scholarly journals Functional Materials from Plant Biomass Obtained by Simultaneous Enzymatic Saccharification and Communition

2020 ◽  
Vol 32 (186) ◽  
pp. E63-E76
Author(s):  
Kazuhiro Shikinaka ◽  
Masaya Nakamura ◽  
Ronald R Navarro ◽  
Yuichiro Otsuka
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Functional Materials

scholarly journals Functional Materials from Plant Biomass Obtained by Simultaneous Enzymatic Saccharification and Communition

2020 ◽  
Vol 32 (186) ◽  
pp. J51-J62
Author(s):  
Kazuhiro Shikinaka ◽  
Masaya Nakamura ◽  
Ronald R Navarro ◽  
Yuichiro Otsuka
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Functional Materials

Biotechnological Potential of Bacterial Endoglucanase from Marine and Hypersaline Environments in Saccharification of Lignocellulosic Biomass Pre-Treated with Alkali or Ionic Liquid

2021 ◽  
Author(s):  
Abhijit Sar ◽  
Sudipto Biswas ◽  
Raju Biswas ◽  
Arijit Misra ◽  
Srikanta Pal ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Extracellular Enzymes ◽  
Plant Biomass ◽  
Halophilic Bacteria ◽  
Enzymatic Saccharification ◽  
Biofuel Production ◽  
Salt Adaptation ◽  
Rrna Gene ◽  
Generation Biofuel

Abstract In second-generation biofuel production, the recalcitrant plant biomass requires pretreatment prior to enzymatic hydrolysis. Pretreatment with alkali or ionic liquids (IL) such as 1-butyl 3-methyl immidazolium chloride ([Bmim][Cl]), are efficient but the residual salt in former, or IL in later process inhibits downstream enzymatic saccharification and thus require extensive washing. Recent studies have established IL tolerance by moderate halophilic bacteria being contributed by their general salt adaptation strategies. Objective of the present study is to examine whether the same holds true for their extracellular enzymes, and eventually select a few for future exploitation. In this direction, ten distinct endoglucanase positive (≥3 mm halo zone in congo-red cellulolytic assay) colonies were picked each from decomposed wood material of the hypersaline Sambhar Lake (SLW), in Rajasthan; and Bichitrapur (BPW) mangrove in Orissa. SLW and BPW samples had total salinities of 21.54% and 2.18%; and their isolates had optimum NaCl requirement of 10-15% and 1-5% respectively. The extracellular endoglucanase of SLW isolates were active in 5-25% NaCl but those from BPW remain active in only up to 5% NaCl. Interestingly, SLW endoglucanases also performed better in 10% and some even in 30% (v/v)[Bmim][Cl]. Endoglucanase secreted by two SLW isolates, identified by their 16S rRNA gene sequence as Salipaludibacillus sp. were effectively used for in situ enzymatic hydrolysis of both alkali and [Bmim][Cl] pretreated rice straw. However, endoglucanase from BPW isolate Salinicola sp. could hydrolyze seawater washed alkali-pretreated biomass thereby expanding their industrial applicability in coastal areas.

scholarly journals Organosolv pretreatment of plant biomass for enhanced enzymatic saccharification

2016 ◽  
Vol 18 (2) ◽  
pp. 360-381 ◽  
Author(s):  
Zhanying Zhang ◽  
Mark D. Harrison ◽  
Darryn W. Rackemann ◽  
William O. S. Doherty ◽  
Ian M. O'Hara
Keyword(s):  
Lignocellulosic Biomass ◽  
Organic Solvents ◽  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Organosolv Pretreatment ◽  
Different Types

Pretreatments of lignocellulosic biomass for enhanced enzymatic saccharification with different types of organic solvents are compared and reviewed.

scholarly journals Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chiaki Hori ◽  
Naoki Takata ◽  
Pui Ying Lam ◽  
Yuki Tobimatsu ◽  
Soichiro Nagano ◽  
...  
Keyword(s):  
Cell Wall ◽  
Populus Tremuloides ◽  
Cell Walls ◽  
Enzymatic Degradation ◽  
Plant Biomass ◽  
Lignin Content ◽  
Enzymatic Saccharification ◽  
Wall Structure ◽  
Xylem Cell

AbstractDeveloping an efficient deconstruction step of woody biomass for biorefinery has been drawing considerable attention since its xylem cell walls display highly recalcitrance nature. Here, we explored transcriptional factors (TFs) that reduce wood recalcitrance and improve saccharification efficiency in Populus species. First, 33 TF genes up-regulated during poplar wood formation were selected as potential regulators of xylem cell wall structure. The transgenic hybrid aspens (Populus tremula × Populus tremuloides) overexpressing each selected TF gene were screened for in vitro enzymatic saccharification. Of these, four transgenic seedlings overexpressing previously uncharacterized TF genes increased total glucan hydrolysis on average compared to control. The best performing lines overexpressing Pt × tERF123 and Pt × tZHD14 were further grown to form mature xylem in the greenhouse. Notably, the xylem cell walls exhibited significantly increased total xylan hydrolysis as well as initial hydrolysis rates of glucan. The increased saccharification of Pt × tERF123-overexpressing lines could reflect the improved balance of cell wall components, i.e., high cellulose and low xylan and lignin content, which could be caused by upregulation of cellulose synthase genes upon the expression of Pt × tERF123. Overall, we successfully identified Pt × tERF123 and Pt × tZHD14 as effective targets for reducing cell wall recalcitrance and improving the enzymatic degradation of woody plant biomass.

Analysis of Plant Biomass Pretreatment Technology for Fuel Production

2021 ◽  
Vol 15 (4) ◽  
pp. 435-448
Author(s):  
Lili Zhang ◽  
Mengdi Zhao ◽  
Kai Xiao ◽  
Xianli Liu ◽  
Xude Zhao ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Structural Characteristics ◽  
Complex Structure ◽  
Enzymatic Saccharification ◽  
Cost Effective ◽  
Biomass Energy ◽  
Raw Material ◽  
Energy Field ◽  
World Energy ◽  
Pretreatment Technology

Facing the double challenges of energy shortage and environmental pollution, development and utilization of biomass energy is of great urgency. In this review paper, the current situation and types of biomass energy are reviewed and discussed based on the world energy crisis and its environmental problems. As a typical renewable green liquid energy, bio-ethanol, made from plants with rich reserves, could be applied to the field of traffic transportation. Due to the complex structure, variety, and stable nature of plant biomass, cost-effective pretreatment technology has become an urgent demand in the bio-energy field of current world. At present, various pretreatment methods for enzymatic saccharification of plant biomass have different defects. Therefore, the research on pretreatment technology needs to comprehensively consider the heterogeneity of chemical composition and structural characteristics of raw material and the pertinence of pretreatment methods.

Mycotechnology for Lignocellulosic Bioethanol Production

2018 ◽  
pp. 28-43 ◽  
Author(s):  
Raj Kumar Pandey ◽  
Lakshmi Tewari
Keyword(s):  
Fossil Fuels ◽  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Treatment Technology ◽  
Cellulose Accessibility ◽  
Wide Range ◽  
The World ◽  
Pre Treatment ◽  
Enzymatic Delignification ◽  
Significant Production

One of the major challenges for society in 21st century is to find a sustainable eco-friendly renewable liquid fuel for replacing petroleum based fossil fuels. Bioethanol is one ofthe most consumable biofuel in the world. Lignocellulosic plant biomass can be an untapped source of fermentable sugars for significant production of bioethanol. But, the polyphenolic lignin of the biomass hinders the digestibility of cellulose, thus the goal of any pre-treatment technology is to remove this structural component to improve the cellulose accessibility for enzymatic saccharification. A wide range of pretreatment methods and their combinations have been reported for delignification, but recently, the environment friendly approach of microbial pre-treatment has received much attention for enzymatic delignification and saccharificaton of biomass. The extracellular lignin degrading enzymes and cellulase enzyme complex from fungi are now considered for biological delignification and saccharification, respectively.

Effects of fungal pretreatment and steam explosion pretreatment on enzymatic saccharification of plant biomass

1995 ◽  
Vol 48 (6) ◽  
pp. 719-724 ◽  
Author(s):  
Tatsuro Sawada ◽  
Yoshitoshi Nakamura ◽  
Fumihisa Kobayashi ◽  
Masaaki Kuwahara ◽  
Takashi Watanabe
Keyword(s):  
Steam Explosion ◽  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Steam Explosion Pretreatment ◽  
Fungal Pretreatment

scholarly journals Microbial Glucuronoyl Esterases: 10 Years after Discovery

2016 ◽  
Vol 82 (24) ◽  
pp. 7014-7018 ◽  
Author(s):  
Peter Biely
Keyword(s):  
Plant Biomass ◽  
Current Knowledge ◽  
Schizophyllum Commune ◽  
Enzymatic Saccharification ◽  
Artificial Substrates ◽  
Content Type ◽  
New Family ◽  
Biomass Processing ◽  
Hydrolysis Of ◽  
Positive Effect

ABSTRACTA carbohydrate esterase called glucuronoyl esterase (GE) was discovered 10 years ago in a cellulolytic system of the wood-rotting fungusSchizophyllum commune. Genes coding for GEs were subsequently found in a number of microbial genomes, and a new family of carbohydrate esterases (CE15) has been established. The multidomain structures of GEs, together with their catalytic properties on artificial substrates and positive effect on enzymatic saccharification of plant biomass, led to the view that the esterases evolved for hydrolysis of the ester linkages between 4-O-methyl-d-glucuronic acid of plant glucuronoxylans and lignin alcohols, one of the crosslinks in the plant cell walls. This idea of the function of GEs is further supported by the effects of cloning of fungal GEs in plants and by very recently reported evidence for changes in the size of isolated lignin-carbohydrate complexes due to uronic acid de-esterification. These facts make GEs interesting candidates for biotechnological applications in plant biomass processing and genetic modification of plants. This article is a brief summary of current knowledge of these relatively recent and unexplored esterases.

ChemInform Abstract: Organosolv Pretreatment of Plant Biomass for Enhanced Enzymatic Saccharification

ChemInform ◽  
2016 ◽  
Vol 47 (12) ◽  
pp. no-no
Author(s):  
Zhanying Zhang ◽  
Mark D. Harrison ◽  
Darryn W. Rackemann ◽  
William O. S. Doherty ◽  
Ian M. O'Hara
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Organosolv Pretreatment
Sign in / Sign up

Export Citation Format

Share Document