Ticks on small mammals at two localities in southern Saskatchewan, Canada and the detection ofRickettsia peacockii(Rickettsiaceae) inDermacentor andersoni(Acari: Ixodidae) nymphs

2015 ◽  
Vol 148 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Shaun J. Dergousoff ◽  
Neil B. Chilton
Keyword(s):  
Polymerase Chain Reaction ◽  
Small Mammals ◽  
Small Mammal ◽  
Tick Species ◽  
Dermacentor Variabilis ◽  
Microtus Pennsylvanicus ◽  
Chain Reaction ◽  
Ictidomys Tridecemlineatus ◽  
Polymerase Chain ◽  
Provincial Park

AbstractSeventeenMyodes gapperi(Vigors) (Rodentia: Cricetidae), 13Peromyscus maniculatus(Wagner) (Rodentia: Cricetidae), 12Microtus pennsylvanicus(Ord) (Rodentia: Cricetidae), fourZapus princepsAllen (Rodentia: Cricetidae), threeIctidomys tridecemlineatus(Mitchill) (Rodentia: Sciuridae), and eight shrews (Soricomorpha: Soricidae) collected at Blackstrap Lake (BL), and 48P. maniculatus, 15Z. princeps,15M. pennsylvanicus, and oneSorex monticolusMerriam (Soricomorpha: Soricidae) collected at Saskatchewan Landing Provincial Park (SLPP), in southern Saskatchewan, Canada were examined for ticks. Although no adult ticks were detected on small mammals at either locality,Dermacentor variabilis(Say) (Acari: Ixodidae) larvae (n=144) and nymphs (n=7) were found on four species of small mammal at BL. At SLPP, bothD. variabilislarvae (n=71) and nymphs (n=6), andDermacentor andersoniStiles (Acari: Ixodidae) nymphs (n=9) were collected from small mammals. Both tick species were present onP. maniculatusandM. pennsylvanicusat SLPP, indicating an overlap in their host range and, hence, the potential for transmission of microorganisms between tick species at sites where they coexist. However, the results of polymerase chain reaction assays used to detect bacteria of the genusRickettsiada Rocha-Lima (Rickettsiaceae) in ticks, revealed thatR. peacockiiNiebylskiet al. only occurred in nymphs ofD. andersoni, whereas noRickettsiawere present in the larvae and nymphs ofD. variabilis.

scholarly journals Classification of Tick Species and Detection of Tick-borne Pathogens in Yanbian, China

2020 ◽  
Author(s):  
Jixu Li ◽  
Shuang Zhang ◽  
Wanfeng Liang ◽  
Shaowei Zhao ◽  
Hao Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
North Korea ◽  
Tick Species ◽  
Spotted Fever ◽  
Ixodes Persulcatus ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Severe Fever

Abstract BackgroundYanbian is located at the junction between China, Russia, and North Korea. We aimed to determine the species distribution and pathogens carried by ticks in Yanbian.MethodsA total of 2673 unattached ticks were collected from eight counties and cities in Yanbian and classified morphologically. Candidatus Rickettsia tarasevichiae (CRT), spotted fever group Rickettsia (SFGR), severe fever thrombocytopenia syndrome virus (SFTSV), Theileria, and other pathogens were detected using polymerase chain reaction (PCR) and real-time quantitative polymerase chain reaction followed by phylogenetic and genotypic analyses.ResultsAccording to the morphological classification, the main tick species in Yanbian were Haemaphysalis longicornis, Ixodes persulcatus, Dermacentor silvarum, Haemaphysalis japonica, and Haemaphysalis concinna. Candidatus Rickettsia tarasevichiae, spotted fever group Rickettsia, severe fever thrombocytopenia syndrome virus, and Theileria orientalis were detected in H. longicornis, Candidatus Rickettsia tarasevichiae, spotted fever group Rickettsia, and severe fever thrombocytopenia syndrome virus were detected in I. persulcatus, H. japonica, and D. silvarum, but only severe fever thrombocytopenia syndrome virus was detected in H. concinna. Mixed infection with Candidatus Rickettsia tarasevichiae and severe fever thrombocytopenia syndrome virus was found in I. persulcatus and H. japonica. The gene sequences of all tested pathogens exhibited 95.7%–100% homology with sequences registered in GenBank. Phylogenetic analysis showed that different spotted fever group Rickettsia and severe fever thrombocytopenia syndrome virus genotypes were closely related to the Korean strains. We provide the first evidence for the presence of the spotted fever group Rickettsia genotypes of Candidatus Rickettsia longicornii, ompA, ompB, sca4, and rrs, in Haemaphysalis longicornis in Yanbian. ConclusionsThese results provide epidemiological data to support the prevention and control of ticks and tick-borne diseases in the border areas of China, North Korea, and Russia.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

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