatoxin occurrence were considered. AFB1 and AFM1 were detected in 100% of feed and milk samples, respectively. The average level of AFB1 in the feed ingredients and TMRs were in the range of 1.6-104.7 µg/kg and 11.0-56.0 µg/kg, respectively. The average level of AFM1 in milk samples was 77.0 ng/L. The average concentrations of AFB1 in feeds and AFM1 in milk procured in the rainy season were significantly greater than those procured in the non-rainy season (p&lt;0.05). Of the studied feed, maize silage was determined as the most contaminated feed ingredient in terms of AFB1 content. Furthermore, the AFM1 in 75% of milk samples obtained from high-yielding dairy cows and 25% of milk samples obtained from low-yielding dairy cows indicated AFM1 level higher than the maximum allowable Europe Commission limit of 50 ng/L. The results also showed that the occurrence of AFB1 in feed varied with farm feed management. The extent of translocation to AFMI in milk samples was dependent on type of cow, whether low- or high- milk yielding. This study suggests regular risk analysis and using good farm management practices are important to control aflatoxin contamination in feed and milk. Keywords: Aflatoxin B1, Aflatoxin M1, Dairy cows, Feed, Milk yield.

scholarly journals Presence of moulds and aflatoxin M1 in milk

2009 ◽  
pp. 63-68 ◽  
Author(s):  
Vesna Jankovic ◽  
Jelena Vukojevic ◽  
Brankica Lakicevic ◽  
Radmila Mitrovic ◽  
Dejan Vukovic
Keyword(s):  
Dairy Products ◽  
Raw Milk ◽  
Direct Result ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Contamination Level ◽  
Infant Food ◽  
Fungal Contamination ◽  
Milk Samples ◽  
Feed Samples

Aflatoxin M1 (AFM1) appears in milk or dairy products as a direct result of the cattle's ingestion of feed contaminated with aflatoxin B1 (AFB1). This study comprises mycological and mycotoxicological investigations of 23 milk samples (raw, infant food, pasteurized, whey and yoghurt). The mycological testing showed dominant presence of genus Geotrichum. G. candidum was found in 9 samples, with the highest contamination in the raw milk samples. The contamination level of AM1 is defined by using direct competitive enzyme- -linked immunosorbent assay (ELISA). AFM1 was found in 9 samples. AFM1 levels were lower than the recommended limits. However, as AFM1 is considered a probable human carcinogen (2B type), it is necessary to achieve a low level of AFM1 in milk. Therefore, cows' feed samples from various cowsheds are supposed to be evaluated routinely for aflatoxin, and kept away from fungal contamination as much as possible.

scholarly journals Aflatoxin M1 Contamination in Raw Cow Milk of Dairy Farms in Behbahan area, Khuzestan province, Iran

2019 ◽  
Author(s):  
Seyedeh Ommolbanin Ghasemian
Keyword(s):  
Dairy Farms ◽  
Raw Milk ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Natural Occurrence ◽  
Environmental Chemical ◽  
Khuzestan Province ◽  
Milk Samples ◽  
Significant Difference ◽  
Southwest Of Iran

Introduction: Mycotoxins are among the environmental chemical agents with toxic effects on animals and humans. Aflatoxins (AF) are a major group of mycotoxins produced following the growth of some species of Aspergillus flavus and Aspergillus parasiticus, particularly in agricultural products. Aflatoxin M1 (AFM1) is secreted to milk and remains there since the process of pasteurization and sterilization has little effect on this metabolite. The aim of this study was to investigate the natural occurrence of AFM1 in raw milk in Behbahan area, which is an area of high-density livestock production in southwest of Iran. The other aim was to compare these obtained levels with the accepted level recommended by the European community (50 ng/L). Methods: In the present study, 90 samples of raw milk were collected from five dairy farms in Behbahan region, Khuzestan province, Iran. The AFM1 was analyzed using the Enzyme Linked Immune Sorbent Assay (ELISA) method by ELISA kits from Euro Clone, Italy.  Data were analyzed by ANOVA and the results were reported as mean ± SE. The significant level was set at p ≤ 0.05.  Results: The concentration of aflatoxin M1 in (15)16.54% of raw milk was higher than maximum tolerance limit accepted by European Union /Codex Alimentarius Commission (50 ng/l). The results of this study showed that all 90 investigated raw milk samples were contaminated with AFM1 (100%). Conclusion: A significant difference was observed between concentration means of AFM1 in various studied farms at P < 0.05. Consequently, high occurrence of AFM1 in raw milk samples was considered as a possible hazard for human health. However, further longitudinal studies are required on milk samples and other dairy products in Behbahan as well as other regions of Iran to estimate the representative intake of AFM1 in Iran.

scholarly journals Screening of aflatoxin M1 in cows’ milk in Gadarif town, Sudan

2021 ◽  
Author(s):  
Kamal M. A. Abdalmahmoud ◽  
El Tahir S. Shuiep ◽  
Ibtisam E El Zubeir ◽  
Omer H. M. Arabi
Keyword(s):  
Health Risk ◽  
Screening Method ◽  
Raw Milk ◽  
Aflatoxin Contamination ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Milk Samples ◽  
Important Health ◽  
Different Sources

Abstract Background Milk is a perfect complete perishable food that could be contaminated by any substance throughout the chain of its production. Of these aflatoxins M1 was considered as an important health risk associated with the milk. The objectives of this study are to analyse milk samples for the detection of aflatoxin M1. Method Eighty milk samples were collected from different sources randomly; 33 samples from farms, 31 samples from sale points and 16 samples from groceries in Gedarif town. Unisensor kits were used as screening method for detection of aflatoxin M1 in milk. Results The occurrence of aflatoxin M1 in raw milk samples were found in 22(27.50%) of the samples. The presence of aflatoxin contamination was high in milk samples collected from sales points (15.0%) followed by farms (11.25%) compared to those obtained from groceries (1.25%). Conclusion The presences of aflatoxin M1 in the milk samples might indicate that the cow milk was contaminated with aflatoxins through feed. Hence this study recommended that good hygienic practices should be considered in Gadarif town.

scholarly journals Determination of Aflatoxin M1 in Raw Milk Using an HPLC-FL Method in Comparison with Commercial ELISA Kits—Application in Raw Milk Samples from Various Regions of Greece

2021 ◽  
Vol 8 (3) ◽  
pp. 46
Author(s):  
Martha Maggira ◽  
Maria Ioannidou ◽  
Ioannis Sakaridis ◽  
Georgios Samouris
Keyword(s):  
Limit Of Detection ◽  
Reference Method ◽  
Raw Milk ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescence Detector ◽  
Elisa Kit ◽  
Milk Samples ◽  
Of Greece

The highly toxic Aflatoxin M1 (AFM1) is most often detected in milk using an Enzyme-Linked-Immunosorbent Assay (ELISA) for screening purposes, while High-Performance Liquid Chromatography with Fluorescence Detector (HPLC-FL) is the reference method used for confirmation. The aim of the present study was the comparison between three commercially available ELISA kits and a newly developed HPLC-FL method for the determination of the AFM1 in milk samples. The developed HPLC-FL method was validated for the AFM1 and Aflatoxin M2 (AFM2), determining the accuracy, precision, linearity, decision limit, and detection capability with fairly good results. All three ELISA kits were also validated and showed equally good performance with high recovery rates. Moreover, the Limit Of Detection (LOD) and Limit Of Quantification (LOQ) values were found to be significantly lower than the Maximum Residue Limit (MRL) (50 ng kg−1). After the evaluation of all three commercial kits, the ELISA kit with the optimum performance along with the HPLC method was used for the determination of AFM1 in raw cow’s, goat’s, and sheep’s milk samples (396) obtained from producers in different regions of Greece. The evaluation of both methods showed that this ELISA kit could be considered as a faster and equally reliable alternative method to HPLC in routine analysis for the determination of AFM1 in milk.

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