Identification of chemosensory gene families in Rhyzopertha dominica (Coleoptera: Bostrichidae)

2015 ◽  
Vol 148 (1) ◽  
pp. 8-21 ◽  
Author(s):  
Mory Mandiana Diakite ◽  
Juan Wang ◽  
Suliman Ali ◽  
Man-Qun Wang
Keyword(s):  
Binding Proteins ◽  
Acyrthosiphon Pisum ◽  
Gene Families ◽  
Rhyzopertha Dominica ◽  
Base Pairs ◽  
Chemosensory Gene ◽  
Long Reads ◽  
Mean Size ◽  
Receptor Neurons ◽  
Odorant Binding

AbstractChemoreception is a key process for insects. Odorant messages diffuse through the air and are translated into physiological signals by chemosensory receptor neurons in sensilla that are mainly located on insect antennae. We sequenced the antenna transcriptome of Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), which is a serious pest of stored grains throughout regions with warm climates, and performed transcriptome analysis on R. dominica antennae. We obtained 57 million 90-base pair-long reads that we assembled into 37 877 unigenes with a mean size of 1007 base pairs. Predicted protein sequences were matched with Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) (79.1%), Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae) (1.7%), Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae) (1.3%), Acyrthosiphon pisum Harris (Hemiptera: Aphididae) (1.2%), and other (16.7%) homologues. In chemosensory gene families, we identified transcripts that encoded the following putative genes: 12 odorant-binding proteins (OBPs), four pheromone-binding proteins (PBPs), eight chemosensory proteins (CSPs), five sensory neuron membrane proteins (SNMPs), six odorant receptors, and eight ionotropic receptors. The diversity of the predicted OBPs, PBPs, and CSPs are also discussed. These findings will advance our understanding of olfaction process by this pest.

scholarly journals Contribution of odorant binding proteins to olfactory detection of (Z)-11-hexadecenal in Helicoverpa armigera

2020 ◽  
Author(s):  
Hao Guo ◽  
Ping-Ping Guo ◽  
Ya-Lan Sun ◽  
Ling-Qiao Huang ◽  
Chen-Zhu Wang
Keyword(s):  
Helicoverpa Armigera ◽  
Binding Proteins ◽  
Supporting Cells ◽  
Odorant Binding Proteins ◽  
Sensilla Trichodea ◽  
Helicoverpa Armígera ◽  
Olfactory Detection ◽  
Receptor Neurons ◽  
Pheromone Binding Proteins ◽  
Odorant Binding

AbstractHelicoverpa armigera utilizes (Z)-11-hexadecenal (Z11-16:Ald) as its major sex pheromone component. Three pheromone binding proteins (PBPs) and two general odorant binding proteins (GOBPs) are abundantly expressed in male antennae of H. armigera. However, their precise roles in the olfactory detection of Z11-16:Ald remain enigmatic. To answer this question, we first synthesized the antibody against HarmOR13, a pheromone receptor (PR) primarily responding to Z11-16:Ald and mapped the local associations between PBPs / GOBPs and HarmOR13. Immunostaining showed that HarmPBPs and HarmGOBPs were localized in the supporting cells of sensilla trichodea and sensilla basiconica respectively. In particular, HarmPBP1 and HarmPBP2 were colocalized in the cells surrounding the olfactory receptor neurons (ORNs) expressing HarmOR13. Next, using two noninterfering binary expression tools, we heterologously expressed HarmPBP1, HarmPBP2 and HarmOR13 in Drosophila T1 sensilla to validate the functional interplay between PBPs and HarmOR13. We found that the addition of HarmPBP1 or HarmPBP2 significantly increased the sensitivity of HarmOR13 to Z11-16:Ald. However, the presence of either HarmPBP1 or HarmPBP2 was ineffective to change the tuning breadth of HarmOR13. Taken together, our results support the idea that PBPs are contributors to the peripheral olfactory sensitivity but do not affect the selectivity. Lastly, we discovered that HarmOR13 and the Drosophila OR67d employed a similar coding mechanism to detect pheromones, suggesting that pheromone detection across different insect orders appears to co-opt a conserved molecular principle to recognize pheromone ligands.

scholarly journals Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae)

Insects ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 175 ◽  
Author(s):  
Ji-Wei Xu ◽  
Xiu-Yun Zhu ◽  
Qiu-Jie Chao ◽  
Yong-Jie Zhang ◽  
Yu-Xia Yang ◽  
...  
Keyword(s):  
Insect Behavior ◽  
Gene Families ◽  
Tissue Expression ◽  
Specific Expression ◽  
Female Head ◽  
Chemosensory Gene ◽  
Chemosensory Genes ◽  
Male Head ◽  
Chemosensory Systems ◽  
Odorant Binding

Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.

scholarly journals Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

2021 ◽  
Vol 9 ◽  
Author(s):  
Zheng-Quan Wang ◽  
Chun Wu ◽  
Gen-Ceng Li ◽  
Shu-Mei Nuo ◽  
Ning-Na Yin ◽  
...  
Keyword(s):  
Sex Pheromones ◽  
Gene Families ◽  
Odorant Receptors ◽  
Neuron Membrane ◽  
Odorant Binding Proteins ◽  
Chemosensory Gene ◽  
Chemosensory Genes ◽  
Lepidoptera Pyralidae ◽  
Odorant Binding

In Lepidoptera, RNA sequencing has become a useful tool in identifying chemosensory genes from antennal transcriptomes, but little attention is paid to non-antennal tissues. Though the antennae are primarily responsible for olfaction, studies have found that a certain number of chemosensory genes are exclusively or highly expressed in the non-antennal tissues, such as proboscises, legs and abdomens. In this study, we report a global transcriptome of 16 tissues from Dioryctria abietella, including chemosensory and non-chemosensory tissues. Through Illumina sequencing, totally 952,658,466 clean reads were generated, summing to 142.90 gigabases of data. Based on the transcriptome, 235 chemosensory-related genes were identified, comprising 42 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs), 75 odorant receptors (ORs), 62 gustatory receptors (GRs), 30 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). Compared to a previous study in this species, 140 novel genes were found. A transcriptome-wide analysis combined with PCR results revealed that except for GRs, the majority of other five chemosensory gene families in Lepidoptera were expressed in the antennae, including 160 chemosensory genes in D. abietella. Using phylogenetic and expression profiling analyses, members of the six chemosensory gene repertoires were characterized, in which 11 DabiORs were candidates for detecting female sex pheromones in D. abietella, and DabiOR23 may be involved in the sensing of plant-derived phenylacetaldehyde. Intriguingly, more than half of the genes were detected in the proboscises, and one fourth of the genes were found to have the expression in the legs. Our study not only greatly extends and improves the description of chemosensory genes in D. abietella, but also identifies potential molecular targets involved in olfaction, gustation and non-chemosensory functions for control of this pest.

scholarly journals A Detailed Spatial Expression Analysis of Wing Phenotypes Reveals Novel Patterns of Odorant Binding Proteins in the Soybean Aphid, Aphis glycines

2021 ◽  
Vol 12 ◽  
Author(s):  
Ling Wang ◽  
Hang Yin ◽  
Zhiguo Zhu ◽  
Shuai Yang ◽  
Jia Fan
Keyword(s):  
Binding Proteins ◽  
Expression Patterns ◽  
Gene Families ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Rapid Expansion ◽  
Odorant Binding Proteins ◽  
Spatial Expression ◽  
Wide Range ◽  
Odorant Binding

The wide range of insect niches has led to a rapid expansion of chemosensory gene families as well as their relatively independent evolution and a high variation. Previous studies have revealed some functions for odorant-binding proteins (OBPs) in processes beyond olfaction, such as gustation and reproduction. In this study, a comparative transcriptomic analysis strategy was applied for the soybean aphid, Aphis glycines, focusing on various functional tissues and organs of winged aphids, including the antenna, head, leg, wing, thorax, cauda, and cornicle. Detailed spatial OBP expression patterns in winged and wingless parthenogenetic aphids were detected by RT-qPCR. Twelve OBPs were identified, and three new OBPs in A. glycines are first reported. All OBPs showed comparatively higher expression in sensory organs and tissues, such as the antenna, head, or leg. Additionally, we found some novel expression patterns for aphid OBPs (Beckendorf et al., 2008). Five OBPs exhibited high-expression levels in the cauda and four in the cornicle (Biasio et al., 2015). Three genes (OBP2/3/15) were highly expressed in the wing (Calvello et al., 2003). Two (OBP3/15) were significantly more highly expressed in the wingless thorax than in the winged thorax with the wings removed, and these transcripts were significantly enriched in the removed wings. More details regarding OBP spatial expression were revealed under our strategy. These findings supported the existence of carrier transport functions other than for foreign chemicals and therefore broader ligand ranges of aphid OBPs. It is important for understanding how insect OBPs function in chemical perception as well as their other potential physiological functions.

scholarly journals Comparative Genomics Provide Insights Into Function and Evolution of Odorant Binding Proteins in Cydia pomonella

2021 ◽  
Vol 12 ◽  
Author(s):  
Cong Huang ◽  
Xue Zhang ◽  
Dongfeng He ◽  
Qiang Wu ◽  
Rui Tang ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Cydia Pomonella ◽  
Purifying Selection ◽  
Codling Moth ◽  
Economic Losses ◽  
Odorant Binding Proteins ◽  
Host Seeking ◽  
Receptor Neurons ◽  
Gains And Losses ◽  
Odorant Binding

Insect olfaction is vital for foraging, mating, host-seeking, and avoidance of predators/pathogens. In insects, odorant binding proteins (OBPs) are involved in transporting hydrophobic odor molecules from the external environment to receptor neurons. The codling moth, Cydia pomonella, one of the most destructive insect fruit pests, causes enormous economic losses. However, little is known about the number, variety, gains and losses, and evolution of OBP genes in C. pomonella. Here we report the identification of 40 OBPs in C. pomonella, most (75%) of which are classic OBPs, using genomic and transcriptomic analyses. Two OBP genes were lost in C. pomonella relative to possible distant ancestor in Lepidoptera lineage based on an analysis of gene gains and losses. The phylogenetic tree and chromosome location showed that the expansion of OBP genes mainly resulted from tandem duplications, as the CpomGOBP2 gene was duplicated twice along with loss of CpomPBPB. Two positive selection sites of the CpomGOBP1 gene were identified while other OBP genes evolved under purifying selection. Our results provide fundamental knowledge of OBP genes allowing further study of their function in C. pomonella.

scholarly journals Antennal transcriptome analysis and identification of candidate chemosensory genes of the harlequin ladybird beetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)

2021 ◽  
Author(s):  
Gabriele Rondoni ◽  
Alessandro Roman ◽  
Camille Meslin ◽  
Nicolas Montagné ◽  
Eric Conti ◽  
...  
Keyword(s):  
Biological Control ◽  
Binding Proteins ◽  
Harmonia Axyridis ◽  
Gene Repertoire ◽  
Odorant Binding Proteins ◽  
Harlequin Ladybird ◽  
Chemosensory Gene ◽  
Chemosensory Genes ◽  
Males And Females ◽  
Odorant Binding

AbstractIn predatory ladybirds (Coleoptera: Coccinellidae), antennae are important for chemosensory reception used during food and mate location, and for finding a suitable oviposition habitat. Based on NextSeq 550 Illumina sequencing, we assembled the antennal transcriptome of mated Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) males and females and described the first chemosensory gene repertoire expressed in this species. We annotated candidate chemosensory sequences encoding 26 odorant receptors (including the coreceptor, Orco), 17 gustatory receptors, 27 ionotropic receptors, 31 odorant-binding proteins, 12 chemosensory proteins and 4 sensory neuron membrane proteins. Maximum-likelihood phylogenetic analyses allowed to assign candidate H. axyridis chemosensory genes to previously described groups in each of these families. Differential expression analysis between males and females revealed low variability between sexes, possibly reflecting the known absence of relevant sexual dimorphism in the structure of the antennae and in the distribution and abundance of the sensilla. However, we revealed significant differences in expression of three chemosensory genes, namely 2 male-biased odorant-binding proteins and 1 male-biased odorant receptor, suggesting their possible involvement in pheromone detection. Our data pave the way for improving the understanding of the molecular basis of chemosensory reception in Coccinellidae.SummaryThe predatory harlequin ladybird Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) has been widely released for classical and augmentative biological control programs of insect herbivores and is now distributed worldwide. Because of its invasive behavior and the threat it can pose to local biodiversity, this ladybird has been adopted as a model species for invasive biocontrol predators. A huge existing literature is available on this species. However, little is known about the mechanisms underlying H. axyridis smell and taste, even though these senses are important in this ladybird for courtship, mating and for locating suitable habitats for feeding and oviposition. Here we describe the first chemosensory gene repertoire that is expressed in the antennae of male and female H. axyridis. Our findings would likely represent the basis for future functional studies aiming at increasing the efficacy of H. axyridis in biological control or at reducing its populations in those areas where the ladybird has become a matter of concern due to its invasiveness.

scholarly journals Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Heliyon ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. e06427
Author(s):  
Haixia Zhan ◽  
Du Li ◽  
Youssef Dewer ◽  
Changying Niu ◽  
Fengqi Li ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Functional Characterization ◽  
Drosophila Suzukii ◽  
Odorant Binding Proteins ◽  
Odorant Binding

Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

2019 ◽  
Vol 27 (5) ◽  
pp. 1019-1030 ◽  
Author(s):  
Ling Wang ◽  
Ying‐Dong Bi ◽  
Ming Liu ◽  
Wei Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Expression Profiles ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Odorant Binding Proteins ◽  
Odorant Binding

scholarly journals Haplotype-resolved genome of diploid ginger (Zingiber officinale) and its unique gingerol biosynthetic pathway

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Hong-Lei Li ◽  
Lin Wu ◽  
Zhaoming Dong ◽  
Yusong Jiang ◽  
Sanjie Jiang ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Southwest China ◽  
Reference Genome ◽  
Zingiber Officinale ◽  
Gene Families ◽  
Chromosome Conformation ◽  
Long Reads ◽  
Transcription Factor Networks ◽  
Species Specific ◽  
Haplotype 1

AbstractGinger (Zingiber officinale), the type species of Zingiberaceae, is one of the most widespread medicinal plants and spices. Here, we report a high-quality, chromosome-scale reference genome of ginger ‘Zhugen’, a traditionally cultivated ginger in Southwest China used as a fresh vegetable, assembled from PacBio long reads, Illumina short reads, and high-throughput chromosome conformation capture (Hi-C) reads. The ginger genome was phased into two haplotypes, haplotype 1 (1.53 Gb with a contig N50 of 4.68 M) and haplotype 0 (1.51 Gb with a contig N50 of 5.28 M). Homologous ginger chromosomes maintained excellent gene pair collinearity. In 17,226 pairs of allelic genes, 11.9% exhibited differential expression between alleles. Based on the results of ginger genome sequencing, transcriptome analysis, and metabolomic analysis, we proposed a backbone biosynthetic pathway of gingerol analogs, which consists of 12 enzymatic gene families, PAL, C4H, 4CL, CST, C3’H, C3OMT, CCOMT, CSE, PKS, AOR, DHN, and DHT. These analyses also identified the likely transcription factor networks that regulate the synthesis of gingerol analogs. Overall, this study serves as an excellent resource for further research on ginger biology and breeding, lays a foundation for a better understanding of ginger evolution, and presents an intact biosynthetic pathway for species-specific gingerol biosynthesis.

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