scholarly journals An electrochemical DNA biosensor for trace amounts of mercury ion quantification

2016 ◽  
Vol 14 (5) ◽  
pp. 808-815 ◽  
Author(s):  
Ferdaous Maâtouk ◽  
Mouna Maâtouk ◽  
Karima Bekir ◽  
Houcine Barhoumi ◽  
Abderrazak Maaref ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Mercury Ion ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Dna Oligonucleotides ◽  
Wastewater Samples ◽  
Eis Measurements

In this work we report the development of an electrochemical DNA biosensor with high sensitivity for mercury ion detection. A new matrix based on gold nanoparticles (AuNPs)-glutathione (GSH)/cysteine was investigated. The interaction between DNA oligonucleotides and Hg2+ ions followed by the formation of Thymine–Hg2+–Thymine (T–Hg2+–T) structures was quantified using different electrochemical methods. It has been shown that the electrochemical impedance spectroscopy (EIS) measurements and the differential pulse voltammetry (DPV) confirmed the specific interaction between the oligonucleotide receptor layer and the Hg2+ ions. Besides, the developed sensor exhibited high sensitivity towards mercury among some examined metal ions such as Pb2+, Cu2+ and Cd2+. As a result, a high electrochemical response and low detection limit of 50 pM were estimated in the case of Hg2+ ions. The developed DNA biosensor was applied successfully to the determination of Hg2+ions in wastewater samples.

Preparation of yolk–shell structured Ag@Cu particles and their application in high performance electrochemical sensing of p-aminobenzoic acid

2019 ◽  
Vol 97 (2) ◽  
pp. 140-146
Author(s):  
Tian Gan ◽  
Zhikai Wang ◽  
Mengru Chen ◽  
Wanqiu Fu ◽  
Haibo Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Performance ◽  
Electrochemical Impedance ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Electrochemical Sensing ◽  
High Catalytic Activity ◽  
Aminobenzoic Acid ◽  
Transmission Electron

In this work, the Ag@Cu particles with yolk–shell nanostructure was prepared by facile solvothermal method, which was modified on glassy carbon electrode (GCE) to fabricate electrochemical sensor for the convenient and fast determination of p-aminobenzoic acid (PABA). The surface morphology and electrochemical properties of the as-prepared Ag@Cu nanocomposite modified electrode were characterized by scanning electron microscopy, transmission electron microscopy, chronocoulometry, and electrochemical impedance spectroscopy. Further, the electrochemical sensing of PABA was performed on the Ag@Cu/GCE using cyclic voltammetry and differential pulse voltammetry techniques, showing high catalytic activity. Under the optimal conditions, the sensor exhibited a wide linear range, high sensitivity, and low detection limit of 0.315 μmol/L for PABA. The developed sensor was also successfully applied for PABA detection in anesthetic and cosmetics with satisfactory results.

Electrochemical DNA biosensor for analysis of wastewater samples

2002 ◽  
Vol 58 (1) ◽  
pp. 113-118 ◽  
Author(s):  
F Lucarelli ◽  
A Kicela ◽  
I Palchetti ◽  
G Marrazza ◽  
M Mascini
Keyword(s):  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Wastewater Samples

A ZnO–CNT nanocomposite based electrochemical DNA biosensor for meningitis detection

RSC Advances ◽  
2016 ◽  
Vol 6 (80) ◽  
pp. 76214-76222 ◽  
Author(s):  
Manvi Tak ◽  
Vinay Gupta ◽  
Monika Tomar
Keyword(s):  
Dna Detection ◽  
High Sensitivity ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor

The article focuses on the synthesis of ZnO and CNTs based electrochemical DNA biosensor and its application towards meningitis DNA detection with high sensitivity as well as selectivity.

A novel approach to design electrochemical aptamer-based biosensor for ultrasensitive detecting of zearalenone as a prevalent estrogenic mycotoxin

2021 ◽  
Vol 28 ◽  
Author(s):  
Shokoufeh Hassani ◽  
Armin Salek Maghsoudi ◽  
Milad Rezaei Akmal ◽  
Shahram Shoeibi ◽  
Fatemeh Ghadipasha ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Fusarium Species ◽  
High Sensitivity ◽  
High Specificity ◽  
Cost Effective ◽  
Differential Pulse ◽  
Electrode Position ◽  
Novel Approach ◽  
Modified Surface

Background: Zearalenone is a well-known estrogenic mycotoxin produced by Fusarium species, a serious threat to the agricultural and food industries worldwide. Zearalenone, with its known metabolites, are biomarkers of exposure to certain fungi, primarily through food. It has considerable toxic effects on biological systems due to its carcinogenicity, mutagenicity, renal toxicity, teratogenicity, and immunotoxicity. Introduction: This study aims to design a simple, quick, precise, and cost-effective method on a biosensor platform to evaluate the low levels of this toxin in foodstuffs and agricultural products. Methods: An aptamer-based electrochemical biosensor was introduced that utilizes screen-printed gold electrodes instead of conventional electrodes. The electrode position process was employed to develop a gold nanoparticle-modified surface to enhance the electroactive surface area. Thiolated aptamers were immobilized on the surface of gold nanoparticles, and subsequently, the blocker and analyte were added to the modified surface. In the presence of a redox probe, electrochemical characterization of differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy were used to investigate the various stages of aptasensor fabrication. Results: The proposed aptasensor for zearalenone concentration had a wide linear dynamic range covering the 0.5 pg/mL to 100 ng/mL with a 0.14 pg/mL detection limit. Moreover, this aptasensor had high specificity so that a non-specific analyte cannot negatively affect the selectivity of the aptasensor. Conclusion: Overall, due to its simple design, high sensitivity, and fast performance, this aptasensor showed a high potential for assessing zearalenone in real samples, providing a clear perspective for designing a portable and cost-effective device.

Investigation of Electrochemical DNA Biosensor for Detection of Specific nuc Gene of Staphylococcus aureus Based on Gold Nanoparticles and Thiol Graphene Nanocomposite

2020 ◽  
Vol 16 ◽  
Author(s):  
Lijun Yan ◽  
Bo Shao ◽  
Xiaoping Zhang ◽  
Yanyan Niu ◽  
Wendi Dang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Electrochemical Impedance ◽  
Pcr Amplification ◽  
Dna Biosensor ◽  
Carbon Ionic Liquid Electrode ◽  
Electrochemical Performances ◽  
Detection Range ◽  
Electrochemical Dna Biosensor

Background: Based on gold nanoparticles (AuNPs) and thiol graphene (TGR) nanocomposite modified carbon ionic liquid electrode, an electrochemical DNA biosensor was prepared to detect specific nuc gene of Staphylococcus aureus, which was further used to the analysis of PCR amplification samples from unfrozen pork. Objective: The construction of DNA biosensor with AuNPs and TGR could be used as a new sensing platform to detect specific ssDNA sequence in real biological samples. Method: Electrochemical behaviors of working electrodes were studied by cyclic voltammetry and electrochemical impedance spectroscopy with electrochemical performances of this biosensor investigated by differential pulse voltammetry. Results: This gene sensor could detect the specific nuc gene of Staphylococcus aureus in the linear concentration range from 1.0×10-15 mol L-1 to 1.0×10-6 mol L-1 with the detection limit as 4.5×10-16 mol L-1 (3σ), and it was applied to the detection of PCR amplification sample of Staphylococcus aureus with satisfactory results. Conclusion: This gene biosensor showed high sensitivity and good selectivity, wide detection range and low detection limit, which demonstrated an effective tool to detect specific nuc gene sequences of Staphylococcus aureus.

Electrochemical DNA Biosensor for the Detection of Short DNA Species by Using Compound Co(phen)32+

2011 ◽  
Vol 301-303 ◽  
pp. 213-218
Author(s):  
Cun Zhou ◽  
Bo Wen Cheng ◽  
Xiao Ping Fan ◽  
Zhao Dai ◽  
Guo Zheng ◽  
...  
Keyword(s):  
Electrochemical Techniques ◽  
Cobalt Complex ◽  
Cost Effective ◽  
Thiol Group ◽  
Differential Pulse ◽  
Dna Biosensor ◽  
Quantitative Detection ◽  
Electrochemical Dna Biosensor ◽  
Double Stranded Dna ◽  
Effective Manner

The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridization events in a more rapid, simplistic and cost-effective manner compared to conventional methods. Here, we present an electrochemical DNA biosensor for the specific and quantitative detection of single-stranded DNA (ssDNA). Probe oligonucleotides were immobilized onto gold electrodes by a 5′ end thiol-group linker. Following hybridization with the complementary DNA, the cobalt complex was electrochemically accumulated on the double-stranded DNA layer and the differential pulse voltammogram (DPV) for this electrode gave an electrochemical signal due to the redox reaction of [Co(phen)3]2+/3+ that was bound to the double-stranded DNA on the electrode, and it can sense single base-pair mismatche within the sequence of the hybridized double-stranded DNA (dsDNA).

scholarly journals Electrochemical Impedance Spectroscopy (EIS) in Food, Water, and Drug Analyses: Recent Advances and Applications

2020 ◽  
Author(s):  
Marwa El-Azazy
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
High Sensitivity ◽  
Electrochemical Technique ◽  
Water Safety ◽  
Food Contaminants ◽  
Sensitivity And Selectivity ◽  
Ac Current ◽  
Eis Measurements

Electrochemical impedance spectroscopy (EIS) is a potent electrochemical technique with a variety of applications. EIS measurements involve the application of an alternating current (AC) voltage (or current) to the system under investigation, followed by measurement of the response in the form of AC current (or voltage) as a function of frequency. By and large, EIS is an exceptionally attractive in terms of applications. Being nondestructive with a feasibility of implementation to the system to be measured and the usefulness of data obtained in characterizing the studied systems, electrochemical impedance spectroscopy has realms of applications. As food and water safety and security is becoming a universal concern, the need for a technique that can detect water and food contaminants with relatively high sensitivity and selectivity is evolving. EIS has started to realize its potential with a wide-term use in water and food analyses.

Graphene Modified Molecular Imprinted Electrochemical Sensor for Specific Recognition of Bovine Serum Albumin

2013 ◽  
Vol 709 ◽  
pp. 891-894 ◽  
Author(s):  
Feng Li ◽  
Jian Fei Xia ◽  
Zong Hua Wang ◽  
Yan Zhi Xia ◽  
Fei Fei Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Electrochemical Impedance ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Modified Glassy Carbon Electrode ◽  
Molecularly Imprinted ◽  
Active Probe ◽  
Pulse Voltammetry

A simple and efficient molecularly imprinted sensor (MIPs/GR/GCE) was firstly prepared by electropolymerization of pyrrole in the presence of bovine serum albumin (BSA) in an aqueous solution based on a graphene modified glassy carbon electrode for the selective recognition of bovine serum albumin. The prepared sensor was characterized by differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS), in which [Fe(CN)6]3−/4−was used as an electrochemical active probe. The results showed a wide linear range from 1.0 × 10-3to 1.0 × 10-9g/mL. And the imprinted biosensor indicated excellent selectivity and high sensitivity.

scholarly journals Electrochemical DNA biosensor for the multianalyte detection of HPV-16 and HPV-18 in oral and cervical lesions

2022 ◽  
Author(s):  
Thanyarat Chaibun ◽  
Patcharanin Thanasapburachot ◽  
Patutong Chatchawal ◽  
Su Yin Lee ◽  
Sirimanas Jiaranuchart ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Dna Biosensor ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Electrochemical Dna Biosensor ◽  
Cervical Cancers ◽  
Sandwich Hybridization ◽  
Reporter Probe ◽  
Multianalyte Detection ◽  
Hpv 18

Abstract Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. In this study, we developed an electrochemical DNA biosensor for detection of HPV-16 and HPV-18, which are the 2 most prevalent genotypes for development of oral and cervical cancers. The assay involves the sandwich hybridization of the HPV target to silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly specific and sensitive, with detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM to 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR /gel electrophoresis detection. The biosensor assay could be completed within 90 minutes. Due to its simplicity, rapidity and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories. [151 words]

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