The Morphology, Histology, and Fine Structure of the Gut of the Green Peach Aphid, Myzus persicae (Sulzer)

1964 ◽  
Vol 96 (1-2) ◽  
pp. 110-110
Author(s):  
A. R. Forbes
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Cross Section ◽  
Myzus Persicae ◽  
Green Peach Aphid ◽  
Posterior Wall ◽  
Anterior Wall ◽  
Pictorial Representation ◽  
Pump Chamber

A pictorial representation of the gut of the green peach aphid, Myzus persicae (Sulzer), is shown based on dissection, light microscopy, and electron microscopy.The sucking pump is crescentic in cross section with a thick, rigid, posterior wall and a thinner, flexible, anterior wall. Dilator muscles arise on the clypeus and are inserted along the midline of the anterior wall of the pump chamber. Morphologicael evidence of the sucking action of the pump is included.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

THE STYLETS OF THE GREEN PEACH APHID, MYZUS PERSICAE (HOMOPTERA: APHIDIDAE)

1969 ◽  
Vol 101 (1) ◽  
pp. 31-41 ◽  
Author(s):  
A. R. Forbes
Keyword(s):  
Fine Structure ◽  
Host Plant ◽  
Myzus Persicae ◽  
Green Peach Aphid ◽  
Two Stage ◽  
Whole Mounts

AbstractThe morphology and fine structure of the maxillary and mandibular stylets of the green peach aphid, Myzus persicae (Sulzer), are described from sections, whole mounts, and two-stage replicas. The mechanisms of penetration of the host plant are discussed.

The fine structure of the vertical lobe of Octopus brain

1970 ◽  
Vol 258 (827) ◽  
pp. 379-394 ◽  
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Synaptic Vesicles ◽  
Structural Organization ◽  
Amacrine Cells ◽  
Large Cell ◽  
Inhibitory Effect ◽  
Tactile System

Although much is known about the structural organization and connexions of the various lobes of the octopus brain from light microscopy, this is the first attempt at a detailed analysis of one of the lobes— the vertical lobe, with the electron microscope. The vertical lobe consists of five lobules. The median superior frontal (MSF) axons enter each lobule from the MSF lobe. The MSF axons contain both microtubules and neurofilaments. The varicosities of the MSF axons contain both agranular and dense-cored vesicles and synapse with trunks of the amacrine cells. These trunks run together in bundles termed amacrine tracts into the centres of the lobules. The amacrine trunks contain microtubules but no neurofilaments. The trunks contain large and small agranular synaptic vesicles and synapse with what are in all probability branches of the trunks of the large cells. These trunks contain microtubules but no neurofilaments. They run out through the bases of the lobules probably without forming synaptic contacts within the lobule. Fibres signalling ‘pain’ (nocifensor) enter the lobules from below. They can be recognized by their content of neurofilaments. Their terminals contain numerous very small synaptic vesicles and a few larger and dense-cored ones. These ‘pain’ fibres appear to synapse mostly with processes of the large cells. J. Z. Young has shown that the vertical lobe is especially concerned with the integrative action of the visual system, linked with the chemo-tactile system. Electron microscopy supports Young’s suggestion that the superior frontal and interconnected vertical lobe systems constitute a loop which could sustain a positive feed-back mechanism (MSF —> amacrine -> large cell -> lateral superior frontal -> MSF) while the ‘pain’ (nocifensor) input could exert a suppressor (inhibitory) effect on the loop by its action on the large cells.

scholarly journals Further Observations on the Fine Structure of Chrysochromulina Ericina Parke & Manton

1961 ◽  
Vol 41 (1) ◽  
pp. 145-155 ◽  
Author(s):  
I. Manton ◽  
G. F. Leedale
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Cell Surface ◽  
Light Microscopy ◽  
Flat Plate ◽  
Outer Layer ◽  
Living Cells ◽  
Layered Plates ◽  
Thin Sections ◽  
Micro Anatomy

C. ericina Parke & Manton has been re-investigated to add salient features of micro-anatomy from the electron microscopy of thin sections and also to add photographs of living cells taken with anoptral contrast light microscopy.The most important new observations concern the scales which are shown to be essentially two-layered plates in which the layers in the very large spined scales have become separated except at their edges, with the outer layer greatly hypertrophied to produce a hollow spine with a flared base closed at the bottom by a flat plate. The patterns of external marking on the two layers are very similar in both plate-scales and spines in this species and the orientation of both with respect to the cell surface has been demonstrated by a section of the scales in situ.

Fine Structure of Bacteria Grown in the Presence of Acriflavine

1968 ◽  
Vol 26 ◽  
pp. 70-71
Author(s):  
Edwin S. Boatman
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Yeast Extract ◽  
Bacterial Growth ◽  
Bacterial Species ◽  
The Other ◽  
Yeast Extract Medium ◽  
Peptone Water ◽  
Extract Medium

The effect of acridine derivatives on bacterial growth has been shown to be dependent upon the concentration used, pH, temperature, and the position of the amino substituents on the acridine molecule. These factors, in turn, affect the amount of acridine bound to cell constituents. Many bacterial species, when grown in media containing acridities, become filamentous, or pleomorphic, or growth may be entirely prevented. The fine-structure of two species of bacteria treated with acriflavine was investigated. Both were Gram-positive bacilli, one was a Corynebacterium, and the other an aerobic spore-bearing Bacillus.Organisms were incubated at 21°c in the presence of concentrations of acriflavine ranging from 0.25 ug/ml to 12 ug/ml in phosphate buffered peptone water yeast extract medium at pH 7.5. Viable counts were carried out and the amount of acriflavine bound, either reversibly or irreversibly, was estimated at 450 mu, using a DB spectrophotometer. Cultures were observed by light microscopy and, after four days growth, were processed for electron microscopy by fixation in veronal-acetate pH 6.1 buffered 0.8% Os O4 for one hour and embedding in Epon 812 resin.

Attachments of phasic and tonic abdominal extensor muscles in crayfish

1976 ◽  
Vol 54 (8) ◽  
pp. 1256-1269 ◽  
Author(s):  
S. S. Jahromi ◽  
H. L. Atwood
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Cross Section ◽  
Unit Area ◽  
Longitudinal Axis ◽  
Contractile Properties ◽  
Muscle Attachment ◽  
Tonic Muscle ◽  
Extensor Muscles ◽  
Extracellular Materials

The fine structure of attachments of phasic and tonic crayfish abdominal extensor muscles to the exoskeleton was studied by means of electron microscopy. Tonic muscles contract more slowly and exert more tension per unit area of cross section than phasic muscles. The muscle attachment regions show differences in structure which are correlated with the different contractile properties. In both muscles, microtubule-filled tendinous cells intervene between the end of the muscle and the exoskeleton; but in phasic muscles the tendinous cells are joined to the muscle by rather long extracellular microfibrils which in general run obliquely to the longitudinal axis of the muscle, whereas in tonic muscle, the tendinous cells and the muscle are joined together more directly by shorter microfibrils running parallel to the muscle's longitudinal axis, and also by desmosome-like structures. In addition, the extracellular materials in tonic muscles appear to be firmly attached to the Z lines of the muscle sarcomeres; such an association was not observed in phasic muscle.

Fine Structure of Pollen Walls in Lychnis Alba and the Presence of Silicon

1973 ◽  
Vol 31 ◽  
pp. 470-471
Author(s):  
Richard E. Crang
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Elemental Composition ◽  
Organic Compounds ◽  
Morphological Characterization ◽  
Flowering Plants ◽  
X Ray ◽  
Inorganic Composition

Acid resistant components of the pollen wall and tapetal membrane in flowering plants have long been known to be composed of sporopollenin—a highly resistant mixture of organic compounds. Recent light microscopy and cytochemical analyses have led to the recognition that additional organic compounds comprise a significant part of these anther structures. However, comparatively little consideration has been given to the inorganic composition of the pollen exine and the morphologically similar tapetal membrane. Electron microscopy allows the morphological characterization of components and, when coupled with energy dispersive x-ray analysis, offers the opportunity to characterize elemental composition.

Observations on the Fine Structure of Siliceous Microfossils from the Oamaru Diatomite (Upper Eocene), II. Archaeomonads.

1975 ◽  
Vol 33 ◽  
pp. 76-77
Author(s):  
John P. Jendrzejewski
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Life History ◽  
Fine Structure ◽  
Light Microscopy ◽  
Outer Layer ◽  
Upper Eocene ◽  
Resting Stages ◽  
History Of ◽  
Scanning Electron

Archaeomonads are a fossil group of marine siliceous cysts and are thought to represent resting stages in the life history of chrysophycean algae. The morphology of the cysts is generally simple with spherical, spheroidal, and elliptical outlines common. The cysts are characterized by the presence of an opening (pore) piercing the wall. A thickened structure (neck) often develops around the pore. The cyst usually is composed of one layer of silica although cysts with double and triple layers are common. The outer layer of the cyst may be smooth, or ornamented with spines, warts, pits, flanges or ridges of silica. Due to their small size (3-25 microns in diameter) and to the presence of minute ornamentation, identication of many forms solely with light microscopy is difficult. The application of scanning electron microscopy (SEM) together with light microscopy (LM) enables one to precisely determine the ornamentation and accurately define a species.

Fine structure of the microsymbiont of the actinorhizal root nodules of mountain mahogany (Cercocarpus ledifolius, family Rosaceae)

1989 ◽  
Vol 67 (1) ◽  
pp. 116-120 ◽  
Author(s):  
Susan M. Wood ◽  
William Newcomb ◽  
David Nelson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Root Nodules ◽  
Single Electron ◽  
Transmission Electron ◽  
Cercocarpus Ledifolius ◽  
Scanning Electron

Root nodules of Cercocarpus ledifolius Nutt. (mountain mahogany) were studied by light microscopy, scanning electron microscopy, and transmission electron microscopy to confirm the bacterial nature of the microsymbiont and to determine the morphology of the symbiotic vesicles. The microsymbiont is an actinomycete having two morphologies: septate hyphae (ca. 0.5 μm diam.) and ovoid- or elliptical-shaped nonseptate symbiotic vesicles (2.8 × 3.9 μm). Many of the symbiotic vesicles contain a single, electron-dense ovoid- or spherical-shaped structure, measuring 0.26 μm, whose function is unknown. The actinomycete is surrounded by a capsule that has electron-dense droplets in regions near hyphae. No spores or sporangia were observed in these nodules.

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