scholarly journals IFN-γ Negatively Modulates Self-Renewal of Repopulating Human Hemopoietic Stem Cells

2005 ◽  
Vol 174 (2) ◽  
pp. 752-757 ◽  
Author(s):  
Liping Yang ◽  
Ingunn Dybedal ◽  
David Bryder ◽  
Lars Nilsson ◽  
Ewa Sitnicka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hemopoietic Stem Cells ◽  
Self Renewal ◽  
Ifn Γ

scholarly journals Committed Hemopoietic Progenitors, Not Stem Cells, are the Principal Responders to Hox Gene Transduction

2017 ◽  
Author(s):  
Harvey Lim ◽  
Salima Janmohamed ◽  
Patricia Benveniste ◽  
Robert Herrington ◽  
Mary Barbara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hox Genes ◽  
Hemopoietic Stem Cells ◽  
Gene Transduction ◽  
Hox Proteins ◽  
Self Renewal ◽  
Gene Deletions ◽  
Hox Gene ◽  
Endogenous Genes ◽  
Proliferative Lifespan

ABSTRACTAs hemopoietic stem cells differentiate, their proliferative lifespan shortens by unknown mechanisms. Homeobox cluster (Hox) genes have been implicated by their enhancement of self-renewal when transduced into hemopoietic cells, but gene deletions have been inconclusive because of functional redundancy. Here we enforced HOXB4 expression in purified precursor stages, and compared responses of early stages expressing the endogenous genes with later stages that did not. Contrary to the prevalent view that transduced Hox genes enhance the self-renewal of hemopoietic stem cells, stem cells or their multipotent progeny expressing the endogenous genes showed little response. Instead, immortalization, extensive self-renewal and acquired reconstituting potential occurred in committed erythroid and myeloid progenitors where the endogenous genes were shutting down. The results change our understanding of the stages affected by exogenous HOX proteins and point to shutdown of the endogenous genes as a principal determinant of the shortened clonal lifespans of committed progenitor cells.

The self renewal probability of hemopoietic stem cells

1968 ◽  
Vol 72 (3) ◽  
pp. 221-228 ◽  
Author(s):  
Helmut Vogel ◽  
Helgard Niewisch ◽  
Gastone Matioli
Keyword(s):  
Stem Cells ◽  
The Self ◽  
Hemopoietic Stem Cells ◽  
Self Renewal

scholarly journals Analysis of differentiation of mouse hemopoietic stem cells in culture by sequential replating of paired progenitors

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 393-399 ◽  
Author(s):  
J Suda ◽  
T Suda ◽  
M Ogawa
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Blast Cell ◽  
Hemopoietic Stem Cells ◽  
Spleen Cells ◽  
Pluripotent Cells ◽  
Self Renewal ◽  
Cell Lineages ◽  
Daughter Cells ◽  
Successive Division

Abstract Blast cell colonies seen in cultures of spleen cells from 5- fluorouracil-treated mice provide a highly enriched population of primitive hemopoietic progenitors. Our recent studies of the differentiation potentials of the paired daughter cells of these progenitors showed different patterns of differentiation in the colonies produced by the separated daughter cells. In this study, we carried out sequential micromanipulation of paired progenitors followed by cytologic examinations of the colonies derived from these progenitors. Of the total 94 evaluable cultures, consisting of three or more colonies, 52 consisted of macrophage colonies and one consisted of megakaryocyte colonies. In the remaining 41 cultures, diverse combinations of colonies revealing heterogeneous compositions of cell lineages were identified. Presumptive genealogic trees of the differentiation of hemopoietic progenitors constructed for the latter group of cultures suggested that monopotent progenitors may be derived from pluripotent progenitors in two ways: (1) directly during one cell division of pluripotent cells or (2) as a result of progressive lineage restriction during successive division of the pluripotent progenitors. The results also suggested that some of the oligopotent progenitors are capable of limited self-renewal.

scholarly journals Analysis of differentiation of mouse hemopoietic stem cells in culture by sequential replating of paired progenitors

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 393-399 ◽  
Author(s):  
J Suda ◽  
T Suda ◽  
M Ogawa
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Blast Cell ◽  
Hemopoietic Stem Cells ◽  
Spleen Cells ◽  
Pluripotent Cells ◽  
Self Renewal ◽  
Cell Lineages ◽  
Daughter Cells ◽  
Successive Division

Blast cell colonies seen in cultures of spleen cells from 5- fluorouracil-treated mice provide a highly enriched population of primitive hemopoietic progenitors. Our recent studies of the differentiation potentials of the paired daughter cells of these progenitors showed different patterns of differentiation in the colonies produced by the separated daughter cells. In this study, we carried out sequential micromanipulation of paired progenitors followed by cytologic examinations of the colonies derived from these progenitors. Of the total 94 evaluable cultures, consisting of three or more colonies, 52 consisted of macrophage colonies and one consisted of megakaryocyte colonies. In the remaining 41 cultures, diverse combinations of colonies revealing heterogeneous compositions of cell lineages were identified. Presumptive genealogic trees of the differentiation of hemopoietic progenitors constructed for the latter group of cultures suggested that monopotent progenitors may be derived from pluripotent progenitors in two ways: (1) directly during one cell division of pluripotent cells or (2) as a result of progressive lineage restriction during successive division of the pluripotent progenitors. The results also suggested that some of the oligopotent progenitors are capable of limited self-renewal.

scholarly journals Separate Control of the Survival, the Self-renewal and the Differentiation of Hemopoietic Stem Cells.

1995 ◽  
Vol 20 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Takanori Yanai ◽  
Kenkichi Sugimoto ◽  
Emi Takashita ◽  
Yohko Aihara ◽  
Youko Tsurumaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
The Self ◽  
Hemopoietic Stem Cells ◽  
Self Renewal ◽  
Separate Control

Extended self-renewal capacity of pluripotent hemopoietic stem cells: Association with persistent friend spleen focus-forming virus

Cell ◽  
1982 ◽  
Vol 31 (3) ◽  
pp. 731-738 ◽  
Author(s):  
Robert J. Eckner ◽  
Kristine L. Hettrick ◽  
Joel S. Greenberger ◽  
Michael Bennett
Keyword(s):  
Stem Cells ◽  
Hemopoietic Stem Cells ◽  
Extended Self ◽  
Self Renewal

Self-renewal capacity of murine hemopoietic stem cells under internal contamination with239Pu and241Am

1985 ◽  
Vol 24 (3) ◽  
pp. 203-209 ◽  
Author(s):  
V. Svoboda ◽  
A. Sedlák ◽  
D. Bubeníková ◽  
Z. Kotašková ◽  
O. Truxová
Keyword(s):  
Stem Cells ◽  
Hemopoietic Stem Cells ◽  
Self Renewal ◽  
Internal Contamination
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