scholarly journals Monitoring the freezing point of buffalo milk

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Antonella Pesce ◽  
Caterina Salzano ◽  
Anna De Felice ◽  
Francesca Garofalo ◽  
Salvatore Liguori ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Freezing Point ◽  
Bovine Milk ◽  
Buffalo Milk ◽  
Infrared Spectrometry ◽  
Milk Samples ◽  
Bulk Milk ◽  
Cattle Farms

The aim of this study was to evaluate the basic freezing point of buffalo milk. Bulk milk samples were collected from buffalo and cattle farms in Caserta area from 2008 to 2014. The analysis involved a total of 1886 buffalo milk samples and 1711 bovine milk samples. These were also tested for fat, protein and lactose contents by means of infrared spectrometry. The freezing point was determined by means of a thermistor cryoscope. Data underwent statistical analysis. Our research showed an average freezing point of -0.528°C for buffalo milk and - 0.522°C for bovine milk. Given the lack of data on the freezing point of buffalo milk, our study provides the first indication of a basic freezing point of the milk of this species in Italy.

Cryoscopy of Milk: Effect of Variations in the Method

1966 ◽  
Vol 49 (3) ◽  
pp. 511-515 ◽  
Author(s):  
R W Henningson
Keyword(s):  
Statistical Analysis ◽  
Sample Size ◽  
Freezing Point ◽  
Collaborative Study ◽  
Sample Temperature ◽  
Milk Samples ◽  
Two Samples ◽  
Temperature Rate

Abstract Bath level, sample temperature, rate of stirring, degree of supercooling, sample size, sample isolation, and refreezing of the sample were the variables in the thermistor cryoscopic method for the determination of the freezing point value of milk chosen for study. Freezing point values were determined for two samples of milk and two secondary salt standards utilizing eight combinations of the seven variables in two test patterns. The freezing point value of the salt standards ranged from –0.413 to –0.433°C and from –0.431 to –0.642°C. The freezing point values of the milk samples ranged from –0.502 to –0.544°C and from –0.518 to –0.550°C. Statistical analysis of the data showed that sample isolation was a poor procedure and that other variables produced changes in the freezing point value ranging from 0.001 to 0.011°C. It is recommended that specific directions be instituted for the thermistor cryoscopic method, 15.040–15.041, and that the method be subjected to a collaborative study.

Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India

2021 ◽  
pp. 1-10
Author(s):  
H. Thukral ◽  
P. Dhaka ◽  
J. Singh Bedi ◽  
R. Singh Aulakh
Keyword(s):  
Risk Factors ◽  
European Union ◽  
Bovine Milk ◽  
Dairy Farms ◽  
Buffalo Milk ◽  
Cow Milk ◽  
Milk Samples ◽  
Significant Difference ◽  
Associated Risk Factors ◽  
Mean Concentration

Aflatoxin M1 (AFM1) contamination in milk and milk products may pose a major public health concern. The present cross-sectional study was aimed to estimate the prevalence of AFM1 in bovine milk across all districts of Punjab, India and to identify the associated animal and farm level risk factors. A total of 402 milk samples (266 cow milk and 136 buffalo milk) were analysed using commercial ELISA and representative samples were confirmed using HPLC-FLD. The results revealed that 56.2 and 13.4% of the milk samples exceeded the maximum levels of the European Union, i.e. 0.05 μg/l and Food Safety and Standards Authority of India (FSSAI), i.e. 0.5 μg/l for AFM1 in milk, respectively. On analysis of species variation, buffalo milk (prevalence: 56.6%; mean concentration: 0.42±0.9 μg/l) was found to have higher AFM1 levels than cow milk (prevalence: 56.0%; mean concentration: 0.19±0.3 μg/l), with statistically significant difference between mean concentrations (P<0.01) and non-significant difference between AFM1 prevalence (P=0.91). Furthermore, milk from commercial dairy farms (prevalence: 64.7%; mean concentration: 0.34±0.65 μg/l) was found to be more contaminated than from household dairy establishments (prevalence: 47.8%; mean concentration: 0.19±0.65 μg/l). The risk factors ‘above average milk yield/day’ (odds ratio (OR): 2.4) and ‘poor animal hygiene’ (OR: 1.9) were identified at animal level, and ‘intensive dairy farming’ (OR: 3.1) and ‘animal feed without aflatoxin binder’ (OR: 4.7) as farm level risk factors for AFM1 excretion above maximum levels of European Union in milk. Among cow breeds, the milk from ‘non-descript’ breed (OR: 11.5) was found to be most contaminated with AFM1 and the least from Jersey breed (OR: 1.0). The present study highlighted the presence of AFM1 in milk samples; therefore, regular monitoring of AFM1 in milk is required so that high risk regions and associated risk factors can be addressed appropriately.

Abnormal Freezing of Milk Caused by Ice-Nucleating Bacteria

1989 ◽  
Vol 52 (7) ◽  
pp. 496-497
Author(s):  
R. T. MARSHALL ◽  
S. A. WIJERATNE ◽  
S. D. JESSE
Keyword(s):  
Pseudomonas Fluorescens ◽  
Freezing Point ◽  
Gram Negative ◽  
Milk Samples ◽  
Bulk Milk

Cultures of ice nucleating bacteria isolated from raw producer bulk milk were gram negative rods, mostly Pseudomonas fluorescens. Milk samples from which the cultures were isolated prefroze during supercooling in the cryoscopic test of freezing point. Grown under conditions of restricted nutrition, cells transferred to normally freezing samples imparted the ice nucleating factor.

scholarly journals Somatic Cells in Bulk Samples and Purchase Prices of Cow Milk

2017 ◽  
Vol 65 (3) ◽  
pp. 879-892
Author(s):  
Jindřich Kvapilík ◽  
Oto Hanuš ◽  
Petr Roubal ◽  
Jan Říha ◽  
Petr Urban ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Count ◽  
Protein Content ◽  
Somatic Cell Count ◽  
Freezing Point ◽  
Fat Content ◽  
Regression Coefficients ◽  
Cow Milk ◽  
Milk Samples ◽  
Bulk Milk

There were calculated the somatic cell count (SCC) 209 (36 – 468) 103ml–1, the total count of microorganisms (TCM) 25 103ml–1 (from 5 to 377), fat 3.84 % (from 3.23 to 4.46) and protein content 3.39 % (from 3.04 to 3.75) and milk freezing point (MFP) –0.525 °C (from –0.534 to –0.395) of the 522 monthly bulk milk samples from 11 experimental stables during the period from 2012 to 2015. Residues of inhibitory substances were not detected in any sample. Milk sale reached 7,999 liters (l) with fluctuating between 6,150 and 10,532 l per cow. This can be deduced from the regression coefficients that due to increase in the SCC by 100 103ml–1 the TCM increased by 2.9 to 4.2 103ml–1, the fat content decreased by 0.09 to 0.13 % and protein about 0.01 to 0.05 %. Influence of SCC, TCM and the fat and protein content calculated from monthly samples for individual stables can be estimated at –0.12 CZC, fluctuations between the stables at +0.46 to –0.84 CZC per l of milk. The increase in milk price by 0.17 CZC in the range of –0.92 to +0.92 CZC per l of milk corresponds to averages of indicators calculated from 522 samples.

scholarly journals  Effects of season and time of milking on spontaneous and induced lipolysis in bovine milk fat

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 20-26 ◽  
Author(s):  
R. Toušová ◽  
L. Stádník ◽  
J. Ducháček
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Milk Fat ◽  
Bovine Milk ◽  
Milk Samples ◽  
Bulk Milk ◽  
Bulk Tank

The effects were evaluated of different factors on the level of spontaneous (SPO) and induced (IND) lipolysis as defined by the content of free fatty acids (FFA) in milk. Milk samples were collected at monthly intervals throughout the year from both morning and evening milkings either individually in a milking parlour (SPO; n = 10) or from the bulk tank (IND; n = 10). The data were analysed using SAS 9.1. More intensive SPO was observed from March to May with higher FFA contents (+0.034 to +0.523 mmol/100 g of fat; P &lt; 0.05&ndash;0.01), and also from September to November (+0.077 to +0.292 mmol/100 g of fat; P &lt; 0.05). More intensive SPO was also detected in the evening milk than in that coming from morning milking (+0.062 to +0.556 mmol/100 g of fat; P &lt; 0.05&ndash;0.01). SPO measured immediately after milking was affected by the season and time of milking. The content of FFA characterising IND in bulk milk (0.33&ndash;1.10 mmol/100&nbsp;g of fat) was higher (P &lt; 0.05&ndash;0.001) than that due to SPO in individual samples (0.21&ndash;0.86 mmol/100 g of fat), especially in those from evening milking compared to morning milking (+0.10 to +0.47 vs. +0.12 to +0.22 mmol/100 g of fat; P &lt; 0.05&ndash;0.001).

scholarly journals Detection of antibiotic residues in locally raw milk by using high performance liquid chromatography at different seasons and the effect of heat treatment on their concentration

2018 ◽  
Vol 41 (2) ◽  
pp. 131-136
Author(s):  
Ahmed Salman Moqef Al- kurashi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Bovine Milk ◽  
Raw Milk ◽  
Antibiotic Residues ◽  
Milk Samples ◽  
Bulk Milk ◽  
Antibiotics Residues ◽  
High Degree

     The aim of this study was to determine the level of antibiotics residues in the locally produced cows raw milk as well as to evaluate the effect of different commercial heat treated process on the level of antibiotics residues in milk. A total of 52 locally produced milk samples were collected from apparently healthy cows in AL-Fudhailia village, milk cans (50 Kg), bulk milk tanks (1, 5 and 10 tons) which belonged to the milk reception and collection centers and dairy plants in Baghdad were collected during beginning of December 2015 to the end of April 2016. Results revealed by using high performance liquid chromatography that there was a significant seasonal variation in the residual concentration of each detected antibiotic in milk samples, where it was found that all cow's milk samples had significantly higher concentrations of antibiotic residues in spring than in winter seasons. Generally by excluding both the season and the kind of antibiotic, it was found that milk samples that were collected from 50 Kg milk cans recorded significantly highest antibiotic residues followed by bulk milk tanks of 1 and 5 tons in comparison to 10 tons. Antibiotics recovery experiment was conducted by spiking the bovine milk samples with Known concentrations four ß-lactams (Benzylpencillin, Cloxacillin, Amoxcilin, Ampcilline) and other five detected antibiotics and then exposed to one of the three different commercial heat treatments. The results showed that the pasteurization process (63°C/30 min.) a slight inactivation of four ß-lactams and other five detected antibiotics, whereas 80°C/5 min. high degree of antimicrobial loss at the rate of 89% and 82%. However, boiling at 100°C/5 min, high degree of antimicrobial loss at the rate of 100% and 90% respectively.

scholarly journals AMINO ACID STATUS OF BOVINE MILK UNDER SEASONAL VARIATION AROUND SINGRAULI DISTRICT

2021 ◽  
pp. 1-4
Author(s):  
RAJESH K. PATEL ◽  
P. K. SINGH ◽  
RAJESH PANDEY ◽  
BABBULAL PATEL ◽  
SANDEEP MISHRA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acid Content ◽  
Bovine Milk ◽  
Amino Acid Content ◽  
Buffalo Milk ◽  
Milk Samples ◽  
Acid Status

Objective: The present work deals with the concerned study to determine the influence of variable season on amino acid evaluation from 90 bovine milk collected (March 2018 to February 2019) samples belongs to four tehsils of Singrauli district. Methods: An aliquot of hydrolysate of collected bovine milk samples were injected into the column (Shim-pack ISC-07/S1504 Na) of the higher performance liquid chromatography-based amino acid analyzer. Results: Most abundant amino acids were glutamic acid followed by proline and leucine. Average amino acid content comparison, results, in the rainy season, maximum amino acid content was in Deosar and minimum in Singrauli, In winter amino acid is higher in Waidhan and minimum in Deosar and maximum amino acid content was recorded intended for Waidhan and minimum for Deosar in summer. Concentration of amino acid in cow milk was found to be in order of glycine<histidine<alanine<methionine<isoleucine<phenylalanine<threonine<tyrosine<serine<lysine<valine and aspartic acid<arginine<leucine<proline<glutamic acid. Amino acid status in buffalo milk sample was found in order Glycine<histidine<methionine<alanine<phenylalanine<isoleucine<threonine<arginine<tyrosine<serine and lysine<aspartic acid<valine<leucine<proline<glutamic acid. All the amino acids are evaluated as per the mean (n = 6) mean±SD. Conclusion: It is concluded that cow and buffalo milk samples contained all the amino acids but differed in the contents of certain amino acids. Such variation is purely biological and genetic and is intended for the nourishment of the young. The current investigation would be valuable for the dairy processing industries to formulate nutritionally improved milk-based functional products for a vulnerable segment of the population.

scholarly journals Seasonally Feed-Related Aflatoxins B1 and M1 Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Saideh Mozafari ◽  
Mohammad Mohsenzadeh ◽  
Jalil Mehrzad
Keyword(s):  
Dairy Herd ◽  
Milk Samples ◽  
Bulk Milk ◽  
Four Seasons ◽  
Immune Incompetence ◽  
The Impact ◽  
Cattle Farms

To comparatively determine the levels of aflatoxin (AF) B1 in feedstuffs and of AFM1 in milk from semiarid industrial cattle farms in northeastern Iran during four seasons and to elucidate the effects of mixed AFB1 and AFM1 on bovine granulocytes, 72 feedstuffs (concentrate, silage, and totally mixed ration (TMR)) and 200 bulk milk samples were simultaneously collected for ELISA-based AFs detection. Isolated blood and milk neutrophils (n=8/treatment) were also preincubated with mix of 10 ng/ml AFB1 and 10 ng/ml AFM1 for 12 h; the impact was assessed on neutrophils functions. AFB1 levels in feedstuffs averaged 28 μg/kg (4–127 μg/kg), with TMR maximal (38±6.3 μg/kg), concentrate (32±6.5 μg/kg), and silage (16±1.5 μg/kg). The levels of AFB1 and AFM1 in feedstuffs and milk averaged 42±9.3, 27±2.8, 26±4.1, and 18.5±2.8 μg/kg and 85±7.3, 62±6.1, 46±6.2, and 41±6.5 ppb μg/kg in winter (maximal), autumn, spring, and summer, respectively. Mix of AFB1 and AFM1 weakened various functions of granulocytes. It adds new reason why during winter semiarid raised food-producing animals show more immune-incompetence.

Proteolysis detection in milk: II. The effect of preincubation of raw and laboratory pasteurized bulk milk samples on tyrosine value and its relationship with bacterial populations

1973 ◽  
Vol 40 (3) ◽  
pp. 383-392 ◽  
Author(s):  
H. S. Juffs
Keyword(s):  
Major Contributor ◽  
Milk Supply ◽  
Bacterial Populations ◽  
Milk Samples ◽  
Bulk Milk ◽  
Upper Limit ◽  
Principal Source ◽  
Before And After

SummaryA study is reported of the effect of preincubation of raw and laboratory pasteurized bulk milk samples on the tyrosine value (TV) of milk and its relationship with bacterial populations. For raw samples, mean TVs before and after preincubation for 20±2 h at both 25 and 30 °C differed (P < 0·001). After preincubation at 30 °C, the TV of 47 % of raw samples exceeded that considered to represent the upper limit of variation in the TV of a normal bulk milk supply. At this temperature, however, there was no indication that proteolytic psychrotrophs were a major contributor to increased TV. With preincubation of raw samples at 25 °C, an increase to above the upper limit of variation occurred in only 20 % of samples; however, proteolytic psychrotrophs appeared more active at this temperature. In similar samples preincubated after laboratory pasteurization, large increases occurred in TV after 20 ±2 h at 30 °C, while at 25 °C the increases were much smaller and of the order found for preincubated raw samples. The results showed, however, that nonpsychrotrophic bacteria were also the principal source of proteinase giving rise to increased TVs in laboratory pasteurized samples.

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