scholarly journals Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. Using Mature Zygotic Embryos Explants

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Hussien H. Daffalla ◽  
Eltayb Abdellatef ◽  
Elsadig A. Elhadi ◽  
Mutasim M. Khalafalla
Keyword(s):  
Growth Regulators ◽  
Adventitious Shoots ◽  
Normal Growth ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Boscia Senegalensis

The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0 mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0 mg/L BA. 67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25 mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2 cm) was produced on 0.25 mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed.

scholarly journals Callus Induction and Shoot Formation for Mexican Red Bean (Phaseolus vulgaris L.) Pinto Cultivar in Vitro

2020 ◽  
pp. 1887-1893
Author(s):  
Rasha K. Mohammed Al-Saedi ◽  
Ansam G. Abdulhalem
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Phaseolus Vulgaris L ◽  
Different Types ◽  
Red Bean ◽  
Internode Explants

     The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as  internodes, cotyledons and roots,  were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.

scholarly journals Regeneração in vitro de Passiflora miniata Mast

2017 ◽  
Vol 23 (1) ◽  
pp. 88 ◽  
Author(s):  
Paula Pinheiro Carvalho ◽  
Camila Aparecida Antoniazzi ◽  
Nayara Tayane Silva ◽  
Andréia Izabel Mikovski ◽  
Maurecilne Lemes Silva ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Wild Species ◽  
De Novo ◽  
Direct Organogenesis ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Indirect Organogenesis ◽  
Red Coloration ◽  
Number Of Shoots

Passiflora miniata is a wild species native to the Southern Amazon, with ornamental potential due to the beauty of its flowers of intense red coloration. Reports in the literature about the species are still insipid. The aim of the present study was to induce the regeneration of P. miniata by the de novo organogenesis from mature zygotic embryos. The zygotic embryos were isolated and cultivated into the MS medium with the addition of 6-Benzyladenine (BA), Thidiazuron (TDZ) and Kinetin (KIN) growth regulators. The de novo regeneration from the zygotic embryos occurred directly and indirectly. A percentage of 80% of the explants cultivated in the presence of BA had direct organogenesis and 20% by the indirect way, with TDZ 60% were regenerated by the direct and 40% by the indirect way. Regarding the treatments with KIN, 58% of the explants had regeneration by direct and 42% by the indirect organogenesis. The development of shoot primordia initiated with the formation of organogenic structures that later differentiated into multi-shoots. The highest mean number of shoots (40.0 shoots per explants) was obtained on 0.75 mg L-1BA. Conversely, using 0.50 mg L-1 TDZ or KIN, the highest number of shoots were 7.2 and 3.6, respectively.

scholarly journals Neoformação in vitro de Parte Aérea e Enraizamento Adventício de Brotações de Tabernaemontana catharinensis A. DC

2020 ◽  
Vol 24 (1) ◽  
pp. 36-40
Author(s):  
Marcelo Vielmo Afonso ◽  
Juçara Terezinha Paranhos ◽  
Luciane Almeri Tabaldi ◽  
Aline Soares Pereira ◽  
Athos Odin Severo Dorneles
Keyword(s):  
Culture Medium ◽  
Native Plants ◽  
Adventitious Shoots ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Root Number ◽  
Shoot Weight ◽  
Stimulation Of

Plantas nativas com potencial medicinal têm acentuada valorização mundial, contudo, ainda muitas espécies requerem estudos ecológicos e fisiológicos, que auxiliarão na multiplicação e conservação das espécies. Assim se objetivou, neste trabalho, avaliar o efeito da 6-benzilaminopurina (BAP) associada ao ácido naftalenoacético (ANA) no estímulo de formação de brotações aéreas a partir de segmentos cotiledonares de Tabernaemontana catharinensis e posterior indução do enraizamento em cultivo suplementado com ácido indolbutírico (AIB). Segmentos cotiledonares de plântulas obtidasin vitro foram inoculados em meio de cultivo (MS), contendo diferentes combinações de BAP e ANA: 0,0 x 0,0; 1,0 x 0,1; 2,0 x 0,2; 4,0 x 0,4; 6,0 x 0,6 mg L-1, respectivamente. Após a obtenção de brotações aéreas, as mesmas foram cultivadas em meio contendo AIB, por 30 dias, nas concentrações de 0,0; 1,0; 2,0; 4,0 ou 6,0 mg L-1. A organogênese direta de brotações adventícias foi induzida sem a necessidade de fitorreguladores de crescimento (BAP x ANA) no meio de cultivo. No entanto, houve um incremento no número de brotos e folhas, comprimento e massa fresca das brotações na presença dos fitorreguladores. A rizogênese em brotações aéreas, quando cultivadas em meio enriquecido com 6,0 mg L-1de AIB atingiu 80%. As variáveis: comprimento, massa fresca e número de raízes foram potencializadas com adição de AIB. Portanto, os fitorreguladores de crescimento promovem a obtenção de um maior número de microplantas completas desta espécie, a partir de segmentos cotiledonares cultivados in vitro. Palavras-chave: Planta Medicinal. Auxinas. Citocininas. Parâmetros Fisiológicos.   Abstract Native plants with medicinal potential have outstanding worldwide appreciation, however still many species require ecological and physiological studies, which will assist in the species multiplication and conservation. Thus, the aim of this study was to evaluate the effect of naphthalene acetic acid (NAA) associated with 6-benzylaminopurine (BAP) on the stimulation of shoot formation from cotyledonary segments of Tabernaemontana catharinensis and subsequent induction of rooting in Indolbutyric-acid supplementation (IBA). Cotyledonous segments of seedlings obtained in vitro were inoculated in culture medium (MS) containing different BAP and NAA combinations: 0.0 x 0.00 ; 1.0 x 0.1; 2.0 x 0.2; 4.0 x 0.4 ; 6.0 x0.6 mg L-1, respectively. After obtaining aerial shoots, they were cultivated in medium containing IBA, for 30 days, at concentrations of 0.0; 1.0; 2.0; 4.0 or 6.0 mg L-1. Direct organogenesis of adventitious shoots can be induced without the need for growth phytoregulators (BAP x NAA) in the culture medium. However, there is an increase in leaf number, shoot length and fresh shoot weight in the presence of phytoregulators. The rhizogenesis in shoots when grown in medium supplemented with 6.0 mg L-1 of IBA reaches 80%. The variables length, fresh mass, and root number were potentiated with the IBA addition. Therefore, growth regulators make it possible to obtain a larger number of complete microplants of this species from in vitro cultivated cotyledonary segments.  Keywords: Medicinal Plant. Auxins. Cytokinins. Physiological Parameters.  

scholarly journals In Vitro Clonal Propagation of Vanilla (Vanilla planifolia ‘Andrews’)

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 454-458 ◽  
Author(s):  
Hilda E. Lee-Espinosa ◽  
Joaquín Murguía-González ◽  
Benjamín García-Rosas ◽  
Ana L. Córdova-Contreras ◽  
Antonio Laguna-Cerda ◽  
...  
Keyword(s):  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Vanilla Planifolia ◽  
Number Of Shoots

A complete and efficient regeneration protocol was developed for Vanilla planifolia ‘Andrews’, an endangered orchid species that represents an important crop in several tropical countries. Axillary buds excised from the first to the eighth node, considering the first to fourth nodes as “young” (zone 1) and the fifth to eighth as “mature” (zone 2), were cultured on Murashige and Skoog (MS) medium supplemented with 5.73, 7.64, 9.55, or 11.46 μm 6-benzylaminopurine (BA) for shoot induction and in combination with 4.45 μm naphthalene acetic acid (NAA) to induce multiple shoot proliferation. Cytokinin concentration and bud position in the stem had a significant effect on the number of shoots regenerated. The greatest shoot formation per explant, for the two tested zones, was obtained with 9.55 μm BA on MS medium supplemented with 100 mg·L−1 myoinositol, 150 mg·L−1 citric acid, 100 mg·L−1 ascorbic acid, and 20 g·L−1 sucrose. Young buds from zone 1 were able to form an average of 18.5 ± 2.4 shoots per explant, whereas buds from zone 2 induced a maximum of 11.0 ± 1.0 shoots per explant. Plants of 2 to 3 cm height developed a root system in half-strength MS medium supplemented with 0.44 μm NAA and, once they reached 5 cm height on average, were transferred to greenhouse conditions for their acclimatization where a 100% rate survival was achieved. The optimal use of both young and mature buds from each mother plant to induce adventitious shoots permitted a marked increase in the number of shoots per explant. By using all buds from the upper stem part (zone 1 + zone 2) and subculturing every 90 d, the multiplication rate was 1.1 to 1.86 × 105 shoots per bud per year.

scholarly journals In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 416-421 ◽  
Author(s):  
Ze Li ◽  
Xiaofeng Tan ◽  
Zhiming Liu ◽  
Qing Lin ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Adventitious Shoots ◽  
Shoot Multiplication ◽  
Cotyledonary Node ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Camellia Oleifera ◽  
Cotyledonary Nodes

Camellia oleifera Abel. is one of four major woody oil plants in the world. The objective of the current study was to evaluate the effect of different plant growth regulators (PGRs) and concentrations on direct organogenesis using cotyledonary nodes, hypocotyls, and radicle explants. High induction frequency of adventitious shoots were obtained from cotyledonary nodes, hypocotyls, and radicle explants (85.2%, 73.6%, and 41.0%, respectively) when cultured on half-strength Murashige and Skoog (1/2 MS) medium containing 2.0 mg·L−1 6-benzylaminopurine (BA) and 0.1 mg·L−1 indole-3-acetic acid (IAA). Microshoots from cotyledonary nodes, hypocotyls, and radicle explants were then transferred to 1/2 MS medium containing 2.0 mg·L−1 BA and 0.05 mg·L−1 indole-3-butyric acid (IBA) for shoot multiplication, resulting in 6.9 shoots per explant. The shoots were transferred to Woody Plant Medium (WPM) supplemented with various α-naphthalene acetic acid (NAA) and gibberellic acid (GA3) for shoot elongation. The mean length of shoots and the number of leaves per shoot were 3.7 and 6.6 cm, respectively, in WPM supplemented with 0.5 mg·L−1 NAA and 3.0 mg·L−1 GA3. The highest rooting of shoots (90.2%) or the number of roots per shoot (7.2) was obtained when elongated microshoots were transferred to 1/2 MS medium supplemented with 3.5% perlite, 1.0 mg·L−1 IBA and 2.0 mg·L−1 NAA. The rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90.0%. The in vitro plant regeneration procedure described in this study is beneficial for mass propagation and improvement of C. oleifera through genetic engineering.

scholarly journals Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

2013 ◽  
Vol 21 (2) ◽  
pp. 23-30 ◽  
Author(s):  
Fardin Nasri ◽  
Seyed Najmedin Mortazavi ◽  
Nasser Ghaderi ◽  
Taimoor Javadi
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Shoots ◽  
Shoot Length ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Leaf Base

ABSTRACT In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length of shoots and roots were recorded. Adventitious shoots formed directly on the leaf bases without callus intervention. Their number was affected by BAP concentrations. The highest shoots number, six per explants, was obtained at 1 mg·1-1 BAP and 0.1 mg·1-1 IBA. The shoot length decreased with the increasing concentration of BAP. The highest root number (2.7) was formed on shoots cultured on the MS medium containing 0.5 mg·1-1 α- naphthalene acetic acid, and the highest rhizome number (2.2) was formed on the medium with 0.5 mg·1-1 BAP. In vitro rooted plantlets were able to survive and acclimatize in the greenhouse.

scholarly journals In vitro studies on Dendrobium fimbriatum Hk.F – An endangered orchid

2019 ◽  
pp. 100-103
Author(s):  
T. Ramesh ◽  
P. Renganathan ◽  
M. Prabhakaran
Keyword(s):  
Culture Media ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Average Height ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Dendrobium Fimbriatum

A protocol for in vitro propagation from protocorms of Dendrobium fimbriatum a distributed in all over world and highly appreciated as an ornamental, was developed. Two different explants, entire protocorms and longitudinal halves of protocorms, were used. In addition, the effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), supplemented with N6- benzyladenine (BA) (0, 0.5, 1, 2, 3, and 5 mg_L–1) and/or a-naphthalene acetic acid (NAA) at 0, 0.1, and 0.5 mg_L–1 was investigated. Adventitious shoot formation by direct organogenesis was obtained in all treatments; in some cases, the formation of protocorm like bodies was induced. Shoot formation was greater for entire protocorms; the best treatment was MS medium containing at BA 1 to 2 mg_L–1 in combination with at NAA 0.1 mg_L–1. The average height of shoots was three times greater in MS medium than in KC m medium. Sub culturing individual shoots in MS medium without plant growth regulators, but with 1 g_L–1 activated charcoal, allowed further development and rooting. An ex vitro survival rate of almost 100% was achieved. This study represents a comprehensive application for propagation, conservation, and sustainable use of this valuable natural resource.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals In-vitro development of Nauclea diderrichii (de Willd. & Th. Dur) Merrin liquid-M Smedia supplemented with benzyl amino purine (BAP) and naphthalene acetic acid (NAA)

2021 ◽  
Vol 24 (12) ◽  
pp. 2065-2069
Author(s):  
J.O. Afolabi ◽  
R.I. Oyediran ◽  
Y.O. Aguda ◽  
E.A. Adekunle
Keyword(s):  
Growth Regulators ◽  
Adventitious Shoots ◽  
Transition Process ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Temporary Immersion Bioreactor ◽  
Group A ◽  
Bioreactor System ◽  
Vitro Development

The growth of plantlets in Temporary Immersion Bioreactor system (TIBs) relies on initial successful liquid phase transition process. The response of N. diderrichii explants was assessed in liquid-M Smedia with a view to mass produce its seedlings using TIBs. Seven treatments consisting (A) 0.0/0.0, (B) 0.0/0.1, (C) 0.1/0.0, (D) 0.2/0.1, (E) 0.3/0.2, (F) 0.4/0.3 and (G) 0.5/0.4mg/lBAP/NAA combinations were studied. Each group consist of seven replicates and group A without Growth Regulators (GR) serves as control. The results at 4 Weeks after Inoculation (WAI) showed that effects of the growth regulators were significant on shoot length and number of adventitious shoots while number of roots and leaves were closely related. Treatment E produced highest number of adventitious shoots (3.6) which was higher than 0.9 shoots from treatment G and closely related to others. Maximum number of leaves (16.6) was produced by treatment F followed by E (15.7) while the least (12) was obtained in treatment A. The highest number of roots (4.9) was obtained from treatments B, followed by E (4.3) with the lowest being recorded in C (2.43). Liquid MS medium supplemented with 0.3/0.2mg/lBAP/NAA shows some promise for plantlets generation for the purpose of multiple shoot production of N. diderrichii in TIBs.

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