Cell Cycle Regulation in the Estrogen Receptor Beta (ESR2)-Overexpressing Hep3B Hepatocellular Carcinoma Cell Line

2015 ◽  
Vol 預刊文章 (預刊文章) ◽  
pp. 1-7 ◽  
Author(s):  
Yi-sheng Liu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Estrogen Receptor ◽  
Cell Cycle Regulation ◽  
Carcinoma Cell ◽  
Estrogen Receptor Beta ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell ◽  
Cycle Regulation ◽  
Receptor Beta

scholarly journals Identification of MicroRNAs Involved in Growth Arrest and Apoptosis in Hydrogen Peroxide-Treated Human Hepatocellular Carcinoma Cell Line HepG2

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Yuan Luo ◽  
Xinyu Wen ◽  
Ling Wang ◽  
Jing Gao ◽  
Zi Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Target Genes ◽  
Carcinoma Cell ◽  
Growth Arrest ◽  
Putative Target ◽  
Hepatocellular Carcinoma Cell Line ◽  
Human Hepatocellular Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell ◽  
Carcinoma Cell Line Hepg2

Although both oxidative stress and microRNAs (miRNAs) play vital roles in physiological and pathological processes, little is known about the interactions between them. In this study, we first described the regulation of H2O2in cell viability, proliferation, cycle, and apoptosis of human hepatocellular carcinoma cell line HepG2. Then, miRNAs expression was profiled after H2O2treatment. The results showed that high concentration of H2O2(600 μM) could decrease cell viability, inhibit cell proliferation, induce cell cycle arrest, and finally promote cell apoptosis. Conversely, no significant effects could be found under treatment with low concentration (30 μM). miRNAs array analysis identified 131 differentially expressed miRNAs (125 were upregulated and 6 were downregulated) and predicted 13504 putative target genes of the deregulated miRNAs. Gene ontology (GO) analysis revealed that the putative target genes were associated with H2O2-induced cell growth arrest and apoptosis. The subsequent bioinformatics analysis indicated that H2O2-response pathways, including MAPK signaling pathway, apoptosis, and pathways in cancer and cell cycle, were significantly affected. Overall, these results provided comprehensive information on the biological function of H2O2treatment in HepG2 cells. The identification of miRNAs and their putative targets may offer new diagnostic and therapeutic strategies for liver cancer.

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