DIFFERENTIAL MORTALITY BETWEEN MALE AND FEMALE CHORISTONEURA OCCIDENTALIS (LEPIDOPTERA: TORTRICIDAE) LARVAE EXPOSED TO A BACULOVIRUS WITH OR WITHOUT OPTICAL BRIGHTENERS

1999 ◽  
Vol 131 (1) ◽  
pp. 65-70 ◽  
Author(s):  
S.Y. Li ◽  
I.S. Otvos
Keyword(s):  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Differential Mortality ◽  
Choristoneura Occidentalis ◽  
Male And Female ◽  
Optical Brighteners ◽  
Sublethal Doses ◽  
Males And Females ◽  
Nuclear Polyhedrosis ◽  
Tinopal Lpw

AbstractFifth-instar larval mortality was compared between male and female Choristoneura occidentalis Freeman exposed in the laboratory to sublethal doses of Choristoneura fumiferana (Clem.) multicapsid nuclear polyhedrosis virus (CfMNPV) with or without optical brighteners. More females than males died when the virus was used alone, but differences were not significant. When 1% brightener was added to CfMNPV suspension, differences in larval mortality between males and females were significant for three of the four brighteners tested. In addition, times at which 50% of the larvae died indicated that female larvae died 23 and 39% more quickly than male larvae, respectively, when brightener Blankophor HRS and Tinopal LPW were added to the virus, whereas at times at which 95% of the larvae died indicated that females died 33 and 54% faster than males. Alteration of sex ratio favoring male survival can play a significant role in the biological control of C. occidentalis by the virus.

Differences in activity enhancement of a baculovirus by an optical brightener between Choristoneura fumiferana and Choristoneura occidentalis (Lepidoptera: Tortricidae)

2001 ◽  
Vol 133 (3) ◽  
pp. 329-332 ◽  
Author(s):  
S.Y. Li ◽  
I.S. Otvos
Keyword(s):  
Lymantria Dispar ◽  
Nuclear Polyhedrosis Virus ◽  
Choristoneura Fumiferana ◽  
Choristoneura Occidentalis ◽  
Optical Brightener ◽  
Host System ◽  
Viral Activity ◽  
Optical Brighteners ◽  
Activity Enhancement ◽  
Nuclear Polyhedrosis

Optical brighteners were recently discovered to function as UV protectants for baculoviruses of insects (Shapiro 1992) and, also, to enhance entomopathogenic activity of these viruses (Hamm and Shapiro 1992; Li and Otvos 1999a). Enhancement of viral activity by brighteners varied greatly from one virus–host system to another: for example, from 1500-fold in LdMNPV against Lymantria dispar (L.) (Lepidoptera: Lymantriidae) (Argauer and Shapiro 1997) to 2- to 13-fold in a multicapsid nuclear polyhedrosis virus of Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae) (CfMNPV) against Choristoneura occidentalis Freeman (Li and Otvos 1999a). The reason for this variation in enhancement of viral activity is still unknown. In this study, we used CfMNPV to compare the levels of viral enhancement by an optical brightener between C. occidentalis and C. fumiferana.

Enhancement in Activity of Homologous and Heterologous Baculoviruses Infectious to Fall Armyworm (Lepidoptera: Noctuidae) by Selected Optical Brighteners

1999 ◽  
Vol 34 (4) ◽  
pp. 381-390 ◽  
Author(s):  
Martin Shapiro ◽  
John J. Hamm
Keyword(s):  
Galleria Mellonella ◽  
Fall Armyworm ◽  
Optical Brightener ◽  
Active Virus ◽  
Optical Brighteners ◽  
Nuclear Polyhedrosis ◽  
Tinopal Lpw ◽  
Anagrapha Falcifera ◽  
Alfalfa Looper ◽  
Lepidoptera Noctuidae

The nuclear polyhedrosis virus (NPV) from Spodoptera frugiperda (J. E. Smith) (SfMNPV) was the most active virus tested against fall armyworm, larvae (LC50 = 8.1 PIB per mm2). No LC 50s could be obtained for the alfalfa looper, Autographa californica (Speyer), NPV (AcMNPV), the celery looper, Anagrapha falcifera (Kirby), NPV (AfMNPV), the wax moth, Galleria mellonella (L.), NPV (GmMNPV), or the bollworm, Helicoverpa armigera (Hübner), NPV (HaMNPV). The addition of an optical brightener, Tinopal LPW® (1%), (Sigma Co., St. Louis, MO) significantly enhanced the activities of all NPVs. The most activie NPV/Tinopal LPW combination was SfMNPV, followed by AcMNPVm HaMNPV, AfMNPV, and GmMNPV. In terms of speed of kill, SfMNPV was the most active virus tested. When Tinopal LPW was added, the LT50 was reduced by more than 35%. The addition of Tinopal LPW to the heterologous NPVs resulted in LC50 and LT50 values that were comparable to SfMNPV alone. Five of eight brighteners acted as activity enhancers for SfMNPV (i.e., Blankophor BBH, Blankophor HRS, Blankophor P167, Blankophor RKH, and Tinopal LPW), whereas Blankophor BSU, Blankophor DML, and Blankophor LPG did not enhance virus activity.

scholarly journals Identification of sex-linked markers in the sexually cryptic coco de mer: are males and females produced in equal proportions?

AoB Plants ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Emma J Morgan ◽  
Christopher N Kaiser-Bunbury ◽  
Peter J Edwards ◽  
Mathias Scharmann ◽  
Alex Widmer ◽  
...  
Keyword(s):  
Sex Ratios ◽  
Differential Mortality ◽  
Sexually Dimorphic ◽  
Male And Female ◽  
Determination System ◽  
Males And Females ◽  
Heterogametic Sex ◽  
Sex Determination System ◽  
Male Specific ◽  
Genetically Determined

Abstract Lodoicea maldivica (coco de mer) is a long-lived dioecious palm in which male and female plants are visually indistinguishable when immature, only becoming sexually dimorphic as adults, which in natural forest can take as much as 50 years. Most adult populations in the Seychelles exhibit biased sex ratios, but it is unknown whether this is due to different proportions of male and female plants being produced or to differential mortality. In this study, we developed sex-linked markers in Lodoicea using ddRAD sequencing, enabling us to reliably determine the gender of immature individuals. We screened 589 immature individuals to explore sex ratios across life stages in Lodoicea. The two sex-specific markers resulted in the amplification of male-specific bands (Lm123977 at 405 bp and Lm435135 at 130 bp). Our study of four sub-populations of Lodoicea on the islands of Praslin and Curieuse revealed that the two sexes were produced in approximately equal numbers, with no significant deviation from a 1:1 ratio before the adult stage. We conclude that sex in Lodoicea is genetically determined, suggesting that Lodoicea has a chromosomal sex determination system in which males are the heterogametic sex (XY) and females are homogametic (XX). We discuss the potential causes for observed biased sex ratios in adult populations, and the implications of our results for the life history, ecology and conservation management of Lodoicea.

GONADOTROPHIN PATTERNS IN MALE AND FEMALE RATS:

1968 ◽  
Vol 58 (4) ◽  
pp. 600-612 ◽  
Author(s):  
Robert Boyd ◽  
Donald C. Johnson
Keyword(s):  
Ascorbic Acid ◽  
Testosterone Propionate ◽  
Female Rats ◽  
Female Rat ◽  
Feedback Effects ◽  
Male And Female ◽  
Prostate Weight ◽  
Male And Female Rats ◽  
Males And Females ◽  
Normal Males

ABSTRACT The effects of various doses of testosterone propionate (TP) upon the release of luteinizing hormone (LH or ICSH) from the hypophysis of a gonadectomized male or female rat were compared. Prostate weight in hypophysectomized male parabiotic partners was used to evaluate the quantity of circulating LH. Hypophyseal LH was measured by the ovarian ascorbic acid depletion method. Males castrated when 45 days old secreted significantly more LH and had three times the amount of pituitary LH as ovariectomized females. Administration of 25 μg TP daily reduced the amount of LH in the plasma, and increased the amount in the pituitary gland, in both sexes. Treatment with 50 μg caused a further reduction in plasma LH in males, but not in females, while pituitary levels in both were equal to that of their respective controls. LH fell to the same low level in partners of males or females receiving 100 μg TP. When gonadectomized at 39 days, males and females had the same amount of plasma LH, but males had more stored hormone. Pituitary levels were unchanged from controls following treatment with 12.5, 25 or 50 μg TP daily, but plasma values dropped an equal amount in both sexes with the latter two doses. Androgenized males or females, gonadectomized when 39 days old, were very sensitive to the effects of TP and plasma LH was significantly reduced with 12.5 μg daily. Pituitary LH in androgenized males was higher than that of normal males but was reduced to normal by small amounts of TP. The amount of stored LH in androgenized females was not different from that of normal females and it was unchanged by any dose of TP tested. Results are consistent with the conclusion that the male hypothalamic-hypophyseal axis is at least as sensitive as the female axis to the negative feedback effects of TP. Androgenization increases the sensitivity to TP in both males and females.

Gender Differences in Clothing Worn in Current Popular Comic Books

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Kyle Landon Jossy
Keyword(s):  
Gender Differences ◽  
Content Analysis ◽  
Comic Books ◽  
Book Industry ◽  
Comic Book ◽  
Lower Body ◽  
Female Characters ◽  
Male And Female ◽  
Males And Females

This study looked at how males and females were portrayed, based on the amount of skin shown in the clothing worn.  A Content analysis was performed on a sample of 20 randomly selected popular comics from the last 3 years.  Both male and female characters were rated on how much skin they showed in three clothing categories; neck line, sleeve length, and lower body.  Results showed that in all 3 categories, women consistently wore more revealing clothing.  The findings demonstraetd that the comic book industry is comparable to other forms of media, in the sexualization of female characters, by having them wear more revealing clothing.

scholarly journals Spinal Muscular Atrophy after Nusinersen Therapy: Improved Physiology in Pediatric Patients with No Significant Change in Urine, Serum, and Liquor 1H-NMR Metabolomes in Comparison to an Age-Matched, Healthy Cohort

Metabolites ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 206
Author(s):  
Leon Deutsch ◽  
Damjan Osredkar ◽  
Janez Plavec ◽  
Blaž Stres
Keyword(s):  
Machine Learning ◽  
Spinal Muscular Atrophy ◽  
1H Nmr ◽  
Pediatric Patients ◽  
Muscular Atrophy ◽  
Male And Female ◽  
Before And After ◽  
Healthy Cohort ◽  
Males And Females ◽  
Urine Serum

Spinal muscular atrophy (SMA) is a genetically heterogeneous group of rare neuromuscular diseases and was until recently the most common genetic cause of death in children. The effects of 2-month nusinersen therapy on urine, serum, and liquor 1H-NMR metabolomes in SMA males and females were not explored yet, especially not in comparison to the urine 1H-NMR metabolomes of matching male and female cohorts. In this prospective, single-centered study, urine, serum, and liquor samples were collected from 25 male and female pediatric patients with SMA before and after 2 months of nusinersen therapy and urine samples from a matching healthy cohort (n = 125). Nusinersen intrathecal application was the first therapy for the treatment of SMA by the Food and Drug Administration (FDA) and the European Medicines Agency (EMA). Metabolomes were analyzed using targeted metabolomics utilizing 600 MHz 1H-NMR, parametric and nonparametric multivariate statistical analyses, machine learning, and modeling. Medical assessment before and after nusinersen therapy showed significant improvements of movement, posture, and strength according to various medical tests. No significant differences were found in metabolomes before and after nusinersen therapy in urine, serum, and liquor samples using an ensemble of statistical and machine learning approaches. In comparison to a healthy cohort, 1H-NMR metabolomes of SMA patients contained a reduced number and concentration of urine metabolites and differed significantly between males and females as well. Significantly larger data scatter was observed for SMA patients in comparison to matched healthy controls. Machine learning confirmed urinary creatinine as the most significant, distinguishing SMA patients from the healthy cohort. The positive effects of nusinersen therapy clearly preceded or took place devoid of significant rearrangements in the 1H-NMR metabolomic makeup of serum, urine, and liquor. Urine creatinine was successful at distinguishing SMA patients from the matched healthy cohort, which is a simple systemic novelty linking creatinine and SMA to the physiology of inactivity and diabetes, and it facilitates the monitoring of SMA disease in pediatric patients through non-invasive urine collection.

scholarly journals Stem cell treatment improves post stroke neurological outcomes: a comparative study in male and female rats

2021 ◽  
pp. svn-2020-000834
Author(s):  
Koteswara Rao Nalamolu ◽  
Bharath Chelluboina ◽  
Casimir A Fornal ◽  
Siva Reddy Challa ◽  
David M Pinson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sex Differences ◽  
Motor Function ◽  
Infarct Volume ◽  
Female Rats ◽  
Human Umbilical Cord ◽  
Male And Female ◽  
Post Stroke ◽  
Male And Female Rats ◽  
Males And Females

Background and purposeThe therapeutic potential of different stem cells for ischaemic stroke treatment is intriguing and somewhat controversial. Recent results from our laboratory have demonstrated the potential benefits of human umbilical cord blood-derived mesenchymal stem cells (MSC) in a rodent stroke model. We hypothesised that MSC treatment would effectively promote the recovery of sensory and motor function in both males and females, despite any apparent sex differences in post stroke brain injury.MethodsTransient focal cerebral ischaemia was induced in adult Sprague-Dawley rats by occlusion of the middle cerebral artery. Following the procedure, male and female rats of the untreated group were euthanised 1 day after reperfusion and their brains were used to estimate the resulting infarct volume and tissue swelling. Additional groups of stroke-induced male and female rats were treated with MSC or vehicle and were subsequently subjected to a battery of standard neurological/neurobehavioral tests (Modified Neurological Severity Score assessment, adhesive tape removal, beam walk and rotarod). The tests were administered at regular intervals (at days 1, 3, 5, 7 and 14) after reperfusion to determine the time course of neurological and functional recovery after stroke.ResultsThe infarct volume and extent of swelling of the ischaemic brain were similar in males and females. Despite similar pathological stroke lesions, the clinical manifestations of stroke were more pronounced in males than females, as indicated by the neurological scores and other tests. MSC treatment significantly improved the recovery of sensory and motor function in both sexes, and it demonstrated efficacy in both moderate stroke (females) and severe stroke (males).ConclusionsDespite sex differences in the severity of post stroke outcomes, MSC treatment promoted the recovery of sensory and motor function in male and female rats, suggesting that it may be a promising treatment for stroke.

Mammalian exocrine secretions XIX. Chemical characterization of the interdigital secretion of the Black Wildebeest, Connochaetes gnou

2020 ◽  
Vol 75 (9-10) ◽  
pp. 339-351
Author(s):  
Barend V. Burger ◽  
Desmond Slade ◽  
Marlize Z. Bekker ◽  
Aron H. Goitom
Keyword(s):  
Chemical Characterization ◽  
Male And Female ◽  
Black Wildebeest ◽  
Connochaetes Gnou ◽  
Electron Impact Mass ◽  
Males And Females ◽  
Strong Attachment ◽  
Impact Mass ◽  
Acid Esters

AbstractUsing gas chromatography (GC) in conjunction with electron impact mass spectrometry and retention-time comparison, 94 compounds, ranging from 2-methyl-2-propenal to octadecanoic acid, were identified in the interdigital secretions of male and female black wildebeests, Connochaetes gnou (also known as the white-tailed gnu). The constituents of these secretions belong to many different compound classes, including hydrocarbons, alcohols, aromatics and aliphatic carbonyl compounds including carboxylic acids as well as carboxylic acid esters. Relatively small quantitative differences were found between the male and female interdigital secretions. It was concluded that these compounds probably do not play a significant role in territorial marking or in chemical communication between males and females of the species, but they could be involved in preserving the remarkably strong attachment between members of social subgroups in black wildebeest populations.

scholarly journals Inter-Limb Jump Asymmetries and Their Association with Sport-Specific Performance in Young Male and Female Swimmers

2021 ◽  
Vol 18 (14) ◽  
pp. 7324
Author(s):  
Max I. Phukan ◽  
Rohit K. Thapa ◽  
Gopal Kumar ◽  
Chris Bishop ◽  
Helmi Chaabene ◽  
...  
Keyword(s):  
National Level ◽  
Young Male ◽  
Front Crawl ◽  
Male And Female ◽  
Standing Long Jump ◽  
Significant Difference ◽  
Specific Performance ◽  
Long Jump ◽  
Males And Females ◽  
Level Of Agreement

This study aimed to examine inter-limb jump asymmetries and their association with sport-specific performance in young swimmers. Thirty-eight (male, n = 19; female, n = 19) regional/national level young swimmers (age: 12.3 ± 1.2 years; height: 159.6 ± 8.2 cm; body mass: 52.5 ± 9.2 kg) participated in this study. Inter-limb asymmetries were assessed for single-leg countermovement jump (SLCMJ) and single-leg standing long jump (SLSLJ). Sport-specific performance was evaluated using front crawl (i.e., 50 m and 25 m) and front crawl kick (i.e., 50 m and 25 m). The kappa coefficient revealed a “slight” level of agreement (Κ = 0.156, 0.184, and 0.197 for female, male, and all, respectively) between the direction of asymmetry for SLCMJ and SLSLJ, indicating that asymmetries rarely favored the same limb during both tests. A paired sample t-test showed a significant difference (p = 0.025) between asymmetry scores obtained in SLCMJ and SLSLJ. No significant difference was found in asymmetry scores between males and females (p = 0.099 to 0.977). Additionally, no association between asymmetry scores and sport-specific performance was observed (p > 0.05). Our findings highlight the independent nature of inter-limb asymmetries derived from SLCMJ and SLSLJ among young male and female swimmers. Further, our results suggest no association between jumping asymmetries and sport-specific performance.

Profile of a short-acting κ-antagonist, LY2795050, on self-grooming behaviors, forced swim test and locomotor activity: sex comparison in mice

2021 ◽  
pp. 026988112199688
Author(s):  
Eduardo R Butelman ◽  
Caroline Baynard ◽  
Bryan D McElroy ◽  
Thomas E Prisinzano ◽  
Mary Jeanne Kreek
Keyword(s):  
Locomotor Activity ◽  
Forced Swim Test ◽  
Male And Female ◽  
Short Acting ◽  
Swim Test ◽  
Central Nervous System Dysfunction ◽  
Forced Swim ◽  
Males And Females ◽  
The Impact ◽  
Κ Receptor

Background: Novel short-acting κ(kappa)-opioid receptor selective antagonists are translational tools to examine the impact of the κ-receptor/dynorphin system in assays related to central nervous system dysfunction (e.g., substance use disorders, anhedonia and depression). The effects of such compounds have been compared in males and females under very limited conditions. Aims: The goal of this study was to examine potential sex differences in the effects of a κ-agonist and a short-acting κ-antagonist in an ethologically relevant test of anhedonia, the “splash test” of self-grooming, and also in the forced swim test and in locomotor activity. Methods: We examined the dose-dependence of grooming deficits caused by the κ-agonist U50,488 (0.1–3.2 mg/kg intraperitoneal (i.p.)) in gonadally intact adult male and female C57BL/6J mice. We then compared the effects of the short-acting κ-antagonist LY2795050 ((3-chloro-4-(4-(((2S)-2-pyridin-3-ylpyrrolidin-1-yl)methyl) phenoxy)benzamide)); 0.032–0.1 mg/kg i.p.) in blocking grooming deficits caused by U50,488 (3.2 mg/kg). The effects of LY2795050 were also studied in the forced swim test (FST). The effects of LY2795050 in blocking the locomotor depressant effects of U50,488 (10 mg/kg) were also studied. Results: U50,488 produced dose-dependent grooming deficits in male and female mice, and LY2795050 prevented these effects. In contrast, LY2795050 decreased immobility in the FST in males at a dose of 0.1 mg/kg, but not in females, up to a dose of 0.32 mg/kg. Also, LY2795050 (0.32 mg/kg) prevented and also reversed the locomotor-depressant effects of U50,488 (10 mg/kg), in males and females. Conclusions: This study further implicates the κ-receptor system in ethologically relevant aspects of anhedonia, and confirms sexual dimorphism in some behavioral effects of novel κ-antagonists.

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