scholarly journals Immobilization of Erwinia sp. D12 Cells in Alginate-Gelatin Matrix and Conversion of Sucrose into Isomaltulose Using Response Surface Methodology

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Haroldo Yukio Kawaguti ◽  
Priscila Hoffmann Carvalho ◽  
Joelise Alencar Figueira ◽  
Hélia Harumi Sato
Keyword(s):  
Sodium Alginate ◽  
Food Industry ◽  
Cell Mass ◽  
Batch Process ◽  
Structural Isomer ◽  
Gelatin Matrix ◽  
Alginate Concentration ◽  
Cell Mass Concentration ◽  
Independent Parameters ◽  
Sucrose Isomerase

Isomaltulose is a noncariogenic reducing disaccharide and also a structural isomer of sucrose and is used by the food industry as a sucrose replacement. It is obtained through enzymatic conversion of microbial sucrose isomerase. An Erwinia sp. D12 strain is capable of converting sucrose into isomaltulose. The experimental design technique was used to study the influence of immobilization parameters on converting sucrose into isomaltulose in a batch process using shaken Erlenmeyer flasks. We assessed the effect of gelatin and transglutaminase addition on increasing the reticulation of granules of Erwinia sp. D12 cells immobilized in alginate. Independent parameters, sodium alginate concentration, cell mass concentration, CaCl2 concentration, gelatin concentration, and transglutaminase concentration had all a significant effect (P<0.05) on isomaltulose production. Erwinia sp. D12 cells immobilized in 3.0% (w/v) sodium alginate, 47.0% (w/v) cell mass, 0.3 molL-1 CaCl2, 1.7% (w/v) gelatin and 0.15% (w/v) transglutaminase presented sucrose conversion into isomaltulose, of around 50–60% in seven consecutive batches.

scholarly journals Immobilization of permeabilized cells of baker’s yeast for decomposition of H2O2 by catalase

2019 ◽  
Vol 21 (2) ◽  
pp. 59-63
Author(s):  
Ilona Trawczyńska
Keyword(s):  
Hydrogen Peroxide ◽  
Catalase Activity ◽  
Cell Mass ◽  
Alginate Beads ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast ◽  
Permeabilized Cells ◽  
Alginate Concentration ◽  
Cell Mass Concentration

Abstract Permeabilization is one of the effective tools, used to increase the accessibility of intracellular enzymes. Immobilization is one of the best approaches to reuse the enzyme. Present investigation use both techniques to obtain a biocatalyst with high catalase activity. At the beginning the isopropyl alcohol was used to permeabilize cells of baker’s yeast in order to maximize the catalase activity within the treated cells. Afterwards the permeabilized cells were immobilized in calcium alginate beads and this biocatalyst was used for the degradation of hydrogen peroxide to oxygen and water. The optimal sodium alginate concentration and cell mass concentration for immobilization process were determined. The temperature and pH for maximum decomposition of hydrogen peroxide were assigned and are 20°C and 7 respectively. Prepared biocatalyst allowed 3.35-times faster decomposition as compared to alginate beads with non permeabilized cells. The immobilized biocatalyst lost ca. 30% activity after ten cycles of repeated use in batch operations. Each cycles duration was 10 minutes. Permeabilization and subsequent immobilization of the yeast cells allowed them to be transformed into biocatalysts with an enhanced catalase activity, which can be successfully used to decompose hydrogen peroxide.

scholarly journals Optimization of alcoholic fermentation using immobilized yeast cells in calcium alginate gel

2015 ◽  
pp. 207-218
Author(s):  
Jovana Djuran ◽  
Zorana Roncevic ◽  
Bojana Bajic ◽  
Sinisa Dodic ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Yeast Extract ◽  
Fossil Fuels ◽  
Alcoholic Fermentation ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Yeast Cells ◽  
Glucose Content ◽  
Alginate Concentration ◽  
Economic Analyses

Ethanol is an important industrial chemical with emerging potential as a biofuel to replace fossil fuels. In order to enhance the efficiency and yield of alcoholic fermentation, combined techniques such as cells immobilization and media optimization have been used. The aim of this study was the optimization of sodium alginate concentration and glucose and yeast extract content in the media for ethanol production with immobilized cells of Saccharomyces cerevisiae. Optimization of these parameters was attempted by using a Box-Behnken design using the response surface methodology. The obtained model predicts that the maximum ethanol content of 7.21% (v/v) is produced when the optimal values of sodium alginate concentration and initial content of glucose and yeast extract in the medium are 22.84 g/L, 196.42 g/L and 3.77 g/L, respectively. To minimize the number of yeast cells "eluted" from the alginate beads and residual glucose content in fermented media, additional two sets of optimization were made. The obtained results can be used for further techno-economic analyses of the process to select the optimum conditions of the fermentation process for industrial application.

scholarly journals Anti-Streptococcus mutans and anti-biofilm activities of dextranase and its encapsulation in alginate beads for application in toothpaste

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10165
Author(s):  
Nucharee Juntarachot ◽  
Sasithorn Sirilun ◽  
Duangporn Kantachote ◽  
Phakkharawat Sittiprapaporn ◽  
Piyachat Tongpong ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Calcium Chloride ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Biofilm Formation ◽  
Alginate Beads ◽  
Oral Diseases ◽  
Sensory Test ◽  
Alginate Concentration ◽  
The Stability

Background The accumulation of plaque causes oral diseases. Dental plaque is formed on teeth surfaces by oral bacterial pathogens, particularly Streptococcus mutans, in the oral cavity. Dextranase is one of the enzymes involved in antiplaque accumulation as it can prevent dental caries by the degradation of dextran, which is a component of plaque biofilm. This led to the idea of creating toothpaste containing dextranase for preventing oral diseases. However, the dextranase enzyme must be stable in the product; therefore, encapsulation is an attractive way to increase the stability of this enzyme. Methods The activity of food-grade fungal dextranase was measured on the basis of increasing ratio of reducing sugar concentration, determined by the reaction with 3, 5-dinitrosalicylic acid reagent. The efficiency of the dextranase enzyme was investigated based on its minimal inhibitory concentration (MIC) against biofilm formation by S. mutans ATCC 25175. Box-Behnken design (BBD) was used to study the three factors affecting encapsulation: pH, calcium chloride concentration, and sodium alginate concentration. Encapsulation efficiency (% EE) and the activity of dextranase enzyme trapped in alginate beads were determined. Then, the encapsulated dextranase in alginate beads was added to toothpaste base, and the stability of the enzyme was examined. Finally, sensory test and safety evaluation of toothpaste containing encapsulated dextranase were done. Results The highest activity of the dextranase enzyme was 4401.71 unit/g at a pH of 6 and 37 °C. The dextranase at its MIC (4.5 unit/g) showed strong inhibition against the growth of S. mutans. This enzyme at 1/2 MIC also showed a remarkable decrease in biofilm formation by S. mutans. The most effective condition of dextranase encapsulation was at a pH of 7, 20% w/v calcium chloride and 0.85% w/v sodium alginate. Toothpaste containing encapsulated dextranase alginate beads produced under suitable condition was stable after 3 months of storage, while the sensory test of the product was accepted at level 3 (like slightly), and it was safe. Conclusion This research achieved an alternative health product for oral care by formulating toothpaste with dextranase encapsulated in effective alginate beads to act against cariogenic bacteria, like S. mutants, by preventing dental plaque.

Effect of Sodium Alginate Concentration During Laser-Assisted Printing of Alginate Tubes

2012 ◽  
Author(s):  
Jingyuan Yan ◽  
Hemanth Gudapati ◽  
Yong Huang ◽  
Changxue Xu
Keyword(s):  
Sodium Alginate ◽  
Three Dimensional ◽  
Free Form ◽  
Thin Wall ◽  
Imaging Analysis ◽  
Tissue Constructs ◽  
Alginate Concentration ◽  
Printing Technique ◽  
Resolution Imaging ◽  
Tube Structure

For the free-form fabrication of various tissue constructs, three-dimensional (3D) additive printing technology has emerged as a promising approach for organ fabrication. This study aims to print a tube structure using a laser-assisted orifice-free printing technique and further investigate the effect of sodium alginate concentration on the tube wall thickness. Alginate tubes have been successfully printed. It is found that highly viscous materials can be laser printed into well-defined tube structures. A higher concentration solution such as the 8% sodium alginate solution leads to a thin wall, meaning a better resolution. Imaging analysis also illustrates that higher concentration solutions help develop smooth, slim jets upon the incidence of laser pulse.

scholarly journals The Effect Of Sodium Alginate Concentration On The Rheological Parameters Of Spinning Solutions

2015 ◽  
Vol 15 (2) ◽  
pp. 123-126
Author(s):  
Magdalena Brzezińska ◽  
Grzegorz Szparaga
Keyword(s):  
Rheological Properties ◽  
Sodium Alginate ◽  
Calcium Alginate ◽  
Solution Method ◽  
Polymer Solutions ◽  
Rheological Parameters ◽  
Wet Spinning ◽  
Alginate Concentration ◽  
Preliminary Research ◽  
Alginate Fibres

Abstract The aim of the study was to determine the rheological properties of solutions of two types of sodium alginate in water. Rheological studies were carried out to determine the rheological properties of the spinning solutions. Polymer solutions of different concentrations were obtained. Based on the preliminary research of the concentrations of solutions, the proper n and k parameters were selected in order to obtain fibre by wet spinning from solution method. For selected concentrations of polymer solutions, the calcium alginate fibres were obtained.

scholarly journals Optimization of Water Fraction Gel Formula of Binahong Leaf (Anredera cordifolia (Ten.) Steen) With Gelling Agent of Sodium Alginate and Carboxymethyl Chitosan Combination

2018 ◽  
Vol 23 (3) ◽  
pp. 97
Author(s):  
Citra Ariani Edityaningrum ◽  
Kintoko Kintoko ◽  
Feby Zulien ◽  
Lina Widiyastuti
Keyword(s):  
Sodium Alginate ◽  
Carboxymethyl Chitosan ◽  
T Test ◽  
Gelling Agent ◽  
Physical Parameters ◽  
Water Fraction ◽  
Lattice Design ◽  
Alginate Concentration ◽  
Significant Difference ◽  
Optimum Formula

Water fraction of binahong (Anredera cordifolia (Ten.) Steen)  leaf has been proven to heal ulcus diabetic. In order to make the use easier and more practical, in this study the water fraction of binahong leaves is formulated in gel preparation form. This study was conducted to obtain a comparison of the amount of sodium alginate and carboxymethyl chitosan as gelling agent to produce gel that meets the good physical requirements. The method used to determine the amount of comparison of sodium alginate and carboxymethyl chitosan is to use Simplex Lattice Design (SLD). Eight variations of gel formula are designed with the ratio of sodium alginate:  carboxymethyl chitosan as follows: F1 (0:3% w/w), F2 (0:3% w/w), F3 (0.75:2.25% w/w), F4 (1.5:1.5 % w/w), F5 (1.5:1.5% w/w), F6 (2.25:0.75% w/w), F7 (3:0% w/w), F8 (3:0% w/w). Physical parameters observed included pH, viscosity, dispersion, and adherence. Data obtained compared with the actual conducted by researchers using one sample test t-test with 95% confidence level. Based on the results of the study, increased sodium alginate concentration can increase viscosity and adhesiveness and decrease the pH and spreadability of the gel. One sample t-test analysis shows that there is no significant difference between predicted parameter value and actual result so SLD equation can be used to construct gel formula of water fraction of binahong leaf. The conclusion is a combination of sodium alginate 1.546% w/w and carboxymethyl chitosan 1.454% w/w yield gel optimum. Physical parameter response of optimum formula is pH 5.86; viscosity 2000 cps; spreadability of 21,96 cm2; and adhesiveness of 19,81 seconds.

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