scholarly journals Nanoporous Structure of Bone Matrix at Osteoporosis from Data of Atomic Force Microscopy and IR Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
A. A. Gaidash ◽  
L. N. Sinitsa ◽  
O. A. Babenko ◽  
A. A. Lugovskoy
Keyword(s):  
Water Clusters ◽  
Bone Matrix ◽  
Bulk Water ◽  
Nanoporous Structure ◽  
Osteoporotic Bone ◽  
Adhesion Forces ◽  
Mineral Phase ◽  
Interaction Forces ◽  
Force Microscopy ◽  
Atomic Force

It was found that in an osteoporotic bone the fraction of nanosized pores decreases, the mineral phase amorphizes, hydrated shells around mineralized particles of the bone matrix thicken, and adhesion forces increase. This contributes to the formation of water clusters similar to bulk water clusters compared to the healthy bone tissue and leads to the accumulation of more viscous liquid with increased intermolecular interaction forces in the pores of the bone matrix. Given this, the rates of chemical reactions proceeding in the water phase of ultrathin channels of general parts of collagen fibrils decrease. Ultimately, nanopores of collagen-apatite interfaces lose, to a certain extent, the capability of catalyzing the hydroxyapatite crystallization.

scholarly journals Surface Forces between Nanomagnetite and Silica in Aqueous Ca2+ Solutions Studied with AFM Colloidal Probe Method

2020 ◽  
Vol 4 (3) ◽  
pp. 41
Author(s):  
Illia Dobryden ◽  
Elizaveta Mensi ◽  
Allan Holmgren ◽  
Nils Almqvist
Keyword(s):  
Iron Ore ◽  
Adhesion Force ◽  
Surface Forces ◽  
Adhesion Forces ◽  
Interaction Forces ◽  
Colloidal Probe ◽  
Force Microscopy ◽  
Atomic Force ◽  
Repulsive Forces ◽  
Qualitative Changes

Dispersion and aggregation of nanomagnetite (Fe3O4) and silica (SiO2) particles are of high importance in various applications, such as biomedicine, nanoelectronics, drug delivery, flotation, and pelletization of iron ore. In directly probing nanomagnetite–silica interaction, atomic force microscopy (AFM) using the colloidal probe technique has proven to be a suitable tool. In this work, the interaction between nanomagnetite and silica particles was measured with AFM in aqueous Ca2+ solution at different pH levels. This study showed that the qualitative changes of the interaction forces with pH and Ca2+ concentrations were consistent with the results from zeta-potential measurements. The repulsion between nanomagnetite and silica was observed at alkaline pH and 1 mM Ca2+ concentration, but no repulsive forces were observed at 3 mM Ca2+ concentration. The interaction forces on approach were due to van der Waals and electrical double-layer forces. The good fitting of experimental data to the DLVO model and simulations supported this conclusion. However, contributions from non-DLVO forces should also be considered. It was shown that an increase of Ca2+ concentration from 1 to 3.3 mM led to a less pronounced decrease of adhesion force with increasing pH. A comparison of measured and calculated adhesion forces with a few contact mechanics models demonstrated an important impact of nanomagnetite layer nanoroughness.

Nanoscale adhesion forces between the fungal pathogen Candida albicans and macrophages

2016 ◽  
Vol 1 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Sofiane El-Kirat-Chatel ◽  
Yves F. Dufrêne
Keyword(s):  
Atomic Force Microscopy ◽  
Candida Albicans ◽  
Immune Cells ◽  
Fungal Pathogen ◽  
Fungal Pathogens ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

We establish atomic force microscopy as a new nanoscopy platform for quantifying the forces between fungal pathogens and immune cells.

scholarly journals A modular atomic force microscopy approach reveals a large range of hydrophobic adhesion forces among bacterial members of the leaf microbiota

2019 ◽  
Vol 13 (7) ◽  
pp. 1878-1882 ◽  
Author(s):  
Maximilian Mittelviefhaus ◽  
Daniel B. Müller ◽  
Tomaso Zambelli ◽  
Julia A. Vorholt
Keyword(s):  
Atomic Force Microscopy ◽  
Large Range ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology

Scanning ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Mohamed Yassine Amarouch ◽  
Jaouad El Hilaly ◽  
Driss Mazouzi
Keyword(s):  
Single Cell ◽  
Protein Interactions ◽  
Single Cells ◽  
Cardiac Cells ◽  
Cellular Biology ◽  
Adhesion Forces ◽  
Cell Organelles ◽  
Force Microscopy ◽  
Imaging Tool ◽  
Atomic Force

Atomic force microscopy (AFM) is a widely used imaging technique in material sciences. After becoming a standard surface-imaging tool, AFM has been proven to be useful in addressing several biological issues such as the characterization of cell organelles, quantification of DNA-protein interactions, cell adhesion forces, and electromechanical properties of living cells. AFM technique has undergone many successful improvements since its invention, including fluidic force microscopy (FluidFM), which combines conventional AFM with microchanneled cantilevers for local liquid dispensing. This technology permitted to overcome challenges linked to single-cell analyses. Indeed, FluidFM allows isolation and injection of single cells, force-controlled patch clamping of beating cardiac cells, serial weighting of micro-objects, and single-cell extraction for molecular analyses. This work aims to review the recent studies of AFM implementation in molecular and cellular biology.

scholarly journals Adhesion force mapping on wood by atomic force microscopy: influence of surface roughness and tip geometry

2016 ◽  
Vol 3 (10) ◽  
pp. 160248 ◽  
Author(s):  
X. Jin ◽  
B. Kasal
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Wood Surface ◽  
Adhesion Force ◽  
Data Interpretation ◽  
Natural Fibre ◽  
Force Distribution ◽  
Adhesion Forces ◽  
Force Microscopy ◽  
Atomic Force

This study attempts to address the interpretation of atomic force microscopy (AFM) adhesion force measurements conducted on the heterogeneous rough surface of wood and natural fibre materials. The influences of wood surface roughness, tip geometry and wear on the adhesion force distribution are examined by cyclic measurements conducted on wood surface under dry inert conditions. It was found that both the variation of tip and surface roughness of wood can widen the distribution of adhesion forces, which are essential for data interpretation. When a common Si AFM tip with nanometre size is used, the influence of tip wear can be significant. Therefore, control experiments should take the sequence of measurements into consideration, e.g. repeated experiments with used tip. In comparison, colloidal tips provide highly reproducible results. Similar average values but different distributions are shown for the adhesion measured on two major components of wood surface (cell wall and lumen). Evidence supports the hypothesis that the difference of the adhesion force distribution on these two locations was mainly induced by their surface roughness.

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