scholarly journals Differentiation of Medically Important Euro-Asian Tick Species Ixodes ricinus, Ixodes persulcatus, Ixodes hexagonus, and Dermacentor reticulatus by Polymerase Chain Reaction

2011 ◽  
Vol 11 (7) ◽  
pp. 899-905 ◽  
Author(s):  
Leonid Rumer ◽  
Olga Sheshukova ◽  
Hans Dautel ◽  
Oliver Donoso Mantke ◽  
Matthias Niedrig
Keyword(s):  
Polymerase Chain Reaction ◽  
Ixodes Ricinus ◽  
Tick Species ◽  
Dermacentor Reticulatus ◽  
Ixodes Persulcatus ◽  
Chain Reaction ◽  
Ixodes Hexagonus ◽  
Polymerase Chain

scholarly journals Classification of Tick Species and Detection of Tick-borne Pathogens in Yanbian, China

2020 ◽  
Author(s):  
Jixu Li ◽  
Shuang Zhang ◽  
Wanfeng Liang ◽  
Shaowei Zhao ◽  
Hao Wang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
North Korea ◽  
Tick Species ◽  
Spotted Fever ◽  
Ixodes Persulcatus ◽  
Spotted Fever Group ◽  
Haemaphysalis Longicornis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Severe Fever

Abstract BackgroundYanbian is located at the junction between China, Russia, and North Korea. We aimed to determine the species distribution and pathogens carried by ticks in Yanbian.MethodsA total of 2673 unattached ticks were collected from eight counties and cities in Yanbian and classified morphologically. Candidatus Rickettsia tarasevichiae (CRT), spotted fever group Rickettsia (SFGR), severe fever thrombocytopenia syndrome virus (SFTSV), Theileria, and other pathogens were detected using polymerase chain reaction (PCR) and real-time quantitative polymerase chain reaction followed by phylogenetic and genotypic analyses.ResultsAccording to the morphological classification, the main tick species in Yanbian were Haemaphysalis longicornis, Ixodes persulcatus, Dermacentor silvarum, Haemaphysalis japonica, and Haemaphysalis concinna. Candidatus Rickettsia tarasevichiae, spotted fever group Rickettsia, severe fever thrombocytopenia syndrome virus, and Theileria orientalis were detected in H. longicornis, Candidatus Rickettsia tarasevichiae, spotted fever group Rickettsia, and severe fever thrombocytopenia syndrome virus were detected in I. persulcatus, H. japonica, and D. silvarum, but only severe fever thrombocytopenia syndrome virus was detected in H. concinna. Mixed infection with Candidatus Rickettsia tarasevichiae and severe fever thrombocytopenia syndrome virus was found in I. persulcatus and H. japonica. The gene sequences of all tested pathogens exhibited 95.7%–100% homology with sequences registered in GenBank. Phylogenetic analysis showed that different spotted fever group Rickettsia and severe fever thrombocytopenia syndrome virus genotypes were closely related to the Korean strains. We provide the first evidence for the presence of the spotted fever group Rickettsia genotypes of Candidatus Rickettsia longicornii, ompA, ompB, sca4, and rrs, in Haemaphysalis longicornis in Yanbian. ConclusionsThese results provide epidemiological data to support the prevention and control of ticks and tick-borne diseases in the border areas of China, North Korea, and Russia.

scholarly journals Tick-borne pathogens in ticks collected from dogs, Latvia, 2011–2016

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Agne Namina ◽  
Valentina Capligina ◽  
Maija Seleznova ◽  
Rudolfs Krumins ◽  
Darja Aleinikova ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Tick Species ◽  
Environmental Changes ◽  
Dermacentor Reticulatus ◽  
Ixodes Persulcatus ◽  
Borrelia Miyamotoi ◽  
Emerging Pathogens ◽  
Additional Risk ◽  
Risk Of Exposure ◽  
The Difference

Abstract Background Different tick species are able to transmit different pathogens, and tick-borne diseases are of substantial concern worldwide for both humans and animals. Environmental changes and changes in the range of tick species, including Dermacentor reticulatus in Europe, can affect the spread of zoonotic pathogens. The aim of this study was to investigate the prevalence of the tick-borne pathogens in ticks removed from dogs in Latvia, and to explore possible changes between years 2011 and 2016. Results In 2011, only Ixodes ticks (221 Ixodes ricinus and 22 Ixodes persulcatus) were collected from dogs, while in 2016 tick samples belonged to Ixodes ricinus (360), Ixodes persulcatus (2) and Dermacentor reticulatus (27) species. In total, 35.8 and 40.0% of adult ticks were pathogen-positive in 2011 and 2016, respectively; the difference was not statistically significant (P > 0.05). The molecular analysis indicated the presence of 13 tick-borne microorganisms; the most prevalent pathogen was Rickettsia, followed by Borrelia burgdorferi sensu lato group spirochetes, Anaplasma phagocytophilum and Babesia species. Borrelia miyamotoi was also present. A co-infection with two and three tick-borne pathogens was detected in 7.9 and 7.4% of Ixodes ricinus and Dermacentor reticulatus, respectively. The results of this study confirmed that the spread of novel vectors could bring additional risk of exposure to novel emerging pathogens to pets and their owners, as both Babesia canis and Rickettsia raoultii were shown to be highly associated with Dermacentor reticulatus ticks in Latvia. Conclusions This study demonstrates the potential danger from the inadvertent introduction of novel disease pathogens and vectors. Awareness of co-infections and Dermacentor reticulatus-related pathogens needs to be increased.

Ticks on small mammals at two localities in southern Saskatchewan, Canada and the detection ofRickettsia peacockii(Rickettsiaceae) inDermacentor andersoni(Acari: Ixodidae) nymphs

2015 ◽  
Vol 148 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Shaun J. Dergousoff ◽  
Neil B. Chilton
Keyword(s):  
Polymerase Chain Reaction ◽  
Small Mammals ◽  
Small Mammal ◽  
Tick Species ◽  
Dermacentor Variabilis ◽  
Microtus Pennsylvanicus ◽  
Chain Reaction ◽  
Ictidomys Tridecemlineatus ◽  
Polymerase Chain ◽  
Provincial Park

AbstractSeventeenMyodes gapperi(Vigors) (Rodentia: Cricetidae), 13Peromyscus maniculatus(Wagner) (Rodentia: Cricetidae), 12Microtus pennsylvanicus(Ord) (Rodentia: Cricetidae), fourZapus princepsAllen (Rodentia: Cricetidae), threeIctidomys tridecemlineatus(Mitchill) (Rodentia: Sciuridae), and eight shrews (Soricomorpha: Soricidae) collected at Blackstrap Lake (BL), and 48P. maniculatus, 15Z. princeps,15M. pennsylvanicus, and oneSorex monticolusMerriam (Soricomorpha: Soricidae) collected at Saskatchewan Landing Provincial Park (SLPP), in southern Saskatchewan, Canada were examined for ticks. Although no adult ticks were detected on small mammals at either locality,Dermacentor variabilis(Say) (Acari: Ixodidae) larvae (n=144) and nymphs (n=7) were found on four species of small mammal at BL. At SLPP, bothD. variabilislarvae (n=71) and nymphs (n=6), andDermacentor andersoniStiles (Acari: Ixodidae) nymphs (n=9) were collected from small mammals. Both tick species were present onP. maniculatusandM. pennsylvanicusat SLPP, indicating an overlap in their host range and, hence, the potential for transmission of microorganisms between tick species at sites where they coexist. However, the results of polymerase chain reaction assays used to detect bacteria of the genusRickettsiada Rocha-Lima (Rickettsiaceae) in ticks, revealed thatR. peacockiiNiebylskiet al. only occurred in nymphs ofD. andersoni, whereas noRickettsiawere present in the larvae and nymphs ofD. variabilis.

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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