IFNs Reset the Differential Capacity of Human Monocyte Subsets to Produce IL-12 in Response to Microbial Stimulation

2021 ◽  
Vol 206 (7) ◽  
pp. 1642-1652
Author(s):  
Alice Muglia Amancio ◽  
Lara Mittereder ◽  
Alexie Carletti ◽  
Kevin W. Tosh ◽  
Daniel Green ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Monocyte Subsets ◽  
Differential Capacity

The three human monocyte subsets: implications for health and disease

2012 ◽  
Vol 53 (1-3) ◽  
pp. 41-57 ◽  
Author(s):  
Kok Loon Wong ◽  
Wei Hseun Yeap ◽  
June Jing Yi Tai ◽  
Siew Min Ong ◽  
Truong Minh Dang ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Monocyte Subsets ◽  
Health And Disease

scholarly journals Protease-Activated Receptors 1 and 3 are Differentially Expressed on Human Monocyte Subsets and are Upregulated by Lipopolysaccharide Ex Vivo and In Vivo

2019 ◽  
Vol 119 (09) ◽  
pp. 1394-1402 ◽  
Author(s):  
Barbara Thaler ◽  
Philipp J. Hohensinner ◽  
Johanna Baumgartner ◽  
Patrick Haider ◽  
Konstantin A. Krychtiuk ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Critical Role ◽  
Human Monocyte ◽  
In Vivo Model ◽  
Monocyte Subsets ◽  
Plasminogen Activator Inhibitor 1 ◽  
Protease Activated Receptors ◽  
Messenger Ribonucleic Acid ◽  
Pai 1

AbstractMonocytes are activated in inflammatory conditions via a variety of cytokine receptors as well as in a procoagulatory setting through thrombin, acting upon protease-activated receptors (PARs). This study investigated the expression pattern of PAR1 and PAR3 on human monocyte subsets. Furthermore, a possible regulation of the expression of PAR1 and PAR3 in these cells by inflammatory activation were studied. CD16+ monocytes showed significantly higher levels of PAR1 and PAR3 as compared with CD16− monocytes. Ex vivo treatment of whole blood with lipopolysaccharide (LPS) increased PAR1 and PAR3 messenger ribonucleic acid (mRNA) in human monocytes. In addition, increase of PAR1 was seen in all three subsets upon LPS treatment, whereas PAR3 increased significantly only in CD16− monocytes and nonclassical CD16+ monocytes. Protein levels of PAR1 and PAR3 significantly increased on monocytes in vivo in human endotoxemia 1 hour after LPS infusion. PAR1 increased significantly in CD16− monocytes and nonclassical CD16+ monocytes. In this in vivo model, PAR3 was also significantly elevated in CD16− monocytes and increased slightly albeit not significantly in CD16+ monocytes. Endotoxemia increased plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF) expression in monocytes in humans. Pretreatment of healthy volunteers with the PAR1 antagonist vorapaxar blocked the increase in PAI-1 but not the increase in TF. We here provide new evidence for a critical role for monocytes as cellular mediators that contribute to the activation of coagulation in diseases characterized by an inflammatory state.

Differential Tumor Necrosis Factor Production by Human Monocyte Subsets

1990 ◽  
Vol 47 (3) ◽  
pp. 206-216 ◽  
Author(s):  
Gyongyi Szabo ◽  
Carol L. Miller-Graziano ◽  
Jia-Yan Wu ◽  
Thomas Takayama ◽  
Karen Kodys
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Human Monocyte ◽  
Monocyte Subsets ◽  
Tumor Necrosis Factor Production ◽  
Factor Production ◽  
Necrosis Factor

scholarly journals Human Monocyte Subsets at Homeostasis and Their Perturbation in Numbers and Function in Filarial Infection

2014 ◽  
Vol 82 (11) ◽  
pp. 4438-4446 ◽  
Author(s):  
Roshanak Tolouei Semnani ◽  
Vanessa Moore ◽  
Sasisekhar Bennuru ◽  
Renee McDonald-Fleming ◽  
Sundar Ganesan ◽  
...  
Keyword(s):  
Wuchereria Bancrofti ◽  
Human Monocyte ◽  
Interleukin 10 ◽  
Alternative Activation ◽  
Helminth Parasites ◽  
Monocyte Subsets ◽  
Filarial Infection ◽  
Content Type ◽  
And Function

ABSTRACTTo characterize the function and plasticity of the major human circulating monocyte populations and to explore their role in systemic helminth infection, highly purified (by flow-based sorting) human monocyte subsets (CD14hi/CD16neg[classical], CD14+ or hi/CD16med[intermediate], and CD14neg/CD16hi[nonclassical]) were examined at homeostasis and after activation. Among these three subsets the classical and intermediate subsets were found to be the major sources of inflammatory and regulatory cytokines, as well as cytokines/chemokines associated with alternative activation, whereas the nonclassical and classical populations demonstrated an ability to transmigrate through endothelial monolayers. Moreover, it was primarily the classical subset that was the most efficient in promoting autologous T cell proliferation. The distribution of these subsets changed in the context of a systemic helminth (Wuchereria bancrofti) infection such that patent infection altered the frequency and distribution of these monocyte subsets with the nonclassical monocytes being expanded (almost 2-fold) in filarial infection. To understand further the filarial/monocyte interface,in vitromodeling demonstrated that the classical subset internalized filarial antigens more efficiently than the other two subsets but that the parasite-driven regulatory cytokine interleukin-10 was exclusively coming from the intermediate subset. Our data suggest that monocyte subsets have a differential function at homeostasis and in response to helminth parasites.

scholarly journals Phenotype, function, and differentiation potential of human monocyte subsets

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0176460 ◽  
Author(s):  
Lisa B. Boyette ◽  
Camila Macedo ◽  
Kevin Hadi ◽  
Beth D. Elinoff ◽  
John T. Walters ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Monocyte Subsets ◽  
Differentiation Potential

Diverging biological roles among human monocyte subsets in the context of tuberculosis infection

2015 ◽  
Vol 129 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Luciana Balboa ◽  
Jorge Barrios-Payan ◽  
Erika González-Domínguez ◽  
Claire Lastrucci ◽  
Geanncarlo Lugo-Villarino ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Monocyte ◽  
Tuberculosis Infection ◽  
Monocyte Subsets

We demonstrated divergent roles of human monocyte subsets throughout the course of the M. tuberculosis infection. Whereas CD16neg monocytes may contribute to the anti-mycobacterial immune response, CD16pos monocytes might promote microbial resilience.

Differential expression of Toll-like receptor 4 and human monocyte subsets in acute myocardial infarction

2012 ◽  
Vol 221 (1) ◽  
pp. 249-253 ◽  
Author(s):  
Manabu Kashiwagi ◽  
Toshio Imanishi ◽  
Yuichi Ozaki ◽  
Keisuke Satogami ◽  
Tomizo Masuno ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Differential Expression ◽  
Human Monocyte ◽  
Toll Like Receptor ◽  
Monocyte Subsets ◽  
Toll Like Receptor 4

scholarly journals Heterogeneity in the Locomotory Behavior of Human Monocyte Subsets over Human Vascular Endothelium In Vitro

2015 ◽  
Vol 195 (3) ◽  
pp. 1162-1170 ◽  
Author(s):  
Joanna L. Collison ◽  
Leo M. Carlin ◽  
Martin Eichmann ◽  
Frederic Geissmann ◽  
Mark Peakman
Keyword(s):  
Vascular Endothelium ◽  
Human Monocyte ◽  
Monocyte Subsets ◽  
Locomotory Behavior
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