The Effect of Cell Cycle and Expression of Cyclin B1 and Cyclin C Protein in Hepatocellular Carcinoma Cell Line HepG2 and SMMC-7721 after of Silencing β-Catenin Gene

2011 ◽  
Vol 59 (114) ◽  
Author(s):  
Bin Wang ◽  
Xunsun ◽  
Jin-Yu Liu ◽  
Dan Yang ◽  
Luo-Luo Yang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Cyclin B1 ◽  
C Protein ◽  
Cyclin C ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell ◽  
Carcinoma Cell Line Hepg2

Cytotoxic Effects of 2-Methoxyestradiol in the Hepatocellular Carcinoma Cell Line HepG2

Pharmacology ◽  
2009 ◽  
Vol 84 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Reem N. Abou El Naga ◽  
Ebtehal El-Demerdash ◽  
Samar S. Youssef ◽  
Ashraf B. Abdel-Naim ◽  
Mahmoud El-Merzabani
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Cytotoxic Effects ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell ◽  
Carcinoma Cell Line Hepg2

scholarly journals Isolation of cancer stem-like cells from hepatocellular carcinoma cell line HepG2 by methods of magnetic-activated cell sorting, spheroid culture, and anti-tumor drug-resistant selection: A primary evaluation

2020 ◽  
Vol 7 (1) ◽  
pp. 279-289
Author(s):  
Sinh Truong Nguyen ◽  
Luong Sy Nguyen ◽  
Thao Hoang Phuong Nguyen ◽  
Phuc Hong Vo ◽  
Nghia Minh Do ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Cell Sorting ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Spheroid Culture ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell ◽  
Carcinoma Cell Line Hepg2 ◽  
Magnetic Activated Cell Sorting

Introduction: Recently reported data have suggested that only a small subset of cancer cells possess the capability to initiate malignancies. These observations were based on investigation of cells within the primary tumors displaying a distinct surface marker pattern. CD133 marker is a putative hematopoietic and neuronal stem cell marker, which is also considered to be a tumorigenic marker in brain, prostate and liver. Recent studies have shown that a small population of CD133-positive cells, indeed, exists in human hepatocellular carcinoma (HCC) cell lines and primary HCC tissues. This study was aimed at isolating the cancer stem-like cells from hepatocellular carcinoma cell line HepG2 using three different methods: magnetic-activated cell sorting (MACS), spheroid culture (SC), and anti-tumor drug (ATD) resistant selection. Methods: HepG2 hepatocellular carcinoma cells were expanded to yield enough cells that could be used to isolate cancer stem-like cells by these three methods. For MACS, cancer stem-like cells were sorted using anti-CD133 monoclonal antibody. For the second method, cancer stem-like cells were enriched by selection of anti-tumor drug resistance property. Lastly, for the third method, three-dimensional (3D) culture was used to enrich for the cancer stem-like cells. The cells obtained by the three methods were expanded to obtain an adequate number of cells for confirmation of CD133 expression. Results: The expression of CD133+ cells in the three methods was found to be different. In the MACS method, the expanded CD133+ sorted cells cultured through 2 passages only contained 0.40 % CD133+ cells. In the 3D spheroid cell culture, of the population of cells there were 38.39 % that were CD133+ cells. Lastly, in the anti-tumor drug (doxorubicin at 150 nM) resistant selection, 66.22 % were CD133+ cells. Conclusion: This study shows that isolation of HepG2 derived CD133+ population by culture with doxorubicin (150 nM) yields the highest efficiency and purity of the 3 methods studied.

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