scholarly journals Molecular effects of sodium hyaluronate on the healing of avian supracoracoid tendon tear: According to in situ hybridization and real-time polymerase chain reaction

2007 ◽  
Vol 25 (2) ◽  
pp. 173-184 ◽  
Author(s):  
Eiji Shimpuku ◽  
Kazutoshi Hamada ◽  
Akiyoshi Handa ◽  
Kunihiko Kobayashi ◽  
Tomotaka Nakajima ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Real Time ◽  
Sodium Hyaluronate ◽  
Chain Reaction ◽  
Tendon Tear ◽  
Molecular Effects ◽  
Polymerase Chain

scholarly journals Detection of Severe Human Metapneumovirus Infection by Real-Time Polymerase Chain Reaction and Histopathological Assessment

2005 ◽  
Vol 192 (6) ◽  
pp. 1052-1060 ◽  
Author(s):  
Kaharu C. Sumino ◽  
Eugene Agapov ◽  
Richard A. Pierce ◽  
Elbert P. Trulock ◽  
John D. Pfeifer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Respiratory Tract ◽  
Real Time ◽  
Human Metapneumovirus ◽  
Chain Reaction ◽  
Hmpv Infection ◽  
Polymerase Chain ◽  
The Impact

Abstract BackgroundInfections with common respiratory tract viruses can cause high mortality, especially in immunocompromised hosts, but the impact of human metapneumovirus (hMPV) in this setting was previously unknown MethodsWe evaluated consecutive bronchoalveolar lavage and bronchial wash fluid samples from 688 patients—72% were immunocompromised and were predominantly lung transplant recipients—for hMPV by use of quantitative real-time polymerase chain reaction (PCR), and positive results were correlated with clinical outcome and results of viral cultures, in situ hybridization, and lung histopathological assessment ResultsSix cases of hMPV infection were identified, and they had a similar frequency and occurred in a similar age range as other paramyxoviral infections. Four of 6 infections occurred in immunocompromised patients. Infection was confirmed by in situ hybridization for the viral nucleocapsid gene. Histopathological assessment of lung tissue samples showed acute and organizing injury, and smudge cell formation was distinct from findings in infections with other paramyxoviruses. Each patient with high titers of hMPV exhibited a complicated clinical course requiring prolonged hospitalization ConclusionsOur results provide in situ evidence of hMPV infection in humans and suggest that hMPV is a cause of clinically severe lower respiratory tract infection that can be detected during bronchoscopy by use of real-time PCR and routine histopathological assessment

miR-21 Is Overexpressed in Hydatidiform Mole Tissues and Promotes Proliferation, Migration, and Invasion in Choriocarcinoma Cells

2017 ◽  
Vol 27 (2) ◽  
pp. 364-374 ◽  
Author(s):  
Ya-Xin Wang ◽  
Jiu-Ru Zhao ◽  
Yue-Ying Xu ◽  
Wei-Bin Wu ◽  
Hui-Juan Zhang
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Real Time ◽  
Hydatidiform Mole ◽  
Flow Cytometric Analysis ◽  
First Trimester ◽  
Migration And Invasion ◽  
Chain Reaction ◽  
Polymerase Chain

ObjectiveThe aims of this study were to make clear whether miR-21 was dysregulated in hydatidiform mole (HM) tissues and choriocarcinoma (CCA) cells, to elucidate whether aberrant miR-21 expression would affect the function of CCA cells, and to find out whether there was a relationship between miR-21 andAKT,PDCD4, andPTENin CCA cells.MethodsFresh and formalin-fixed, paraffin-embedded trophoblastic tissues (normal first trimester placentas and HMs) were retrieved from the biobank in the International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University. Choriocarcinoma JAR and JEG-3 cells were cultured. Expression of miR-21 in trophoblast cells and tissues was examined by quantitative real-time polymerase chain reaction. Location and distribution of miR-21 in trophoblast tissues were determinated by in situ hybridization and fluorescent in situ hybridization. The effect of miR-21 on JAR and JEG-3 cells was tested by miR-21 mimics and inhibitor transfection, followed by cell viability assay, flow cytometric analysis, and Transwell analysis. Interaction between miR-21 and its target genes in CCA cells was verified by quantitative real-time polymerase chain reaction, Western blot, and luciferase report system.ResultsWe originally found miR-21 was markedly upregulated in HM tissues compared with normal first trimester placentas. The expression of miR-21 was exclusively confined in trophoblastic layers. Furthermore, we discovered miR-21 was significantly increased in JAR and JEG-3 cells compared with normal primary human trophoblastic cells. Moreover, we demonstrated miR-21 could promote proliferation, migration, and invasion of CCA cells. We furthermore proved miR-21 negatively regulated PDCD4 and PTEN in CCA cells and targeted toPDCD43′UTR directly. In addition, we confirmed that miR-21 could activate Akt pathway by phosphorylating Akt at Ser 473.ConclusionsOur results suggested miR-21 was responsible for aggressive phenotype of gestational trophoblastic disease and had the potential diagnostic and therapeutic values for gestational trophoblastic neoplasm.

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