SS: High Pressure Gas-Liquid Separation: High-pressure droplet-deposition: from experiments to closure laws

2010 ◽  
Author(s):  
Pablo Matas Dupuy ◽  
Hugo Atle Jakobsen ◽  
Maria Fernandino ◽  
Hallvard Svendsen ◽  
Ansor Gaebler
Keyword(s):  
High Pressure ◽  
Droplet Deposition ◽  
Liquid Separation ◽  
Closure Laws

Scrubber Separation--Droplet Entrainment in High-Pressure Gas/Liquid Separation

2010 ◽  
Vol 5 (04) ◽  
pp. 183-191
Author(s):  
Luciano E. Patruno ◽  
Jorge Marchetti ◽  
Carlos Dorao ◽  
Hugo Jakobsen ◽  
Hallvard Svendsen ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Separation ◽  
Droplet Entrainment

High Pressure Gas-Liquid Separation: Diffusion Coefficient, the Silent Coalescence Inhibitor

2010 ◽  
Author(s):  
P.M. Dupuy ◽  
M. Fernandino ◽  
H.A. Jakobsen ◽  
H. Svendsen
Keyword(s):  
High Pressure ◽  
Diffusion Coefficient ◽  
Liquid Separation

SS: Special Session Title: High Pressure Gas-Liquid Separation. Droplet size measuring technique and application

2010 ◽  
Author(s):  
Jorge Mario Marchetti ◽  
Luciano Emanuel Patruno ◽  
Ansor Gaebler ◽  
A.G. Winterthur ◽  
Hallvard Svendsen
Keyword(s):  
High Pressure ◽  
Droplet Size ◽  
Measuring Technique ◽  
Special Session ◽  
Liquid Separation

SS: High Pressure Gas Liquid Separation: Scrubber separation - Droplet Entrainment in High Pressure Gas Liquid Separation

2010 ◽  
Author(s):  
Luciano Emanuel Patruno ◽  
Jorge Mario Marchetti ◽  
Carlos Alberto Dorao ◽  
Hugo Atle Jakobsen ◽  
Hallvard Svendsen ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Separation ◽  
Droplet Entrainment

High-pressure freezing of cells in suspension for low-voltage scanning electron microscopy (LVSEM)

1991 ◽  
Vol 49 ◽  
pp. 64-65
Author(s):  
Marek Malecki ◽  
James Pawley ◽  
Hans Ris
Keyword(s):  
Electron Microscopy ◽  
High Pressure ◽  
Low Voltage ◽  
Culture Media ◽  
Cell Structure ◽  
Freeze Substitution ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Cell Culture Media ◽  
Voltage Scanning

The ultrastructure of cells suspended in physiological fluids or cell culture media can only be studied if the living processes are stopped while the cells remain in suspension. Attachment of living cells to carrier surfaces to facilitate further processing for electron microscopy produces a rapid reorganization of cell structure eradicating most traces of the structures present when the cells were in suspension. The structure of cells in suspension can be immobilized by either chemical fixation or, much faster, by rapid freezing (cryo-immobilization). The fixation speed is particularly important in studies of cell surface reorganization over time. High pressure freezing provides conditions where specimens up to 500μm thick can be frozen in milliseconds without ice crystal damage. This volume is sufficient for cells to remain in suspension until frozen. However, special procedures are needed to assure that the unattached cells are not lost during subsequent processing for LVSEM or HVEM using freeze-substitution or freeze drying. We recently developed such a procedure.

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