scholarly journals High Levels of Adenosine Deaminase on Dendritic Cells Promote Autoreactive T Cell Activation and Diabetes in Nonobese Diabetic Mice

2011 ◽  
Vol 186 (12) ◽  
pp. 6798-6806 ◽  
Author(s):  
Faranak Ghaemi Oskouie ◽  
Afshin Shameli ◽  
Ailian Yang ◽  
Melanie D. Desrosiers ◽  
Ashley D. Mucsi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Adenosine Deaminase ◽  
T Cell Activation ◽  
Cell Activation ◽  
Diabetic Mice ◽  
Nonobese Diabetic ◽  
Autoreactive T Cell ◽  
Nonobese Diabetic Mice

scholarly journals Correction: Levels of Adenosine Deaminase on Dendritic Cells Promote Autoreactive T Cell Activation and Diabetes in Nonobese Diabetic Mice

2011 ◽  
Vol 187 (4) ◽  
pp. 2031-2031
Author(s):  
Faranak Ghaemi Oskouie ◽  
Afshin Shameli ◽  
Ailian Yang ◽  
Melanie D. Desrosiers ◽  
Ashley D. Mucsi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Adenosine Deaminase ◽  
T Cell Activation ◽  
Cell Activation ◽  
Diabetic Mice ◽  
Nonobese Diabetic ◽  
Autoreactive T Cell ◽  
Nonobese Diabetic Mice

scholarly journals Correction: Autoimmunity during Thymectomy-Induced Lymphopenia: Role of Thymus Ablation and Initial Effector T Cell Activation Timing in Nonobese Diabetic Mice

2010 ◽  
Vol 185 (5) ◽  
pp. 3111-3111
Author(s):  
Marie-Claude Gagnerault ◽  
Olivia Lanvin ◽  
Virginie Pasquier ◽  
Corinne Garcia ◽  
Diane Damotte ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Diabetic Mice ◽  
Effector T Cell ◽  
Nonobese Diabetic ◽  
Nonobese Diabetic Mice

scholarly journals Autoimmunity during Thymectomy-Induced Lymphopenia: Role of Thymus Ablation and Initial Effector T Cell Activation Timing in Nonobese Diabetic Mice

2009 ◽  
Vol 183 (8) ◽  
pp. 4913-4920 ◽  
Author(s):  
Marie-Claude Gagnerault ◽  
Olivia Lanvin ◽  
Virginie Pasquier ◽  
Corinne Garcia ◽  
Diane Damotte ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Diabetic Mice ◽  
Effector T Cell ◽  
Nonobese Diabetic ◽  
Nonobese Diabetic Mice

scholarly journals Differential Contributions of APC Subsets to T Cell Activation in Nonobese Diabetic Mice

2008 ◽  
Vol 180 (8) ◽  
pp. 5235-5249 ◽  
Author(s):  
Annette M. Marleau ◽  
Kelly L. Summers ◽  
Bhagirath Singh
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Diabetic Mice ◽  
Nonobese Diabetic ◽  
Nonobese Diabetic Mice

scholarly journals Development of Type 1 Diabetes Mellitus in Nonobese Diabetic Mice Follows Changes in Thymocyte and Peripheral T Lymphocyte Transcriptional Activity

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Thais A. Fornari ◽  
Paula B. Donate ◽  
Claudia Macedo ◽  
Elza T. Sakamoto-Hojo ◽  
Eduardo A. Donadi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
T Cell ◽  
T Lymphocytes ◽  
T Cell Activation ◽  
Transcriptional Activity ◽  
Cell Activation ◽  
Autoimmune Diabetes ◽  
Immune Reactivity ◽  
Diabetic Mice ◽  
Nonobese Diabetic

As early as one month of age, nonobese diabetic (NOD) mice feature pancreatic infiltration of autoreactive T lymphocytes, which destruct insulin-producing beta cells, producing autoimmune diabetes mellitus (T1D) within eight months. Thus, we hypothesized that during the development of T1D, the transcriptional modulation of immune reactivity genes may occur as thymocytes mature into peripheral T lymphocytes. The transcriptome of thymocytes and peripheral CD3+T lymphocytes from prediabetic or diabetic mice analyzed through microarray hybridizations identified 2,771 differentially expressed genes. Hierarchical clustering grouped mice according to age/T1D onset and genes according to their transcription profiling. The transcriptional activity of thymocytes developing into peripheral T lymphocytes revealed sequential participation of genes involved with CD4+/CD8+T-cell differentiation (Themis), tolerance induction by Tregs (Foxp3), and apoptosis (Fasl) soon after T-cell activation (IL4), while the emergence of T1D coincided with the expression of cytotoxicity (Crtam) and inflammatory response genes (Tlr) by peripheral T lymphocytes.

scholarly journals CD14 Expressing Precursors Give Rise to Highly Functional Conventional Dendritic Cells for Use as Dendritic Cell Vaccine

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3818
Author(s):  
Maud Plantinga ◽  
Denise A. M. H. van den Beemt ◽  
Ester Dünnebach ◽  
Stefan Nierkens
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Cord Blood ◽  
T Cell Activation ◽  
Cell Activation ◽  
Ex Vivo ◽  
Dendritic Cell Vaccine ◽  
Cell Vaccine ◽  
Activated T Cells

Induction of long-lasting immunity by dendritic cells (DCs) makes them attractive candidates for anti-tumor vaccination. Although DC vaccinations are generally considered safe, clinical responses remain inconsistent in clinical trials. This initiated studies to identify subsets of DCs with superior capabilities to induce effective and memory anti-tumor responses. The use of primary DCs has been suggested to overcome the functional limitations of ex vivo monocyte-derived DCs (moDC). The ontogeny of primary DCs has recently been revised by the introduction of DC3, which phenotypically resembles conventional (c)DC2 as well as moDC. Previously, we developed a protocol to generate cDC2s from cord blood (CB)-derived stem cells via a CD115-expressing precursor. Here, we performed index sorting and single-cell RNA-sequencing to define the heterogeneity of in vitro developed DC precursors and identified CD14+CD115+ expressing cells that develop into CD1c++DCs and the remainder cells brought about CD123+DCs, as well as assessed their potency. The maturation status and T-cell activation potential were assessed using flow cytometry. CD123+DCs were specifically prone to take up antigens but only modestly activated T-cells. In contrast, CD1c++ are highly mature and specialized in both naïve as well as antigen-experienced T-cell activation. These findings show in vitro functional diversity between cord blood stem cell-derived CD123+DC and CD1c++DCs and may advance the efficiency of DC-based vaccines.

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