ChemInform Abstract: Organosolv Pretreatment of Plant Biomass for Enhanced Enzymatic Saccharification

ChemInform ◽  
2016 ◽  
Vol 47 (12) ◽  
pp. no-no
Author(s):  
Zhanying Zhang ◽  
Mark D. Harrison ◽  
Darryn W. Rackemann ◽  
William O. S. Doherty ◽  
Ian M. O'Hara
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Organosolv Pretreatment

scholarly journals Organosolv pretreatment of plant biomass for enhanced enzymatic saccharification

2016 ◽  
Vol 18 (2) ◽  
pp. 360-381 ◽  
Author(s):  
Zhanying Zhang ◽  
Mark D. Harrison ◽  
Darryn W. Rackemann ◽  
William O. S. Doherty ◽  
Ian M. O'Hara
Keyword(s):  
Lignocellulosic Biomass ◽  
Organic Solvents ◽  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Organosolv Pretreatment ◽  
Different Types

Pretreatments of lignocellulosic biomass for enhanced enzymatic saccharification with different types of organic solvents are compared and reviewed.

scholarly journals Functional Materials from Plant Biomass Obtained by Simultaneous Enzymatic Saccharification and Communition

2020 ◽  
Vol 32 (186) ◽  
pp. E63-E76
Author(s):  
Kazuhiro Shikinaka ◽  
Masaya Nakamura ◽  
Ronald R Navarro ◽  
Yuichiro Otsuka
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Functional Materials

Biotechnological Potential of Bacterial Endoglucanase from Marine and Hypersaline Environments in Saccharification of Lignocellulosic Biomass Pre-Treated with Alkali or Ionic Liquid

2021 ◽  
Author(s):  
Abhijit Sar ◽  
Sudipto Biswas ◽  
Raju Biswas ◽  
Arijit Misra ◽  
Srikanta Pal ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Extracellular Enzymes ◽  
Plant Biomass ◽  
Halophilic Bacteria ◽  
Enzymatic Saccharification ◽  
Biofuel Production ◽  
Salt Adaptation ◽  
Rrna Gene ◽  
Generation Biofuel

Abstract In second-generation biofuel production, the recalcitrant plant biomass requires pretreatment prior to enzymatic hydrolysis. Pretreatment with alkali or ionic liquids (IL) such as 1-butyl 3-methyl immidazolium chloride ([Bmim][Cl]), are efficient but the residual salt in former, or IL in later process inhibits downstream enzymatic saccharification and thus require extensive washing. Recent studies have established IL tolerance by moderate halophilic bacteria being contributed by their general salt adaptation strategies. Objective of the present study is to examine whether the same holds true for their extracellular enzymes, and eventually select a few for future exploitation. In this direction, ten distinct endoglucanase positive (≥3 mm halo zone in congo-red cellulolytic assay) colonies were picked each from decomposed wood material of the hypersaline Sambhar Lake (SLW), in Rajasthan; and Bichitrapur (BPW) mangrove in Orissa. SLW and BPW samples had total salinities of 21.54% and 2.18%; and their isolates had optimum NaCl requirement of 10-15% and 1-5% respectively. The extracellular endoglucanase of SLW isolates were active in 5-25% NaCl but those from BPW remain active in only up to 5% NaCl. Interestingly, SLW endoglucanases also performed better in 10% and some even in 30% (v/v)[Bmim][Cl]. Endoglucanase secreted by two SLW isolates, identified by their 16S rRNA gene sequence as Salipaludibacillus sp. were effectively used for in situ enzymatic hydrolysis of both alkali and [Bmim][Cl] pretreated rice straw. However, endoglucanase from BPW isolate Salinicola sp. could hydrolyze seawater washed alkali-pretreated biomass thereby expanding their industrial applicability in coastal areas.

scholarly journals Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chiaki Hori ◽  
Naoki Takata ◽  
Pui Ying Lam ◽  
Yuki Tobimatsu ◽  
Soichiro Nagano ◽  
...  
Keyword(s):  
Cell Wall ◽  
Populus Tremuloides ◽  
Cell Walls ◽  
Enzymatic Degradation ◽  
Plant Biomass ◽  
Lignin Content ◽  
Enzymatic Saccharification ◽  
Wall Structure ◽  
Xylem Cell

AbstractDeveloping an efficient deconstruction step of woody biomass for biorefinery has been drawing considerable attention since its xylem cell walls display highly recalcitrance nature. Here, we explored transcriptional factors (TFs) that reduce wood recalcitrance and improve saccharification efficiency in Populus species. First, 33 TF genes up-regulated during poplar wood formation were selected as potential regulators of xylem cell wall structure. The transgenic hybrid aspens (Populus tremula × Populus tremuloides) overexpressing each selected TF gene were screened for in vitro enzymatic saccharification. Of these, four transgenic seedlings overexpressing previously uncharacterized TF genes increased total glucan hydrolysis on average compared to control. The best performing lines overexpressing Pt × tERF123 and Pt × tZHD14 were further grown to form mature xylem in the greenhouse. Notably, the xylem cell walls exhibited significantly increased total xylan hydrolysis as well as initial hydrolysis rates of glucan. The increased saccharification of Pt × tERF123-overexpressing lines could reflect the improved balance of cell wall components, i.e., high cellulose and low xylan and lignin content, which could be caused by upregulation of cellulose synthase genes upon the expression of Pt × tERF123. Overall, we successfully identified Pt × tERF123 and Pt × tZHD14 as effective targets for reducing cell wall recalcitrance and improving the enzymatic degradation of woody plant biomass.

scholarly journals Functional Materials from Plant Biomass Obtained by Simultaneous Enzymatic Saccharification and Communition

2020 ◽  
Vol 32 (186) ◽  
pp. J51-J62
Author(s):  
Kazuhiro Shikinaka ◽  
Masaya Nakamura ◽  
Ronald R Navarro ◽  
Yuichiro Otsuka
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Functional Materials

Analysis of Plant Biomass Pretreatment Technology for Fuel Production

2021 ◽  
Vol 15 (4) ◽  
pp. 435-448
Author(s):  
Lili Zhang ◽  
Mengdi Zhao ◽  
Kai Xiao ◽  
Xianli Liu ◽  
Xude Zhao ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Structural Characteristics ◽  
Complex Structure ◽  
Enzymatic Saccharification ◽  
Cost Effective ◽  
Biomass Energy ◽  
Raw Material ◽  
Energy Field ◽  
World Energy ◽  
Pretreatment Technology

Facing the double challenges of energy shortage and environmental pollution, development and utilization of biomass energy is of great urgency. In this review paper, the current situation and types of biomass energy are reviewed and discussed based on the world energy crisis and its environmental problems. As a typical renewable green liquid energy, bio-ethanol, made from plants with rich reserves, could be applied to the field of traffic transportation. Due to the complex structure, variety, and stable nature of plant biomass, cost-effective pretreatment technology has become an urgent demand in the bio-energy field of current world. At present, various pretreatment methods for enzymatic saccharification of plant biomass have different defects. Therefore, the research on pretreatment technology needs to comprehensively consider the heterogeneity of chemical composition and structural characteristics of raw material and the pertinence of pretreatment methods.

scholarly journals Organosolv Fractionation of Birch Sawdust: Establishing a Lignin-First Biorefinery

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6754
Author(s):  
Maxwel Monção ◽  
Kateřina Hrůzová ◽  
Ulrika Rova ◽  
Leonidas Matsakas ◽  
Paul Christakopoulos
Keyword(s):  
Enzymatic Saccharification ◽  
Value Added ◽  
Pilot Scale ◽  
Cellulose Content ◽  
Paper Pulp ◽  
Organosolv Pretreatment ◽  
Residual Biomass ◽  
Main Challenge ◽  
Different Temperatures ◽  
Pretreated Biomass

The use of residual biomass for bioconversions makes it possible to decrease the output of fossil-based chemicals and pursue a greener economy. While the use of lignocellulosic material as sustainable feedstock has been tried at pilot scale, industrial production is not yet economically feasible, requiring further technology and feedstock optimization. The aim of this study was to examine the feasibility of replacing woodchips with residual sawdust in biorefinery applications. Woodchips can be used in value-added processes such as paper pulp production, whereas sawdust is currently used mainly for combustion. The main advantages of sawdust are its large supply and a particle size sufficiently small for the pretreatment process. Whereas, the main challenge is the higher complexity of the lignocellulosic biomass, as it can contain small amounts of bark and cambium. Here, we studied the fractionation of birch sawdust by organosolv pretreatment at two different temperatures and for two different durations. We evaluated the efficiency of fractionation into the three main fractions: lignin, cellulose, and hemicellulose. The cellulose content in pretreated biomass was as high as 69.2%, which was nearly double the amount in untreated biomass. The obtained lignin was of high purity, with a maximum 4.5% of contaminating sugars. Subsequent evaluation of the susceptibility of pretreated solids to enzymatic saccharification revealed glucose yields ranging from 75% to 90% after 48 h but reaching 100.0% under the best conditions. In summary, birch sawdust can be successfully utilized as a feedstock for organosolv fractionation and replace woodchips to simplify and lower the costs of biorefinery processes.

scholarly journals Effects of Organosolv Pretreatment Using Temperature-Controlled Bench-Scale Ball Milling on Enzymatic Saccharification of Miscanthus × giganteus

Energies ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 2657 ◽  
Author(s):  
Tae Kim ◽  
Dongjoong Im ◽  
Kyeong Oh ◽  
Tae Kim
Keyword(s):  
Reaction Time ◽  
Ethanol Concentration ◽  
Enzymatic Saccharification ◽  
Compositional Analysis ◽  
Organosolv Pretreatment ◽  
Enzymatic Digestibility ◽  
Bench Scale ◽  
Solid Ratio ◽  
Miscanthus Giganteus ◽  
Pretreatment Conditions

The effect of organosolv pretreatment was investigated using a 30 L bench-scale ball mill reactor that was capable of simultaneously performing physical and chemical pretreatment. Various reaction conditions were tried in order to discover the optimal conditions for the minimal cellulose loss and enhanced enzymatic digestibility of Miscanthus × giganteus (MG), with conditions varying from room temperature to 170 °C for reaction temperature, from 30 to 120 min of reaction time, from 30% to 60% ethanol concentration, and a liquid/solid ratio (L/S) of 10–20 under non-catalyst conditions. The pretreatment effects were evaluated by chemical compositional analysis, enzymatic digestibility test and X-ray diffraction of the treated samples. The pretreatment conditions for the highest glucan digestibility yield were determined as 170 °C, reaction time of 90 min, ethanol concentration of 40% and L/S = 10. With these pretreatment conditions, the XMG (xylan + mannan + galactan) fractionation yield and delignification were 84.4% and 53.2%, respectively. The glucan digestibility of treated MG after the aforementioned pretreatment conditions was 86.0% with 15 filter paper units (FPU) of cellulase (Cellic® CTec2) per g-glucan enzyme loading.

Mycotechnology for Lignocellulosic Bioethanol Production

2018 ◽  
pp. 28-43 ◽  
Author(s):  
Raj Kumar Pandey ◽  
Lakshmi Tewari
Keyword(s):  
Fossil Fuels ◽  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Treatment Technology ◽  
Cellulose Accessibility ◽  
Wide Range ◽  
The World ◽  
Pre Treatment ◽  
Enzymatic Delignification ◽  
Significant Production

One of the major challenges for society in 21st century is to find a sustainable eco-friendly renewable liquid fuel for replacing petroleum based fossil fuels. Bioethanol is one ofthe most consumable biofuel in the world. Lignocellulosic plant biomass can be an untapped source of fermentable sugars for significant production of bioethanol. But, the polyphenolic lignin of the biomass hinders the digestibility of cellulose, thus the goal of any pre-treatment technology is to remove this structural component to improve the cellulose accessibility for enzymatic saccharification. A wide range of pretreatment methods and their combinations have been reported for delignification, but recently, the environment friendly approach of microbial pre-treatment has received much attention for enzymatic delignification and saccharificaton of biomass. The extracellular lignin degrading enzymes and cellulase enzyme complex from fungi are now considered for biological delignification and saccharification, respectively.

scholarly journals Ammonia-ethanol-water pretreatment of wheat straw for facilitating enzymatic saccharification integrated with the preparation of submicron lignin spheres

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 5087-5109
Author(s):  
Xihui Zhang ◽  
Guang Yu ◽  
Xiaoyan Feng ◽  
Zhenqiu Li ◽  
Bin Li ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Ethanol Concentration ◽  
Hollow Structure ◽  
Enzymatic Saccharification ◽  
Average Diameter ◽  
Ammonia Concentration ◽  
Organosolv Pretreatment ◽  
Water Pretreatment ◽  
Pretreatment Conditions ◽  
Pretreated Wheat Straw

Ammonia-ethanol-water (AEW) pretreatment was adopted to treat wheat straw for ameliorating saccharification with the integrated preparation of submicron lignin spheres (SLS). Results showed that AEW pretreatment could remove 77% of lignin and 90% of extractives, thus increasing specific surface area and porosity of the substrate and finally enhancing the release of fermentable sugars in saccharification. Under the optimal pretreatment conditions (170 °C for 2 h, ethanol concentration 55% (v/v), ammonia concentration 7.5 wt%), the final total sugar yield reached 81.7% after pretreatment and saccharification, which was 2.25 times higher compared to the conventional ethanol organosolv pretreatment. Moreover, washing could be excluded for AEW-pretreated wheat straw before saccharification, and both ethanol and ammonia could be readily recovered and reused, making the AEW pretreatment clean and sustainable. In addition, SLS with hollow structure and average diameter of 161.2 ± 53.6 nm were fabricated using the fractionated lignin, which could offset the overall cost of AEW pretreatment.

Sign in / Sign up

Export Citation Format

Share Document