scholarly journals NanoString nCounter® Approach in Breast Cancer: A Comparative Analysis with Quantitative Real-Time Polymerase Chain Reaction, In Situ Hybridization, and Immunohistochemistry

2017 ◽  
Vol 20 (3) ◽  
pp. 286 ◽  
Author(s):  
Jiyeon Hyeon ◽  
Soo Youn Cho ◽  
Min Eui Hong ◽  
So Young Kang ◽  
Ingu Do ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Comparative Analysis ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Severe Human Metapneumovirus Infection by Real-Time Polymerase Chain Reaction and Histopathological Assessment

2005 ◽  
Vol 192 (6) ◽  
pp. 1052-1060 ◽  
Author(s):  
Kaharu C. Sumino ◽  
Eugene Agapov ◽  
Richard A. Pierce ◽  
Elbert P. Trulock ◽  
John D. Pfeifer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Respiratory Tract ◽  
Real Time ◽  
Human Metapneumovirus ◽  
Chain Reaction ◽  
Hmpv Infection ◽  
Polymerase Chain ◽  
The Impact

Abstract BackgroundInfections with common respiratory tract viruses can cause high mortality, especially in immunocompromised hosts, but the impact of human metapneumovirus (hMPV) in this setting was previously unknown MethodsWe evaluated consecutive bronchoalveolar lavage and bronchial wash fluid samples from 688 patients—72% were immunocompromised and were predominantly lung transplant recipients—for hMPV by use of quantitative real-time polymerase chain reaction (PCR), and positive results were correlated with clinical outcome and results of viral cultures, in situ hybridization, and lung histopathological assessment ResultsSix cases of hMPV infection were identified, and they had a similar frequency and occurred in a similar age range as other paramyxoviral infections. Four of 6 infections occurred in immunocompromised patients. Infection was confirmed by in situ hybridization for the viral nucleocapsid gene. Histopathological assessment of lung tissue samples showed acute and organizing injury, and smudge cell formation was distinct from findings in infections with other paramyxoviruses. Each patient with high titers of hMPV exhibited a complicated clinical course requiring prolonged hospitalization ConclusionsOur results provide in situ evidence of hMPV infection in humans and suggest that hMPV is a cause of clinically severe lower respiratory tract infection that can be detected during bronchoscopy by use of real-time PCR and routine histopathological assessment

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