scholarly journals Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Yuzhong Zhang ◽  
Zhen Wang ◽  
Yuehong Wang ◽  
Lei Huang ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Dna Biosensor ◽  
Mercaptoacetic Acid ◽  
Electrochemical Impedance Spectra ◽  
Target Dna ◽  
Covalently Linked ◽  
Film Assembly

A sensitive electrochemical DNA biosensor was prepared based on mercaptoacetic acid (MAA)/gold nanoparticles (AuNPs) modified electrode. Probe DNA (NH2-DNA) was covalently linked to the carboxyl group of MAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxyl-succinimide (NHS). Scanning electron microscopy (SEM) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. The DNA hybridization events were monitored by differential pulse voltammetry (DPV), and adriamycin was used as the electrochemical indicator. Also the factors influencing the performance of the DNA hybridization were investigated in detail. Under the optimal conditions, the signal was linearly changed with target DNA concentration increased from 5.0 × 10−13to 1.0 × 10−9 M and had a detection limit of 1.7 × 10−13 M (signal/noise ratio of 3). In addition, the DNA biosensor showed good reproducibility and stability during DNA assay.

scholarly journals Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3684 ◽  
Author(s):  
Yesong Gu ◽  
Po-Yuan Tseng ◽  
Xiang Bi ◽  
Jason Yang
Keyword(s):  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Complementary Sequence ◽  
Signal Decay ◽  
Target Dna ◽  
Hybridization Efficiency ◽  
Sh Group ◽  
Polymerase Chain ◽  
Or Gene

The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming. In this study, we have proposed a facile strategy to construct a DNA biosensor, in which the platinum electrode was modified with a dual-film of electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) resulting in immobilized gold nanoparticles, with the gold nanoparticles easily immobilized in a uniform distribution. The DNA probe labeled with a SH group was then assembled to the fabricated electrode and employed to capture the target DNA based on the complementary sequence. The hybridization efficiency was evaluated with differential pulse voltammetry (DPV) in the presence of daunorubicin hydrochloride. Our results demonstrated that the peak current in DPV exhibited a linear correlation the concentration of target DNA that was complementary to the probe DNA. Moreover, the electrode could be reused by heating denaturation and re-hybridization, which only brought slight signal decay. In addition, the addition of the oxidized form of nicotinamide adenine dinucleotide (NAD+) could dramatically enhance the sensitivity by more than 5.45-fold, and the limit-of-detection reached about 100 pM.

scholarly journals Application of Gold Nanoparticles for Electrochemical DNA Biosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ahmed Mishaal Mohammed ◽  
Ruslinda A. Rahim ◽  
Ibraheem Jaleel Ibraheem ◽  
Foo Kai Loong ◽  
Hasrul Hisham ◽  
...  
Keyword(s):  
Thin Films ◽  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Base Line ◽  
Frequency Range ◽  
Dna Immobilization ◽  
Electrochemical Dna Biosensor ◽  
Chemically Modified

An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2thin films. The SiO2thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2thin films using APTES and GNPs were successfully developed for DNA detection.

scholarly journals An electrochemical DNA biosensor for trace amounts of mercury ion quantification

2016 ◽  
Vol 14 (5) ◽  
pp. 808-815 ◽  
Author(s):  
Ferdaous Maâtouk ◽  
Mouna Maâtouk ◽  
Karima Bekir ◽  
Houcine Barhoumi ◽  
Abderrazak Maaref ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Mercury Ion ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Dna Oligonucleotides ◽  
Wastewater Samples ◽  
Eis Measurements

In this work we report the development of an electrochemical DNA biosensor with high sensitivity for mercury ion detection. A new matrix based on gold nanoparticles (AuNPs)-glutathione (GSH)/cysteine was investigated. The interaction between DNA oligonucleotides and Hg2+ ions followed by the formation of Thymine–Hg2+–Thymine (T–Hg2+–T) structures was quantified using different electrochemical methods. It has been shown that the electrochemical impedance spectroscopy (EIS) measurements and the differential pulse voltammetry (DPV) confirmed the specific interaction between the oligonucleotide receptor layer and the Hg2+ ions. Besides, the developed sensor exhibited high sensitivity towards mercury among some examined metal ions such as Pb2+, Cu2+ and Cd2+. As a result, a high electrochemical response and low detection limit of 50 pM were estimated in the case of Hg2+ ions. The developed DNA biosensor was applied successfully to the determination of Hg2+ions in wastewater samples.

scholarly journals Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Samsulida Abdul Rahman ◽  
Rafidah Saadun ◽  
Nur Ellina Azmi ◽  
Nurhayati Ariffin ◽  
Jaafar Abdullah ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Peptide Nucleic Acid ◽  
Virus Detection ◽  
Label Free ◽  
Color Changes ◽  
Phosphate Backbone ◽  
Single Base Mismatch ◽  
Target Dna ◽  
Base Mismatch

A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA.

scholarly journals Sensitive and Selective Electrochemical Detection of Epirubicin as Anticancer Drug Based on Nickel Ferrite Decorated with Gold Nanoparticles

Micromachines ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1334
Author(s):  
Mohammad Mehmandoust ◽  
Nevin Erk ◽  
Ceren Karaman ◽  
Fatemeh Karimi ◽  
Sadegh Salmanpour
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Nickel Ferrite ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
X Ray ◽  
Transmission Electron ◽  
The Stability ◽  
Electrochemical Electrode

The accurate and precise monitoring of epirubicin (EPR), one of the most widely used anticancer drugs, is significant for human and environmental health. In this context, we developed a highly sensitive electrochemical electrode for EPR detection based on nickel ferrite decorated with gold nanoparticles (Au@NiFe2O4) on the screen-printed electrode (SPE). Various spectral characteristic methods such as Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-Vis), energy-dispersive X-ray spectroscopy (EDX) and electrochemical impedance spectroscopy (EIS) were used to investigate the surface morphology and structure of the synthesized Au@NiFe2O4 nanocomposite. The novel decorated electrode exhibited a high electrocatalytic activity toward the electrooxidation of EPR, and a nanomolar limit of detection (5.3 nM) was estimated using differential pulse voltammetry (DPV) with linear concentration ranges from 0.01 to 0.7 and 0.7 to 3.6 µM. The stability, selectivity, repeatability reproducibility and reusability, with a very low electrode response detection limit, make it very appropriate for determining trace amounts of EPR in pharmaceutical and clinical preparations.

Development of an electrochemical DNA biosensor based on Quercetin as a new electroactive indicator for DNA hybridization detection

2021 ◽  
Author(s):  
Esmaeel Alipour ◽  
Sheida Norouzi ◽  
Shokoufe Moradi
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Dna Hybridization ◽  
Electrode Surface ◽  
Dna Biosensor ◽  
Carbon Electrode ◽  
Electrochemical Dna Biosensor ◽  
Specific Target ◽  
Target Dna ◽  
Hybridization Detection

Electrochemical DNA biosensor is designed for detection of specific target DNA after hybridization with complementary probe DNA immobilized onto glassy carbon electrode surface. Quercetin was successfully used as a new...

scholarly journals Novel Competitive Voltammetric Aptasensor Based on Electrospun Carbon Nanofibers-Gold Nanoparticles Modified Graphite Electrode for Salmonella enterica serovar Detection

2020 ◽  
Vol 11 (2) ◽  
pp. 8702-8715
Keyword(s):  
Gold Nanoparticles ◽  
Carbon Nanofibers ◽  
Electrochemical Detection ◽  
Salmonella Enterica ◽  
Electrochemical Impedance ◽  
Graphite Electrode ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Pencil Graphite Electrode ◽  
Experimental Conditions

Salmonella enterica is considered one of the most common bacterial agent causes of acute gastroenteritis and foodborne illness in humans worldwide. Antibiotic-resistant is considered as a major problem in Salmonella enterica Serovar. This study introduces a new simple and sensitive aptasensor based on chitosan (Chi)-electrospun carbon nanofibers (CNF) /gold nanoparticles (GNPs) decorated pencil graphite electrode (GE) as a novel platform for electrochemical detection of Salmonella enterica Serovar. A Salmonella-specific recognition aptamer ssDNA sequence was used in the development of this voltammetric biosensor. Electrochemical behaviors of electrodes; unmodified GE, CNF-Chi/GE, GNPs/CNF-Chi/GE, GNPs/CNF-Chi/GEs linked with the aptamer were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). After the optimization of experimental conditions (e.g., CNF concentration, pH, and incubation time), electrochemical detection of Salmonella was performed via differential pulse voltammetry (DPV) in methylene blue solution. The designed aptasensor exhibited a linear range of 10 to 105 (CFU/mL) with the limit of detection (LOD) 1.223 (Cfu/mL) for Salmonella. This aptasensor displayed excellent selectivity and remarkable sensitivity in terms of the detection of Salmonella enterica even in the real samples as compared to the polymerase chain reaction (PCR) technique. The constructed aptasensor is a highly sensitive sensor for the detection of Salmonella enterica and also can be tailored for various other targets.

scholarly journals Sensor Based on a Poly[2-(Dimethylamino)ethyl Methacrylate-Co-Styrene], Gold Nanoparticles, and Methylene Blue-Modified Glassy Carbon Electrode for Melamine Detection

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2850
Author(s):  
Fairouz Aberkane ◽  
Imene Abdou ◽  
Nadia Zine ◽  
Nicole Jaffrezic-Renault ◽  
Abdelhamid Elaissari ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Gold Nanoparticles ◽  
Impedance Spectroscopy ◽  
Glassy Carbon Electrode ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Electrochemical Impedance ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Detection Range

Melamine has been used as a non-protein nitrogenous additive in food products to artificially increase the apparent “false” protein content. Melamine is known as a dangerous and poisonous substance for human health and it causes diverse diseases. An electrochemical sensor for melamine detection has been developed by modification of a glassy carbon electrode using copolymer poly[DMAEMA-co-styrene], gold nanoparticles, and methylene blue. The characterization of the modified electrode was conducted using several analysis techniques including cyclic voltammetry (CV), differential pulse voltammetry (DPV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The electrochemical detection of melamine was performed by impedance spectroscopy. Obtained results revealed that the developed sensor has a large detection range from 5.0 × 10−13 to 3.8 × 10−8 M with a low detection limit of 1.8 × 10−12 M (at S/N = 3). Various interfering species such as phenol, hydroquinone, and bisphenol A have been used and their behavior on modified electrode has been studied.

Investigation of Electrochemical DNA Biosensor for Detection of Specific nuc Gene of Staphylococcus aureus Based on Gold Nanoparticles and Thiol Graphene Nanocomposite

2020 ◽  
Vol 16 ◽  
Author(s):  
Lijun Yan ◽  
Bo Shao ◽  
Xiaoping Zhang ◽  
Yanyan Niu ◽  
Wendi Dang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Electrochemical Impedance ◽  
Pcr Amplification ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Electrochemical Performances ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: Based on gold nanoparticles (AuNPs) and thiol graphene (TGR) nanocomposite modified carbon ionic liquid electrode, an electrochemical DNA biosensor was prepared to detect specific nuc gene of Staphylococcus aureus, which was further used to the analysis of PCR amplification samples from unfrozen pork. Objective: The construction of DNA biosensor with AuNPs and TGR could be used as a new sensing platform to detect specific ssDNA sequence in real biological samples. Method: Electrochemical behaviors of working electrodes were studied by cyclic voltammetry and electrochemical impedance spectroscopy with electrochemical performances of this biosensor investigated by differential pulse voltammetry. Results: This gene sensor could detect the specific nuc gene of Staphylococcus aureus in the linear concentration range from 1.0×10-15 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 4.5×10-16 mol L-1 (3σ), and it was applied to the detection of PCR amplification sample of Staphylococcus aureus with satisfactory results. Conclusion: This gene biosensor showed high sensitivity and good selectivity, wide detection range and low detection limit, which demonstrated an effective tool to detect specific nuc gene sequences of Staphylococcus aureus.

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