Diverging biological roles among human monocyte subsets in the context of tuberculosis infection

2015 ◽  
Vol 129 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Luciana Balboa ◽  
Jorge Barrios-Payan ◽  
Erika González-Domínguez ◽  
Claire Lastrucci ◽  
Geanncarlo Lugo-Villarino ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Monocyte ◽  
Tuberculosis Infection ◽  
Monocyte Subsets

We demonstrated divergent roles of human monocyte subsets throughout the course of the M. tuberculosis infection. Whereas CD16neg monocytes may contribute to the anti-mycobacterial immune response, CD16pos monocytes might promote microbial resilience.

scholarly journals Deletion of N-acetylmuramyl-L-alanine amidases alters the host immune response to Mycobacterium tuberculosis infection

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1227-1238
Author(s):  
Nathan Scott Kieswetter ◽  
Mumin Ozturk ◽  
Shelby-Sara Jones ◽  
Sibusiso Senzani ◽  
Melissa Dalcina Chengalroyen ◽  
...  
Keyword(s):  
Immune Response ◽  
Mycobacterium Tuberculosis ◽  
Host Immune Response ◽  
Tuberculosis Infection ◽  
Mycobacterium Tuberculosis Infection

scholarly journals Severe Traumatic Injury Induces Phenotypic and Functional Changes of Neutrophils and Monocytes

2021 ◽  
Vol 10 (18) ◽  
pp. 4139
Author(s):  
Andrea Janicova ◽  
Nils Becker ◽  
Baolin Xu ◽  
Marija Simic ◽  
Laurens Noack ◽  
...  
Keyword(s):  
Immune Response ◽  
Traumatic Injury ◽  
Physiological Role ◽  
Blood Analysis ◽  
Intermediate Phenotype ◽  
Ros Generation ◽  
Monocyte Subsets ◽  
Phagocytic Capacity ◽  
Functional Changes ◽  
Severe Traumatic Injury

Background: Severe traumatic injury has been associated with high susceptibility for the development of secondary complications caused by dysbalanced immune response. As the first line of the cellular immune response, neutrophils and monocytes recruited to the site of tissue damage and/or infection, are divided into three different subsets according to their CD16/CD62L and CD16/CD14 expression, respectively. Their differential functions have not yet been clearly understood. Thus, we evaluated the phenotypic changes of neutrophil and monocyte subsets among their functionality regarding oxidative burst and the phagocytic capacity in severely traumatized patients. Methods: Peripheral blood was withdrawn from severely injured trauma patients (TP; n = 15, ISS ≥ 16) within the first 12 h post-trauma and from healthy volunteers (HV; n = 15) and stimulated with fMLP and PMA. CD16dimCD62Lbright (immature), CD16brightCD62Lbright (mature) and CD16brightCD62Ldim (CD62Llow) neutrophil subsets and CD14brightCD16− (classical), CD14brightCD16+ (intermediate) and CD14dimCD16+ (non-classical) monocyte subsets of HV and TP were either directly analyzed by flow cytometry or the examined subsets of HV were sorted first by fluorescence-activated cell sorting and subsequently analyzed. Subset-specific generation of reactive oxygen species (ROS) and of E. coli bioparticle phagocytosis were evaluated. Results: In TP, the counts of immature neutrophils were significantly increased vs. HV. The numbers of mature and CD62Ldim neutrophils remained unchanged but the production of ROS was significantly enhanced in TP vs. HV and the stimulation with fMLP significantly increased the generation of ROS in the mature and CD62Ldim neutrophils of HV. The counts of phagocyting neutrophils did not change but the mean phagocytic capacity showed an increasing trend in TP. In TP, the monocytes shifted toward the intermediate phenotype, whereas the classical and non-classical monocytes became less abundant. ROS generation was significantly increased in all monocyte subsets in TP vs. HV and PMA stimulation significantly increased those level in both, HV and TP. However, the PMA-induced mean ROS generation was significantly lower in intermediate monocytes of TP vs. HV. Sorting of monocyte and neutrophil subsets revealed a significant increase of ROS and decrease of phagocytic capacity vs. whole blood analysis. Conclusions: Neutrophils and monocytes display a phenotypic shift following severe injury. The increased functional abnormalities of certain subsets may contribute to the dysbalanced immune response and attenuate the antimicrobial function and thus, may represent a potential therapeutic target. Further studies on isolated subsets are necessary for evaluation of their physiological role after severe traumatic injury.

The three human monocyte subsets: implications for health and disease

2012 ◽  
Vol 53 (1-3) ◽  
pp. 41-57 ◽  
Author(s):  
Kok Loon Wong ◽  
Wei Hseun Yeap ◽  
June Jing Yi Tai ◽  
Siew Min Ong ◽  
Truong Minh Dang ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Monocyte Subsets ◽  
Health And Disease

scholarly journals Protease-Activated Receptors 1 and 3 are Differentially Expressed on Human Monocyte Subsets and are Upregulated by Lipopolysaccharide Ex Vivo and In Vivo

2019 ◽  
Vol 119 (09) ◽  
pp. 1394-1402 ◽  
Author(s):  
Barbara Thaler ◽  
Philipp J. Hohensinner ◽  
Johanna Baumgartner ◽  
Patrick Haider ◽  
Konstantin A. Krychtiuk ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Critical Role ◽  
Human Monocyte ◽  
In Vivo Model ◽  
Monocyte Subsets ◽  
Plasminogen Activator Inhibitor 1 ◽  
Protease Activated Receptors ◽  
Messenger Ribonucleic Acid ◽  
Pai 1

AbstractMonocytes are activated in inflammatory conditions via a variety of cytokine receptors as well as in a procoagulatory setting through thrombin, acting upon protease-activated receptors (PARs). This study investigated the expression pattern of PAR1 and PAR3 on human monocyte subsets. Furthermore, a possible regulation of the expression of PAR1 and PAR3 in these cells by inflammatory activation were studied. CD16+ monocytes showed significantly higher levels of PAR1 and PAR3 as compared with CD16− monocytes. Ex vivo treatment of whole blood with lipopolysaccharide (LPS) increased PAR1 and PAR3 messenger ribonucleic acid (mRNA) in human monocytes. In addition, increase of PAR1 was seen in all three subsets upon LPS treatment, whereas PAR3 increased significantly only in CD16− monocytes and nonclassical CD16+ monocytes. Protein levels of PAR1 and PAR3 significantly increased on monocytes in vivo in human endotoxemia 1 hour after LPS infusion. PAR1 increased significantly in CD16− monocytes and nonclassical CD16+ monocytes. In this in vivo model, PAR3 was also significantly elevated in CD16− monocytes and increased slightly albeit not significantly in CD16+ monocytes. Endotoxemia increased plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF) expression in monocytes in humans. Pretreatment of healthy volunteers with the PAR1 antagonist vorapaxar blocked the increase in PAI-1 but not the increase in TF. We here provide new evidence for a critical role for monocytes as cellular mediators that contribute to the activation of coagulation in diseases characterized by an inflammatory state.

Differential Tumor Necrosis Factor Production by Human Monocyte Subsets

1990 ◽  
Vol 47 (3) ◽  
pp. 206-216 ◽  
Author(s):  
Gyongyi Szabo ◽  
Carol L. Miller-Graziano ◽  
Jia-Yan Wu ◽  
Thomas Takayama ◽  
Karen Kodys
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Human Monocyte ◽  
Monocyte Subsets ◽  
Tumor Necrosis Factor Production ◽  
Factor Production ◽  
Necrosis Factor

Azithromycin modulates immune response of human monocyte-derived dendritic cells and CD4 + T cells

2016 ◽  
Vol 40 ◽  
pp. 318-326 ◽  
Author(s):  
Syh-Jae Lin ◽  
Ming-Ling Kuo ◽  
Hsiu-Shan Hsiao ◽  
Pei-Tzu Lee
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Dendritic Cells ◽  
Cd4 T Cells ◽  
Human Monocyte
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